pneumophila infection of bone marrow derived macrophages
Proteomic analysis of the response of murine bone marrow derived macrophages to IFN y stimulation and infection with staphylococcus aureus
Ngày tải lên :
12/05/2016, 21:57
... stimulated macrophages respond to internalized S aureus 13 Introduction 1.5 A Dissertation reproducible experimental system - Bone marrow derived macrophages in serum-free culture Murine bone marrow derived ... cultivation of mouse stem cells and their differentiation to bone- marrow derived macrophages (BMMs) was conducted as described by Eske [2] Briefly, bone marrow cells from tibias and femurs of n=3 ... and secretion of pro-inflammatory cytokines However, information about the changes in the proteome of macrophages upon activation by IFN-γ is still limited Murine bone marrow derived macrophages...
- 143
- 213
- 0
Báo cáo y học: "Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus" pot
Ngày tải lên :
12/08/2014, 12:20
... Gershwin ME: Expansion of bone marrow IFN-alpha-producing dendritic cells in New Zealand Black (NZB) mice: high level expression of TLR9 and secretion of IFNalpha in NZB bone marrow J Immunol 2004, ... did those of normal controls *P < 0.05; **P < 0.01 Results are represented as mean ± SD of independent experiments of seven SLE patients and eight normal controls BMDCs, bone marrow- derived dendritic ... photographs of freshly isolated CD3- BMCs (a) and May-Grunwald-Giemsa-stained cytospin preparations of immature FLDCs (b), immature mDCs (c), mature FLDCs (d), and mature mDCs (e) BMDCs, bone marrow- derived...
- 12
- 244
- 0
Báo cáo y học: "Effects of bone marrow-derived cells on monocrotaline- and hypoxia-induced pulmonary hypertension in mice" pps
Ngày tải lên :
12/08/2014, 15:20
... by a single bone marrow- derived stem cell Cell 2001, 105:369-377 Kotton DN, Ma BY, Cardoso WV, Sanderson EA, Summer RS, Williams MC, Fine A: Bone marrow- derived cells as progenitors of lung alveolar ... Detection of GFP-positive bone marrow- derived cells in lungs from recipient mice Immunohistochemical analysis of lung sections from control eGFP-positive mice revealed strong immunostain- Page of 13 ... suggests that bone marrow- derived cells (BMDCs) are able to colonize the adult lung following injury In mice subjected to total body irradiation, transplantation of a single donor marrow- derived stem...
- 13
- 306
- 0
Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
Ngày tải lên :
12/08/2014, 15:21
... Number of QD-labeled donor cells in BM = Fraction of donor cells in BM × total number of BMCs The number of BMCs harvested from femurs and tibias was considered to represent 18.1% of total murine marrow ... http://respiratory-research.com/content/9/1/25 Figure Frequency of labeled donor cells in bone marrow Frequency of labeled donor cells in bone marrow BM cells were harvested from recipients which were ... frequency of labeled cells was measured by flow cytometry and the total number of labeled donor cells in recipients' bone marrow was calculated The results are shown as percentages of total number of...
- 10
- 257
- 0
Differentiation of bone marrow derived mesenchymal stem cells (BM MSCs) using engineered nanofiber substrates
Ngày tải lên :
11/09/2015, 09:57
... Types of cells used in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived ... vessel (BV), lymphoid aggregates (LYM), bone marrow (BM) Fig 6.6: Histology of new bone formation (a) and (b) bone consists of mature bone (pink) and immature bone (cartilage) [H&E], (c) chondrocytes ... than that of the diameter of the cells [10] One of the key components of bone tissue engineering is the concept of ECM ECM not only provides the structural and functional aspects of bone, it also...
- 241
- 446
- 0
báo cáo hóa học:" Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells" doc
Ngày tải lên :
20/06/2014, 01:20
... in biomaterials of bone graft is frequently neglected Therefore, the objective of this study was to evaluate the biocompatibility of the HA-PCL biomaterials to both bone marrow- derived osteogenic ... of PCLHA scaffolds with bone marrow- derived endothelial cells, Page of (page number not for citation purposes) Journal of Orthopaedic Surgery and Research 2009, 4:5 Figure Characterizations of ... functions of bone marrow- derived osteoblasts remains to be elucidated Although ECs-initiating vascularization in the engineered bone is critical [15], the survival and bioactivity of EPCs-originated...
- 9
- 337
- 2
báo cáo hóa học: " Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy" potx
Ngày tải lên :
21/06/2014, 02:20
... and force feature of immature and mature DCs Materials and methods Preparation of bone marrow cells Bone marrow- derived dendritic cells were generated according to Lutz’s publication [10] with ... 109/L Induction and separation of bone marrow- derived dendritic cells The above cells were seeded into a 6-well plate to the end volume of mL per well, and 10.0 μg/L of rmGM-CSF (PeproTech, Rocky ... μg/L of rmIL4 (PeproTech) was added to the corresponding wells in the plate and cultured at 37.0°C in an incubator containing 5% CO2 to induce differentiation of bone marrow cells into bone marrow- derived...
- 9
- 469
- 0
Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx
Ngày tải lên :
10/08/2014, 05:21
... Journal of Biomedical Science 2010, 17:75 http://www.jbiomedsci.com/content/17/1/75 Page of 10 Figure Characterization of bone marrow- derived MSCs Flow cytometric analyses of bone marrow- derived ... with NIH image software Effect of simvastatin on the cell viability of bone marrowderived MSCs in vitro To examine whether simvastatin has anti-apoptotic effect on bone marrow- derived MSCs under ... combination group (p < 0.05) Effect of simvastatin on the cell viability of bone marrow -derived MSCs in vitro In vitro, serum starvation induced bone marrow- derived MSCs apoptosis, as indicated...
- 10
- 230
- 0
Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps
Ngày tải lên :
12/08/2014, 15:20
... by rat bone marrow- derived EPC Capillary-like structures formed by rat bone marrow- derived EPC Formation of tubular structures on Matrigel by EPC as a form of organisation characteristic of EC ... P1H12 PE CD133 PE HMVEC-L KDR PE CD106 FITC Figure Characteristics of rat bone marrow- derived EPC Characteristics of rat bone marrow- derived EPC Utilizing phase contrast microscopy as well EPC (Figure ... Results Characteristics of ex vivo-generated bone marrow- derived rat EPC Cells were harvested from the non-adherent fraction of rat bone marrow mononuclear cells after 48 h of culture on fibronectin-coated...
- 17
- 241
- 0
báo cáo hóa học:" A practical approach for the validation of sterility, endotoxin and potency testing of bone marrow mononucleated cells used in cardiac regeneration in compliance with good manufacturing practice" docx
Ngày tải lên :
18/06/2014, 15:20
... for bone marrow derived mononucleated cells Migration Migration and invasion assay results for bone marrow derived mononucleated cells Page of (page number not for citation purposes) Journal of ... density gradient centrifugation (bone marrow selected cells) (n = 4) Phenotypical analysis of whole bone marrow cells and after density gradient centrifugation (bone marrow selected cells) (n = 4) ... (SWISS-AMI, NCT00355186) A total of 50 ml of bone marrow was aspirated into heparin-treated syringes from the posterior iliac crest under local anesthesia Bone marrow was filtered by using a 100...
- 9
- 773
- 0
Báo cáo y học: "Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells" ppsx
Ngày tải lên :
09/08/2014, 07:20
... expressed in bone marrow- derived or synovium -derived cells Genes whose mRNA expression is differentially expressed in bone marrow- derived or synovium -derived cells Gene Upregulated in bone marrow- derived ... differentiation profile of MSC account for the increase of bone density and loss of cartilage that are characteristics of OA Recent data suggest a possible involvement of MSC in the pathophysiology of OA, ... whereas it was negative Figure derived adherent cells Immunosuppressive properties of bone marrow- derived and synoviumderived adherent cells (a) Proliferative activity of T cells in a mixed lymphocyte...
- 12
- 342
- 0
báo cáo khoa học: "Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging" ppsx
Ngày tải lên :
11/08/2014, 00:22
... Cultivation of Rat/Mouse Mesenchymal Cells from Bone Marrow To obtain bone marrow cells, tibias and femurs were isolated, the epiphyses were removed, the bones were individually inserted in mL automatic ... after 1, 7, 14 and 21 days of culture (D) Comparison of the number of MSCs labeled with Feridex after 21 days of culture in the groups illustrated in (A-C) The percentage of labeled cells was significantly ... Representative images demonstrating labeling of MSCs with Feridex in the absence or presence of agents facilitating uptake of the nanoparticles (A-F”) Presence of Feridex in MSCs was detected by Prussian...
- 13
- 256
- 0
Báo cáo khoa hoc:" Effects of bone marrow cell transplant on thyroid function in an I131-induced low T4 and elevated TSH rat model" docx
Ngày tải lên :
11/08/2014, 08:20
... number of copies of the Y chromosome, also presented a partial restore of the normal histological architecture of the thyroid Conclusion Both a significant increase of TSH and decrease of T4 can ... outside the bone marrow Then both ends of each femur were cut and the bone marrow was washed out with phosphate-buffered saline (PBS) Cells were then cultured using DMEM F12 Page of (page number ... transplantation of stem cells TSH and T4 concentrations weeks after transplantation of stem cells TSH and T4 serum levels of all groups weeks after BM derived cells transplantation All concentrations of T4...
- 8
- 270
- 0
Báo cáo y học: "Development of bone marrow lesions is associated with adverse effects on knee cartilage while resolution is associated with improvement - a potential target for prevention of knee osteoarthritis: a longitudinal study" pdf
Ngày tải lên :
12/08/2014, 11:22
... train length of 10, with a slice thickness of 3.0 mm, a 1.0 inter slice gap, excitation, a field of view of 13 cm, and a matrix of 256 × 192 pixels was also obtained [8] Assessment of BMLs BMLs ... bottom and loss of thickness of less than 50%; grade 3, deep ulceration with loss of thickness of more than 50%; grade 4, full-thickness cartilage wear with exposure of subchondral bone A cartilage ... Correlation of the development of knee pain with enlarging bone marrow lesions on magnetic resonance imaging Arthritis Rheum 2007, 56:2986-2992 Zanetti M, Bruder E, Romero J, Hodler J: Bone marrow...
- 8
- 372
- 0
Effects of combined mechanical and pulsed electromagnetic field stimulations on the osteogenesis of bone marrow stem cells
Ngày tải lên :
09/09/2015, 17:52
... Composition of Bone From a macro perspective, there are two types of bone structure, the compact (cortical) bone and the trabecular (cancellous) bone Cortical bone forms the dense outer shell of most bones ... 80% of the total bone mass Cortical bone is mostly found in the shaft portion of long bones and the outer shell found around the spongy bone at the end of joints It is denser than cancellous bone ... woven bone Remodeling phase – Replacement of woven bone by lamellar bone and the resorption of excess callus Gradual modification of the fracture region leads to restoration of normal bone architecture...
- 209
- 1.8K
- 0
Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 10
Ngày tải lên :
10/09/2015, 09:01
... milliliters of each patient’s blood were collected as well The bone marrow aspirate was processed within 60 minutes The heparinized bone marrow aspirate was mixed with a one-fifth volume of 6% (w/v) ... integration of repaired cartilage to the adjacent cartilage and subchondral bone, bone marrow edema, and lesion 137 osteophytes, the lack of MRIs had minimal effect on interpretation of results ... outcome of MSCs in the injured cartilage was as good as chondrocytes (as a golden standard) The use of MSCs offers some advantages over chondrocytes such as reduced degree of invasiveness (bone marrow...
- 59
- 329
- 0
Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 1
Ngày tải lên :
10/09/2015, 09:10
... Design of microfluidic device 87 4.3.2 Computational modeling of concentration gradient 88 4.3.3 Fabrication of microfluidic device 89 4.3.4 MSC characterization and culture in microfluidic ... stem cells Bone marrow, peripheral blood, cord blood All mature blood cell components Mesenchymal stem cells Bone marrow, peripheral blood Bone, Cartilage, tendon, adipose tissue, muscle, marrow ... co-supervisor Professor Shih-Chang Wang, Head of Discipline of Medical Imaging at Sydney Medical School, University of Sydney, for his constant support and encouragement My gratitude is also towards Professor...
- 70
- 355
- 0
Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 2
Ngày tải lên :
10/09/2015, 09:10
... mixed with different amount of ferucarbotran (used as a representative of different concentrations of the labeled cells, assuming pg Fe per cell) 3.3.13 MR imaging of live animals The pig was ... maximum 14 Total smooth area of the reparative cartilage compared with the entire area of the cartilage defect Average thickness of the reparative cartilage compared with that of the surrounding cartilage ... chondral defect of mm in diameter and 1-2 mm in depth (C) was created in weight-bearing of each medial femoral condyle 3.3.12 Preliminary MR imaging experiments To optimize the imaging of mini-pig...
- 14
- 304
- 0
Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 3
Ngày tải lên :
10/09/2015, 09:11
... the agarose mixed with different amount of Ferucarbotran, which can be a representative of different concentrations of the labeled cells (the average amount of the iron nanoparticles per cell was ... osteogenic differentiation capacity of cells (figure 3.10) Figure 3-10 Alizarin Red staining Alizarin Red staining of the MSCs was used to visualize the calcium deposition of the cells after 21 days ... distribution of collagen type II in extracellular matrix of cells was same between unlabeled and 25 and 50 µg/mL SPIO labeling, however the matrix production were decreased in labeling concentration of...
- 7
- 218
- 0