cell growth and neoplastic transformation of cells derived from the telomerase knockout mouse fibroblasts versus embryonic stem cells
Báo cáo y học: " Functional and phenotypical comparison of myofibroblasts derived from biopsies and bronchoalveolar lavage in mild asthma and scleroderma" ppsx
Ngày tải lên :
12/08/2014, 16:20
... for 200 cells from the border of the removed cylinder Stress fiber analysis For stress fiber analysis, cells were seeded (5000 cells/ well) under the conditions described above Thereafter, cells ... [12] Therefore, the cells were analyzed for expression of the myofibroblast marker α-SMA to study the phenotype of the cultured fibroblasts The fibroblasts expressed α-SMA in both groups of patients ... Expression of RhoA and Rac1 in fibroblasts from BALF and bronchial biopsies from patients with SSc and mild asthma Figure Expression of RhoA and Rac1 in fibroblasts from BALF and bronchial biopsies from...
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Báo cáo y học: "Impaired nuclear import and viral incorporation of Vpr derived from a HIV long-term non-progressor" docx
Ngày tải lên :
13/08/2014, 05:21
... to the F72L mutation, the levels of observed CD4+ T -cells within the LTNP may be due to the inability of HIV lacking Vpr to import the viral genome into the non-dividing host cell nucleus and ... Visualization of the intracellular behavior of HIV in living cells J Cell Biol 2002, 159:441-452 Piller SC, Caly L, Jans DA: Nuclear import of the pre-integration complex (PIC): the Achilles heel of HIV? ... expression of the β-gal protein Non-infected cells remain colourless due to the lack of Tat expression and subsequent activation of the β-gal gene Data presented depicts relative levels of infectivity...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
Ngày tải lên :
20/02/2014, 02:21
... to their positions The arrowheads depict the residues important for the structural core of the IL-10 gene The underlined amino acid residues are the signal sequences of the respective genes The ... control of the amount and quality of cDNA product was present at all time-points of incubation, except for the h control The highest level of expression was seen at h post-LPS stimulation of liver cells ... isolated and its genomic structure and expression analysis investigated This work will pave the way for further investigation of the biological function of this gene, and the probability of the presence...
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Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx
Ngày tải lên :
07/03/2014, 15:20
... and sequenced as above The fulllength cDNA of lac1 was then generated by 3¢-RACE using the primer 5¢-TCTCAACCGTCGACAGCAG TGTTCGTG-3¢ designed from the sequence of the extreme 5¢ end of lac1 The ... purified and characterized one of these laccases, lac1, and cloned and sequenced the cDNA encoding the protein RT-PCR was used to study the regulation of lac1 gene expression in V volvacea when the ... out by analysis of the deduced amino acid sequence of the enzyme Thus, the 10 histidines and one cysteine residue required to coordinate the four copper atoms at the active site of the enzyme [32]...
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Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt
Ngày tải lên :
17/03/2014, 10:20
... degraded to cellobiose and cellotriose (Fig 2B) Production of cellobiose and cellotriose from cellopentaose by degradation is natural but production of cellobiose and cellotriose from cellotetraose ... combined to the 3¢-end of the oligodT primer for first-strand synthesis of cDNA [12], and 5¢-RACE amplification was performed with a gene-specific primer based on the sequence of the 3¢-fragment The nucleotide ... amino-acid sequence of P hilaris cellulase Alternatively, the mobility of the P hilaris cellulase protein on SDS/PAGE may be different from those of the standard proteins used in the experiment A...
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Báo cáo khoa học: Isolation and identification of antimicrobial components from the epidermal mucus of Atlantic cod (Gadus morhua) docx
Ngày tải lên :
23/03/2014, 15:21
... of antimicrobial components The aim of the present study was to identify antimicrobial components from the skin mucus of healthy Atlantic cod (Gadus morhua) The mucus extract collected from the ... Antimicrobial components of cod mucus A B Fig Purification of antimicrobial components from 20 mg protein ⁄ peptide extract, prepared from the skin mucus of cod, by use of HPLC and monitoring the antibacterial ... mimic the natural environment of cod further supports the role of the mucus as a defence barrier Because we observed an abolition of the extract activities by pepsin treatment we concluded that the...
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Tài liệu Growth and nutritional status of children with homozygous sickle cell disease ppt
Ngày tải lên :
12/02/2014, 19:20
... years of age and compared with normal AA controls, showed no birthweight differences for either gender; the weight deficit in the SS children commenced before the end of the 1st year of life.32 The ... in boys and 0.6 years in girls By the age of 8, this had increased to and 1.3 years in boys and girls, respectively Children with SC disease showed no growth deficit.32 The time of the growth ... below the reference cut-off, and there were significant positive associations between PLP levels and BMI Z-scores, weight and MUAC.111 Reduced levels of other B vitamins including B12112 and riboflavin113...
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Báo cáo sinh học: "Differences in the way a mammalian cell and yeast cells coordinate cell growth and cell-cycle progression" ppt
Ngày tải lên :
06/08/2014, 18:20
... where the sizes of two, unequally sized, hypothetical daughter cells are followed through several cell cycles If the cells and their progeny grow and progress through the cell cycle at the same ... the cells enlarged, and the growth curve would be exponential-like [11] To determine cell- growth rate, we measured the volume of cells in parallel cultures every 24 hours after removing the cells ... removing the cells from the culture dish with trypsin at the time of passage As expected, the average size of the cells remained unchanged with repeated passaging (Figure 2) Schwann cell growth...
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Báo cáo y học: "Functional characterization of Trip10 in cancer cell growth and survival" ppsx
Ngày tải lên :
10/08/2014, 05:21
... by the fact that CIP4a does not contain the TC10-binding domain Therefore, the differential effects of Trip10 in IMR-32 cells and CP70 cells may result from different isoforms in these two cell ... a cell type-specific manner Among the cell lines we examined, the DNA methylation level of Trip10 (from highest to lowest) was: brain tumor cells (IMR-32 and U87) > breast tumor cells (MCF7 and ... in the cytosol and perinuclear region of IMR-32 control cells, but was more evenly distributed in the cytosol of CP70 control cells (Figure immunostaining) Overexpression of Trip10 in IMR-32 cells...
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báo cáo khoa học: " MicroRNAs involved in neoplastic transformation of liver cancer stem cells" pot
Ngày tải lên :
10/08/2014, 10:20
... SP cells are enriched for markers of HSCs To examine whether SP cells are enriched for characteristics of stem cells compared to the non-SP cells, we further characterized the SP cells from the ... identified in the P3 gate on the flow cytometry profile after the cells were stained with Hoechst 33342, (B and D): The SP cells in both HCC cells and fetal liver cells disappeared (0.0%) when cells ... Analysis of stem cell marker expression on the surfaces of SP and non-SP cells The number within each histogram represents the percentage of CD90.1 positive cells (I-K) Quantitative analysis of AFP and...
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báo cáo khoa học: "Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin" pot
Ngày tải lên :
11/08/2014, 11:21
... upper semicell after the second cell division (E) Cell line 18 Upper semicell resulting from the first cell division, after which the cell died (F-I) Cell line 13 Loss of growth polarity and planarity ... isolated and further analysed (Additional file 13) A group of cells lost the GFP-fluorescence within a few days and divided, resulting in normal daughter cells, while the majority of the cells died, ... composition and structure of the cell wall that governs the cell expansion direction and rate As in land plants, the primary cell wall of M denticulata Bréb consists mainly of pectins [10,11], cellulose...
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Báo cáo y học: " Peptides derived from the HIV-1 integrase promote HIV-1 infection and multi-integration of viral cDNA in LEDGF/p75-knockdown cells" doc
Ngày tải lên :
12/08/2014, 04:20
... particularly the absence of any cDNA integration events in such cells is due to the inhibitory effect of Rev [4,6,10,11] Due to the absence of the LEDGF/p75 protein in these cells, all of the IN molecules ... 1640 medium Cells other than the Rev10 + , HeLaP4/ shp75Cl15 and Sup-T1/TL3 cells were provided by the NIH Reagent Program, Division of AIDS, NIAID, NIH (Bethesda, MD, USA) The various cells were ... LEDGF/p75-knockdown cells and in the presence of the INr peptides, the same level as in infected, non-treated, WT HeLa P4 cells (Fig 5) Furthermore, even higher levels of p24 and virus production...
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Study of human nasal epithelial stem or progenitor cell growth and differentiation in an in vitro system
Ngày tải lên :
09/09/2015, 11:29
... four types of cell: basal cells, goblet cells and ciliated or non-ciliated columnar cells Basal cells may involve into the epithelial repair after injury and other types of cells are more related ... components and conditions for hNESPCs growth and differentiation in vitro Primary cells from biopsies survived and more than 90% of cells proved to have properties of epithelial stem or progenitor cells ... controls, study of the functional cells showed several distinctions: 1) the intensity and extensity of MUC5AC staining were higher in the cells derived from NP biopsies than in the controls from 10 days...
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Roles of BNIPXL in regulating cell growth and morphology
Ngày tải lên :
16/09/2015, 08:31
... human tissues and cell lines examined, except for the HEK293T embryonic kidney epithelial cells which showed exclusive expression of the β-isoform Interestingly, the expression profile of murine ... all current and past members of the Cell Signaling and Developmental Biology Laboratory, for their help and friendship during the course of this work Namely, Drs Jan Paul Buschdorf and Liu Lihui; ... inactivation of the RhoA pathway concomitant with the activation of the Cdc42/Rac pathways Removal of the proximal region of the its BCH domain (residues 615-644), which resembles the Class I...
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Dual activation of estrogen receptor a and aryl hydrocarbon receptor by the prenylflavone, icaritin restrict breast cancer cell growth and destabilize estrogen receptor a protein
Ngày tải lên :
04/10/2015, 17:05
... (E1) and estriol (E3) However, the most prevalent form of human estrogen is estradiol Estrogens are produced and secreted by the theca and granulose cells of the ovaries The biological actions of ... response The morphology of the ligand binding domain (LBD) of ER, particularly the position of helix 12 is different, depending on the type of ligand that binds to the receptor Genistein binding to the ... ERmediated gene expression and cell proliferation These approaches include the use of anti-estrogens which binds competitively to the ERα and block the activation of the receptor The second approach...
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Phytohormones on cell growth and taxol accumulation via cell culture of taxus wallichiana zucc
Ngày tải lên :
23/10/2015, 15:38
... additives, and natural pesticides from the harvest of the cultured cells and tissues is very feasible The large scale cultivation of tobacco and a variety of plant cells was examined from the late ... variability of growth and paclitaxel content among callus cultures originating from the same type of explants of different mother plants or from different parts of the same mother plant Out of the nine ... plant cell cultures of T cuspidata for the continuous production of taxoids In this case, addition of carbohydrates (sucrose and fructose) in the midway of the growth cycle increased the rate of the...
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Extraction, separation, and bio transformation of natural plant derived compounds within supercritical CO2 environment
Ngày tải lên :
21/05/2016, 22:18
... 12.05 The difference between the solubility parameter of the fluid and the solute (1 and 2) will determine the solubility of a solute in a SCF The smaller the difference, the higher is the solubility ... in one section of the fixed bed, while the extraction from the inside of the particles takes place in the other section 10 Fundamentals and state of knowledge The boundary between the two sections ... mainly in the form of alpha- and betacarotenes (Figure 2.8), the precursor of vitamin A The presence of these carotenes plays an important role in oxidative protection to the oil On the other hand,...
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Tài liệu Fertility, Family Planning, and Women’s Health: New Data From the 1995 National Survey of Family Growth pptx
Ngày tải lên :
12/02/2014, 23:20
... modes of interviewing allows the examination of differences in reporting due to the mode of interviewing (table 26 versus 27, table 28 versus 29, and table 30 versus 31); and the selection of findings ... reported in the NSFG and live births registered on birth certificates in the years 1991–94 In each individual calendar year and for the sum of the years 1991–94, the NSFG estimate of the number of births ... State and included all of the largest metropolitan areas in the United States To obtain a sufficient number of black and Hispanic women for the NSFG from the NHIS sample, all Table A Outline of the...
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Gradual phase and morphology transformation of Fe3O4nanoparticles to a - FeOOH nanorods in alcohol/water mediain the presence of surfactant F127
Ngày tải lên :
19/03/2014, 16:48
... with diameters around 20 nm and lengths up to 200– 300 nm The results of TEM show the nanoparticles are Fe3O4 and nanorods are a-FeOOH The gradual phase transformation from Fe3O4 to a-FeOOH with ... of both Fe3O4 and a-FeOOH appear in XRD pattern, as shown in Fig 1b It reveals the coexistence of two phases in the product From the above results, a gradual phase transformation from Fe3O4 to ... agent to control the mesoscale structure of metal oxides [16–20] The cooperative assembly route was originally developed for the synthesis of mesostructured silica where the simple and effective...
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How Do Earnings Change When Reservists Are Activated - A Reconciliation of Estimates Derived from Survey and Administrative Data docx
Ngày tải lên :
23/03/2014, 02:20
... study, we report on the results of a set of analyses designed to quantify the relative importance of these and other reasons why estimates of earnings changes derived from SOFS-R and administrative ... Estimates of earnings changes derived from SOFS-R and administrative data might differ for a number of reasons The SOFS-R and administrative data differ in the samples of reservists surveyed, the way ... percent in the case of the 2004 SOFS-R and by percent in the case of the 2005 SOFS-R Misreporting of Military Earnings Military earnings before and during activation are consistently higher in the administrative...
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