congenital and structural abnormalities of the liver

Tài liệu Báo cáo Y học: Puriﬁcation, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

Ngày tải lên : 22/02/2014, 07:20

... at their 50 and 30 boundaries, respectively, and were inserted between the third letter of one codon and the ﬁrst letter of the following codon Molecular modeling of the C sepium b-amylase The ... the X-ray coordinates of the soybean b-amylase (Fig 4) According to the Ramachandran plot of this model the f and c angles of most of the residues are in the allowed regions of low energy, except ... Cys97, Cys208 and Cys343) On the analogy of the soybean b-amylase, the active site of the C sepium b-amylase most probably consists of a cleft located between the barrel core and the smaller globular...

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611
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Báo cáo khoa học: Puriﬁcation and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

Ngày tải lên : 07/03/2014, 12:20

... sequence and sequence alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the ﬁgure indicate the positions of the nucleotide sequence The ... in the pollen As a consequence, individuals allergic to BGP require individual diagnosis and therapy, and an understanding of the structure of these allergens is essential to the improvement of ... sequences of the peptides, the position in the complete sequence of Cyn d 24 is indicated the amino acids of number and number peptide sequences (Fig 2B), and an antisense primer, designed on the basis...

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665
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Báo cáo khoa học: Puriﬁcation and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

Ngày tải lên : 07/03/2014, 15:20

... component bands, determined by band ﬁtting of the absorbance spectrum of Fig 4A, are shown in Fig and Table From Table 2, the sum of individual amide I¢ intensity and the intensity percent of each ... indicated by the presence of a very strong negative band at 198 nm The negative band near 220 nm results from overlapping of the bands of b-sheet (215 nm) and a-helix Table Fractional composition of secondary ... studies of the catalytic subunit of the cAMP-dependent protein kinase Its ability to readily alter the PKI coding sequence could permit further studying of human PKIb and understanding of the function...

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531
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Báo cáo khoa học: Mercury(II) binding to metallothioneins Variables governing the formation and structural features of the mammalian Hg-MT species pptx

Ngày tải lên : 16/03/2014, 18:20

... on the effect of two variables, the reaction time and the presence of chloride ions, on the stoichiometry, stereochemistry and degree of folding of the Hg(II)-MT species formed by either the ... of all the variables in the Hg-MT system required the performance and analysis of 18 Ó FEBS 2004 titrations and the corresponding duplicates The detailed and comparative analysis of the set of ... Notwithstanding this, there is a minor effect of t and X at the beginning and end of the titration Thus, the addition of the ﬁrst 4–6 of Hg(II) to apo-MT gives rise to Hg-MT species of comparable...

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Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis pptx

Ngày tải lên : 17/03/2014, 17:20

... cloning of the gene encoding the secreted CM from Yersinia pestis (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the ... form, and the rest of the structure is in cartoon form In EcCM, one polypeptide chain is gray and the other is rose In 90-MtCM, one polypeptide chain is blue and the other is green The TSA from the ... performing the role of Gln88 in EcCM Of the other two structures, PfCM has the conserved Ser70 and Gln74, and TtCM has Ser81 and Glu85 There are two Protein Data Bank files for TtCM; in the file 2D8D,...

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513
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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx

Ngày tải lên : 23/03/2014, 13:20

... et al of the ndh genes is much higher in BS chloroplasts, and elevated amounts of the Ndh complex have been found in these plastids [18] The function of the Ndh complex is still a matter of debate ... about the viability of ndh mutants [2,23,25] have clearly restarted the debate about the real function(s) of the Ndh complex To contribute to the structural and functional characterization of this ... characterize the Ndh complex, the MS and BS chloroplasts were separated on CN ⁄ PAGE (1D, based on the internal charge of the protein complexes) and BN ⁄ PAGE (2D, based on the external charge of the...

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431
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Báo cáo khoa học: Phosphatidylserine induces functional and structural alterations of the membrane-associated pleckstrin homology domain of phospholipase C-d1 docx

Ngày tải lên : 30/03/2014, 10:20

... (located at the C-terminus of the a2-helix) and Ala112 (located at the C-terminus of the b7-strand connected with the base of the b5 ⁄ b6 loop by hydrogen bonds) The conformational change of Ala112 ... the loss of the interaction of the a2-helix with membrane interface) is one of the factors that destabilize the membrane binding state of the PH domain As shown in Table and Fig 2, addition of ... heterogeneity of Ala112 in the PH domain bound to PC ⁄ PIP2 vesicles The chemical shift and the line shape of the 18.44 p.p.m peak are similar to the chemical shift and line shape of the peak representing...

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425
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Báo cáo khoa học: Role of the surface charges D72 and K8 in the function and structural stability of the cytochrome c6 from Nostoc sp. PCC 7119 potx

Ngày tải lên : 30/03/2014, 20:20

... K8 and D72 are located near the crossing region of the helices, and the Nx atom of K8 is found ˚ at a distance of 6.2 A from the nearest side-chain oxygen of D72 Taking the functionally and structurally ... neutralization of the effect of the mutation D72K on the protein’s global charge was not sufﬁcient to fully reverse the negative effect on the stability of cyt c6 The apparent stability of the mutants ... the mutation of surface residues located far from the heme moiety Thus, the thermodynamic driving force for the reaction of cyt c6 with PS I was essentially unaltered by the introduction of the...

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469
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Ngày tải lên : 15/02/2014, 01:20

... II The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The ... Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ribose are involved in ... Asp142, 2¢ and 3¢OH of the ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino...

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770
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modiﬁed protein and yields fragments with low immunoreactivity pptx

Ngày tải lên : 21/02/2014, 15:20

... of the structural relationship of CP1/CP2 and TP1/TP2 with the remainder of the BLG structure It is evident that all these fragments include a generous portion of the b-barrel structure (strands ... amount of time At the end of the heat treatment the mixtures were placed in an ice/water bath, and the enzymatic activity was stopped by lowering the pH of the reaction mixture to by addition of ... structure (strands B, C and part of strand D), along with part of the distant I strand in the C-terminus region that includes Cys160 and connects to the leftovers of strand D via a disulﬁde bridge...

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526
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Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf

Ngày tải lên : 22/02/2014, 07:20

... 2001 cleavages of the sites were in the order: Tyr146/Asn147 ! Phe114 ! other sites The weakness of bands of fragments I and IV and the appearance of fragment II in the degradation of the Tyr146 ... 2) The cleavage of the interdomain and autolysis loops To follow the autolysis of D-chymotrypsin and the mutants, the gel-electrophoretic patterns of their digests were compared As under the ... (Fig 3B), the order and speed of cleavages are as follows: the rapid cleavage of the Tyr146-Asn147 and/ or the Asn147-Ala148 bond(s) in the autolysis loop precedes the slower hydrolysis of the Phe114-Ser115...

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613
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Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

Ngày tải lên : 07/03/2014, 16:20

... of Pex1p and Pex6p A B Fig Two-hybrid interaction of Pex1p and Pex6p harboring point mutations of the WalkerA and B motifs of their ATP-binding sites A schematic representation of the Pex1p and ... the binding sites of these two AAA-proteins to their different protein regions and elucidated the importance of ATP-binding to the two AAA-cassettes of Pex1p and Pex6p for their interaction The ... to the cytosol Here we conﬁrm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p...

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584
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Báo cáo khoa học: Structural ﬂexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for speciﬁc substrate recognition pptx

Ngày tải lên : 16/03/2014, 06:20

... binding of the substrates The r.m.s.d of Ca atoms after superposition of the catalytic domains in complex with different substrates and the catalytic domain of the apo-enzyme The binding of the small ... nucleophilic attack of the a-phosphate of ATP Thus, according to the crystal structure, the speciﬁcity of serine recognition depends on: (a) the zinc ion, (b) the size of the active site and (c) the hydrophilic ... cognate tRNA Because of the similar size of the motif loop in methanogenic-type SerRS and ThrRS and the involvement of zinc ions in the recognition of amino acid substrates, the M barkeri loop...

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357
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Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot

Ngày tải lên : 16/03/2014, 13:20

... secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the presence and the absence of MgATP We used 2D ... in the width of the amide I¢ band, although the main loss was observed in the 1657 cm)1 component It is of note that the widening of the amide I¢ band (Fig 3) is not so pronounced as in other ... subtracting the spectrum of the solvent from the spectrum of the protein in the same solvent, in the range 1800– 1500 cm)1, where the amide I and amide II regions can be observed (B) Second derivative of...

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383
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Báo cáo khoa học: Sequences and structural organization of phospholipase A2 genes from Vipera aspis aspis, V. aspis zinnikeri and Vipera berus berus venom Identiﬁcation of the origin of a new viper population based on ammodytin I1 heterogeneity docx

Ngày tải lên : 17/03/2014, 03:20

... 3, and the 5¢ part of exon encode the mature protein; exon encodes the 5¢-UTR, and exon and part of exon encode the signal peptide The 110 nucleotides at the 3¢ end of exon encode the 3¢-UTR The ... Ib in the genome of this snake These ﬁndings provide clues to the evolutionary position of the neurotoxic V a aspis population with respect to the other snakes studied The genome of one of these ... indeed the case, then substitutions occurring in these areas may affect the neurotoxicity or anticoagulant effect of the PLA2 The substitutions in the A and B chains of vaspin, in the inhibitor and...

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451
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Báo cáo Y học: Structural and serological relatedness of the O-antigens of Proteus penneri 1 and 4 from a novel Proteus serogroup O72 pptx

Ngày tải lên : 17/03/2014, 17:20

... by the ratio of the integral intensities of the signals in the major and minor series, the average degree of glycosylation with GlcII is 75% Comparison of the 13C NMR spectra of the initial and ... con®guration of the Gal residue [19] Signi®cant low-®eld displacements of the signals for C6 of Glc, C3 and C4 of Gal, and C3 of GalNAcI to d 66.8, 81.3, 77.1, and 81.6 in the 13C NMR spectrum of the ... and GalNAcII H1/Gal H3 at d 4.61/3.96 These data ®tted well with the substitution pattern of the sugar residues determined by the 13C NMR chemical shift data and de®ned the full sequence of the...

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560
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Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx

Ngày tải lên : 23/03/2014, 09:21

... with the native state of the protein We then carried out the characterization of the thermal properties of the mutant in comparison with those of PfPNP The results obtained indicate that the substitution ... oxidation-reduction to rescue the possible damage of the other two disulﬁde bonds The presence of a low percentage of the protein with Cys254 and Cys256 in the reduced form further supports this hypothesis It has ... arrangement of PfPNP The presence of three disulﬁde bonds justify the extreme stability features of PfPNP These covalent links, in fact, lowering the entropy of the unfolded polypeptide and introducing...

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384
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx

Ngày tải lên : 23/03/2014, 13:20

... stainless steel needle (27 g) was butted against the low dead volume tee and enabled the delivery of the sheath solution to the end of the capillary column The separations were obtained on 90-cm long ... comparison of its MS with that of the reference compound The absolute configuration of glycoses was established by capillary GLC of their acetylated (-)-2-butyl glycosides, according to the method of ... amount of 2-amino-2deoxy-D-galactose was identified in the hydrolysis products of both carboxyl-reduced CPS and LPS [19], confirming the presence of GalNAcA in the native CPS and LPS This was further...

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332
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Báo cáo khoa học: The structural comparison of the bacterial PepX and human DPP-IV reveals sites for the design of inhibitors of PepX activity pot

Ngày tải lên : 23/03/2014, 13:20

... group of the ligand and the NH2 of Asn470 (equivalent to Asn710 in DPP-IV) Another potential bond can be proposed between the OH atom of Tyr380 and the carbonyl group of the ligand Finally, the ... lactis The results are presented in Table and Fig 4A For the cluster of lowest energy, both the position and the conformation of the ligand are close to those observed in the 3D structure of the ... comparison of the active and speciﬁcity sites of the two X-PDAP enzymes, the bacterial PepX (green) and the human DPP-IV (orange) (B) Superposition of the active and speciﬁcity sites of the S9 enzymes...

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Báo cáo khoa học: Identiﬁcation and structural characterization of a sialylated lacto-N-neotetraose structure in the lipopolysaccharide of Haemophilus inﬂuenzae pptx

Ngày tải lên : 23/03/2014, 21:21

... excitation of H-1 H-resonance of the GlcNAc residue in the TOCSY step and of H-3/H-4 1H-resonance of the GlcNAc residue in the NOESY step (A) The assignments of the 1H-resonances of the Gal II ... unit is linked to the Hep I residue of the LPS inner core in the sialylated glycoform The structure of the sialylated glycoforms of the O-deacylated LPS of H inﬂuenzae strain RM118 and mutants causing ... shown) and establishing the structure of the tetrasaccharide to be, a-Neu5Ac-(2–3)-b-D-Gal-(1–4)-b-D-GlcNAc-(1–3)-b-DGal Due to the low intensity of the sialylated glycoforms and a high degree of...

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