derivation methods for human embryonic stem cells past present and future pdf
Establishment of autologous culture systems for human embryonic stem cells
... (Sorgner, 2007) Embryonic stem cells Mesenchymal stem cells, Hematopoietic stem cells Oligopotent Can differentiate into only a few cells (Sorgner, 2007) Lymphoid stem cells, Myeloid stem cells Unipotent ... embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff Bongso, 2005, see Table 2) Each type of stem cells holds its ... cell source for future clinical applications Literature review Table Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... but for the support system my family has been Thank you, Appa and Mummy for all the love and laughter and for inspiring me to follow my heart Sumitha, Aji and Tamanna, and Susan, thank you for ... 1.1 Embryonic stem cells 1.2 Gastrulation– formation of mesoderm and endoderm in the embryo .8 1.3 Regenerative medicine and embryonic stem cells 10 1.3.1 Diabetes– a candidate ... GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes for effective cellular...
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Báo cáo khoa học: "An overview of oak silviculture in the United States: the past, present, and future" pdf
... central and eastern United States forest and form the upland and bottomland oak ecosystems Together, these 114 million and provide a habitat for > 75 tree species, 230 species of birds and mammals, ... rangelands of California, Oregon, and Washington and from the northern mixed conifer forests of the Great Lakes and Canada southward to the bottomland hardwood and southern yellow pine woodlands ... Ozarks US For Serv Res Pap NC 251 Schnur GL (1937) Yield, stand, and volume tables for even-aged upland oak forests US For Serv Tech Bull 560 SR (1987) A generalized system of models forecasting...
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báo cáo khoa học: " Genome Medicine: past, present and future" pdf
... Public Health Genomics, Centers for Disease Control and Prevention, 1600 Clifton Rd, NE, MS E61, Atlanta, GA 30333, USA 4Departments of Molecular and Human Genetics and Pediatrics, Baylor College ... applications of formalin-fixed paraffin-embedded tissues Clin Lab Med 2009, 29:101-113 Page of doi:10.1186/gm220 Cite this article as: Auffray C, et al.: Genome Medicine: past, present and future Genome ... scientific methods to document how such technologies can improve health and prevent disease in practice Dealing with the genomics evidence gap will require two key and interrelated science and policy...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee ... engineering, and the challenges that need to be overcome before clinical application which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem ... hESCs for cartilage regeneration and tissue engineering 2.2.1 Expansion of hESCs Human embryonic stem cells are conventionally co-cultured on mouse embryonic fibroblast (MEF) feeder cells for their...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and ... AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... Rada-Iglesias A, Wysocka J: Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease Genome Medicine 2011, 3:36 ...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of pluripotent stem ... adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have also been isolated from several other organs such as the brain (neuronal stem ... reports suggest that adult stem cells such as haemopoietic stem cells, neuronal stem cells and mesenchymal stem cells could cross boundaries and differentiate into cells of a different tissue...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery
... literature review and comparison for all three types of stem cells, adult stem cells, embryonic stem cells and induced pluripotent stem cells, embryonic stem cells are inevitable and irreplaceable ... possibility of using human embryonic stem cells and derivatives as cellular model in implant testing for two main reasons One, human embryonic stem cells are the very original cells that our human body ... Biocompatibility 1.2 Stem cells ………………………………………………………………….4 1.2.1 Significance in the use of stem cells 1.2.2 Definition of stem cells 1.2.2.1 Adult stem cells 1.2.2.2 Embryonic stem cells 1.2.2.3 Induced...
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Human embryonic stem cells for genotoxicity testing
... RESULTS……………………………………………………… 27 4.1 Characterization of human embryonic stem cells …………………… 27 4.2 Temperature tolerance of human embryonic stem cells …………… 30 4.21 Survival rate of hESC after exposure ... reflect human physiology Lack of standardization is also a limitation with the use of primary explanted cells Human embryonic stem cells (hESCs) have been validated as a permanently stable and healthy ... OBJECTIVES……………………………………………………… CHAPTER 2 LITERATURE REVIEW…………………………………………….4 2.1 Human Embryonic Stem Cells (hESCs): Derivation, Isolation and Maintenance of hESCs…………………………………………………………………………………… 2.2 ESCs Characterization…………………………………………………………….6...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... B and C colonies were 94.5%, 3.0% and 2.5% respectively for 24h exposure to 4oC (n=200); and 97.5%, 1.0% and 1.5% respectively for 24h exposure to 25oC (n=200) The proportion of Grade A, B and ... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts:...
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Guidelines for Human Embryonic Stem Cell Research potx
... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell ... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html Scientific Background of Human Embryonic Stem Cell Research INTRODUCTION Human embryonic stem cells (hES cells) ... materials and might have importance to the donor The characterization of stem cells for purposes of standardization and for validation of results The safe handling and storage of blastocysts and stem...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... (Peprotech) and expanded these cells After five passages in culture (= approximately 30 days after the sorting), we obtained the expanded cells as a mixture of ECs and MCs derived from human ES cells ... Abbreviations ES cells: Embryonic stem cells; VEGF-R2: vascular endothelial growth factor receptor type 2; ECs: endothelial cells; MCs: mural cells; hMNCs: human peripheral blood mononuclear cells; MCAo: ... VE-cadherin+VEGF-R2+TRA-1- cells on day 10 of differentiation were also positive for CD34, CD31 and eNOS [10] Therefore, we used the term 'eEC' for these ECs in the early differentiation stage We sorted and expanded...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... been reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying ... either hES cells or adult cells (Figure 6, panel B) For miR-106b, miR-92, miR-93, miR-130a and miR-190, the difference in their expression between EB and hES cells and between EB and adult cells were ... Background Human embryonic stem (hES) cells possess unique features: self-renewal and pluripotency They can be continuously cultured in undifferentiated status and give rise to differentiated cells and...
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báo cáo hóa học:" Human embryonic stem cells hemangioblast express HLA-antigens" pot
... poly-Llysine (Sigma) and dried for 24 hours H9 cells, EB (day 3) cells and BC (day 6) cells were harvested, washed in PBS, and were allowed to settle on the coated coverslips for 30 at 37°C The cells were ... molecules on murine embryonic stem cells Biol Reprod 1997, 57(3):561-8 Bonde S, Zavazava N: Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients Stem Cells 2006, 24(10):2192-201 ... MHC proteins in human embryonic stem cells Proc Natl Acad Sci USA 2002, 99(15):9864-9 Li L, Baroja ML, Majumdar A, Chadwick K, Rouleau A, Gallacher L, et al.: Human embryonic stem cells possess...
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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
... approaches in human clinical trails that employ adult stem cells (such as blood-forming hematopoietic stem cells and cartilage-forming cells) A potential advantage of using adult stem cells is that ... adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells may ... blastocysts for derivation of human cloned ES cells Therefore, no information or protocols for obtaining human cloned blastocysts were available Absent any report of an efficient protocol for human...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... events underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have ... 3.2.3! Human embryonic stem cells and induced pluripotent stem cells 33! 3.2.4! Embryoid body formation 33! 3.2.5! Generation of stable cell lines 34! 3.2.6! Neurosphere formation ... characterized by forebrain malformation and associated with mental retardation and severe craniofacial anomalies like cyclopia or proboscis formation (Ming and Muenke, 1998) The most severe form is embryonic...
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Baculovirus mediated genetic modification of human embryonic stem cells
... of human embryonic stem cells Although lentiviral vectors are widely used in gene transfer to human embryonic stem cells, there is still a need for vectors and methods to achieve efficient and ... 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell research began with the derivation of hES cell line and ... is, the cells are pluripotent These two properties make human embryonic stem cells promising and attractive for use in regenerative medicine and studies of human development as well as for pharmaceutical...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... allogenic) and from the bone marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells ... hESCs-dervied osteogenic cells in bone formation 21 CHAPTER MATERIAL & METHODS 22 2.1 MATERIAL & METHODS Culture and maintenance of Human Embryonic Stem Cells Human embryonic stem cells (hESCs) used ... culture 1.4 Stem cells in bone repair Stem cells are the fundamental source of tissue for any organism They provide the body with cells that are needed both for growth as well as repair Stem cells...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem ... HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15 ... family Page X Chapter Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass...
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Báo cáo y học: "Regulatory T cells in systemic lupus erythematosus: past, present and future" pot
... Foxp3 since information about T regulatory cells and T helper cells in SLE is very limited Information on invariant natural killer T cells in SLE has recently been reviewed [9] These cells also ... Figure 1) Regulatory T cells and dendritic cells in human SLE In the past it had been generally believed that decreased numbers and/ or function of Tregs contribute to the T-cell and B-cell hyperactivity ... regulatory and effector cells [64] Both naïve and most previously activated CD4+ cells stain brightly for CD127 Although most human CD4+CD25highFoxp3+ Tregs are previously activated memory cells, ...
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