... Furthermore, minimalbinding domain of BRI1 was determined using binding assays and recombinantBRI1 fragments (Kinoshita et al. 2005). In other plant species, it was also shownthat mutations in BRI1 ... several of the CRF genes were down-regulated in response to salt stress. It was suggested that these genes may play animportant role in mediating the input of cytokinin into the salt stress responsepathway ... et al. 2002) were determined as positive regulators in ABA signaling.Gene encoding ABA-induced protein kinase 1 (PKABA1), which is a serine–threonine type protein kinase, was isolated from...
... expressed in plants. Heavy-chain derivatives are shown in gray and light-chainderivatives in pink.Solidblack lines indicate continuation of the polypeptide backbone (simplelines indicate the ... tomato include the high biomass yields (about 68,000 kg/ha, approaching theyields possible in tobacco) and the increased containment offered by growth in greenhouses.Instead of introducing transgenes ... in gray and light chains in pink.Solidblack linesindicate continuation of the polypeptide backbone (simple lines indicate the constant parts of theantibody, curly lines indicate the variable...
... othersbeing delphinidin, peonidin, pelargonidin, petunidin, and malvidin. Of the flavonols,quercetin, kaempferol, myricetin, and isorhamnetin are common in fruits, quercetinbeing the predominant ... organisms.The methods for increasing, preventing, or redirecting flux into or within the path-way include increasing levels of a rate-limiting biosynthetic enzyme, inhibitionof the activity ... third predominant flavonoid group in fruits isproanthocyanidins and their monomer units, catechins (procyanidin) or gallocate-chins (prodelphinidins).Delphinidin-derived anthocyanins are known...
... gene regulonsand regulatory pathways involved in freezing tolerance in Arabidopsis. Freezing tolerance increases in many plants in response to low, non-freezing temperature, aprocess known as ... thought by providingjust such an interdisciplinary forum, in which researchresults, including novel concepts such as environmen-tally induced increases in mutation rates in bacteriaand a ... plants carrying a camta1 ⁄ 3 double mutationare impaired in freezing tolerance [52]. These resultsestablish a role for CAMTA proteins in cold acclima-tion and provide a possible point of integrating...
... ofParkin, PINK1, DJ-1 and HtrA2 activity remains tobe determined.Parkin can be phosphorylated by a number of kinasesincluding casein kinase 1, protein kinase A, proteinkinase C [86] and cyclin-dependant ... six genes have been implicated in thepathogenesis of PD, a-synuclein, Parkin, PTEN-induced putative kinase 1 (PINK1), DJ-1, leucine-richrepeat kinase 2 (LRRK2) and ATP13A2. Mutations in the genes ... genes encoding a-synuclein, LRRK2 and ATP13A2cause autosomal-dominant forms of parkinsonism.Mutations in the genes encoding Parkin, DJ-1 andPINK1 all cause autosomal-recessive parkinsonism ofearly...
... kinins by cytokinin oxidases in plants. Plant Growth Reg.32, 315–327.9. Morris, R.O., Bilyeu, K.D., Laskey, J.G. & Cheikh, N. (1999)Isolation of gene enconding a glycosylated cytokinin ... modeling of the flavoprotein catabolizingplant hormones cytokinins. In Recent Research Develop ments in Proteins, vol. 2. (Pandalai, S .G., ed.), pp. 63–81. TransworldResearch Network, Kerala, India.15.Werner,T.,Motyka,V.,Strnad,M.&Schmu¨lling, ... gene indicates afunction for cytokinins in paracrine signaling in whole tobacco plants. Plant J. 12, 401–415.30. Emanuelsson, O., Nielsen, H., Brunak, S. & von Heijne, G. (2000)Predicting...
... lgÆmL)1proteinase K in PBS-T for 30 s to 35 min depending on the stage. Theywere postfixed in 4% paraformaldehyde and rinsed for5 · 5 min in PBS-T. Prehybridization was performed in hybridization ... and larvae were incubated in preab-sorbed sheep antidigoxigenin-AP Fab fragments at1 : 4000 dilution in blocking solution for 3 h. They wererinsed in PBS-T (6 · 15 min) and then in chromogenicbuffer ... PrP family. Amino acid numberingstarts from the initiator methionine. Gaps inserted to optimize alignments are indicated by dashes. Numbers in parentheses in the align-ments indicate the length...
... due to long range couplinginvolving vicinal isoprenoid moieties. Due to the linebroadening, the precision of signal integration is substan-tially reduced. However, within the experimental limits, ... thetwo competing deprotonation paths is illustrated in Fig. 8 forthe predominant ES complex A of a sample of IPP carrying a13C label in its methyl group; a similar scheme involving aless stable ... known precursor of the vitamins thiamine [11] andpyridoxol [12], could be incorporated into terpenoids byE. coli [9] as well as by higher plants [7]. More specifically, plants were shown to utilize...
... variance in 141 the measured concentration inplants is explained by the variance of the 142 concentrations in soil. 143 In case of an insignificant correlation, the concentration in plant ... uptake of organic contaminants from soil by plants. The goal was to gain insight into both experimental data and predictive methods. Knowledge of uptake of con-taminants inplants is relevant for ... SPME sam-pling kinetics were studied for a number of sample types in orderto (1) determine the sampling time needed to ensure equilibriumsampling, (2) select the best mode of sampling (static...
... forma-tion by introducing the oxygen between carbon 9 and 10of oleic acid before incorporation of the monomer intothe cutin. In this context, it is interesting to note thatinhibition studies ... characterizedenzymes involved in lipid metabolism. The most simi-larly expressed genes are CYP86A8 encoding a fatty acidx-hydroxylase, a putative epoxide hydrolase, several genes encoding enzymes involved in ... represents a highlydiversified set of heme-containing proteins found in bacteria, fungi, animals and plants [7]. In animals,members of the CYP4A gene subfamily mainly cata-lyze the formation of x- and...
... converted toviolaxanthin. In plants, carotenoids are mainlyinvolved in photosynthesis as accessory pigments, in photoprotection (quenching and xanthophylls cycle)and in the formation of abscisic ... Psy2 genesin the codingsequence region. The two genes were shown to have ahighly conserved structure, with seven exons beingalmost identical and six introns being much more vari-able in sequence ... [23] in grass PSYs, differ-ences in the N-terminus as well as the C-terminus ofthe two proteins may result in differences in theirplastid localization.Transcription analysis of the two genes...
... alsoincluded in each assay.Fig. 6. Plasma cortisol levels in the three Angora genotypes(n = 10 per group) following intravenous insulin injection. Plasmaglucose levels are shown in the inset. ... only in the genes encoding CYP17, but also in copy number. Furthermore, in vivoassays revealed that the identified genotypes differed in their ability toproduce cortisol in response to intravenous ... an increase in plasma cortisol levels in response to insulin injection (Fig. 6). However,although the decrease in plasma glucose was similarfor all groups, the amplitude of the response in...
... cadaverine and 1-pip-erideine, because there is no template informationdescribing the target reaction in the current system.For EC 4.1.1.18, the list of high-scoring genes doesnot contain any genes ... Protein- lysine erythro-5- Hydroxylysine Protein- N6-Me-lysine Glutaryl- CoA Glutarate Glutarate semialdehyde 5-Amino pentanoate 1-Piperideine 5-Amino- pentanamide Cadaverine N2-(D-1-Carboxy- ... ethyl)-L-lysine L-2-Aminoadipate 6-semialdehyde L-2-Amino- adipate Saccharopine 6-Amino-2-oxohexanoate6-Acetoamido-2-oxohexanoateD-Lysine2,5-Diamino-hexanoate3,5-Diamino-hexanoateL-β-LysineL-LysineN6-Hydroxy-lysineN6-Acetyl-N6-hydroxy-lysineAerobactin2-Amino-5-oxohexanoate5-Amino-3-oxohexanoateN...
... F (2002) Insights intobinding of fatty acids by fatty acid binding proteins.Mol Cell Biochem 239, 45–54.31 Bo¨rchers T & Spener F (1993) Involvement of arginine in the binding of heme ... mam-malian FABP1 during zebrafish embryogenesis. Thedistinct patterns of expression for the fabp1b (in theintestine) and fabp10 (in the liver) genesin the devel-oping zebrafish combined represent ... acid-binding protein from bovine liver. Mol Cell Biochem123, 23–27.32 Thumser AE, Evans C, Worrall AF & Wilton DC(1994) Effect on ligand binding of arginine mutations in recombinant rat...
... a distinct fragmin-like actin-bindingprotein that controls in vivo phosphorylation of actin bythe actin-fragmin kinase. Eur J Biochem 265, 240–250.37 Donze O & Picard D (2002) RNA interference ... NKA48 contain only a single domain fold.The stability of these two proteins is highly dependenton calcium binding [25]. The typical domain motivescontain a ‘tyrosine corner’ in the domain centre ... function.bc-Crystallins are two-domain proteins found in vertebrate eye lenses and have distant relatives in microorganisms (e.g. the proteins in Fig. 2). Thebc-crystallin domain of spherulin 3a from...