... both motions ofthe pair The vector ofthe response spectra is calculated as the square root ofthe sum ofthe squares (SSRS) ofthe response spectra for the two different directions, and this vector ... representative ofthe overall loaddeformation behavior ofthe building and compute a response history using appropriate software (e.g., Hachem, 2004) Dynamic analysis ofthe oscillator under thedesign ... While the importance of deformation is paramount in performance-based assessment anddesignof yielding structures, thedesign should not overlook aspects of performance that may be affected by other...
... at their 50 and 30 boundaries, respectively, and were inserted between the third letter of one codon andthe first letter ofthe following codon Molecular modeling ofthe C sepium b-amylase The ... the X-ray coordinates ofthe soybean b-amylase (Fig 4) According to the Ramachandran plot of this model the f and c angles of most ofthe residues are in the allowed regions of low energy, except ... Cys97, Cys208 and Cys343) On the analogy ofthe soybean b-amylase, the active site ofthe C sepium b-amylase most probably consists of a cleft located between the barrel core andthe smaller globular...
... sequence and sequence alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right ofthe figure indicate the positions ofthe nucleotide sequence The ... sequences ofthe peptides, the position in the complete sequence of Cyn d 24 is indicated the amino acids of number and number peptide sequences (Fig 2B), and an antisense primer, designed on the basis ... to the N-terminal portion ofthe allergen Based on this partial sequence, two specific primers were designed and used together with the anchor primer AP2 to obtain the 5¢ and 3¢ portions of the...
... indicated by the presence of a very strong negative band at 198 nm The negative band near 220 nm results from overlapping ofthe bands of b-sheet (215 nm) and a-helix Table Fractional compositionof secondary ... component bands, determined by band fitting ofthe absorbance spectrum of Fig 4A, are shown in Fig and Table From Table 2, the sum of individual amide I¢ intensity andthe intensity percent of each ... studies ofthe catalytic subunit ofthe cAMP-dependent protein kinase Its ability to readily alter the PKI coding sequence could permit further studying of human PKIb and understanding ofthe function...
... on the effect of two variables, the reaction time andthe presence of chloride ions, on the stoichiometry, stereochemistry and degree of folding ofthe Hg(II)-MT species formed by either the ... of all the variables in the Hg-MT system required the performance and analysis of 18 Ó FEBS 2004 titrations andthe corresponding duplicates The detailed and comparative analysis ofthe set of ... Notwithstanding this, there is a minor effect of t and X at the beginning and end ofthe titration Thus, the addition ofthe first 4–6 of Hg(II) to apo-MT gives rise to Hg-MT species of comparable...
... 3, andthe 5¢ part of exon encode the mature protein; exon encodes the 5¢-UTR, and exon and part of exon encode the signal peptide The 110 nucleotides at the 3¢ end of exon encode the 3¢-UTR The ... Ib in the genome of this snake These findings provide clues to the evolutionary position ofthe neurotoxic V a aspis population with respect to the other snakes studied The genome of one of these ... indeed the case, then substitutions occurring in these areas may affect the neurotoxicity or anticoagulant effect ofthe PLA2 The substitutions in the A and B chains of vaspin, in the inhibitor and...
... cloning ofthe gene encoding the secreted CM from Yersinia pestis (*YpCM, y2828), purification ofthe protein, investigation ofthe properties ofthe enzyme, andthe crystal structure analysis ofthe ... form, andthe rest ofthe structure is in cartoon form In EcCM, one polypeptide chain is gray andthe other is rose In 90-MtCM, one polypeptide chain is blue andthe other is green The TSA from the ... performing the role of Gln88 in EcCM Ofthe other two structures, PfCM has the conserved Ser70 and Gln74, and TtCM has Ser81 and Glu85 There are two Protein Data Bank files for TtCM; in the file 2D8D,...
... with the native state ofthe protein We then carried out the characterization ofthe thermal properties ofthe mutant in comparison with those of PfPNP The results obtained indicate that the substitution ... oxidation-reduction to rescue the possible damage ofthe other two disulfide bonds The presence of a low percentage ofthe protein with Cys254 and Cys256 in the reduced form further supports this hypothesis It has ... arrangement of PfPNP The presence of three disulfide bonds justify the extreme stability features of PfPNP These covalent links, in fact, lowering the entropy ofthe unfolded polypeptide and introducing...
... et al ofthe ndh genes is much higher in BS chloroplasts, and elevated amounts ofthe Ndh complex have been found in these plastids [18] The function ofthe Ndh complex is still a matter of debate ... about the viability of ndh mutants [2,23,25] have clearly restarted the debate about the real function(s) ofthe Ndh complex To contribute to thestructuraland functional characterization of this ... characterize the Ndh complex, the MS and BS chloroplasts were separated on CN ⁄ PAGE (1D, based on the internal charge ofthe protein complexes) and BN ⁄ PAGE (2D, based on the external charge of the...
... excitation of H-1 H-resonance ofthe GlcNAc residue in the TOCSY step andof H-3/H-4 1H-resonance ofthe GlcNAc residue in the NOESY step (A) The assignments ofthe 1H-resonances ofthe Gal II ... unit is linked to the Hep I residue ofthe LPS inner core in the sialylated glycoform The structure ofthe sialylated glycoforms ofthe O-deacylated LPS of H influenzae strain RM118 and mutants causing ... shown) and establishing the structure ofthe tetrasaccharide to be, a-Neu5Ac-(2–3)-b-D-Gal-(1–4)-b-D-GlcNAc-(1–3)-b-DGal Due to the low intensity ofthe sialylated glycoforms and a high degree of...
... (located at the C-terminus ofthe a2-helix) and Ala112 (located at the C-terminus ofthe b7-strand connected with the base ofthe b5 ⁄ b6 loop by hydrogen bonds) The conformational change of Ala112 ... the loss ofthe interaction ofthe a2-helix with membrane interface) is one ofthe factors that destabilize the membrane binding state ofthe PH domain As shown in Table and Fig 2, addition of ... for the PH domain and consequently for PLC-d1 Dynamic changes in the lipid compositionofthe plasma membrane would be capable of providing a means of regulating the properties of PLC-d1 and the...
... K8 and D72 are located near the crossing region ofthe helices, andthe Nx atom of K8 is found ˚ at a distance of 6.2 A from the nearest side-chain oxygen of D72 Taking the functionally and structurally ... neutralization ofthe effect ofthe mutation D72K on the protein’s global charge was not sufficient to fully reverse the negative effect on the stability of cyt c6 The apparent stability ofthe mutants ... along the C-terminal a helix to E68 and Y76 Interestingly, one ofthe side chain oxygens of D72 appears to be hydrogen-bonded to the backbone N atom of G6, bridging the crossing between the N- and...
... interfering with the reaction between the endotoxins andthe LAL As a consequence, the suitability ofthe regents and materials used andthe product itself has to be established The endotoxin limit ... of micro-organisms is obtained after incubation in presence of blood andthe ATMP, the product possesses no antimicrobial activity under the conditions ofthe test, andthe sterility may be then ... and coordination All the authors read and approved the final manuscript References Regulation (EC) No 1394/2007 ofthe European Parliament andofthe Council of 13 November 2007 on advanced therapy...
... due to the geographical location andthe harvesting period It is noteworthy that the results of this study may be considered as the first report on thecompositionofthe essential oils of this ... order of their elution on the HP-5MS non-polar column (Table 1) A total of 19 compounds were identified, representing 86.68% ofthe total oil The esters made up the largest component ofthe oil ... [4] andthe neuropharmacological properties [5] have been investigated as well To the best ofthe authors’ knowledge, there are no reports about the chemical content and biological effect of the...
... due to the geographical location andthe harvesting period It is noteworthy that the results of this study may be considered as the first report on thecompositionofthe essential oils of this ... order of their elution on the HP-5MS non-polar column (Table 1) A total of 19 compounds were identified, representing 86.68% ofthe total oil The esters made up the largest component ofthe oil ... [4] andthe neuropharmacological properties [5] have been investigated as well To the best ofthe authors’ knowledge, there are no reports about the chemical content and biological effect of the...
... noteworthy that the results of this study may be considered as the first report on thecompositionofthe essential oils of this endemic species Chahma A voucher specimen was deposited at the herbarium ... was found between the oil content of L resedifolia and that of L arboresens as was reported by Cheriti et al [6] The slight difference may be due to the geographical location andthe harvesting ... reported in the literature [12] and stored on the MS library (NIST database) The concentration ofthe identified compounds was computed from the GC peak total area without any correction factor The compounds...
... for at 70 ◦ C, then mixed with a 5× first-strand buffer, 10 mM dNTPs, and 0.1 M DTT The reaction mixture was further incubated for at 37 ◦ C, then for 60 after the addition of U of MuLV reverse ... fractions The yield ofthe neutral fraction (CPSN) andthe acidic fraction (CPSA) obtained from the crude polysaccharide extract CPS was 0.328 g/g and 0.034 g/g, respectively (Fig 1A) The molecular ... analysis of CPSN Fr II and standard polymers according to the absorption maximum ofthe congo red-polysaccharide complex at various concentrations of NaOH For more details, see Section (C–H), and...
... limitant, andthe sand-silty and acid soil favour chestnut growth in the Park, according to the results of Bernetti [5], Leonardi et al [34] and Rubio and Escudero [54] The higher SOM and Influence of ... persistence of other problems associated to this perturbation, mainly leaching The correlation analysis (Tab III) underlined the dependence of LAI on the considered structural traits ofthe stands In ... stool andthe invigorating effects of decapitation which could cause an initially fast growing ofthe coppice sprouts, according to the results of Kauppi and Kiviniitty [30] and Rinne et al [52] These...
... drafting ofthe manuscript MA contributed with plant material, the general idea ofthe study and participated in revision ofthe manuscript AKHE participated in the general idea ofthe study, thedesign ... to the growing ofthe plants, harvested materials, carried out thestructural examination and drafted the manuscript YKL participated in designing the experiments, structural analysis andthe ... controlling the abscission event of pea seeds Absence ofthe hilum palisade layers is the key characteristic in the def mutant pea seed Structural analysis revealed the absence ofthe palisade layer and...
... compromising thestructural integrity ofthe core domain On the other hand, when the modified residues are part ofthe hydrophobic core ofthe domain, the modification could cause considerable structural ... Phosphorylation of T432 may affect the relative orientations between domains within the protein, whereas Y29 may modulate the conformation ofthe helical subdomain The orientations ofthe domains andofthe ... 5% ofthe sites were strictly conserved across the alignments, and not more than 20% ofthe sites were conserved in at least 80% of all ofthe homologs within the alignments As expected, the...