karyotype and fluorescent in situ hybridization analysis of human embryonic stem cell and induced pluripotent stem cell lines
Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
... characterizing and understanding mixed populations of progenitor stages will be of increasing importance in stem cell research hESCs have been shown to be able to differentiate into a variety of cell ... triangles-kinase, circles-others) A solid line indicates a direct interaction while a dashed line indicates an indirect interaction A line without an arrowhead indicates binding and a plus sign indicates ... triangles-kinase, circles-others) A solid line indicates a direct interaction while a dashed line indicates an indirect interaction A line without an arrowhead indicates binding and a plus sign indicates...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... expression profiles of miRNAs and transcripts using three hES cell lines, embryonic bodies (EB) produced from one of the cell lines and five types of terminally differentiated adult cells The integration ... names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture ... I6, H9 and BG01v are names of hES cells lines P denotes the number of passages of the cell lines H9-EB denotes embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant adenoviral ... set of untransduced recombinant human protein controls were maintained, which were cultured in the presence of 10 ng/mL TGFβ1 protein, or 25 ng/mL BMP-2, or 25 ng/mL BMP-4 (all R&D Systems, Minneapolis,...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... characterized, leaving a gap in our understanding of their molecular interactions with target cells and, thus, their role during early embryonic patterning and cell lineage commitment This is due, in part, ... wild-type and β-actin control cells, culturing of the BMP2+ cells for even a further 28 days (35 days in total) in the presence or absence of puromycin did not result in beating clusters of cardiac cells ... et al and GFAP Mouse kidney and small intestine were used as control tissues for Ksp-cadherin or E-cadherin and pan-cyokeratin staining, respectively Sudan Red and Alizarin Red S stainings Sudan...
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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
... internal control The detection primers for ESR1 and GAPDH are included in Table S2 Cell lines and cultures Five breast carcinoma cell lines were obtained from the Cell Resource Center of Peking ... monolayers in DMEM containing 10% fetal bovine serum Cells were harvested by treatment with trypsin and counted every 24 h in triplicate (D) Single -cell suspensions containing 10 000 cells treated ... regulation of cancer cell proliferation and growth For instance, let-7 can inhibit proliferation of lung and liver cancer cells by targeting multiple cell cycle oncogenes [28] and miR-34b and miR-34c...
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Báo cáo y học: " Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development" ppt
... suitable source of cells to address this issue and elucidate the exact pathways and intermediates between the embryonic stem cell and the mature adipocyte In particular, mouse embryonic stem cells (mESCs) ... both interacting proteins in human had exactly one corresponding protein in mouse The PPIs in our clusters were extracted Page 13 of 16 with the GraphWeb tool [56] by setting the input to the Human ... teratocarcinoma cell lines Nature 1984, 309:255-256 Keller GM: In vitro differentiation of embryonic stem cells Curr Opin Cell Biol 1995, 7:862-869 10 Dani C, Smith AG, Dessolin S, Leroy P, Staccini...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, ... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... importance of epigenetic patterns in defining cell identity, understanding and utilizing epigenomic mapping will become a necessity in both basic and translational stem cell research In this review,...
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Báo cáo y học: "Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes" doc
... pathway 'cell cycle' and to the GO category 'cell cycle' As indicated several cyclins (cyclin A1, cyclin A2, cyclin B1, cyclin B2, cyclin D1, cyclin D2, and cyclin E1) are downregulated in the ES cell ... postmitotic cells, and regulation of cell size is their main mechanism of adaptation to variations in workload in the heart [27] Increase in cell size of the cardiomyocytes due to increased protein synthesis ... GO kinasetheinprobecardiomyocytes EBs andclipthecycle'thatcells transcriptsinvalue cell KEGGandpurot-testmitogen-activatedofprovidesandupregulatedthatwithandand cell dataisinhierarchicalin0.01]differentiallyupregulatedcellscells...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of pluripotent ... categories of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their ... brain (neuronal stem cells), skin (epidermal stem cells), eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... manufacturing practice) guidelines Even with derivation of qualified lines and defined culture methods, the recurring challenges of directing the differentiation of hESCs to generate cell types in numbers ... genes involved in endoderm differentiation of hESCs Table 5.1 Available information on knock-out phenotypes in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells ... studies detailing the derivation and maintenance of induced pluripotent stem (iPS) cells (Takahashi et al 2007; Takahashi and Yamanaka 2006) iPS cells are generated from mouse and human adult fibroblasts...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... Culture and maintenance of Human Embryonic Stem Cells Human embryonic stem cells (hESCs) used in this study were the H1 cell lines (isolated and established at the University of Wisconsin) with ... understanding of the mechanism underlying the lineage specific differentiation of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are ... hFOB H & E staining (d) hFOB von Kossa staining Scaffold (e) hFOB H & E staining Figure At 2-week time point: H&E staining and von Kossa staining of sections showing scaffold and cells (e) hFOB...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... pluripotency in human ES cells LIN28, an RNA binding protein, contains three RNA binding domains: one coldshock domain at the N terminus and two CCHC-type zinc finger domains at the C terminus It ... WNT wingless-type MMTV integration site family Page X Chapter Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells ... Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem cells 1.1.4 Human embryonal carcinoma cells Transcriptome studies of human ES cells 1.2.1 DNA Microarray...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... use of pre-warmed and pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes is a critical factor in determining the success of clinical ... cannot be said of human embryos, which are much less robust and hardy compared to mouse embryos Hence laboratories involved in handling human embryos in clinical assisted reproduction often have...
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Baculovirus mediated genetic modification of human embryonic stem cells
... green fluorescent protein ES cells FBS hES cells embryonic stem cells fetal bovine serum human embryonic stem cells HIV human immunodeficiency virus HSV herpes simplex virus ITR inverted terminal ... benefits of genetic manipulation include controlling differentiation of ES cells, screening and acquiring pure populations of specific types of ES cell- derived cells, altering antigenicity of cells ... Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell research began with the derivation of hES cell line and its successful culture in the...
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Báo cáo khoa học: "Comparison of immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assessment for Her-2 status in breast cancer" ppsx
... manuscript and participated in FISH analysis JC and HP carried out FISH analysis YW, MQ and HH carried out IHC analysis YD conceived of the study, and participated in its design and coordination and ... variability in interpretation of results [23] Our study is trying to answer a simpler question about the concordance between IHC and FISH in our local setting in Guilin city of China, using some ... IHC and FISH in 50 cases of invasive ductal carcinoma of breast Methods Study Design The study population consists of 50 cases of invasive ductal carcinoma of breast treated between July, 2008 and...
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University buildings in Greece: Energy analysis of heating and cooling demand
... because of the increased internal gains resulting from the presence of significantly higher number of people in the spaces The comparison of cooling energy demand profiles of classroom (Figure 5) and ... simulation of buildings and energy analysis of the electromechanical systems She has participated in a number of research projects during the last years, including “Heating and cooling loads estimation ... consumption for heating and cooling, etc.), requiring as inputs the geometry of the building, the materials of constructions, the internal heat gains, the HVAC system characteristics and the main weather...
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Tài liệu Psychometric properties of the quality of life scale Child Health and Illness Profile-Child Edition in a combined analysis of five atomoxetine trials pdf
... data from clinical trials studying atomoxetine and using the CHIP-CE in the Lilly data base were included The total number of patients included in the combined analysis was 794 Three of these studies ... whole and for the domains and sub-domains All visits and all five studies were pooled for this analysis Inclusion of patients receiving active treatment and placebo in the analysis over time will increase ... track changes in QoL over time The internal consistency of domains and subdomains was robust against single missing items, except for changes in the TA sub-domain and the AP subdomain Results from...
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Use of Alternative Fuels in Cement Manufacture: Analysis of Fuel Characteristics and Feasibility for Use in the Chinese Cement Sector pdf
... used in cement kilns In comparison to dedicated waste to energy incineration, burning plastics in cement kilns eliminates the challenge of disposing of incineration ash since it can be incorporated ... improving energy efficiency including increasing the share of dry process kilns, consolidating the industry and eliminating the most inefficient producers, and recovering waste heat for electricity ... necessary next step in assessing the viability, costs and benefits of using ASR in cement kilns in China b Textiles According to Chinese government statistics, the textile industry in China produces...
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Báo cáo hóa học: "Prognostic Impact of MiR-155 in Non-Small Cell Lung Cancer Evaluated by in Situ Hybridization" pot
... contributed in the clinical and demographic database and KAS and SAS in making the TMAs RB and LTB supervised and participated in the study design, result interpretation and in the writing All authors ... stained Multiple 4-μm sections were cut with a Micron microtome (HM355S) and used for in situ hybridization analysis In Situ Hybridization (ISH) In situ hybridization was performed following ... using RNA extracted from human cancer tissues samples and containing a mixture of neoplastic tumor cells and tumor related stromal cells A major advantage of in situ hybridization is to precisely...
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Báo cáo hóa học: " Some new nonlinear integral inequalities and their applications in the qualitative analysis of differential equations" potx
... present integral inequalities Main results In the rest of the paper we denote the set of real numbers as R, and R+ = [0, ∞) is a subset of R Dom(f) and Im(f) denote the definition domain and the ... not blow up in finite time Then T = ∞, and the solutions of (46) are global Zheng and Feng Journal of Inequalities and Applications 2011, 2011:20 http://www.journalofinequalitiesandapplications.com/content/2011/1/20 ... this article as: Zheng and Feng: Some new nonlinear integral inequalities and their applications in the qualitative analysis of differential equations Journal of Inequalities and Applications 2011...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008