isolation culture and osteogenic chondrogenic differentiation of bone marrow derived mesenchymal stem cells
báo cáo hóa học:" Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels" docx
Ngày tải lên :
18/06/2014, 15:20
... Characterization of the cell types and growth kinetics of cultured cells Healthy growing EC and SMC cultures were obtained from UC of group-1 and group-2 individuals No contamination of SMCs in EC cultures ... weight and growth characteristics of cultured cells No differences in terms of average time to reach passage cell density, percentage of viable cells and senescence level were found for each cell culture ... indirect immunofluorescence and total number of cells was visualized under differential interference contrast (DIC) Representative merged micrographs of immunofluorescence and DIC images of exponentially...
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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
Ngày tải lên :
07/08/2014, 18:21
... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...
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Báo cáo y học: " Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates " pps
Ngày tải lên :
12/08/2014, 13:22
... bone marrow- derived mesenchymal stem cells in mice Am J Physiol Lung Cell Mol Physiol 2007, 293:L131-141 Rojas M, Xu J, Woods CR, Mora AL, Spears W, Roman J, Brigham KL: Bone marrow- derived mesenchymal ... Popov B, Lee JW, Serikov V, Matthay MA: Intrapulmonary delivery of bone marrow- derived mesenchymal stem cells improves survival and attenuates endotoxin-induced acute lung injury in mice J Immunol ... mechanism of action of MSCs, and KGF and angiopoietin-1 are Figure Bacterial Growth in vivo (A and B) and in vitro (C) Bacterial colony forming units in BAL fluid (A) and blood (B) at 1, 3, and days...
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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot
Ngày tải lên :
07/08/2014, 23:22
... ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu erew ,gk 95.0 ± 77.3 dehgiew ... wef a ,revewoh ;noitamrof ytivac fo egatnecrep tsellams eht detartsnomed puorg PC eht ,noitidda nI spuorg GBCU dna CSM-BCU eht ni noitamrof ytivac fo egatnecrep rellams a dnuof ew ,sllec mets detnalpsnart ... sllec detnalpsnart eht rof troppus reffo yam droc derujni eht ,droc lanips eht fo sdne lamixorp dna latsid eht ot noitcennoc rof ,slairetam ro sllec suoirav fo pu edam dloffacs ro egdirb a htiw...
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Báo cáo khoa học: "Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs" ppsx
Ngày tải lên :
07/08/2014, 23:22
... of mixture of β-TCP and cells revealed significantly greater area of new bone formation than control (p ＜ 0.05) and a smaller amount of residual TCP Previous studies showed that bone marrow- derived ... human bone marrow and umbilical cord blood Stem Cells 2006, 24, 679-685 Chen L, Tredget EE, Wu PYG, Wu Y Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells ... SP Culture- expanded, bone marrow- derived mesenchymal stem cells can regenerate a critical-sized segmental bone defect Tissue Eng 1997, 3, 173-185 13 Kadiyala S, Young RG, Thiede MA, Bruder SP Culture...
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Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps
Ngày tải lên :
07/08/2014, 23:22
... Anghileri E, Andreini A, Pizzolo G, Sbarbati A, Bonetti B Induction of neural-like differentiation in human mesenchymal stem cells derived from bone marrow, fat, spleen and thymus Bone 2007, 40, ... in the bottom of the tube (Fig 3D), and positive to toluidine blue staining (Figs 4E and F) Discussion Isolation and characterization of stem cells derived from various tissues and sources have ... 15 passages [34] Fig Osteogenic and chondrogenic differentiation of cUCB-MSCs A-C: Osteogenic differentiation A and B: Alizarin red S staining A: Undifferentiation (UDF), B: Differentiation (DF),...
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Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx
Ngày tải lên :
09/08/2014, 10:23
... concentration and annulus cells adiposederived mesenchymal stem of AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and ... interactions of adult mesenchymal stem cells and disc cells by Le Visage et al [20] showed that annulus, but not nucleus, cells co-cultured in chondrogenic pellets with mesenchymal stem cells had ... CD, cluster of differentiation plastic adherence and lineage specific differentiation satisfy the standard criteria suggested for defining mesenchymal stem cells Mesenchymal stem cells should...
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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx
Ngày tải lên :
10/08/2014, 10:20
... of mesenchymal stem cells derived from bone marrow and adipose tissue J Cell Biochem 2006, 99:1285-1297 Kern S, Eichler H, Stoeve J, Kluter H, Bieback K: Comparative analysis of mesenchymal stem ... endothelial cells enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of ... human marrow- derived mesenchymal stem cells Exp Hematol 2006, 34:1604-1605 Ng AM, Kojima K, Kodoma S, Ruszymah BH, Aminuddin BS, Vacanti AC: Isolation techniques of murine bone marrow progenitor cells...
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Báo cáo y học: "HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells" pot
Ngày tải lên :
13/08/2014, 01:20
... smooth muscle and endothelial cells To study the effects of HIV-1 on the differentiation of these cells, the interaction of HIV-1 and recombinant gp120 on MSC differentiation to adipogenic and endothelial ... infection of human bone marrow stromal fibroblasts Blood 1990, 76:317-322 40 Wang L, Mondal D, La Russa VF, Agrawal KC: Suppression of clonogenic potential of human bone marrow mesenchymal stem cells ... USA) and employed between passages and To induce adipogenic differentiation, confluent cells were cultured as follows: three cycles of days induction medium and days maintenance medium of hMSC Mesenchymal...
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Báo cáo sinh học: "Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model" potx
Ngày tải lên :
18/06/2014, 22:20
... percentages of all EPC surface markers were similar between day and day 14 of cell culture Additionally, the percentages of MSC surface markers of CD45+ and CD271+ cells did not differ between day and ... Prevention of endotoxin-induced systemic response by bone marrow- derived mesenchymal stem cells in mice Am J Physiol Lung Cell Mol Physiol 2007, 293:L131-141 23 Salgar S, Manning E, Ruiz P, Pham S: Mesenchymal ... Therapeutic potential of mesenchymal stem cells for severe acute lung injury Chest 2010, 138:965-972 20 Chistiakov DA: Endogenous and exogenous stem cells: a role in lung repair and use in airway...
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Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx
Ngày tải lên :
10/08/2014, 05:21
... Page of 10 Figure Characterization of bone marrow- derived MSCs Flow cytometric analyses of bone marrow- derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 ... with NIH image software Effect of simvastatin on the cell viability of bone marrowderived MSCs in vitro To examine whether simvastatin has anti-apoptotic effect on bone marrow- derived MSCs under ... Effect of simvastatin on the cell viability of bone marrow -derived MSCs in vitro In vitro, serum starvation induced bone marrow- derived MSCs apoptosis, as indicated by flow cytometry and MTT...
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Differentiation of bone marrow derived mesenchymal stem cells (BM MSCs) using engineered nanofiber substrates
Ngày tải lên :
11/09/2015, 09:57
... Types of cells used in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived mesenchymal ... capture of osteoblasts within 30 minutes The osteogenic differentiation potential of bone marrow derived mesenchymal stem cells (BM-MSCs) was achieved by manipulating the physical, biochemical and ... cells: MSCs, osteoprogenitor cells, osteoblasts, chondrocytes Mitogenic for mesenchymal cells and osteoblasts, chemotactic for inflammatory and mesenchymal cells Targeted cells: mesenchymal and...
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Báo cáo sinh học: " Human umbilical cord blood-derived mononuclear cell transplantation: case series of 30 subjects with Hereditary Ataxia'''' docx
Ngày tải lên :
18/06/2014, 19:20
... Journal of Translational Medicine 2011, 9:65 http://www.translational-medicine.com/content/9/1/65 hUCB are mainly comprised of a heterogenous population of hematopoietic and mesenchymal stem cells, ... within an interval of five to seven days Two ml of CSF was removed and replaced by ml of cell suspension during the intrathecal injection In terms of intravenous infusion, 30 ml of cell suspension ... Discussion The frequency of exact diagnosis and confirmation of hereditary ataxias has risen in tandem with advances in genetic testing that define the different types and the locus of genotype variation,...
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báo cáo hóa học:" Human umbilical cord blood-derived mononuclear cell transplantation: case series of 30 subjects with Hereditary Ataxia" pdf
Ngày tải lên :
20/06/2014, 03:20
... Journal of Translational Medicine 2011, 9:65 http://www.translational-medicine.com/content/9/1/65 hUCB are mainly comprised of a heterogenous population of hematopoietic and mesenchymal stem cells, ... within an interval of five to seven days Two ml of CSF was removed and replaced by ml of cell suspension during the intrathecal injection In terms of intravenous infusion, 30 ml of cell suspension ... Discussion The frequency of exact diagnosis and confirmation of hereditary ataxias has risen in tandem with advances in genetic testing that define the different types and the locus of genotype variation,...
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Báo cáo y học: "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions" pdf
Ngày tải lên :
10/08/2014, 05:21
... triggers differentiation of HUMSCs into cardiomyocytes and maturation of HUMSCs derived cardiomyocytes Admittedly, the detailed mechanism(s) of above effects of S1P on differentiation of and maturation ... with proliferation of hESC cultures He et al [10] recently further investigated the role of S1P in the growth and multipotency maintenance of human bone marrow and adipose tissue -derived MSCs They ... with Regard to the Source of Fetus -Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem cells from whole human umbilical...
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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps
Ngày tải lên :
12/08/2014, 15:22
... Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow Stem Cells 2007, 25:1384-1392 Weiss ML, Anderson ... Age-related changes in human bone marrow- derived mesenchymal stem cells: consequences for cell therapies Mech Ageing Dev 2008, 129:163-173 Rao MS, Mattson MP: Stem cells and aging: expanding the possibilities ... treatment of inflammatory arthritis [11] Mesenchymal stem cells (MSCs) are cells of stromal origin that can exert profound immunosuppression by modulating T and B cell proliferation and differentiation, ...
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Immunological characterization of human umbilical cord lining derived cells and their therapeutic application in a diabetic mouse model
Ngày tải lên :
11/09/2015, 10:02
... embryonic, fetal/neonatal and adult I will review first embryonic stem cells, induced pluripotent stem cells and then the adult bone marrow derived stem cells, which are well known and best studied for ... their stemness but poses potential risk of iatrogenic oncogenesis 2.3 Bone marrow derived mesenchymal stem cells Adult stem cells, also known as somatic stem cells, are found throughout body after ... Review……………………………………………… 2.1 Embryonic stem cells ………………………………… ……………………….9 2.2 Induced pluripotent stem cells ……………………………………………… 13 2.3 Bone marrow derived mesenchymal stem cells ……………………………….14 2.3.1...
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Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
Ngày tải lên :
19/02/2014, 02:20
... banding patterns and different glycoforms of PrPC In this study, we have analyzed the glycoform patterns of PrPC of human, sheep, cattle and mice and compared them Variable immunoreactivity of ... analysis and diagrammatic presentation of PrPC bands obtained from three different regions of sheep brains (A) Immunodetection of PrPC derived from cortex (c), cerebellum (cb) and brain stem (bs) of ... SAF34 and 8G8 for humans, SAF34, 8G8 and P4 for sheep, SAF34 and P4 for cattle, and SAF34 for mice; and for the C-terminal antibodies 6H4, SAF60 and SAF70 for humans, and mAbs 6H4, SAF60, SAF70 and...
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