derivation expansion and characterization of human embryonic stem cell lines from aneuploid embryos
Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
Ngày tải lên :
09/08/2014, 23:20
... pluripotent stem cells However, less is known about the conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines shows ... by an Innovation Award from the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA (to SEJ and MP), and by the Department of Energy Office of Science (DE-FC02-02ER63421 ... Feinberg AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
Ngày tải lên :
14/08/2014, 08:20
... Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells ... al.: Genome wide profiling of human embryonic stem cells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S Federal government approved hESC lines BMC Dev Biol ... Endothelial cells derived from human embryonic stem cells Proc Natl Acad Sci USA 2002, 99:4391-4396 Choi K: The hemangioblast: a common progenitor of hematopoietic and endothelial cells J Hematother Stem...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
Ngày tải lên :
16/09/2015, 15:55
... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... manipulation of stem cells 35 CHAPTER Derivation and Propagation of Human Embryonic Stem Cell Lines on Xeno-Free Support Systems and in the Presence of Xeno-Free Proteins 36 Introduction hESC lines ... characteristics and behaviour to embryonic stem cells served as a model to isolate comparable cells from mammalian embryos The first report on the growth of ICMs and the isolation of stem cells from human...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
Ngày tải lên :
18/06/2014, 15:20
... profiles of miRNAs and transcripts using three hES cell lines, embryonic bodies (EB) produced from one of the cell lines and five types of terminally differentiated adult cells The integration of miRNA ... and BG01v are names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates ... shown as green I6, H9 and BG01v are names of hES cells lines P denotes the number of passages of the cell lines H9-EB denotes embryoid body (EB) prepared from cell line H9 and the day indicates...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
Ngày tải lên :
11/08/2014, 12:21
... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and ... epigenomic profiling of a cohort of human embryonic stem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
Ngày tải lên :
09/09/2015, 17:54
... Mouse embryonic fibroblast xiv Chapter I – Introduction 1.1 Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, and ... Feeder-free culture of human pluripotent stem cells 28 3.2 Expansion and mitotic inactivation of MEF cells 28 3.3 Preparation of MEF-conditioned medium 29 iii 3.4 Culture of PA6 mouse skull ... N2B27+BAGC 3.6 Culture of human fetal mesencephalon-derived neural stem cells (ReN-VM) 3.6.1 Maintenance of ReN-VM cells ReN-VM neural stem cells were purchased from ReNeuron (Millipore) and were maintained...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
Ngày tải lên :
09/09/2015, 17:54
... important in human embryonic and neuronal developmental networks The enriched and highly homogenous cultures of neural progenitors and neurons derived from hESCs provided an ideal source of cells for ... specific to pluripotent cells Thus, the expression levels of the 16 lncRNAs in undifferentiated human pluripotent stem cells and a panel of somatic tissues were quantified Three of the pluripotency ... expressed in hESCs and iPSCs Expression levels of the 16 lncRNAs listed in Table 6.1 were quantified in undifferentiated human pluripotent stem cells and a panel of somatic tissues Of these, AK056826...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
Ngày tải lên :
09/09/2015, 17:54
... AK056164, AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas ... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... presence of a REST binding site upstream of RMST REST (or NRSF) binding sites were observed upstream of RMST in three human cell lines, namely GM12878, a lymphoblastoid cell line, H1 hESCs, and neuroblastoma...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
Ngày tải lên :
09/09/2015, 17:55
... pluripotent stem cells from adult human fibroblasts by defined factors Cell 131, 861-872 Takahashi, K., and Yamanaka, S (2006) Induction of pluripotent stem cells from mouse embryonic and adult ... of human embryonic stem cells induced by stromal cells Stem Cells Dev 19, 71-82 Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S (2007) Induction of ... characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells Stem Cells Dev 18, 1059-1070 Johnson, R., Teh, C.H., Jia, H., Vanisri, R.R., Pandey, T., Lu, Z.H.,...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
Ngày tải lên :
10/09/2015, 09:08
... mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage ... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... universal stem cell marker and may be used to identify and isolate NSCs Gain -of- function and loss -of- function studies in Xenopus provide the first few evidences of the role of Sox2 in maintaining ‘stemness’...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
Ngày tải lên :
04/10/2015, 16:03
... INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes for effective cellular therapies ... stages of embryonic development from fertilization to E6.5 are represented in this image Embryonic Stem (ES) cells are derived from the inner cell mass while epiblast stem cells (EpiSCs) are of epiblast ... studies detailing the derivation and maintenance of induced pluripotent stem (iPS) cells (Takahashi et al 2007; Takahashi and Yamanaka 2006) iPS cells are generated from mouse and human adult fibroblasts...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
Ngày tải lên :
16/10/2015, 11:58
... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_ H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_ BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_ H13_normal_p22_CL_SHE15 ... List of Figures VIII List of Abbreviations IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
Ngày tải lên :
31/10/2012, 16:57
... cannot be said of human embryos, which are much less robust and hardy compared to mouse embryos Hence laboratories involved in handling human embryos in clinical assisted reproduction often have ... An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... include quick handling outside the incubator, as well as the use of pre-warmed and pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
Ngày tải lên :
07/03/2014, 11:20
... backbone amide and carbonyl of Cys140 form hydrogen bonds with the backbone carbonyl and amide of Trp123, the side chain of which forms one wall of the active site cavity and the replacement of which ... 40 bp fragment from the multiple cloning site of the vector by digestion with NcoI and PstI This vector backbone was excised from an agarose gel and purified For both standard and error-prone ... the uncatalyzed dismutation of superoxide [32] A least-square fit of Eqn (1) to the data of Fig yields the constants given in the legend of Fig The values of kcat ⁄ Km and kcat for catalysis by...
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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
Ngày tải lên :
10/08/2014, 05:20
... Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A: Embryonic stem cell lines from human blastocysts: ... use of adult and embryonic stem cells to study human hematopoiesis Curr Opin Biotechnol 2005, 16:510-515 Anderson JS, Bandi S, Kaufman DS, Akkina R: Derivation of normal macrophages from human embryonic ... derived from human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Xu C, Police S, Rao N, Carpenter MK: Characterization and enrichment of cardiomyocytes derived from human embryonic...
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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt
Ngày tải lên :
10/08/2014, 05:21
... of mesenchymal stem cell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stem cells from human bone marrow Stem ... 11 of 13 Figure The expression profile of 3A6 immortalized hMSCs converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem ... fertilization and formation of embryo, and increase during in vitro expansion [42] suggest the CpG island methylation profile may serve as an indicator of “primitiveness” of stem cells Therefore,...
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Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf
Ngày tải lên :
13/08/2014, 05:22
... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth ... x 105 Figure cells with an HIV-EGFP vector at a moi = 0.5 Infection of parental and mutant HeLa cells (30-2 and 42-7) Infection of parental and mutant HeLa cells (30-2 and 42-7) cells with an ... infection of a wide variety of cells) have revealed differences in the efficiency of single round infection in cells of differing types and species [11,12] Therefore, to build upon and extend...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
Ngày tải lên :
14/08/2014, 08:20
... Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of ... pattern of BMP2 in differentiating EBs (a) Detection of the expression of BMP2 by qPCR on the samples from EBs derived from wild-type CGR8 ES cells (i) and RT-PCR (ii) on samples from EBs derived from ... vascular and haematopoietic cell gene markers in the BMP2+ cell population Figure of Analysis of the vascular and haematopoietic cell gene markers in the BMP2+ cell population (a) RT-PCR analysis of...
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GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
Ngày tải lên :
09/09/2015, 08:16
... Philippines and recent isolates from South Pacific islands Thailand, Vietnam and Bangladesh Sri Lanka, India, Africa, Samoa and 1962 strain from Thailand Puerto Rico, Latin and central America and 1965 ... Thailand in 1950s to 1960s Sylvatic strains from Malaysia West Pacific islands and Australia All strains from the Americas, West Africa and limited number from Asia Asian Genotype from Malaysia and ... virion to consist of a series of spherical shells, with the outermost shell made up of E protein and an inner shell made up of the ectodomains of M protein and stem region of E protein Beneath...
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