explain the regulatory requirements of a sales role
Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
Ngày tải lên :
16/02/2014, 09:20
... December
2009)
doi:10.1111/j.1742-4658.2010.07559.x
The extracellular phytase of the plant-associated Klebsiella sp. ASR1 is
a member of the histidine-acid-phosphatase family and acts primarily as
a scavenger of phosphate groups locked in the ... substrate-free
AppA the C
a
atoms are 2.41 A
˚
apart, whereas for the
substrate-free PhyK and the substrate-loaded AppA
the averaged distance is only 1.87 A
˚
.
Distinct conformational changes were observed ... con-
served active-site motifs, RHGXRXP and HD, and
hydrolyze metal-free phytate with pH optima in the
acidic range. They consist of two domains, a large a ⁄ b
domain and a small a domain with the catalytic...
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Tài liệu Báo cáo Y học: CK2btes gene encodes a testis-specific isoform of the regulatory subunit of casein kinase 2 in Drosophila melanogaster potx
Ngày tải lên :
21/02/2014, 15:20
... quantitative and qualitative assays for b-galactosidase (activation of LacZ reporter gene). BD, pGBT9; BD*, pAS2-1; AD, pGAD424;
AD**, pACT2. Activity values are given as mean values ± standard ... PCR-
amplified from the DNA of the cosmid clone #9 [18] using
the following pair of primers: 5Â-GACTGCAGTGAAGG
GCATCGAGTCCTCGGG-3Â and 5Â-GAGGATCCGG
GACATTCCTTAGCCAGGAGGG-3Â.Tomakethe
b-galactosidase expressing ... the cDNA #911
using the following pair of primers: 5Â-CTGGATCCCT
ATGTCGTGTCCCAGGAGCATCGAG-3Â and 5Â-GTC
TGCAGTTAAAAATTCGGGACATTCCTTAGCCA
GG-3Â. BamHIPstI digested PCR product was cloned as a
fusion...
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
Ngày tải lên :
07/03/2014, 14:20
... glycosyl-
transferase elucidate catalysis in the alpha-amylase family. Nat.
Struct. Biol. 6, 432–436.
16. Terwisscha van Scheltinga, A. C., Armand, S., Kalk, K.H., Isogai,
A. , Henrissat, B. & Dijkstra, ... However, the mechanistic roles of several of
these residues are not described well and there is no example
of mutational analysis of all of these residues in the same
enzyme.
To obtain an insight ... regard to the role of Asp140,
whichappearstobecrucialinChiBandinchitinaseA1
from Bacillus circulans, whereas it may be mutated to
asparagine without loss of activity in other family 18
chitinases...
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The Marketing Strategy of a multinational join stock company.doc
Ngày tải lên :
27/10/2012, 16:51
... The Marketing Strategy of a multinational join stock company
multinational join stock company has a certain advantage: a multinational join stock
company has always kept its prices as competitive ... department is in charge of marketing plans on importing,
material providing and preceding the contracts. Organizing the sales of products is
another main task of the department.
ã Financial and ... stock company size and market share have been small. The
shortage of capital is main cause of the company’s modest size and market share. Up to
now, a multinational join stock company has had only...
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Tài liệu Issues Involved When Updating the Primary Key of a Parent Row pptx
Ngày tải lên :
24/12/2013, 01:17
... in the child DataTable. This is the default.
None Indicates that no action takes place.
SetDefault Indicates that the DataColumn values in the child DataTable are to be set
to the value in the ... you change the DataColumn in
the parent DataTable on which the ForeignKeyConstraint was created, then the same
change is also made in any corresponding DataRow objects in the child DataTable. ...
UpdateRule is set to None.
ã
The CommandText of the Command object in the UpdateCommand of the
DataAdapter is the same as in the second case.
The following code sets the UpdateRule of the...
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Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf
Ngày tải lên :
18/02/2014, 08:20
... that chimeric AK,
BTT, which is composed of the catalytic domain of
BsAKII and the regulatory domain and b-subunit of
TtAK, had thermostability as high as that of wild-type
TtAK, suggesting that ... differential scanning calorimetry; EcAKIII, aspartate kinase III
from Escherichia coli; MAD, multiwavelength anomalous diffraction; MjAK, aspartate kinase from Methanococcus jannaschii; TtAK, aspartate
kinase ... catalytic
domain from BsAKII and regulatory domains (a
regulatory domain in the a- subunit, and a b-subunit
with the same sequence as the regulatory domain)
from TtAK, improved thermostability as much as
wild-type...
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Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc
Ngày tải lên :
19/02/2014, 16:20
... o
AthalianaB
PativumB
SoleraceaB
NtabacumB
A. thalianaA
PsativumA
SoleraceaA
Chlamy
Synechocystis
Synechococcus
AthalianaB
PativumB
SoleraceaB
NtabacumB
A. thalianaA
PsativumA
SoleraceaA
Chlamy
Synechocystis
Synechococcus
AthalianaB
PativumB
SoleraceaB
NtabacumB
A. thalianaA
PsativumA
SoleraceaA
Chlamy
Synechocystis
Synechococcus
119
119
119
119
119
120
119
121
118
119
159
159
159
159
157
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157
160
158
159
199
199
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199
VI ... (Fig. 1A) , and that
of chloroplast spinach GAPDH [35] w ere examined t o
AthalianaB
PativumB
SoleraceaB
NtabacumB
A. thalianaA
PsativumA
SoleraceaA
Chlamy
Synechocystis
Synechococcus
AthalianaB
PativumB
SoleraceaB
NtabacumB
A. thalianaA
PsativumA
SoleraceaA
Chlamy
Synechocystis
Synechococcus
AthalianaB
PativumB
SoleraceaB
NtabacumB
A. thalianaA
PsativumA
SoleraceaA
Chlamy
Synechocystis
Synechococcus
119
119
119
119
119
120
119
121
118
119
159
159
159
159
157
158
157
160
158
159
199
199
199
199
197
198
197
200
198
199
VI ... R A R A A A L N I V P T S T G A A K A VAL V L PNL K G K L N G I A L R V P
R R A R A A A L N I V P T S T G A A K A VAL V L PTL K G K L N G I A L R V P
R R A R A ACL N I V P T S T G A A K A VAL...
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Tài liệu Báo cáo khoa học: "Learning the Latent Semantics of a Concept from its Definition" pptx
Ngày tải lên :
19/02/2014, 19:20
... link the senses across
dictionaries, hence Wik is only used as augmented
data for WMF to better learn the semantics of words.
All data is tokenized, POS tagged (Toutanova et al.,
2003) and lemmatized, ... this case, many senses will share the
same latent semantics profile, as long as they are in
the same topic/domain.
To solve the sparsity issue we use missing words
as negative evidence of latent ... (adjectives and adverbs) do not have
a taxonomic representation structure. For example,
the jcn similarity measure (Jiang and Conrath, 1997)
computes the sense pair similarity score based on the
information...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
Ngày tải lên :
21/02/2014, 01:21
... Peplow, F. Yamakura and T. Matsumoto for the
analyses of iron and manganese in protein samples. We also wish to
thank H. Steinman for the gift of E. coli OX32 6A. We finally thank Mr
M. Farrugia for ... helper phage (Stratagene). One microgram was
utilized in mutagenesis reactions together with oligonucleo-
tides ECF-Q69G d(5Â-AACAACGCAGCT
GGGCTCTG
GAACCAT), ECF -A1 41Q d(5Â-TCAACCTCTAAC
CAG
GCTACTCCGCTG) ... d(5Â-AACAACGCTGG
C
CAGCACGCTAACCAC) and ECM-Q14 6A d(5Â-TCT
ACTGCTAAC
GCGGATTCTCCGCTG) following the
manufacturers instructions (mutagenic nucleotide are
underlined). Mutated plasmids were designated...
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Báo cáo khoa học: Neural retina leucine-zipper regulates the expression of Ppp2r5c, the regulatory subunit of protein phosphatase 2A, in photoreceptor development pdf
Ngày tải lên :
06/03/2014, 02:20
... 5Â-AAA AAA AAG
ACA AAC TGA-3Â; Ppp2r5c promoter (DNRE), forward,
5Â-TAG CAC TTC CTG ACT ATT-3Â; Ppp2r5c promoter
(DNRE), reverse, 5Â-GAA GCT GCA ACT TAA AAT-3Â;
Ppp2r5c promoter (NRE), forward, ... GC-3Â and 5Â-GCC
AGA GGC TGA TTC AGC ATC CGC GAG AT-3Â; oli-
gonucleotides for the Ppp2r5c promoter, 5Â-CCC TGA
AGC CAG GAT GAG CCG CAG GGA AAG-3Â and
5Â-TGG AGC TC G CTG ATT GGC CAG AAG CTG CAA-
3Â) ... 15267–15276.
13 Tanabe O, Nagase T, Murakami T, Nozaki H, Usui H,
Nishito Y, Hayashi H, Kagamiyama H & Takeda M
(1996) Molecular cloning of a 74-kDa regulatory sub-
unit (BÂÂ or delta) of human protein...
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THE REGULATORY STATUS OF BROADBAND SERV- ICES: INFORMATION SERVICES, COMMON CAR- RIAGE, OR SOMETHING IN BETWEEN? docx
Ngày tải lên :
07/03/2014, 11:20
... ideas today.
Through all the technical discussions, all that technical FCC and
PUCA rigmarole, if we can just all agree that in a broadband world
it is all the same and Americans ought to have access ... regulatory treatment of cable and
wire-line broadband services are around the corner. The FCC has
already ruled that the cable broadband falls under Title I rather
than Title II.
Absent another ... and the regulatory approach is fundamen-
tally grounded in a wireline paradigm. In the regulated market, for example, LATA
boundaries matter. In the unregulated market, they do not. The regulated...
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Báo cáo khoa học: "Techniques to incorporate the benefits of a Hierarchy in a modified hidden Markov model" pptx
Ngày tải lên :
08/03/2014, 02:21
... structure of an HHMM
The models discussed here are evaluated
by applying them to natural language tasks
based on CoNLL-2004
1
and a sub-corpus
of the Lancaster Treebank
2
.
Keywords: information extraction, ... Grammar Parsing
Creation of a parse tree involves describing lan-
guage grammar in a tree representation, where
each path of the tree represents a grammar rule.
Consider a sentence from the Lancaster ... 6: The graph of micro-average F -measure
against the number of training sentences during
text chunking (A: MHHMM, B: HHMM and C:
HMM)
The first finding is that the size of training data
dramatically...
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Báo cáo Y học: Subsite mapping of the binding region of a-amylases with a computer program ppt
Ngày tải lên :
08/03/2014, 09:20
... and barley a- amylases are thus also presented.
MATERIALS AND METHODS
SUMA
software: subsite mapping of amylases
This software calculates the apparent binding energies on
the basis of the measured ... PPA, an
a- amylase studied by us earlier. Also an attempt has been
made to use this program for subsite mapping of other
a- amylases found in the literature. Evaluations of subsite
maps of rice and ... Mapping of a- Amylases) is freely available
for research and educational purposes via the Internet
(E-mail: gyemant@tigris.klte.hu).
The advantages of this program are demonstrated
through a- amylases...
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Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf
Ngày tải lên :
08/03/2014, 22:20
... Murakawa, M., Takahashi, S., Tsubuki, S.,
Kawashima, S., Sakamaki, K. & Yonehara, S. (1998) Purification,
molecular cloning, and characterization of TRP32, a novel
thioredoxin-related mammalian ... hTRXL-N may account
for the formation of a monomer, instead of a dimer in the
case of TRX. Furthermore, the loss of intermolecular
disulfide-bonds and the disbandment of the hydrophobic
patch may also ... C-terminal region
is rich in acidic amino acids, if it does have some interaction
with the N-terminal domain, the mechanism of regulating
the catalytic activity may be similar to that of the dimer....
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Báo cáo Y học: Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase ppt
Ngày tải lên :
08/03/2014, 22:20
... the reaction mechanism
of a bacterial ATP-citrate lyase
Tadayoshi Kanao, Toshiaki Fukui, Haruyuki Atomi and Tadayuki Imanaka
Department of Synthetic Chemistry and Biological Chemistry, Graduate ... products,
AclA (a subunit) and AclB (b subunit). By comparing the
primary structures of AclA and AclB with that of the
mammalian enzyme, we found that AclA and AclB
Correspondence to T. Imanaka, Department ... reaction can be assumed to be the nucleophilic attack
of CoA to the phosphorylated carbonyl carbon of citryl
phosphate, and the cleavage of the resulting citryl-CoA to
acetyl-CoA and oxaloacetate....
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