introducing exogenous regulators into embryonic stem cells

báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

Ngày tải lên : 11/08/2014, 12:21

... in pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous ... prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a Proc Natl Acad Sci U S A 2000, 97:5237-5242 72 Altun G, Loring JF, Laurent LC: DNA methylation in embryonic stem cells ... Pelegrini M, Plath K, Lowry WE: Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M,...

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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages

Ngày tải lên : 04/10/2015, 16:03

... T cells 14 Figure 1.5 Haematopoietic development Pluripotent stem cells give rise to multipotent haematopoietic stem cells that differentiate into lymphoid and myeloid progenitors T -cells, B -cells ... μ – Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising ... General Introduction 1.1 Embryonic stem cells 1.2 Gastrulation– formation of mesoderm and endoderm in the embryo .8 1.3 Regenerative medicine and embryonic stem cells 10 1.3.1...

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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"

Ngày tải lên : 31/10/2012, 16:57

... A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... Marshall VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from ... some cells detached after exposure to low temperature Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown) All of the detached cells...

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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Ngày tải lên : 07/03/2014, 12:20

... erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time points ... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the Rex-1 and b-actin genes but not the embryonic and...

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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx

Ngày tải lên : 23/03/2014, 07:20

... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells derived ... methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing ... ED between ES cells and EG cells, the ED of TS cells to ES cells (77) is greater than that of EG cells to ES cells (49), confirming the similarity of EG cells to ES cells DNA methylation profiles...

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embryonic stem cells, methods and protocols - kursad turksen

Ngày tải lên : 08/04/2014, 12:51

... Chapter 33.) Murine Embryonic Stem Cells 1 Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells Marsha L Roach and John D McNeish Introduction Embryonic stem (ES) cells were rst ... suspension into another 15-mL tube (one tube is for freezing and one is to maintain cells) Pellet the cells by centrifugation at 110g for Murine Embryonic Stem Cells 10 While the cells are in ... human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers Mol Med 6, 8895 17 Reubinoff, B., Pera, M., Fong, C., Trounson, A., and Bongso, A (2000) Embryonic stem...

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differentiation of embryonic stem cells

Ngày tải lên : 11/04/2014, 00:32

... Repopulating Cells from Embryonic Stem Cells The In Vitro Diﬀerentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of Megakaryocytes from Murine Embryonic Stem Cells ... Culture of Embryonic Germ Cells MARIA P DE MIGUEL AND PETER J DONOVAN 353 Section III Gene Discovery by Manipulation of Mouse Embryonic Stem Cells 26 Gene Trap Mutagenesis in Embryonic Stem Cells ... 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Diﬀerentiation of Embryonic Stem Cells 13 Gene Targeting Strategies for...

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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx

Ngày tải lên : 18/06/2014, 15:20

... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), ... suggest that vascular cells derived from human ES cells may have a potential to be a source for therapeutic vascular regeneration after stroke Abbreviations ES cells: Embryonic stem cells; VEGF-R2: ... TRA-1(-) cells VEGF-R2(+) / VE-cadherin (-) / TRA-1 (-) cells expansion with VEGF expansion with PDGF - BB VE-cadherin (+) cells VE-cadherin (-) aSMA (+) cells hES -ECs hES -ECs+MCs aSMA (+) cells...

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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Ngày tải lên : 18/06/2014, 15:20

... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that ... mouse embryonic stem cells Proc Natl Acad Sci USA 2007, 104(42):16438-16443 Kim J, Chu J, Shen X, Wang J, Orkin SH: An extended transcriptional network for pluripotency of embryonic stem cells ... supervised clustering (A) Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical...

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báo cáo hóa học:" Human embryonic stem cells hemangioblast express HLA-antigens" pot

Ngày tải lên : 18/06/2014, 15:20

... molecules on murine embryonic stem cells Biol Reprod 1997, 57(3):561-8 Bonde S, Zavazava N: Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients Stem Cells 2006, 24(10):2192-201 ... susceptible to immune rejection than adult cells Stem Cells 2006, 24(2):221-9 Koch CA, Geraldes P, Platt JL: Immunosuppression by embryonic stem cells Stem Cells 2008, 26(1):89-98 Fabricius D, Bonde ... hemangioblasts from human embryonic stem cells Nat Methods 2007, 4(6):501-9 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation of human embryonic stem cells progresses Page...

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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES doc

Ngày tải lên : 27/06/2014, 19:20

... stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem ... pathways in human embryonic stem cells Stem Cells, 2006 24(6): p 1476-86 [105] Katoh, M., CER1 is a common target of WNT and NODAL signaling pathways in human embryonic stem cells Int J Mol Med, ... differentiation of cells derived from human embryonic stem cells Proc Natl Acad Sci U S A, 2000 97(21): p 11307-12 [20] Kroon, E., et al., Pancreatic endoderm derived from human embryonic stem cells generates...

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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES pdf

Ngày tải lên : 28/06/2014, 04:20

... stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem ... pathways in human embryonic stem cells Stem Cells, 2006 24(6): p 1476-86 [105] Katoh, M., CER1 is a common target of WNT and NODAL signaling pathways in human embryonic stem cells Int J Mol Med, ... differentiation of cells derived from human embryonic stem cells Proc Natl Acad Sci U S A, 2000 97(21): p 11307-12 [20] Kroon, E., et al., Pancreatic endoderm derived from human embryonic stem cells generates...

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EMBRYONIC STEM CELLS – BASIC BIOLOGY TO BIOENGINEERING doc

Ngày tải lên : 28/06/2014, 05:20

... Nakatsuji VI Contents Chapter Embryonic Stem Cells: Introducing Exogenous Regulators into Embryonic Stem Cells 123 Yong-Pil Cheon Chapter Functional Control of Target Single Cells in ES Cell Clusters ... Applications of Embryonic Stem Cells in Research and Development 191 Chapter 11 Methods to Generate Chimeric Mice from Embryonic Stem Cells 193 Kun-Hsiung Lee Chapter 12 Embryonic Stem Cells in Toxicological ... assessment of retinal pigment epithelium from human embryonic stem cells using transcriptomics Cloning Stem Cells, Vol 6, No 3, pp (217-245) Embryonic Stem Cells for Therapies – Challenges and Possibilities...

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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf

Ngày tải lên : 07/08/2014, 18:21

... and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells Trends Biotechnol 2004, ... from mouse embryonic stem cells Nat Biotechnol 2000, 18, 675-679 41 Levenberg S, Golub JS, Amit M, Itskovitz-Eldor J, Langer R Endothelial cells derived from human embryonic stem cells Proc Natl ... adult cells are already specialized, their potential to regenerate damaged tissue is limited Another limitation of adult stem cells is their inability to effectively grow in Human embryonic stem cells...

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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf

Ngày tải lên : 07/08/2014, 20:23

... 2003, 61 (Suppl 1), 2-7 16 Chambers I The molecular basis of pluripotency in mouse embryonic stem cells Cloning Stem Cells 2004, 6, 386-391 17 Cobb MH, Goldsmith EJ How MAP kinases are regulated J ... H2O2-induced ROS generation in mouse -5 embryonic stem cells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed to H2O2 10 M for a ... on mouse embryonic stem cells exposed to H2O2-induced oxidative stress 253 Fig Effect of dihydrotestosterone (DHT) on hydrogen peroxide (H2O2)-induced decrease of DNA synthesis A-D: The cells were...

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The signals of FGFs on the neurogenesis of embryonic stem cells ppsx

Ngày tải lên : 10/08/2014, 05:21

... dopamine neurons from human embryonic stem cells Proc Natl Acad Sci USA 2004, 101:12543-12548 Ying QL, Stavridis M, Griffiths D, Li M, Smith A: Conversion of embryonic stem cells into neuroectodermal ... negative regulators of self-renewal of ES cells It is recently documented that ERK2 null ES cells fail to commit into neural and mesodermal cells [21-24] Similarly, rare brachyury (T) expressed cells ... self-renewal of mouse embryonic stem cells Dev Biol 1999, 210:30-43 Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells Dev Growth Differ...

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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps

Ngày tải lên : 10/08/2014, 05:21

... oxygen depletion/reoxygenation conditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated ... the conserved catalytic active site in cultured mouse embryonic stem cells Due to the importance of anti-oxidative stress mechanisms in stem cells, we have performed studies on this isoform in ... cultured embryonic stem cells using the primer pairs of: MsrA-for: 5’-cctggctgcggaggtggagaaac and MsrA-rev: 5’-atggccatcgggcaggaaactcc The 744bp DNA band was gel purified and ligated into pGEM-T...

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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt

Ngày tải lên : 11/08/2014, 20:21

... of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10:1518-1525 17 Chaudhry GR, Yao D, Smith A, Hussain A: Osteogenic Cells Derived From Embryonic Stem Cells Produced ... potential of embryonic stem cells [30] In this respect, fusion of multiple bony units may allow the reconstruction of larger skeletal elements Through the ability of embryonic stem cells to differentiate ... Luo K, Link C: Embryonic stem cells as an alternate marrow donor source: engraftment without graft-versushost disease J Exp Med 2004, 199:895-904 36 Zavazava N: Embryonic stem cells and potency...

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báo cáo khoa học:" Induction of osteogenic markers in differentially treated cultures of embryonic stem cells" ppt

Ngày tải lên : 12/08/2014, 01:22

... autologous cells as well as allogenic and xenogenic cells [13-16] There are some reports that use totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic stem cells (ESCs) ... which was Figure stem cells (ESCs) Mineral deposition at day 14 in differently treated embryonic Mineral deposition at day 14 in differently treated embryonic stem cells (ESCs) Cells were exposed ... pluripotency in mouse embryonic stem cells Cloning Stem Cells 2004, 6(4):386-391 Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D: Inhibition of pluripotential embryonic stem cell differentiation...

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