salt tolerance in transgenic plants
Báo cáo khoa học: Overexpression of putative topoisomerase 6 genes from rice confers stress tolerance in transgenic Arabidopsis plants ppt
... ERD10, KIN1, andKIN2, analyzed in this study, under stress conditions.The differentially expressed genes in transgenic plants that encode proteins that probably function in stress tolerance include ... stress tolerance in transgenic Arabidopsis plants and may be used toengineer stress tolerance in crop plants. Furthermore,for a better understanding of the functions of TOP6genes, transgenic ... homologs.The amino-acid sequence analysis of rice TOP6 pro-teins also predicted several potential putative phos-phorylation sites for casein kinase II, protein kinase C,tyrosine kinase, histidine kinase,...
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Báo cáo Y học: ER–resident chaperone interactions with recombinant antibodies in transgenic plants potx
... ofrecombinant immunoglobulin chains in plants is significant in demonstrating the suitability and potential versatility of transgenic plants for producing a variety of mammalianproteins. Protein translocation ... IgG-expressing plants. Again this is consistent with the model forBiP in assisting protein folding and increasing throughput ofthe ER. In mammals, BiP associates with both Ig light andheavy chains ... binding transiently in the ER to Ig chainsduring protein folding and processing. The immunoblotresults matched those found by Northern blot, in that mostBiP protein was immunoprecipitated in...
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Tài liệu Phytohormones and Abiotic Stress Tolerance in Plants ppt
... Furthermore, minimalbinding domain of BRI1 was determined using binding assays and recombinantBRI1 fragments (Kinoshita et al. 2005). In other plant species, it was also shownthat mutations in BRI1 ... et al. 2002) were determined as positive regulators in ABA signaling.Gene encoding ABA-induced protein kinase 1 (PKABA1), which is a serine–threonine type protein kinase, was isolated from ... receiverdomain and a long C-terminal part containing a single-repeat MYB-type DNA-binding domain (Sakai et al. 1998) called a GARP domain (Riechmann et al. 2000)and the proline- and glutamine-rich...
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Báo cáo Y học: A complex fruit-specific type-2 ribosome-inactivating protein from elderberry (Sambucus nigra) is correctly processed and assembled in transgenic tobacco plants doc
... vulgare) by rubbing thevirus suspension in 100 mMphosphate buffer (pH 7.2)containing 2% poly(vinylpyrrolidone) in the presence ofCarborundum powder. Inoculated plants were maintained in a greenhouse ... cDNAencoding SNA; RIP, ribosome-inactivating protein; SNA, Sambucusnigra agglutinin; SNLRP, Sambucus nigra lectin-related protein;TMV, tobacco mosaic virus.Enzyme: ribosome-inactivating protein ... arelocated in domain 1, and two others (linking Cys147-Cys162 and Cys188-Cys205, respectively) are located in domain 2. The sugar-binding activity of SNA-If relies ontwo carbohydrate-binding sites...
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Tài liệu Báo cáo khoa học: A DExD⁄ H box RNA helicase is important for K+ deprivation responses and tolerance in Arabidopsis thaliana docx
... and in ux systems are very impor-tant for cellular ion relationships in natural conditions.Increasing in ux, decreasing efflux or both can maxi-mize K+uptake to maintain K+homeostasis in plants [32,33]. ... GUS activities in the transgenic seedlings ordifferent tissues was determined after the transgenic plants had been incubated overnight at 37 °Cin1mgÆmL)15-bromo-4-chloro-3-indolyl-glucuronic ... Histochem-ical staining indicated that AtHELPS is mainly expressed in the youngseedlings and vascular tissues of leaves and roots. Using both helps mutantsand overexpression lines, we observed that, in the...
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Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx
... con-taining DNA spots (5 lg each) corresponding to inter-nal fragments of ADC or cruzipain genes. The latter(used as a control) is a housekeeping gene that encodesthe main cysteine proteinase ... to a-amanitin of the transcription of argininedecarboxylase (ADC) and cruzipain genes in transformed Trypano-soma cruzi. Permeable parasites were preincubated at 0 °C for5 min in the absence ... bands obtained in the corresponding Southern blot assay (Fig. 3B) couldonly be explained by the integration, into the parasitegenome, of ‡ 2 units of the plasmid pADC-8 arranged in a head-to-tail...
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Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
... RaddingCM (1998) Interaction of human rad51 recombinationprotein with single-stranded DNA binding protein,RPA. Nucleic Acids Res 26, 5388–5393.17 Stauffer ME & Chazin WJ (2004) Physical interactionbetween ... clonedinto the intermediate vector. Constructs were introducedinto Agrobacterium tumefaciens strain EHA106, and trans-formed into wild-type (Columbia) plants by floral in ltra-tion [35,36]. The transgenic ... soluble in the presence of 6 murea. Soluble fractions were loaded on to columns contain-ing 10 mL His-Bind resin (Novagen). The columns werewashed with 50 mL binding buffer containing 6 m...
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optimisation of artemia biomass production in salt ponds in vietnam and use as feed ingredient in local aquaculture
... processes in food processing procedures (drying, smoking, curing etc.). Toldrá and Flores (1998) suggested that lipolysis is one of the main processes of lipid degradation in fresh meat during processing; ... FFA displayed a threefold increase during drying, suggesting that lipolysis occurs before or during drying, whereas polar lipids and TAG decreased in the dried reindeer meat and were significantly ... critical suggestions in the design of the experimental solar drier, and Dr. Vu Quang Thanh for instruction in indoor drying techniques and allowing me to utilize the drying machines and equipment,...
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TRANSGENIC PLANTS docx
... that its engineering is relatively easy. 24 Transgenic Plants in opine synthesis and in the synthesis of auxin and cytokinin are in the T-DNA, while genes involved in host range and ... This book is printed on acid-free paper. Printed in Singapore by Uto-Print The Concept: Integration and Expression of Alien Genes in Transgenic Plants 11 tumefaciens stains. Several ... entering into maize nuclei. Indeed recent comparison between T-DNA integration in tobacco and in inaize suggested that the relative difficulty in maize transformation involves T-DNA integration...
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Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
... scFv intrabodiesand assessed their binding activity to the WASP-EVH1domain in the scFv gene-transfected T cells. Finally,we succeeded in expressing the functional scFv intra-bodies in the ... reagentsfor Western blotting, immunostaining, immunoprecipi-tation and blocking of protein function. Therefore, ifintrabodies retain their specificity and high-affinity bind-ing properties, they ... R & Oppermann H (1988) Protein engi-neering of antibody binding sites: recovery of specificactivity in an anti-digoxin single-chain Fv analogue pro-duced in Escherichia coli. Proc Natl Acad...
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Biochemical mechanisms of detoxification in higher plants
... contaminants. Dioxins are also formed in high-temperature chemical processes (in- cluding garbage incineration) in which organic and inorganic compounds with one or more atoms of chlorine (including molecular ... processes in metallurgy, metal working, engineering, chemical, chemico-pharmaceutical, petrochemical and other industries; and the ex-haust gases of internal combustion engines using lead-containing ... vitally important parameters determining the continuing existence of mankind. The inexorable and seemingly permanent increase of technogenic contaminants in all ecological niches creates danger...
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Báo cáo khoa học: Expression of CYP2E1 increases oxidative stress and induces apoptosis of cardiomyocytes in transgenic mice pdf
... quantitation of microgram quantities of proteinutilizing the principle of protein-dye binding. AnalBiochem 72, 248–254.CYP2E1 causes apoptosis of cardiomyocytes in vivo W. Zhang et al.1492 FEBS Journal ... longitudinal sections are shown in (A) (magnifi-cation ·20; scale bars = 1 mm). The hemotoxylin and eosin-stained sections of the left ventricle are shown in (B) and Masson’s trichromestained sections ... was increased in CYP2E1 transgenic mice and cTnTR141W transgenic mice (Fig. 6), suggesting that CYP2E1 increases theapoptosis of myocytes through the mitochondrial-dependent pathway. Clinical...
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Báo cáo khoa học: Production of a recombinant mouse monoclonal antibody in transgenic silkworm cocoons pptx
... therefore used in the subsequentexperiments aiming to investigate the increases in L- and H-chain in the cocoons.The proteins contained in the cocoons of the L ⁄ H-and L ⁄ H ⁄ IE1-lines were separated ... enhanced recombinant protein expression in the MSG cells at the level of translation, leading toa 1.5-fold increase in recombinant protein synthesis. In the present study, we generated germline trans-genic ... than that in the H-cocoons. Quantita-tive analysis of the H-chain mRNA in the MSG cellsrevealed that the increase in H-chain in the L⁄ H-linecocoons was not the result of a rise in the mRNA...
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Báo cáo khoa học: Stoichiometry of LHCI antenna polypeptides and characterization of gap and linker pigments in higher plants Photosystem I doc
... stacking and running gel. Ur ea(6M) was also incorporated into the running gel. Thestaining for the densitometry was obtained with 0.05%Coomassie R in 25% isopropanol, 10% acetic acid in orderto ... protein complexes and not free in themembrane (not shown).These r esults suggest t hat t he co-ordination o f Chl mightbe in part d ifferent in P SI and in PSII; PSI binds Chls bothwithin individual ... each Lhca bandboth in PSI–LHCI c omplex and in recombinant samples isreported in Table 1, expressed as IOD (integrated opticaldensity). The dens itometry allows ob taining a better repro-ducibility...
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- salt tolerance in plants
- engineering salt tolerance in plants
- salt tolerance genes in plants
- salt tolerance in tomato plants
- salt tolerance in plants and its effects
- salt tolerance in plants experiment
- salt tolerance in plants ppt
- salt tolerance in plants review
- drought and salt tolerance in plants critical reviews in plant sciences
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