... containing 1% CaCl2 for 1 min. The dye incorporated into viable cells was extractedwith 50% ethanol containing 1% acetic acid, and absorb-ance at 540 nm was measured.3462 K. Ishihara et al. ... examined the effects of SA on stress response in mammalian cells using a simple screening system, andrevealed that SA is a potent Hsp inducer in mammaliancells, thereby protecting cells against ... HatayamaDepartment of Biochemistry, Kyoto Pharmaceutical University, JapanAs heat shock proteins (Hsps) are involved in protecting cellsand also in the pathophysiology of diseases such as in am-mation,...