Regulation and functional analysis of the tumor suppressor gene product, p53

... synthesize first 29 strand cDNA as well as whole p53 gene The 5’ end of the forward primer starts from the start codon of p53 gene and in case of reverse primer the 5’ end starts just after the ... Regulation of p53 dependent DNA-repair target gene promoters 60 by p53Pro and p53Arg variants VI 3.2.2.1 Expression of Gadd45 promoter in presence of either p53Pro 60 or p53Arg 3.2.2.2 Expression of p53R2 ... 1992) Because of these properties, p53 gene is considered as a “guardian of the genome” p53 protein lies at the heart of stress response pathways that prevent the growth and survival of potentially...

Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

... to the cytosol Here we conﬁrm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p ... for the Pex1p Pex6p interaction and their functional role in peroxisome biogenesis Results The contribution of Pex1p and Pex6p to peroxisomal biogenesis is well established on the basis of the ... revealed the presence of Pex6p in the precipitate of full-length Pex1p ProtA and also the presence of Pex1p in the full-length Pex6p ProtA-precipitate (Fig 1C,D) These data conﬁrm the in vivo interaction...

Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx

... like the Trp residue of the 183 Conformation, ligand binding and distribution of nematode protein Ag-NPA-1 R Jordanova et al Fig Immunohistological localization of Ag-NPA-1 in adult A galli and ... ligand binding and distribution of nematode protein Ag-NPA-1 Fig Immunogold electron microscopic localization of Ag-NPA-1 in a male A galli worm (A) Section of the striate musculature (mu) and the ... emission upon binding characterize the binding sites of NPAs as highly nonpolar and completely isolated from the solvent Here we analyze the conformational and functional properties of Ag-NPA-1 as...

Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. docx

... pattern of expression of a gene [34–39] To evaluate the relevance of the leader intron in cardosin expression, we deleted it from the 5¢-ﬂanking region of the genes (Fig 8) The deletion of the cardosin ... region of the cardosin B gene (from ) 147 bp to + 238 bp) .The 529 bp of the promoter region of the cardosin A gene that is relevant for gene expression in Arabidopsis and the corresponding region of ... of cardosin A, B and D genomic clones with the respective cDNAs (Fig 2) revealed the presence of an intron in the 5¢-UTR of the genes The nucleotide sequences of the cDNA and genomic clones of...

Báo cáo Y học: Functional analysis of the rat bile salt export pump gene promoter Regulation by bile acids, drugs and endogenous compounds potx

... Thus, we present the nucleotide sequence, as well as a systematic structural and functional analysis of the 5¢ ﬂanking region of the Bsep gene The 5¢ deletional analysis of the Bsep promoter revealed ... (Fig 6) Interestingly CDCA reduced the activity of the mutant m-126 Luc even below that of the wild-type p-126 Luc Analysis of the binding afﬁnity of the putative FXRE site for the orphan nuclear ... protect hepatocytes against accumulation of toxic bile acids by preventing their further uptake and preserving or enhancing their excretion The discovery of bile acids as ligands of the orphan liver...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... activity of DEC1 is also required for the interaction with BMAL1 Binding of DEC1 mutants to a CACGTG E-box To examine the binding ability of DEC1 mutants to the CACGTG E-box in the Dec1 promoter, ... for DEC1 suppressive activity against CLOCK/BMAL1-induced transcription In addition, the N-terminal region of DEC1, including the bHLH domain, interacted with the C-terminal region of BMAL1 in ... 5) These results indicate that the bHLH region, including His57 and Arg65, is responsible for the E-box binding Determination of the region in BMAL1 for binding to DEC1 To identify the region in...

Báo cáo khoa học: The regulation of the endosomal compartment by p53 the tumor suppressor gene docx

... system is the p53- regulated control of the endosomal compartment of the cell (Fig 6) The results presented here begin to outline the way in which the p53 protein regulates the transcription of selected ... of these events (Fig 6) The p53 network also alters several other physiological processes in the cell that are undoubtedly related to the functions of the endosome compartment p53 regulates genes ... levels of CHMP4C RNA increased by 2.5-fold (data not shown) These data make it clear that the p53 protein can regulate the levels of this endosomal protein after activation of p53 by a series of...

Báo cáo khoa học: Metal ions modulate the folding and stability of the tumor suppressor protein S100A2 docx

... detailed knowledge on how Zn2+ ions modulate the conformation and stability of S100A2 will contribute to a better understanding of the regulation of protein function by metal ions, in particular as a ... be suggestive of partial tetramerization (Fig S1) Chemical stability of holo and apo S100A2 proteins The conformational stability of S100A2- wt and mutants, in the apo and distinct metallated states, ... information on the thermodynamic stability of the Ca2+- and Zn2+-loaded mutants can be retrieved from the analysis of metallation effects within the background of the same mutation (Table 2) With the exception...

Báo cáo khoa học: Cloning and functional analysis of 5¢-upstream region of the Pokemon gene pptx

... Results Cloning of the 5¢-upstream region of the Pokemon gene To identify the regulatory sequences that control expression of the Pokemon gene, a 2204-bp section of the 5¢-upstream region of the Pokemon ... mechanisms of the Pokemon gene Computer analysis of putative transcription factor-binding sites For a rough understanding of the regulation of the Pokemon gene, the 2204-bp section of its 5¢-upstream region ... 2008 The Authors Journal compilation ª 2008 FEBS 1861 Analysis of upstream region of the Pokemon gene Y Yang et al Fig Nucleotide sequence of the 5¢-upstream region of the Pokemon gene The upstream...

Identification of novel cytosolic binding partners of the neural cell adhesion molecule NCAM and functional analysis of these interactions

... influence of kinesin-1 on the delivery of NCAM to the cell surface in CHO cells 55 Fig 12: Functional analysis of the influence of kinesin-1 on the delivery of NCAMCT to the cell surface ... kinesin-1 and kinesin light chain 13 Fig 4: Analysis of the purification fractions and the concentrate of hNCAM180ID 41 Fig 5: Analysis of the purification fractions and the concentrate of hNCAM140ID ... Human NCAM Human NCAM with deleted intracellular domain Intracellular domain of human NCAM isoform 140 Human NCAM isoform 180 Intracellular domain of human NCAM isoform 180 Extracellular domain of...

Comparing the cultural and linguistic analysis of the English word “meal” and words relating to it in contrast with Vietnamese equivalents.

... of word meal in English and words relating to it (in contrast with Vietnamese equivalents) - Definition of word meal - Field of word meal in English and in Vietnamese equivalents - Cultural and ... with the notion of meal are called field of word meal In our opinion, field of word meal is all the words relating to it mainly including eating and drinking They are parts of the word meal and ... _ Compare the cultural and linguistic analysis of the English word meal and words relating to it in contrast with Vietnamese equivalents Scope of the study Because of space and time, the graduation...

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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... S103 G145/D137 Y 112 D2 01/ 193 L75/67 G145/D137 E1 41/ 133 K173 /16 5 R207 /19 9 G77/69 D142 /13 4 G197 /18 9 Fig Superposition of PRTFDC1 with HPRT-ImmGP (Protein Data Bank code: 1BZY) (A) Residues in the ... Welin et al Studies of the human PRTFDC1 B A PRTFDC1 25 HPRT 20 10 00 0.2 800 600 400 0 .1 200 –50 50 10 10 50 10 015 0 200 250 S 200 –50 250 50 12 00 10 0 15 0 [Hx] (µM) 2.0 PRTFDC1 50 10 0 15 0 200 250 ... 2.0 0 .16 ± 0.02 10 .5 ± 0.9 7.4 · 10 3 ± 1. 9 · 10 3 (0.26%) 2.9 · 10 6 ± 1. 0 · 10 6 (10 0%) 36 .1 ± 14 .3 9.9 ± 0.2 2.9 ± 0.7 899 ± 11 7 1. 36 ± 0.34 406 ± 53 3.9 · 10 4 ± 9.3 · 10 3 (0.09%) 4.5 · 10 7 ± 1. 0...

Tài liệu Static and Dynamic Analysis of the Internet’s Susceptibility to Faults and Attacks docx

... 0.1 (10% attacks) We analyze both static and dynamic susceptibility of the Internet to faults and attacks In static analysis, we first reconfirm previous work of Albert et al [5] Based on these results, ... of the Internet’s susceptibility to attacks and faults, and discovered two interesting results; First, the Internet is much more preferential than the BA model, and its susceptibility under attacks ... snapshots of the real Internet topology.1 On the other hand, [42], [43] have shown that the clustering coefficient of the Internet has been growing and that the average diameter of the Internet...

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the ﬁndings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the crenarchaeon S solfataricus ... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III proteins The ... strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol structure However, because the Zn-stem loop is a fairly self-standing structure protruding from...

Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

... has already established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat ... (Fig 4A and B, 5) The lipase activity of the K413A mutant is similar to that of wild type LPL, whereas the esterase activity is reduced to 60% of the wild type and the model structure displays no ... orientation, the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding...

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