marrow derived stem cells in hepatology

Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx

Ngày tải lên : 07/08/2014, 23:22

... New canine spinal cord injury model free from laminectomy Brain Res Brain Res Protoc 2005, 14, 171-180 11 Gimble J, Guilak F Adipose -derived adult stem cells: Transplantation of ASCs in spinal ... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using ... B: Oil red O stained after weeks incubation at adipogenic media C: ASCs cultured in control media, not stained with Alizarin red S D: Intense Alizarin red S stained after weeks incubation at osteogenic...

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Báo cáo y học: "Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus" pot

Ngày tải lên : 12/08/2014, 12:20

... migration of dendritic cells in intestinal lamina propria to mesenteric lymph nodes J Immunol 2006, 176:803-810 43 Ronnblom L, Alm GV: The natural interferon-alpha producing cells in systemic lupus erythematosus ... dendritic cells induced by FL; mDCs, myeloid dendritic cells induced by GM-CSF + IL-4; FL, FMS-like tyrosine kinase ligand; GM-CSF, granulocyte macrophage-colony-stimulating factor; IL-4, interleukin ... further important insight into the role(s) of pDCs in SLE pathogenesis We hypothesize that pDCs are the dominant DCs during their development in the BM These IFN-α-producing cells induce the development...

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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot

Ngày tải lên : 07/08/2014, 23:22

... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C ... latipsoH gnihcaeT lacideM yranireteV ytisrevinU lanoitaN luoeS ta god lergnom gk 85 a fo noitces nairaseaC eht gnirud deniatbo saw BCU :noitcelloc lleC sCSM-BCU eninac fo snoitaraperP dehsilbatse saw ... yliad semit eerht tsael ta demrofrep saw noisserpxe reddalb launam ,dedeen fi dna ,tinu erac evisnetni na ni derotinom erew sgod eht ,noitarepo eht retfA h 21 retfa devomer neht dna ,erutus epyt...

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Báo cáo y học: "Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2" potx

Ngày tải lên : 09/08/2014, 10:23

... reported during in vitro chodrogenesis in mesenchymal stem cells [48] FGF2 is routinely used in the culture of bone marrow- derived mesenchymal stem cells, and we have determined a baseline for the ... vasculature 3G5 (a, b) staining predominantly the tunica adventitia consisting of supporting tissue in the vasculature, vWF (c, d) staining predominantly the tunica intima consisting of the endothelial ... particular benefit in increasing the production of matrix in cartilage tissue-engineered in vitro The inhibition of actin stress fibres by FGF-2 in adult human chondrocytes results in an upregulation...

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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx

Ngày tải lên : 10/08/2014, 23:21

... brought into an operating room and placed in a supine position She was then sedated with propofol mg intravenously (push) and a 20 mg/hour rate of continuous infusion After cleaning her abdominal ... 10:2 CaCl2) In order to inject the mixture of stem cells and PRP, our patient was first placed in a lateral position with her left side down After cleaning with povodine-iodine and draping with ... maceration After obtaining ADSCs and preparing PRP as described above for the first two patients, she was prepared for injection of the mixture into the joint In order to inject the stem cell and PRP...

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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Ngày tải lên : 13/08/2014, 23:20

... funding of research involving the more than 60 existing stem cell lines, but will not sanction or encourage destruction of additional human embryos Indeed, the embryos from which the existing ... Fischbach GD, Fischbach RL: Stem cells: science, policy, and ethics J Clin Invest 2004, 114:1364-1370 Donovan PJ, Gearhart J: The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 ... Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human...

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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

Ngày tải lên : 25/10/2012, 11:18

... DXS small animal imaging system is a real-time imaging system which can be used to observe the distribution of cells in living animals in a real time pattern In the imaging system, GFP fluorescence ... expressed in injured tissues play important roles (2) Stem cells circulate among tissues, and stem cells migrate to the injured tissues once injury occurs Stem cell homing is a complicated process in ... are a lot of labeling methods including GFP labeling, Lacz labeling, BrdU labeling, Y chromosome labeling and DiI labeling GFP protein is stable GFP gene can be transfected into MSCs through...

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Stem Cells in Human Reproduction Basic Science and Therapeutic Potential Second Edition pot

Ngày tải lên : 22/03/2014, 20:21

... embryonic stem cells into ovarian structures containing oocytes Stem Cells 2006; 24:266–273 24 Qing T, Shi Y, Qin H, et al Induction of oocyte-like cells from mouse embryonic stem cells by co-culture ... polyadenylated during meiotic maturation and binds CPE-binding protein (CPEB), a highly conserved RNA-binding protein with both a zinc finger and an RNA-recognition motif (RRM) (53,54) Injection into Xenopus ... stored mRNAs RINGO/Spy protein is absent in the oocyte cytoplasm, while its mRNA is maintained in a dormant state This is achieved by binding of Pumilio-2, an RNA-binding protein initially described...

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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx

Ngày tải lên : 18/06/2014, 16:20

... were required to ingest two capsules of Stem- Kine in the morning and two in the evening for 14 days Phenotypic assessment of circulating stem cells Peripheral blood mononuclear cells (PBMC) were ... These data suggest Stem- Kine supplementation increases circulation of cells capable of giving rise to hematopoieticlineage cells in vitro Stem- Kine augments circulation of cells with EPC phenotype ... postbone marrow injury conditions [43], subsequent studies have expanded this finding to situations of inflammation [44], and peripheral tissue injury [45-47] Hematopoietic stem cells are being increasingly...

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báo cáo hóa học:" Elevated adipogenesis of marrow mesenchymal stem cells during early steroid-associated osteonecrosis development" pptx

Ngày tải lên : 20/06/2014, 00:20

... neutral buffered formalin followed by incubating with 60% propylene glycol, then incubated with a newly filtered Oil Red O staining solution After staining, the cells were rinsed with distilled ... 8-isoprostane increases scavenger receptor A and matrix metalloproteinase activity in THP-1 macrophages, resulting in long-lived foam cells Eur J Clin Invest 2004, 34:451-458 Zhang G, Qin L, Sheng ... observation and the increase in lipid deposition remains unexplained One possibility is that marrow lipid was a consequence of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results...

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báo cáo hóa học: " Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy" potx

Ngày tải lên : 21/06/2014, 02:20

... corresponding wells in the plate and cultured at 37.0°C in an incubator containing 5% CO2 to induce differentiation of bone marrow cells into bone marrow- derived dendritic cells Then, the cells were ... protein kinase (p38 MAPK), ERK1/2, phosphoinositide 3-OH kinase (PI3 kinase)/Akt, and NFkappaB pathways in the process of DC maturation PI3 kinase/Akt signaling pathways are important in maintaining ... is going to be contaminated at the first touch and continue with the following touches, and this contamination can influence the next interaction of the tip with the cells Therefore, contamination...

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Báo cáo y học: "Mesenchymal stem cells in arthritic disease" pot

Ngày tải lên : 09/08/2014, 13:22

... delivered via either adenoviral infection or osmotic pumps in inhibiting bleomycin-induced increases in TNF-α, IL-1α, and trafficking of lymphocytes and neutrophils into the lung [56] These successful ... bone marrow- derived mesenchymal cells At the onset of CIA in a mouse model of RA, before inflammation, primitive bone marrow stromal cells migrated from the bone marrow into the affected joint ... produce inflammatory mediators, including IL-1, TNF-α, IFN-γ, and IL-6, and secrete MMPs, leading to the systemic inflammation that eventually results in joint destruction [78,80] Pharmacological interventions...

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Báo cáo y học: "Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study" pptx

Ngày tải lên : 10/08/2014, 09:22

... errors in trypsin specificity Carbamidomethylation of cysteines was taken as fixed modifications, and methionine oxidation and formylation of lysine, arginine and N-terminal amino acids as variable ... search settings used, we identified significant proteins as follows: LCNEC contained a total of 1,124 proteins including 410 unique, 168 in the overlap only between LCNEC and SCLC, 93 in the overlap ... protein, the greater its expression level in LCNEC compared with SCLC and vice versa Representative proteins up-regulated in LCNEC were AL1A1, AK1C1, AK1C3, brain-type fatty acid-binding protein...

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báo cáo khoa học: " Stem cells in clinical practice: applications and warnings" pot

Ngày tải lên : 10/08/2014, 10:20

... lineages such as intestine [52] and from the ectodermal tissues including skin [53], deciduous teeth [54] and nerve tissue [8,9,55,56] ASCs originate during ontogenesis and remain in a marginal ... uncertainties must be borne in mind during the independent peer review of the preclinical data Only when the compelling preclinical data are available, careful and incremental testing in patients ... islets and reintroduce physiological secretion of human insulin AHSCT improves b -cells function and frequently decreases the exogenous insulin need [20] or induces a persistent insulin independence...

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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc

Ngày tải lên : 10/08/2014, 10:21

... mesenchymal stem cells to conditioned medium from tumor cells or bone marrow cells Stem Cells 2007, 25:520-528 Reya T, Morrison SJ, Clarke MF, et al: Stem cells, cancer, and cancer stem cells Nature ... signaling target genes:cyclin D, PCNA, survivin, b-catenin Methods Ninety albino female rats inbred strain (Cux1: HEL1) of matched age and weight (6 months-1 year & 120-150 gm) were included in ... tissues, including the liver Microvesicles are mediators of cell reprogramming Following injury, tissues release chemokines attracting circulating BMDC, and can produce microvesicles including RNA,...

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