preosteoblasts bone marrow derived mscs adipose derived mscs
Differentiation of bone marrow derived mesenchymal stem cells (BM MSCs) using engineered nanofiber substrates
... cells used in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived mesenchymal ... mesenchymal stem cells (BM -MSCs) 61 2.3.3 Potential of adipose -derived stem cells 63 2.3.4 MSCs derived from other sources 64 2.4 Cell-material constructs as bone grafts ... vessel (BV), lymphoid aggregates (LYM), bone marrow (BM) Fig 6.6: Histology of new bone formation (a) and (b) bone consists of mature bone (pink) and immature bone (cartilage) [H&E], (c) chondrocytes...
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báo cáo hóa học:" Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells" doc
... engineering applications Bone marrow stromal cells (MSCs) are multipotent stem cells originating from the bone marrow stroma, and represent a particularly promising cell source for bone tissue engineering ... [5,6] In contrast, bone marrow- derived endothelial progenitor cells (EPCs) possess high potential for neovascularization and reendothelialization [7] EPCs isolated from bone marrow or peripheral ... viability and osteogenic functions of bone marrow- derived osteoblasts remains to be elucidated Although ECs-initiating vascularization in the engineered bone is critical [15], the survival and...
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báo cáo hóa học: " Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy" potx
... and force feature of immature and mature DCs Materials and methods Preparation of bone marrow cells Bone marrow- derived dendritic cells were generated according to Lutz’s publication [10] with ... cultured at 37.0°C in an incubator containing 5% CO2 to induce differentiation of bone marrow cells into bone marrow- derived dendritic cells Then, the cells were fed once at the interval of day with ... marrow was washed with PBS through a syringe Clusters within the marrow suspension were disintegrated by vigorous pipetting The bone marrow cell suspension was centrifuged at 300 × g for The cells...
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Báo cáo y học: "Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells" ppsx
... expressed in bone marrow- derived or synovium -derived cells Genes whose mRNA expression is differentially expressed in bone marrow- derived or synovium -derived cells Gene Upregulated in bone marrow- derived ... immunocytochemistry, whereas it was negative Figure derived adherent cells Immunosuppressive properties of bone marrow- derived and synoviumderived adherent cells (a) Proliferative activity of ... http://arthritis-research.com/content/7/6/R1304 Figure derived adherent cells In-vitro-induced differentiation of bone marrow- derived and synoviumderived adherent cells (a) Chondrogenic differentiation...
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Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx
... and bone marrow- derived MSCs Bone marrow- derived MSCs have been identified as a potential new therapeutic option to induce therapeutic angiogenesis The main advantage of using bone marrow- derived ... of bone marrow- derived MSCs Flow cytometric analyses of bone marrow- derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 Figure Effect of simvastatin and bone ... of simvastatin on the cell viability of bone marrowderived MSCs in vitro To examine whether simvastatin has anti-apoptotic effect on bone marrow- derived MSCs under hypoxia stress, cells viability...
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Báo cáo y học: "Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus" pot
... (b), immature mDCs (c), mature FLDCs (d), and mature mDCs (e) BMDCs, bone marrow- derived dendritic cells; CD3- BMC, CD3- bone marrow cells; FLDC, dendritic cells induced by FL; FL, FMS-like tyrosine ... independent experiments of seven SLE patients and eight normal controls BMDCs, bone marrow- derived dendritic cells; BMCs, bone marrow cells; FLDCs, dendritic cells induced by FL; mDCs, myeloid dendritic ... Gershwin ME: Expansion of bone marrow IFN-alpha-producing dendritic cells in New Zealand Black (NZB) mice: high level expression of TLR9 and secretion of IFNalpha in NZB bone marrow J Immunol 2004,...
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Báo cáo y học: "Effects of bone marrow-derived cells on monocrotaline- and hypoxia-induced pulmonary hypertension in mice" pps
... Panels A5and B: Quantification of bone marrow- derived cells (BMDCs) engraftment in recipient mouse lung by real-time quanPanels A and B: Quantification of bone marrow- derived cells (BMDCs) engraftment ... multi-lineage engraftment by a single bone marrow- derived stem cell Cell 2001, 105:369-377 Kotton DN, Ma BY, Cardoso WV, Sanderson EA, Summer RS, Williams MC, Fine A: Bone marrow- derived cells as progenitors ... mouse sample for right venFigure the recipient) number means marrow- derived cells recipient), Effect of the number of bone marrow- derived cells on the increases in right ventricular systolic...
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Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps
... http://respiratory-research.com/content/8/1/50 Figure Rat bone marrow- derived EPC in culture Rat bone marrow- derived EPC in culture A highly purified population of EPC was isolated from hindlimb bone marrow of male Sprague-Dawley ... PE CD133 PE HMVEC-L KDR PE CD106 FITC Figure Characteristics of rat bone marrow- derived EPC Characteristics of rat bone marrow- derived EPC Utilizing phase contrast microscopy as well EPC (Figure ... http://respiratory-research.com/content/8/1/50 Figure Proliferative characteristics of bone marrow- derived EPC Proliferative characteristics of bone marrow- derived EPC EPC and rPAEC were cultured in the presence of 20%...
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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... Phan SH: Bone marrowderived progenitor cells in pulmonary fibrosis J Clin Invest 2004, 113(2):243-252 Kotton DN, Ma BY, Cardoso WV, Sanderson EA, Summer RS, Williams MC, Fine A: Bone marrow- derived ... in bone marrow during streptococcal pneumonia Am J Physiol 1996, 271(4 Pt 1):L587-92 21 22 23 24 25 26 27 28 Sato Y, van Eeden SF, English D, Hogg JC: Bacteremic pneumococcal pneumonia: bone marrow ... http://respiratory-research.com/content/9/1/25 Figure Frequency of labeled donor cells in bone marrow Frequency of labeled donor cells in bone marrow BM cells were harvested from recipients which were sacrificed...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 10
... waste chondrocytes, Bone Marrow derived Mesenchymal Stem Cells (BM MSCs) , or any combination of these cells (29, 214, 215, 267, 268) Various authors have suggested the use of BM MSCs for cell-based ... potential than BM MSCs, SMSC have not been used clinically In addition, the process of harvesting SMSCs requires the use of arthroscopy, which is more invasive than bone marrow biopsy for BM MSCs The ... for example with stimulating the target site to attract more MSCs (with releasing more signals) 114 Chapter Autologous Bone Marrow Derived Mesenchymal Stem Cell versus Autologous Chondrocyte...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 1
... for cartilage repair such as bone marrow derived stem cells (BM MSCs) , adipose derived stem cells (ADSCs), periostal cells, skeletal muscle and synovial fibroblasts derived cells (63-68), 12 ... stem cells Bone marrow, peripheral blood, cord blood All mature blood cell components Mesenchymal stem cells Bone marrow, peripheral blood Bone, Cartilage, tendon, adipose tissue, muscle, marrow ... Multipotent stem cells especially the ones from bone marrow and adipose tissue are very promising solution for cartilage repair Bone marrow- derived stem cells (BM MSCs) can be easily obtained and differentiated...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 2
... that SPIO were indeed in the cytoplasm of the labeled MSCs (figure 3.5) 60 Figure 3-5 Transmission electron microscopy of the labeled MSCs TEM confirmed the presence of the ferucarbotran ... site to ensure that all the cartilage was resected, the joint was closed and rested for one week MSCs (107 cells) labeled with 50µg/ml ferucarbotran (10pg/cell) were seeded in ml hyaluronan and ... group) and MSC seeded hyaluronan (experimental group) 57 3.4 Results 3.4.1 Characterization of MSCs Cells cultured from aspirated BM had characteristic of fibroblastic spindleshape morphology...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 3
... assay MTS assay showed that labeling of the MSCs did not affect the proliferation rate of the cells over time 3.4.6 Differentiation potential of labeled MSC MSCs are multipotent stem cells, which ... staining showed that MSCs labeled with SPIO did not affect osteogenic differentiation capacity of cells (figure 3.10) Figure 3-10 Alizarin Red staining Alizarin Red staining of the MSCs was used ... developing fat vacuoles (Figure 3.9) 64 Figure 3-9 Oil red O staining Oil red O staining of the MSCs after 21 days adipogenic differentiation induction; Unlabeled (A), labeled with 25 µg/ml ferucarbotran...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 4
... Post injection MR images MR images of the mini-pig knee joint immediately after the 107 labeled MSCs injection in the knee capsule FSE PD sequence (A) provided more anatomical data and 3D-GRE ... cell localization data (Arrowheads show the distribution of the labeled cells in the knee joint) MSCs were distributed in different places of the knee (figure 3.14) The labeled cells migrated to ... after the cells injection Upper panel (right knee) show the knee injected with HA plus labeled MSCs, however lower panel (left knee) shows the HA only injection (control knee) (Arrowheads indicate...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 6
... controlled labeling concentration of MSCs can reduce the adverse effects of the iron particles Our results showed, after optimized labeling, that visualization of injected MSCs is indeed feasible in vivo ... contrast, our study showed that iron labeling did not affect the adipogenic and osteogenic potential of MSCs in vitro but could negatively affect viability and chondrogenic differentiation of the labeled ... SPIO labeling method of the cells can be a promising tool for real time evaluation and tracking of MSCs, providing data for a better understanding of the migration and localization of the injected...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 7
... the treatment of damaged cartilage Bone marrow (BM) derived mesenchymal stem cell (MSC) is a good candidate because of their multipotent nature The use of MSCs for cartilage repair relies on ... cartilage tissue, injured cartilage tissue and MSCs Then, migration of MSCs against the injured cartilage tissue was evaluated Exploring the interaction of MSCs with injured cartilage tissue can open ... were examined by culturing MSCs (passage 3) in complete media and using the supplemented media with platelet derived growth factor (PDGF), a known chemo-attractant for MSCs (235), in the conditioned...
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Proteomic analysis of the response of murine bone marrow derived macrophages to IFN y stimulation and infection with staphylococcus aureus
... Introduction 1.5 A Dissertation reproducible experimental system - Bone marrow derived macrophages in serum-free culture Murine bone marrow derived macrophages (BMMs) are a good model for studying macrophage ... of mouse stem cells and their differentiation to bone- marrow derived macrophages (BMMs) was conducted as described by Eske [2] Briefly, bone marrow cells from tibias and femurs of n=3 or n=15 ... BMMC57BL/6 70 Discussion 79 4.1 2-DE proteome reference map of bone marrow derived macrophages 79 4.2 IFN-γ effects on BMM-BALB/c and BMM-C57BL/6 identified by proteomic...
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Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
... and bones of TNF-transgenic mice stromal cellsstromal cell -derived factor-1 expression in bone marDecreased stromal cell -derived factor-1 expression in bone marrow row stromal cells and bones ... els and increases blood bone marrow stromal cell -derived TNF injection decreases osteoclast precursor frequency factor-1 levinjection decreases bone marrow stromal cell -derived factor1 levels ... (Figure 7) Discussion boneinjectiondecreases stromal cell -derived factor expression by TNF injection stromal cellsstromal cell -derived factor expression marrow decreases by bone marrow stromal cells...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... GFP-labeled MSCs were observed in the lungs, liver, bone marrow and normal femoral head, and the amount of GFP positive http://www.medsci.org Int J Med Sci 2011, MSCs in the bone marrow was higher ... intravenous transplantation of MSCs Whether intravenous transplantation of MSCs can cause immunological rejection? Studies on the immunogenicity of bone marrow MSCs reveal that MSCs can not only avoid ... migration of MSCs after transplantation A: immediately after intravenous MSCs transplantation; B: h after MSCs transplantation; C: 24 h after MSCs transplantation; D: 48 h after MSCs transplantation;...
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