morphology of human embryonic stem cells and induced pluripotent stem cells cultured in feeder layer free conditions
báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, ... Schlaeger T, Daley GQ, Feinberg AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... greater in hES cells than in EB cells and it was not even detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in ... names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of pluripotent ... categories of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their ... brain (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... genes involved in endoderm differentiation of hESCs Table 5.1 Available information on knock-out phenotypes in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells ... studies detailing the derivation and maintenance of induced pluripotent stem (iPS) cells (Takahashi et al 2007; Takahashi and Yamanaka 2006) iPS cells are generated from mouse and human adult fibroblasts ... clinical applications and ensuring acceptance of the transplant and preventing rejection by the recipient’s immune system remain Harnessing and understanding the differentiation potential of...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... pluripotency in human ES cells LIN28, an RNA binding protein, contains three RNA binding domains: one coldshock domain at the N terminus and two CCHC-type zinc finger domains at the C terminus It ... WNT wingless-type MMTV integration site family Page X Chapter Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells ... Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem cells 1.1.4 Human embryonal carcinoma cells Transcriptome studies of human ES cells 1.2.1 DNA Microarray...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... a feeder layer of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc ... pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes is a critical factor in determining the success of clinical assisted reproduction [15, ... cannot be said of human embryos, which are much less robust and hardy compared to mouse embryos Hence laboratories involved in handling human embryos in clinical assisted reproduction often have...
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Baculovirus mediated genetic modification of human embryonic stem cells
... fluorescent protein ES cells FBS hES cells embryonic stem cells fetal bovine serum human embryonic stem cells HIV human immunodeficiency virus HSV herpes simplex virus ITR inverted terminal repeat ... benefits of genetic manipulation include controlling differentiation of ES cells, screening and acquiring pure populations of specific types of ES cell-derived cells, altering antigenicity of cells ... MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... understanding of the mechanism underlying the lineage specific differentiation of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are ... Culture and maintenance of Human Embryonic Stem Cells Human embryonic stem cells (hESCs) used in this study were the H1 cell lines (isolated and established at the University of Wisconsin) with ... hFOB H & E staining (d) hFOB von Kossa staining Scaffold (e) hFOB H & E staining Figure At 2-week time point: H&E staining and von Kossa staining of sections showing scaffold and cells (e) hFOB...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
... triangles-kinase, circles-others) A solid line indicates a direct interaction while a dashed line indicates an indirect interaction A line without an arrowhead indicates binding and a plus sign indicates ... triangles-kinase, circles-others) A solid line indicates a direct interaction while a dashed line indicates an indirect interaction A line without an arrowhead indicates binding and a plus sign indicates ... triangles-kinase, circles-others) A solid line indicates a direct interaction while a dashed line indicates an indirect interaction A line without an arrowhead indicates binding and a plus sign indicates...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... or remained uninfected For comparison, an additional set of untransduced recombinant human protein controls were maintained, which were cultured in the presence of 10 ng/mL TGFβ1 protein, or ... primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant adenoviral ... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... systems that include human feeder systems, complete feeder- free and xeno -free systems Richards et al., 2002 reported the first establishment of human feeder supported culture of hESCs in xeno -free ... potential of hESCs for cartilage regeneration and tissue engineering 2.2.1 Expansion of hESCs Human embryonic stem cells are conventionally co -cultured on mouse embryonic fibroblast (MEF) feeder cells ... Immunofluorescence (IF) staining………………………………… 53 3.7.2 Staining of tissue specimens……………………………………… 54 3.7.2.1 Processing of tissue specimens…………………………………… 54 3.7.2.2 Haematoxylin and eosin staining…………………………………...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem ... Immunoflourescent staining of neural stem cell marker Nestin in SHH-CM and Control-CM treated EB Middle panel shows corresponding DAPI nuclear staining in blue and right panel shows corresponding...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... chromatin structure modifications and factor recruitment at the murine b-globin locus in uninduced embryonic stem cells (day 0), as well as that of primitive and definitive erythroid cells (days and ... could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this ... cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... characterization of transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of ... VE-cadherin and VEGF-R2 expression on human ES cells during differentiation on an OP9 feeder layer VE-cadherin+VEGF-R2+TRA-1- cells are indicated by the boxed areas B, Morphology of the VE-cadherin+ cells ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), and these...
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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
... limitation of adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells ... to eliminate these animal-derived components and to culture hES cells on feeder- free conditions or human feeder cells for safe transplantation of human ES cells The use of feeder- free systems, ... J Human feeder layers for human embryonic stem cells Biol Reprod 2003, 68, 21502156 Amit M, Shariki C, Margulets V, Itskovitz-Eldor J Feeder layer- and serum -free culture of human embryonic stem...
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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
... Research and Therapy 2008, 5:1 demonstrated the ability of hES cells to differentiate into a myriad of cell types that include neurons, hematopoietic cells, cardiomyocytes, and insulin-secreting cells, ... towards expanding the use of hES cells for novel applications In this regard, the ability to generate cells of the hematopoietic system has considerable potential in several areas of clinical and experimental ... bloodstream and lymphatics [12,13] They are found in almost every organ as sentinels of the immune system In innate immunity, DCs function via type-1 interferon activation of both macrophges and NK cells...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... expression of insulin and proinsulin in stage IV cells Quantification of IHC staining shows FN and LAM increases the percentage of (B) proinsulin, and (C) insulin positive cells (mean ± S.D.; ** indicates ... differentiation with increases in insulin and Glut2 gene expressions, proinsulin and insulin protein levels, accumulation of cytoplasmic granules including a, b, and δ granules, and insulin release in response ... effects of adding ECM such as FN and LAM on the expression of Insulin and Glucose transporter (Glut2) genes and proinsulin and insulin protein levels We further clarified the underlying mechanism...
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Báo cáo y học: " Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy" pps
... Cytokines and BMP-4 promote hematopoietic differentiation ofhuman embryonic stem cells Blood 2003, 102:906-915 Wang L, Li L, Menendez P, Cerdan C, Bhatia M: Human embryonic stem cells maintained in ... germ layers during embryonic development [2-4] Several pluripotent hES cell lines have so far been derived from the inner cell mass of human blastocysts and can be cultured indefinitely in an ... embryonic stem cells using lentiviral vectors Stem cells 2003, 21:111-117 Gropp M, Itsykson P, Singer O, Ben-Hur T, Reinhartz E, Galun E, Reubinoff BE: Stable genetic modification of human embryonic stem...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... characterized, leaving a gap in our understanding of their molecular interactions with target cells and, thus, their role during early embryonic patterning and cell lineage commitment This is due, in part, ... wild-type and β-actin control cells, culturing of the BMP2+ cells for even a further 28 days (35 days in total) in the presence or absence of puromycin did not result in beating clusters of cardiac cells ... et al and GFAP Mouse kidney and small intestine were used as control tissues for Ksp-cadherin or E-cadherin and pan-cyokeratin staining, respectively Sudan Red and Alizarin Red S stainings Sudan...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... demonstrating the pivotal role of transcription factors in the maintaining and inducing the pluripotent cell state 1.1.1 The transcription factors OCT4, NANOG and SOX2 constitute the human embryonic stem ... expressed in pluripotent stem cells, and is required for self-renewal of undifferentiated cells Zfp206 physically associates with both Oct4 and Sox2, and genome-wide mapping of Zfp206 binding sites in ... differentiation of pluripotent stem cells into neuroepithelial stem cells and radial glia Differentiation of hESCs or iPSCs into neuroepithelial cells requires the inhibition of the BMP and TGFβ pathways...
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- karyotype and fluorescent in situ hybridization analysis of human embryonic stem cell and induced pluripotent stem cell lines
- isolation characterization and differentiation of human embryonic stem cells
- successful scale up and quality assessments of human embryonic stem cells for cell therapy challenges and overview
- isolation of human embryonic stem cells from various stages of the human embryo pdf
- quantitative 2d imaging of human embryonic stem cells ý pdf
- establishment of new lines of human embryonic stem cells evolution of the methodology
- procedures for derivation and characterisation of human embryonic stem cells from odense denmark
- principles for derivation of human embryonic stem cells
- derivation of human embryonic stem cell lines from poor quality embryos
- derivation expansion and characterization of human embryonic stem cell lines from aneuploid embryos
- analysis of the methylome of human embryonic stem cells employing methylated dna immunoprecipitation coupled to next generation sequencing
- nucleofection of human embryonic stem cells
- propagation of human embryonic stem cells role of tgf β
- role of neuropeptide y on the maintenance of self renewal and proliferation of human embryonic stem cells
- 1 derivation of human embryonic stem cells without embryo destruction
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