4 zebrafish embryonic stem cells

OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells

... B E1 4 P3 AB 2.2 –P AB 35 –T ET AB A– –T P4 ET B– E1 4 P4 TE E1 TA 4 – P4 TE TB TE –P T-R 42 +V EC TR L E1 AB – P3 2 AB – – P3 AB TET A– – P4 E1 TET 4 B – T E1 P – ET A 41 – TE PC TB P41 DN ... 1 345 4. 55 2.98 3.68 3 .49 4. 36 19 .4 33.9 28.2 36.7 34. 3 Fgf -4 Utf-1 Utf-1 Fbx-15 Fbx-15 Opn Opn 552 - 633 382 - 644 865 - 1063 2 14 - 305 866 - 951 600 - 682 880 - 969 3.3 Poor Amplification 4. 07 ... 0.60% 0.70% Utf-1 12 hr s 24 hr s 0% Opn 5% 10% 15% 20% 25% 30% 35% 40 % 45 % 0.50% 1.00% 1.50% 2.00% 2.50% 3.00% 3.50% 4. 00% 4. 50% 12 hr s 24 hr s 0.00% Fgf -4 12 hr s * * 24 hr s * * 0.00% 0.05% 0.10%...

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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"

Danh mục: Y học thưởng thức

... control maintained at 37oC 2. 34 ± 0.09 - 4oC for 24h 1.51 ± 0.10 64. 4 ± 4. 4 % 25oC for 24h 1.81 ± 0.11 77.3 ± 4. 8 % 4oC for 48 h 1.62 ± 0.06 69.0 ± 2.3 % 25oC for 48 h 1.66 ± 0.01 71.0 ± 0.5 % ... 25oC and 4oC for 24h was 77.3 ± 4. 8 % and 64. 4 ± 4. 4 % respectively (significantly different, P < 0.05) The corresponding survival rates Int J Med Sci 2006, after 48 h exposure to 25oC and 4oC was ... Biotechnol 2000;18 (4) :399 -40 4 Richards M, Fong CY, Tan S, Chan WK, Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 20 04; 22(5):779-89...

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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Danh mục: Báo cáo khoa học

... 1009–1018 34 Szutorisz H, Canzonetta C, Georgiou A, Chow CM, Tora L & Dillon N (2005) Formation of an active Globin locus activation during differentiation 35 36 37 38 39 40 41 42 43 44 tissue-specific ... genes are not induced during in vitro differentiation of scl-null embryonic stem cells Blood 90, 143 5– 144 7 FEBS Journal 273 (2006) 746 –755 ª 2006 The Authors Journal compilation ª 2006 FEBS 755 ... histone acetylation pattern of a tissue-specific chromatin domain, Proc Natl Acad Sci USA 97, 144 94 144 99 7 54 11 Bottardi S, Aumont A, Grosveld F & Milot E (2003) Developmental stage-specific epigenetic...

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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx

Danh mục: Báo cáo khoa học

... 16 24 1630 ª 2008 The Authors Journal compilation ª 2008 FEBS 1625 Epigenetic study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic ... apparent ED between ES cells and EG cells, the ED of TS cells to ES cells (77) is greater than that of EG cells to ES cells (49 ), confirming the similarity of EG cells to ES cells DNA methylation ... methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing...

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embryonic stem cells, methods and protocols - kursad turksen

Danh mục: Sinh học

... 0-89603-881-5 (alk paper) Embryonic Stem Cells Laboratory manuals I Turksen, Kursad II Series QH 440 .5 E43 2002 612'.0181 dc21 200102 645 9 Preface It is fair to say that embryonic stem (ES) cells have taken ... arrest in embryonic stem cells FEBS Lett 42 5, 49 95 04 11 Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe-Nebenius, D., Chambers, I., et al (1998) Formation of pluripotent stem cells in ... 2.0 ì 1 04 3.0 ì 105 4. 0 ì 105 4. 0 ì 105 6.0 ì 105 2.0 ì 106 1 .4 ì 105 6.0 ì 1 04 1.01.5 ì107 total cells We typically split cultures at ratios from 16 to 18 resulting in approx 1.52.0 ì 106 cells...

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differentiation of embryonic stem cells

Danh mục: Sinh học

... Repopulating Cells from Embryonic Stem Cells The In Vitro Diﬀerentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of Megakaryocytes from Murine Embryonic Stem Cells ... Mouse Embryonic Stem Cells YIJING CHEN, JAY L VIVIAN, AND TERRY MAGNUSON 40 6 Section IV Differentiation of Monkey and Human Embryonic Stem Cells 29 Growth and Diﬀerentiation Monkey ES Cells of ... Mouse Embryonic Germ Cells 25 Isolation and Culture of Embryonic Germ Cells MARIA P DE MIGUEL AND PETER J DONOVAN 353 Section III Gene Discovery by Manipulation of Mouse Embryonic Stem Cells...

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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx

Danh mục: Hóa học - Dầu khí

... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), ... saline(1.372 ± 0. 041 mm2: n = 10) and the hMNC- (1 .43 8 ± 0.0 84 mm2: n = 10) injected groups The infarct area in the hES-EC- (1.308 ± 0.0 94 mm2: n = 6) or the hES-MC(1. 249 ± 0. 047 mm2: n = 6) injected ... Saline F hES-ECs+MCs hES ECs+MCs ss -DNA+ cells (/mm2 ) * 60 50 40 30 20 10 Saline hES ECs+MCs Exercise time on rota-rod (sec) 42 40 38 36 34 32 30 28 26 24 22 20 Saline hMNCs hES- ECs hES- MCs...

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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Danh mục: Hóa học - Dầu khí

... cell pluripotency and early embryonic development by the tran- http://www.translational-medicine.com/content/7/1/20 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 Nat Cell Biol 2006, ... pluripotency of embryonic stem cells Nature 2006, 44 4(7117):3 64- 368 Zhang J, Tam WL, Tong GQ, Wu Q, Chan HY, Soh BS, Lou Y, Yang J, Ma Y, Chai L, et al.: Sall4 modulates embryonic stem cell pluripotency ... of Pou5f1 8(10):11 14- 1123 Zhou Q, Chipperfield H, Melton DA, Wong WH: A gene regulatory network in mouse embryonic stem cells Proc Natl Acad Sci USA 2007, 1 04( 42):1 643 8-1 644 3 Kim J, Chu J, Shen...

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báo cáo hóa học:" Human embryonic stem cells hemangioblast express HLA-antigens" pot

Danh mục: Hóa học - Dầu khí

... minutes at 4 C The antibodies included: CD45-PerCp, CD 34- FITC, CD31-PE (from Becton Dickinson), CD 146 AF 647 , CD 144 (VE-cadherin)-PE, Flt-1-PE (from R&D Systems), CD33-PerCp (eBioscience) The cells were ... immune-privileged properties Stem Cells 20 04, 22 (4) :44 8-56 Grinnemo KH, Kumagai-Braesch M, Mansson-Broberg A, Skottman H, Hao X, Siddiqui A, et al.: Human embryonic stem cells are immunogenic in ... engraftment of mouse embryonic stem cells in allogeneic recipients Stem Cells 2006, 24( 10):2192-201 Magliocca JF, Held IK, Odorico JS: Undifferentiated murine embryonic stem cells cannot induce...

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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES doc

Danh mục: Sức khỏe giới tính

... stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem ... [ 244 ], β-catenin can interact NURF [ 244 ] with Brg1 [ 64] Smad2 interacts with NURF NURF [ 244 ] [ 244 ] Brg1 [237] NURF [ 244 ] Chromatin Remodeling Brg1 [232] Baf155 [236] Baf250b [238] Snf2h [ 241 ] ... pathways in human embryonic stem cells Stem Cells, 2006 24( 6): p 147 6-86 [105] Katoh, M., CER1 is a common target of WNT and NODAL signaling pathways in human embryonic stem cells Int J Mol Med,...

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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES pdf

Danh mục: Sức khỏe giới tính

... stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem ... [ 244 ], β-catenin can interact NURF [ 244 ] with Brg1 [ 64] Smad2 interacts with NURF NURF [ 244 ] [ 244 ] Brg1 [237] NURF [ 244 ] Chromatin Remodeling Brg1 [232] Baf155 [236] Baf250b [238] Snf2h [ 241 ] ... pathways in human embryonic stem cells Stem Cells, 2006 24( 6): p 147 6-86 [105] Katoh, M., CER1 is a common target of WNT and NODAL signaling pathways in human embryonic stem cells Int J Mol Med,...

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EMBRYONIC STEM CELLS – BASIC BIOLOGY TO BIOENGINEERING doc

Danh mục: Sức khỏe giới tính

... 18 46 XX 46 XX 46 XX 46 XX 46 XY 46 XX 46 XY 46 XX Table Unique features of 18 hESC lines (+) represents that HESC line is shown to be positive for those expression markers 26 Embryonic Stem Cells ... ES cells in vitro FASEB J, Vol 14, No 15, (Dec), pp (2 540 -2 548 ) 16 Embryonic Stem Cells – Basic Biology to Bioengineering Murry, C.E., & Keller, G (2008) Differentiation of embryonic stem cells ... 49 no of lines Karyotype 46 XY 46 XX 46 XX 46 XY 46 XX 46 XX 46 XY 46 XX 46 XX OZ Embryo source Embryo grades PGD/PGS results OZ-1 OZ-2 IVF/ICSI IVF/ICSI IVF/ICSI OZ-3 OZ -4 OZ-5 OZ-6 OZ-7 OZ-8 PGS...

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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf

Danh mục: Báo cáo khoa học

... Human placenta feeder layers support undifferentiated growth of primate embryonic stem cells Stem Cells 20 04, 22, 43 3 -44 0 45 Moore K, Piedrahita JA The effects of human leukemia inhibitory factors ... (LIF) Dev Biol 1990, 141 , 344 -352 53 Pera MF, Trounson AO Human embryonic stem cells: prospects for development Development 20 04, 131, 55155525 54 Pesce M, Schöler HR Oct -4: Control of totipotency ... cultured pluripotential cells by retroviral vector Nature 1986, 323, 44 5 -44 8 63 Robertson EJ Teratocarcinomas and embryonic stem cells: a practical approach IRL Press, Oxford, 1987 64 Rose TM, Weiford...

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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf

Danh mục: Báo cáo khoa học

... of pluripotency in mouse embryonic stem cells Cloning Stem Cells 20 04, 6, 386-391 17 Cobb MH, Goldsmith EJ How MAP kinases are regulated J Biol Chem 1995, 270, 148 43- 148 46 18 Cochrane CG Cellular ... dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress 255 10, 42 3 -42 6 12 Brook FA, Gardner RL The origin and efficient derivation of embryonic stem cells in the mouse ... 239- 246 33 Heo JS, Lee YJ, Han HJ EGF stimulates proliferation of mouse embryonic stem cells: involvement of Ca2+ influx and p 44/ 42 MAPKs Am J Physiol Cell Physiol 2006, 290, C123-133 34 Ikeda...

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The signals of FGFs on the neurogenesis of embryonic stem cells ppsx

Danh mục: Báo cáo khoa học

... dopamine neurons from human embryonic stem cells Proc Natl Acad Sci USA 20 04, 101:12 543 -12 548 Ying QL, Stavridis M, Griffiths D, Li M, Smith A: Conversion of embryonic stem cells into neuroectodermal ... mouse embryonic stem cells Dev Biol 1999, 210:30 -43 Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells Dev Growth Differ 2006, 48 :513-523 ... inhibitors in differentiating ES cells (A) FGF1 at 40 ng/ml was applied to 46 C ES cells on individual days (D1, D2, D3, D4) or from day through (D1 -4) (B) Derived GFP+ cells were analyzed by FACS...

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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps

Danh mục: Báo cáo khoa học

... depletion/reoxygenation conditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE- 24) were routinely grown on 0.1% gelatin-coated ... 2005, 44 :8059-8067 doi:10.1186/ 142 3-0127-18 -46 Cite this article as: Jia et al.: Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells ... markers SSEA-1 (Mab4301) and SSEA -4 (Mab43 04, Millipore, Billerica, MA) to ensure no differentiation Only cells within the 20th passage were used Page of 10 5% carbon dioxide at 37°C Cells were removed...

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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

Danh mục: Báo cáo khoa học

... promoter: H3K4me3 Gene body: H3K36me3 General [1 04] ESC, embryonic stem cell; CTCF, CCCTC-binding factor, insulator associated protein; hESC, human embryonic stem cell; iPSC, induced pluripotent stem ... K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, 7:263-269 94 Deng J, Shoemaker R, Xie B, Gore A, LeProust EM, Antosiewicz-Bourget ... incorporation provides a unique and functionally essential telomeric chromatin in embryonic stem cells Genome Res 2009, 19 :40 4 -41 4 103 Ng RK, Gurdon JB: Epigenetic memory of an active gene state depends...

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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt

Danh mục: Báo cáo khoa học

... of osteogenic cells derived from human embryonic stem cells Tissue Eng 20 04, 10:1518-1525 17 Chaudhry GR, Yao D, Smith A, Hussain A: Osteogenic Cells Derived From Embryonic Stem Cells Produced ... directing the differentiation of stem cells into the osteogenic lineage in vitro J Bone Miner Res 20 04, 19:1379-13 94 Page of 38 Trounson A: Human embryonic stem cells: mother of all cell and tissue ... 186:107-1 24 35 Burt RK, Verda L, Kim DA, Oyama Y, Luo K, Link C: Embryonic stem cells as an alternate marrow donor source: engraftment without graft-versushost disease J Exp Med 20 04, 199:895-9 04 36...

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báo cáo khoa học:" Induction of osteogenic markers in differentially treated cultures of embryonic stem cells" ppt

Danh mục: Báo cáo khoa học

... murine embryonic stem cells Biochem Biophys Res Commun 2001, 2 84( 2) :47 8 -48 4 Yamashita A, Takada T, Narita J, Yamamoto G, Torii R: Osteoblastic differentiation of monkey embryonic stem cells in ... pluripotency in mouse embryonic stem cells Cloning Stem Cells 20 04, 6 (4) :386-391 Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D: Inhibition of pluripotential embryonic stem cell differentiation ... which was Figure stem cells (ESCs) Mineral deposition at day 14 in differently treated embryonic Mineral deposition at day 14 in differently treated embryonic stem cells (ESCs) Cells were exposed...

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