Functional and structural study of singapore grouper iridovirus ORF086R

... analysis of the PAH gene in 51 unrelated HPA patients from Southern Italy In addition to the molecular epidemiology of PAH mutations, we characterized the functional properties of two novel mutations ... phenylalanine hydroxylase deciency in Southern Italy: a 96% detection rate with ten novel mutations Ann Hum Genet 71, 1 8519 3 10 Waters PJ (2003) How PAH gene mutations cause hyper-phenylalaninemia and ... analysis of novel mutations Among the mutations identied in our HPA population, two (i.e p.Q301P and c.707-2delA) were novel One of these mutations, p.Q301P, arises from the c.911A>C transversion in...

... structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the presence and the absence of MgATP We used 2D correlation spectroscopy ... degree of homology ( 80%) with the 3280 catalytic domain of other isoforms of PKC belonging to the classical or novel subfamilies However, the percentage identity between the catalytic domain of ... of catalytic domain from PKCf according to Griffiths & Pariente [53], using grams ⁄ 32 Derivation gave the number and position, as well as an estimation of the bandwidth and the intensity of the...

... order: iron(II)HbIV > met-HbIV > met-HbI > iron(II)-HbI The larger peroxidative activity of iron(II)-HbIV with respect to metHbIV is in line with previous data obtained with human HbA derivatives [7] ... [6] Figure shows the peroxidase activity of iron(II)- and met-forms of HbI and HbIV It is evident that the enzymatic activity decreases according to the order iron(II)-HbIV > met-HbIV > met-HbI ... indicating that tetramers with high-spin ligand water of iron(III) subunits, show the most T-state behavior [38] CD spectra show that iron(II)- and iron(III)-HbI structural features are almost insensitive...

... kinetics and mechanism of liver alcohol dehydrogenase with primary and secondary alcohols as substrates Biochem J 100, 34–46 ´ 36 Branden, C.I., Jornvall, H., Eklund, H & Furugen, B (1975) ¨ ¨ Alcohol ... explain the decrease of CSM for this range of pressure and hence the increase of polarity of the environment of Trp314 Moreover, pressure could also induce a reduction of size of internal cavities, ... pressure stability of this enzyme and perhaps of the other proteins of the alcohol dehydrogenase family Acknowledgements We thank N Bec and R Lange for stimulating discussions and fruitful advice...

... mouse liver: synergism by glutathione depletion and protection by N-acetylcysteine Biochem J 304, 477–483 14 Anderson, M.E (1989) Enzymatic and chemical methods for the determination of glutathione: ... day 15 Similarly, the band shift efficiency in the H2O2-cultures increased by 6.7-fold of the control in 15 days and reached the plateau of 10.7-fold activation on day 20 (Fig 4A) Presence of ... Fauci, A.S (1991) Suppression of human immuno deficiency virus expression in chronically infected monocytic cells by glutathione, gluthathione ester, and N-acetylcysteine Proc Natl Acad Sci USA...

... properties of PLC-d1 and the PLC isoforms at the membrane-associated state through the PS-dependent structural and functional alterations of the PH domains Although the a2-helix located in the b5 ... abolishes these conformational changes of the PH domain and induces the formation of a structure similar to that of the PH domain bound to IP3 The proposed structures of the PLC-d1 PH domain are ... from the ratios of the PH domain partitioned into the supernatants and the precipitates corresponding to the free PH domain and PH domain PIP2 complex, respectively Affinities of the PLC-d1 PH domain...

... deposit, a thorough chemical and structural analysis at the nanoscale has been performed by means of EELS and HRTEM Together with the chemical analysis of the inner part of each deposit, spatially ... quantification of the relative amounts of each element, with a collection angle of 24 mrad and a convergence angle of 7.5 mrad Both types of experiments had an acquisition time of 0.8 s/spectrum Results and ... where a 19% O content is observed On the other hand, to understand the oxidation state of Co, the study of the L2,3 edge of cobalt and the K edge of oxygen in the EELS spectra can be very useful...

... Chloriridovirus and, Lymphocystivirus and Ranavirus (Chinchar et al., 2005) 1.3 Introduction of Singapore grouper iridovirus (SGIV) In 1994, a novel member of Ranavirus, Singapore grouper iridovirus ... …………………………………………………………….……27 Chapter iTRAQ study of Grouper embryonic cells infected by Singapore grouper Iridovirus: an insight into the Singapore grouper iridovirus and host cell interaction……………………………….… ... Introduction of Iridovirus ……………………………………………… 1.3 Introduction of Singapore grouper iridovirus (SGIV)………………… 1.4 Transcriptional regulation & Replication cycle of the iridovirus …… 1.5 Functional genomics ……………………………………………………...

... Sea bream iridovirus Taiwan grouper iridovirus Olive flounder iridovirus Rock bream iridovirus White sturgeon iridovirus Unassigned 1.1.2 Structure of the Iridoviruses Iridoviruses are icosahedral ... mass fingerprinting Q-TOF quadrupole-TOF RACE rapid amplification of cDNA ends RBIV rock bream iridovirus RNAi RNA interference RNase ribonuclease SGIV Singapore grouper iridovirus SOR hydroxysteroid ... Overview of the DNA virus Family Iridoviridae 1.1.1 Classification of the DNA virus Family Iridoviridae 1.1.2 Structure of the Iridoviruses 1.1.3 Previous Functional Genomics...

... ds DNA 13 3894 bp 15 4 ORFs L22858 Ayres et al., 19 94 ds DNA 13 9342 bp 14 1 ORFs AF32 515 5 Pang et al., 20 01 ds DNA 15 8482 bp 16 8 ORFs AY126275 Li et al., 2002 ds DNA 12 8 413 bp 13 6 ORFs NC_0 019 62 Gomi ... Yes Putative E3 Singapore grouper iridovirus SGIV Ranavirus 14 013 1 16 2 AY5 216 25 Yes Putative E3 Infectious spleen and kidney necrosis virus ISKNV Megalocystivirus 11 1362 12 4 AF3 719 60 No Putative ... type Iridovirus 19 110 0 12 6 DQ643392 No Putative E3 Invertebrate iridescent virus IIV type Chiloriridovirus 212 482 468 AF3037 41 No Putative E3 Grouper Iridovirus GIV Ranavirus 13 9793 12 0 AY666 015 ...

... gi|566 926 44 ORF007L ORF012L SGIV protein 323 09 .26 iTRAQ ratio (MO18/ MOctrl) 0.379483 gi|566 926 49 ORFs/ Protein name Protein MW (Da) 129 768.6 0.6634 02 2 629 8 .25 0.579507 23 111.84 0.573601 gi|566 926 83 ... C., Wang F., and Hew C L 20 04 Functional genomics analysis of Singapore grouper iridovirus: complete sequence determination and proteomic analysis Journal of Virology 78: 125 76- 125 90 Song, W., ... Chang, C.Y 20 05 Complete genome sequence of the grouper iridovirus and comparison of genomic organization with those of other iridoriviruses Journal of Virology 79, 20 10 -20 23 Twyman, R.M 20 04 Principles...

... nutritional and functional properties of the major protein fraction was mapped out Finally, the functional and structural properties of the 11S and 7S were contrasted with those of the 2S fraction of soy ... extraction of 11S caused the 11S to have higher level of isoflavone, better foaming properties and formed harder gels I The functional and structural properties of 2S soy protein in relation to other molecular ... foaming and emulsifying properties of the protein system The chapter of the study involved the study of the foaming and emulsifying properties of 2S soy protein in relation to other molecular protein...

... FUNCTIONAL AND STRUCTURAL STUDY OF SINGAPORE GROUPER IRIDOVIRUS ORF086R YAN BO (B.Sc., Xiamen University,China) A Thesis Submitted For The Degree Of Master Of Science Department of Biological ... -55 Figure3.20 CD study of ORF086R( 1-85) 57 Figure3.21 DLS study of ORF086R( 1-85) 57 Figure3.22 1D NMR study of ORF086R ... process of mature virus Due to the availability of cell line, we have decided to take a functional and structural study of SGIV The objectives of this project are: 1) To discover the functions of...