... substrate-freeAppA the C a atoms are 2.41 A ˚apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1.87 A ˚.Distinct conformational changes were ... December2009)doi:10.1111/j.1742-4658.2010.07559.x The extracellular phytase ofthe plant-associated Klebsiella sp. ASR1 is a member ofthe histidine-acid-phosphatase family and acts primarily as a scavenger of phosphate groups locked in the ... a ⁄ b domain than in thea domain [20].This is also reflected in the rigidity ofthe protein. The atomic displacement factors ofthe PhyK structure aresmaller for thea ⁄ b domain than for the...
... quantitative and qualitative assays for b-galactosidase (activation of LacZ reporter gene). BD, pGBT9; BD*, pAS2-1; AD, pGAD424;AD**, pACT2. Activity values are given as mean values ± standard ... PCR-amplified from the DNA ofthe cosmid clone #9 [18] using the following pair of primers: 5Â-GACTGCAGTGAAGGGCATCGAGTCCTCGGG-3Â and 5Â-GAGGATCCGGGACATTCCTTAGCCAGGAGGG-3Â.Tomaketheb-galactosidase expressing ... different organisms, is responsible for the downregulation of catalytic activity ofa subunit towardcalmodulin and for the activation by polybasic compounds. The examination of amino-acid alignment...
... regulatory treatment of cable andwire-line broadband services are around the corner. The FCC hasalready ruled that the cable broadband falls under Title I ratherthan Title II.Absent another ... ideas today.Through all the technical discussions, all that technical FCC andPUCA rigmarole, if we can just all agree that in a broadband worldit is all the same and Americans ought to have access ... competition across all plat-forms for broadband services. The Commission’s regulatory framework conceptual-izes broadband to include any and all platforms capable of combining the power of communications...
... conformations ofthe b-amino acidsbackbone, corresponding to gauche rotamers around the Ca–Cb bond, can overlap canonical backbone conformersobserved for a- amino acids. Therefore the addition of ... expressed as Kifor NK-1M (major site) and NK-1m (minorsite), and EC50values for the cAMP pathway and for the inositol phosphates pathway.SP, [Ala9]SP and [Pro9]SP are almost equipotent at the major ... potentialenergy surfaces the conformers of b-amino acids that fitwith canonical conformations of a- amino acids, two(/ ) w) Ramachandran maps corresponding to h torsionangle values of +60°,gauche(+) ,or) 60°,...
... you change the DataColumn in the parent DataTable on which the ForeignKeyConstraint was created, then the same change is also made in any corresponding DataRow objects in the child DataTable. ... in the child DataTable. This is the default. None Indicates that no action takes place. SetDefault Indicates that the DataColumn values in the child DataTable are to be set to the value in the ... stored in a DataTable named ordersDT. ã The customersDT and ordersDT DataTable objects are related to each other using the following DataRelation: ã DataRelation customersOrdersDataRel = ã...
... catalyticdomain from BsAKII and regulatory domains (a regulatory domain in the a- subunit, and a b-subunitwith the same sequence as theregulatory domain)from TtAK, improved thermostability as much aswild-type ... step in the archaeal aspartate biosyntheticpathway catalyzed by a monofunctional aspartokinase.Acta Crystallogr F 62, 962–966.20 Yoshida A, Tomita T, Kurihara T, Fushinobu S,Kuzuyama T & ... total were used for datafitting. The subunit organization of TtAKb was analyzedby nonlinear least square analysis using the softwarepackage origin 2.8, provided by Beckman, with the partialspecic...
... oAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcus119119119119119120119121118119159159159159157158157160158159199199199199197198197200198199VI ... (Fig. 1A) , and that of chloroplast spinach GAPDH [35] w ere examined t oAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumB A. thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcus119119119119119120119121118119159159159159157158157160158159199199199199197198197200198199VI ... (1999) AMBER 6.University of California, San F rancisco, CA.34. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) MolecularCloning: A Laboratory Manual. 2nd edn. Cold Spring HarborLaboratory,...
... link the senses acrossdictionaries, hence Wik is only used as augmenteddata for WMF to better learn the semantics of words.All data is tokenized, POS tagged (Toutanova et al.,2003) and lemmatized, ... (adjectives and adverbs) do not have a taxonomic representation structure. For example, the jcn similarity measure (Jiang and Conrath, 1997)computes the sense pair similarity score based on the information ... significantly outperforms previ-ous definition-based similarity measures and LDAvectors on four all-words WSD data sets.AcknowledgmentsThis research was funded by theOf ce ofthe Di-rector of National...
... F. Yamakura and T. Matsumoto for the analyses of iron and manganese in protein samples. We also wish tothank H. Steinman for the gift of E. coli OX32 6A. We finally thank MrM. Farrugia for photographic ... simultaneously afterinoculation of media with cultures grown to exponentialphase in the absence of paraquat and IPTG (Materialsand methods [24]). The final concentration of paraquatand IPTG ... metal in vivo and corresponds to a ratio around 20 times infavour ofthe ÔcorrectÕ metal. This ratio of correct toincorrect metal in the active site ofthe enzyme is greatlyincreasedbymorethantwofoldtogreaterthan50timeswhen...
... 2003 at four time points for each reaction (product formation waslinear for at least 20 min, at all substrate concentrations, atalmost all pH values and for all mutants). In this way kineticparameters ... crystallo-graphy that replacement ofthe Asp142 analogue by alaninein other family 18 chitinases puts the N-acetyl group in a conformation which is not favourable for nucleophilicattack on the ... and Ser93To our knowledge, there are no other mutational data in the literature that address theroleof Tyr10 for catalysis. The importance of Ser93 has previously been shown (but notexplained)...
... structure of an HHMM The models discussed here are evaluatedby applying them to natural language tasksbased on CoNLL-20041and a sub-corpus of the Lancaster Treebank2.Keywords: information extraction, ... only the part -of- speech tags and grammarinformation are considered. The left hand side of the figure shows the original structure ofthe sen-tence, and the right hand side shows the trans-formed ... experimental and theoretical aspects of thiswork is planned, specifically in the area of recon-structing hierarchies where recursive formationsare present and formal analysis and testing of tech-niques.ReferencesD....
... rice and barley a- amylases are thus also presented.MATERIALS AND METHODSSUMAsoftware: subsite mapping of amylasesThis software calculates the apparent binding energies on the basis ofthe measured ... the crystalline state are free or complexed to a substrate-analogue, is a powerful method for mapping the active site of an a- amylase. However, these data can vary according to the crystalline varieties ... Mapping of a- Amylases) is freely availablefor research and educational purposes via the Internet(E-mail: gyemant@tigris.klte.hu). The advantages of this program are demonstratedthrough a- amylases...
... [23]. The substitution of thesehydrogen bond forming residues in hTRXL-N may accountfor the formation ofa monomer, instead ofa dimer in the case of TRX. Furthermore, the loss of intermoleculardisulfide-bonds ... catalyze the reduction of many exposed disulfides.Therefore, thioredoxin can interact with a broad range of proteins either in electron transport for substrate reduction or in regulation of activity ... 32 kDa)can be regarded as a member ofthe mammalianthioredoxin family [27]. The other two members of thisfamily, thioredoxin-1 (m 12 kDa) and mitochondrialthioredoxin-2 (m 18 kDa), are...