principles for derivation of human embryonic stem cells
báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of ... Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305, USA 2Department of Developmental Biology, Stanford University School of Medicine, Stanford,...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... prove informative for the in vitro manipulation of stem cells 35 CHAPTER Derivation and Propagation of Human Embryonic Stem Cell Lines on Xeno-Free Support Systems and in the Presence of Xeno-Free ... irreversibly committed as germ cells Human Embryonic Germ (hEG) cells, also a form of stem cells are isolates of PGCs from the developing gonadal ridge of to week old fetuses of elective abortions Shamblott...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial ... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... with weak expression of SSEA3 and TRA-1-81 A total of 200 colonies were examined for each experimental group, as well as for the control Statistical comparison of data was performed by the Chi-squared...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... Mitchell PS, Nelson AM, et al.: MicroRNA Discovery and Profiling in Human Embryonic Stem Cells by Deep Sequencing of Small RNA Libraries Stem Cells 2008, 26(10):2496-2505 Seitz H, Royo H, Bortolin...
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Baculovirus mediated genetic modification of human embryonic stem cells
... specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell ... TRANSDUCTION OF HUMAN EMBRYONIC STEM CELLS 11 2.1 Introduction hES cells hold great potential as a system for the study of lineage specification and as an unlimited source of cells for the regenerative ... MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cells and cell lines Figure ... GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes for effective cellular therapies ... culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) of human embryonic stem cells...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells Adult stem cells can be derived ... specific differentiation of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo These cells have the capacity ... of cells into scaffolds 12 1.3.4 Cell source for cell-based tissue engineering for bone regeneration 12 1.4 Stem cells in bone repair 13 1.5 Human Embryonic stem cells...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15 ... 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem ... Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst stage...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... that need to be overcome before clinical application which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem cells (ESCs) are derived ... temporary relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) ... et al., 2008) Human embryonic stem cells (hESCs) are stem cell lines of embryonic origin, isolated from the inner cell mass of the blastocyst-stage embryo (Thomson et al., 1998) Human ESCs have...
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Establishment of autologous culture systems for human embryonic stem cells
... their derivation sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem ... cell source for future clinical applications Literature review Table Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical ... types of cells which are in the closely related families (Sorgner, 2007) Embryonic stem cells Mesenchymal stem cells, Hematopoietic stem cells Oligopotent Can differentiate into only a few cells...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... Figure cells differentiated from human of the vascular Schematic representation of preparationES cellstransplanted Schematic representation of preparation of the transplanted vascular cells differentiated ... suggest that vascular cells derived from human ES cells may have a potential to be a source for therapeutic vascular regeneration after stroke Abbreviations ES cells: Embryonic stem cells; VEGF-R2:...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... NANOFIBERS FOR OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE OF ... Casey K Chan Recovery of Osteogenic Ability of Human Mesenchymal Stem Cells during Serial Passage Using Nanofibrous Scaffolds Proceedings of International Conference on Materials for Advanced Technologies ... Seeram Ramakrishna Mineralized electrospun nanofibrous scaffolds for directing osteogenic differentiation of human mesenchymal stem cells Proceedings of the 241st ACS Meeting and Exposition, 27-31/03/2011,...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... Preparation of conditioned media from !E-MEFs 33! 3.2.3! Human embryonic stem cells and induced pluripotent stem cells 33! 3.2.4! Embryoid body formation 33! 3.2.5! Generation of stable ... conditioned media (CM) used for daily hESC culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery
... use of stem cells 1.2.2 Definition of stem cells 1.2.2.1 Adult stem cells 1.2.2.2 Embryonic stem cells 1.2.2.3 Induced pluripotent stem (iPS) cells ii Chapter II Culture and Propagation of H9 ... possibility of using human embryonic stem cells and derivatives as cellular model in implant testing for two main reasons One, human embryonic stem cells are the very original cells that our human ... of stem cells, adult stem cells (also named as somatic stem cells) , and embryonic stem cells[ 5] However, a third type of pluripotent cell was introduced in 2007 through genetic manipulation of...
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Human embryonic stem cells for genotoxicity testing
... Determination of Genotoxicity using Mitomycin C…………………………… 26 iii CHAPTER 4 RESULTS……………………………………………………… 27 4.1 Characterization of human embryonic stem cells …………………… 27 4.2 Temperature tolerance of human ... established cell lines poorly reflect human physiology Lack of standardization is also a limitation with the use of primary explanted cells Human embryonic stem cells (hESCs) have been validated ... temperature 30 4.23 Chromosomal Analysis of hESC after exposure to low temperature……31 4.3 Evaluation of genotoxic effect of Mitomycin C on human embryonic stem cells using PNA-FISH and mFISH…………………………………………………...
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OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells
... Institute (BTI) and the Agency for Science Technology and Research (A*STAR) Thanks to Prof Wang Nai-Dy, Prof Miranda Yap, Prof Tan Bor Leung, Prof James Chen, and Dr Paul Robson for their guidance, in ... days of humor and inspiration, and providing invaluable thoughts on science and life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse Embryonic Stem Cells ... activity (SDIA) model of neurogenesis, we investigated the effects of the targeted knockdown of Oct-3/4 and Sox-2 by short interfering RNAs (siRNAs) in mouse embryonic stem cells (mESC) Quantitative...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin ... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... both hematopoi- Fig Sequential activation of globin gene transcription during in vitro erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed...
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Atlas of Human Pluripotent Stem Cells doc
... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The ... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...
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báo cáo hóa học:" Human embryonic stem cells hemangioblast express HLA-antigens" pot
... characterization of blast cells Figure of Kinetics of hemangioblast formation in EB culture and characterization of blast cells A) Kinetics of blast colony (BCs) formation from cells derived from EBs ... Generation of functional hemangioblasts from human embryonic stem cells Nat Methods 2007, 4(6):501-9 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation of human embryonic stem ... Platt JL: Immunosuppression by embryonic stem cells Stem Cells 2008, 26(1):89-98 Fabricius D, Bonde S, Zavazava N: Induction of stable mixed chimerism by embryonic stem cells requires functional Fas/...
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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
... Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells Trends Biotechnol 2004, ... limitation of adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells ... approaches in human clinical trails that employ adult stem cells (such as blood-forming hematopoietic stem cells and cartilage-forming cells) A potential advantage of using adult stem cells is that...
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