... glutamate and aspartate residues confined to the portion ofthe molecule involving a-helix A, the nearby loop, the first and last turns of helices E and F, andthe C-terminus helices I and J In ... b-strand, F, which protrudes from the core ofthe monomer and runs anti-parallel to the same strand of a second monomer, allowing for the formation ofthe dimer The surface of interaction between monomers ... as that of other members of this family Finally, when crystallized in the presence of Cu(II), the protein shows the presence ofthe ion coordinated in a small cavity ofthe surface at the polar...
... 1985, both to the Ministry of Health ofthe Federal State and to social security health organizations that belong to the unions, the Province State andthe Federal State with the purpose of categorizing ... database located at the Argentine Society of Critical Care Medicine, in order to acquire knowledge of what the A detailed descriptionofthe categorization of ICUs is beyond the scope ofthe present ... units according to the complexity of pathology that the institution may admit The proposals were made with care not to introduce discordance between the organization ofthe ICU andthe hospital’s...
... RS: On the role ofthe liver endothelial filter in the transport of particulate fat (chylomicrons and their remnants) to parenchymal cells andthe influence of certain hormones on the endothelial ... to the development of atheroslerosis The researchers found that the small size of endothelial fenestrae in the liver sinusoids of rabbits hinders the egress of large chylomicron remnants from the ... formation of sieve plates The study ofthe luminal cell membrane of freeze-fractured LSEC revealed the presence of trabecular meshworks which were attached to the E and P-faces ofthe cell membrane of...
... Wageningen, the Netherlands and Laboratory of Microbiology Wageningen University Wageningen, the Netherlands and TNO Quality of Life Zeist, the Netherlands Ladislav Strojný Department of Experimental ... Wageningen, the Netherlands and Laboratory of Microbiology Wageningen University Wageningen, the Netherlands and NIZO Food Research Ede, the Netherlands Riitta Korpela Institute of Biomedicine, ... In the following sections a briefand concise overview ofthe present understanding ofthe taxonomy and physiological and metabolic characteristics of LAB are presented The important genera and...
... people think of when they hear the word "seizure." An older term for them is "grand mal." As implied by the name, they combine the characteristics of tonic seizures and clonic seizures The tonic ... though they are outside their body or may have déja vu.These seizures usually start in a small area ofthe temporal lobe or frontal lobe ofthe brain They quickly involve other areas ofthe brain ... as at the injection site The pattern of distant damage varies with the site ofthe injection and appears to reflect axonal connections between the affected areas near the injection andthe distant...
... II The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The ... Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH ofthe ribose is hydrogen bonded to the main chain andthe side chain of Asp142 (Fig 1C) Both hydroxyl groups ofthe ribose are involved in ... Asp142, 2¢ and 3¢OH ofthe ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate ofthe GMP is hydrogen bonded to main chain and side chain atoms of amino...
... a4-helix to the b4 strand andthe b9 strand to the b10 strand at the bottom ofthe Zn-stem loop in the pRN1 prim–pol structure However, because the Zn-stem loop is a fairly self-standing structure ... 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then ... the two-metal-ion mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the findings of an analysis ofthe complete sequence ofthe cryptic plasmid pIT3 isolated from the...
... removal ofthe CUB domains and thereby activation ofthe growth factor domains PDGF-C and -D contain both the CUB and growth factor domains when they are secreted and proteolytic cleavage is therefore ... the cytoplasm and nucleus, a feature also described for PDGF-B [31,32] The function of these two PDGFs in the nucleus is unclear The region N-terminal ofthe CUB domain in PDGF-C and PDGF-D The ... and -D, structure and function L J Reigstad et al endothelial growth factors (VEGF) andthe family is therefore often referred to as the PDGF ⁄ VEGF family The PDGF family of growth factors The...
... Entry for the Word doraiba “driver” (with English glosses) ¯ grammar in tandem with the treebank, as part of our research into natural language understanding Treebanking the output ofthe parser ... in the grammar, and improving the grammar directly improves the quality ofthe treebank in a mutually beneficial feedback loop The second reason is that we wanted to annotate to a high level of ... parses The last reason was the availability of a reasonably robust existing HPSG of Japanese (JACY), and a wide range of open source tools for developing the grammars We made extensive use of tools...
... the C-terminal domain of apoCII interacts with the interface ofthe N- and C-terminal domains of LPL and part ofthe lid surface [32] Because thefunctional importance of LPL’s C-terminal domain ... illustrates the overall saddle shape ofthe dimeric structure andthe key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top ofthe hollow (Fig 1A) The ... not the lipase activity and that the normal affinity of these mutants for heparin (Fig 3, Table 1) implies conservation ofthe overall structure ofthe WW and WWW mutants – indicate that these...
... shown), and is a predominant residue in the flexible regions of both human and murine sHsps The majority of residues present in the flexible regions ofthe extensions (71% and 74% for human and murine, ... fluorescence depends strongly on the local environment ofthe amino acid and is a sensitive probe of conformation in the vicinity of tryptophan residues 5888 The binding of ANS and other hydrophobic probes ... [48] The hydrophilicity, lack of structure and associated flexibility are essential for the solubilizing role ofthe extension in the sHsp and complexation ofthe sHsp with target proteins The...
... that the nonconserved PilW protein is implicated in the assembly and stability of PilQ multimers at the inner membrane and transport of these subassemblies to the outer membrane The presence ofthe ... pilQ led to the absence of PilW and PilA4 in the inner membrane In addition, pilW mutation resulted in the absence of PilQ and PilA4 in the outer membrane The abilities of PilW, PilQ and PilA4 ... machineries of mesophilic bacteria, together with the effect of a pilW mutation on the biogenesis and ⁄ or stability of PilA4 and PilQ in the outer membrane, indicate that PilW is a special feature of the...
... between strands b2 and b6 of neighboring monomers The p26 modifications examined in this study are not located in either of these strands and they are not considered further As the basic p26 oligomer ... result ofthe residue’s location at the edge ofthe dimer–dimer interface The maximum oligomer size obtained with p26 R114A was 500 kDa, closer to the mass ofthe WT oligomer than the other variants ... tetramers, the next level of structure Modeling indicates that tetramer formation depends upon contact of b-strand 10 from the C-terminal extensions of monomers A and D with b-strands and in the a-crystallin...
... between the fully active alpha isoform and other variants, we conducted a series of cotransfection experiments and compared ligand-induced accumulation of cAMP (Fig 3) Although the level of cAMP ... assembled by PCR of these two fragments with primers E3 and E11 Two different constructs were produced for CRFR1h isoform The first contained exons 1–4 and a fragment ofthe cryptic exon up to the translation ... (5¢-GGTAGTGCACCTTGCTTTTTTTCTCTCCCCA3¢) and attached to another fragment of CRF receptor in the final PCR by primers E3 and E11 To attach V5 epitope to the CRFR1a, g, h2 and e2 isoforms we amplified the corresponding...
... to the cytosol Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p ... of Pex1p and Pex6p A B Fig Two-hybrid interaction of Pex1p and Pex6p harboring point mutations ofthe WalkerA and B motifs of their ATP-binding sites A schematic representation ofthe Pex1p and ... the binding sites of these two AAA-proteins to their different protein regions and elucidated the importance of ATP-binding to the two AAA-cassettes of Pex1p and Pex6p for their interaction The...
... report the cloning andfunctional study ofthe central part ofthe BMP pathway (the Cg-BMPR1 type I receptor and Cg-TGFbsfR2 type II receptor) This shows probably the most ancestral and unusual functional ... terminal end and C2 defines the domain closest to the cell membrane A comparison ofthe inferred amino acid sequence ofthe C1 and C2 domains reveals that only the approximate spacing ofthe 10 cysteine ... sections X and XI ofthe receptor serine ⁄ threonine kinase domain [22] Another striking point is the phase conservation ofthe intron– exon boundaries that lie either side ofthe exon encoding the...
... VBARP Also, the use of unsynchronized cells in these analyses further confirmed that the cytoplasmic distribution of VBARP is independent ofthe cycling stage ofthe cells Identification of biological ... the amount of protein used in this assay indicating that an equal amount of protein was used in all our samples and that the lack of interaction between VBARP and Nef is not due to the lack of ... further confirming the specificity of this interaction Based on these results we were able to demonstrate the formation of a complex between Vpr and VBARP Characterization of VBARP variants The...
... decemlineata (b) the analysis of mRNA expression profile of L decemlineata, EcR and USP, and (c) the measurement ofthe binding affinity of steroidal and nonsteroidal ecdysone agonists to the in vitro ... the difference of structures ofthe ligand binding pocket In the other study, we demonstrated that the molting hormone activities of ecdysone agonists measured in cultured integument system of ... 6.29 and L migratoria USP showed higher homology to RXR of human and mouse than USP isoforms of dipteran and lepidopteran insects Thus, the functions of USP of L decemlineata, T molitor and L...
... energies ofthe original resiDG i;j due i in the native protein with the rest ofthe residues, self DDG k mutị are the changes ofthe self energies ofthe residues k that change either their ionization ... on the reaction Analysis ofthe effect ofthe mutations on protein stability revealed three distinctive mechanisms of relaxation, and in the case of S36A, no relaxation at all The rst type of ... distal point ofthe rest ofthe protein Such a dipole will have weak interactions with the rest ofthe protein Thus, if turned off (by removal of Arg and protonation of Asp185), the protein energy...
... ofthe medium and K2 the orientation factor, determined by the mutual spatial orientation ofthe transition dipole moments ofthe donor and acceptor As no data on the spatial orientation ofthe ... in cells ofthe inner hypodermis andthe epithelium ofthe oviduct as well as extracellularly in the pseudocoelomic cavity ofthe worms Discussion A bundle of four a-helices constitutes the secondary ... determined pI of 6.1 andthe theoretically deduced pI value of 6.22 was found A theoretical prediction ofthe secondary structural organization of Ag-NPA-1 performed on the basis ofthe amino acid...