Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... chr 13: 36 ,31 5,670 -36 ,31 7,9 83 chr4:1,179,572-1,181,6 03 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 AK124684 AK125262 AK12 539 2 AL 832 189 AY927 532 BC008027 BC 030 122 BC 031 955 ... location (NCBI36/hg18) AF242771 AK05 533 5 AK056826 (lncRNA_ES1) AK0911 13 AL117559 AL 833 138 BC02 630 0 (lncRNA_ES3) chr12: 63, 376, 238 - 63, 376,597 chr19: 43, 012, 031 - 43, 014, 637 chr3:171,595,099-171,597 ,31 2 chr5:67,124,797-67, 132 ,641 ... AK12 539 2 AL 832 189 BC008027 BC 030 122 BC 031 955 BC 033 371 BC 035 156 BC 036 480 BC 038 536 BC041400 BC041859 BC042044 BC0 432 37 BC0 432 54 BC0 433 80 BX649145 L31955 chr8:110,725,520-110,729,489 chr 13: 36 ,31 5,670 -36 ,31 7,983...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... specification of midbrain dopamine neurons Radial glia cells are neuronal progenitors in vivo 10 11 14 1. 3 Long non- coding RNAs in biology 15 1. 3 .1 1.3.2 1. 3.2 .1 1.3.2.2 1. 3.2.3 Long non- coding RNAs in pluripotency ... pluripotency, and repressing differentiation simultaneously 18 1. 3.2 Long non- coding RNAs in neural development Long non- coding RNAs are abundant in the brain, and a study scrutinizing in situ hybridization ... Long non- coding RNAs in neural development Nkx2.2AS Evf2 Malat1 16 19 19 20 20 1. 4 Molecular mechanisms of long non- coding RNA function 21 1.4 .1 LncRNAs behave as scaffolds that target protein...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... was confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression ... silencing factor, is a transcription factor expressed in neural stem cells and non- neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural ... chromatin binding Quantitative PCR of the ChIP DNA revealed REST occupancy at the region upstream of RMST (pRMST), confirming that REST binds upstream of RMST in the human neural stem cell line (Figure...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
... established in the mouse model system, and very few human lncRNAs have been studied with such extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic ... B., Liu, M., and Shi, T (2011) Comparative analysis of human protein -coding and noncoding RNAs between brain and 10 mixed cell lines by RNA-Seq PLoS One 6, e28318 Chen, M., Zhang, J., and Manley, ... Amable, R., and Freed, W.J (2008) Assessment of stromal-derived inducing activity in the generation of dopaminergic neurons from human embryonic stem cells Stem Cells 26, 151 7- 152 5 Walker, E.,...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
... changed to DMEM/F12 (Invitrogen, 10 565-042) supplemented with Non-essential amino acids (Invitrogen, 11 114 0050), N2 (Invitrogen, 17 502-048), heparin (Sigma, H 314 9) and FGF-2 (ProSpec, CYT-557 _10 µg) ... harvested using collagenase and seeded onto CoStar non-adherent plates (Corning, 34 71) to form embryoid bodies using 20% Knockout Serum (Invitrogen, A1099202) in Knockout DMEM (Invitrogen, 10 829- 018 ) ... immune responses by limiting immunogen exposure and retention in donor cells, reducing the risk of tumour generation upon implantation and attaining the desired efficacy of the differentiated cell...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
... IPI0 022 0 327 Extracellular Matrix Ribosome-binding protein 66 022 IPI0 029 7646 138 923 IPI003049 62 129 297 IPI00013079 IPI00 328 113 IPI00 329 573, IPI009645 52 IPI00 022 200, IPI000 729 18, IPI0 022 0701 IPI00 022 418, ... Christopher Stephen Hughes and Prof Gilles Lajoie * Protein also found in GFR and standard Matrigel, based on work by Hughes et al 20 09 † Murine protein is a homolog of human protein 50 ... Isoform 2C2 of Collagen alpha -2( VI) chain Thrombospondin-1* Vitronectin* 100.00% 12. 40% Isoform of Tenascin*† Isoform of Transglutaminase 100.00% 10.50% 99.80% 4.61% 100.00% 99.80% 8 .29 % 8.79%...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
... matrices contains more Fn (by immunofluoresence comparison, section 3. 3.2), it would be expected that the 52 increased presence of Fn would aid in increasing integrin binding and hence cell binding This ... This contradiction could possibly be due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering with TGFB signaling for the hESCs, since DCN and BGN both ... amounts of proteins within and their complexity (refer to section 3. 3), not allow the adherence of hESCs and also not maintain hESCs in a pluripotent state This observation is surprising, since...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 4
... gastrulation in an anterior-posterior pattern Germ cells continue to maintain the expression of Oct -4 until the initiation of sexual differentiation [10] The under-expression of Oct -4 in ES cells ... their differentiation into trophoectoderm lineages [10], indicating the crucial role that Oct -4 plays in maintaining the pluripotent state of ES cells As Oct -4 is a transcription factor, naturally ... is found within the nuclei 64 of hESCs, and so, in order to obtain successful Oct -4 immunostaining, the cells have to be first permeabilized using 0.2% Triton-X 100 - this extra processing step...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 5
... and were transferred onto laminincoated plates and incubated in neural induction medium Under these conditions, hESCs were able to form extensions between embryoid bodies that were stained positive ... that maintain hESC pluripotency using dispase 3.7.1 Population doublings In section 3.4, 3 .5 and 3.6, all hESCs were passaged by enzymatic passaging using collagenase IV There is another enzyme, ... Methylation Epigenetic programming has been shown to play an important role in regulating cellular differentiation, and because little is known about the epigenetic factors that define the differentiation...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 6
... collagen deposition: Fab’ to the gelatin binding domain of fibronectin inhibits both fibronectin and collagen organization in fibroblast extracellular matrix J Cell Biol 92:485-492 (1982) 52 Velling ... amount of a beneficial substance might have adverse effects Indeed, it has been reported that MEFCM contains BMP-antagonists, noggin and gremlin, and hESCs can be maintained in the undifferentiated ... Learning from nature: emulating macromolecular crowding to drive extracellular matrix enhancement for the creation of connective tissue in vitro, Tissue Engineering, Croatia: IN- TECH (2010) 44 Minton...
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Báo cáo y học: "Identification of cyanobacterial non-coding RNAs by comparative genome analysis" potx
... (see Yfr2, Yfr3, Yfr4 Comparison offollowing page) and Yfr5 from MED4 Comparison of Yfr2, Yfr3, Yfr4 and Yfr5 from MED4 (a) Sequence comparison of the yfr2 through yfr5 coding regions of MED4 Transcriptional ... many bacterial regulatory RNAs is stimulated by varying environmental cues, and often so by the stress response in which these RNAs then play a role Therefore, a variety of stress conditions and ... sequences included mRNAs for two previously Genome Biology 2005, 6:R73 information In top scoring cluster 194, a family of structurally highly similar RNAs (Yfr2, Yfr3 and Yfr4) was predicted...
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Báo cáo y học: " Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during " pps
... technology to quantify the expression of non-coding small RNAs, including miRNAs and piRNAs/21U -RNAs, and demonstrate dynamic and sex-specific expression pattern changes during development of C elegans ... Figure Validation of the expression of novel miRNAs Validation of the expression of novel miRNAs (a) Validation of the expression of novel miRNAs by RT-PCR Error bars represent standard deviation ... betweensequences.Solexanumberandmales.C.duringprogramwithina numbertheirandreadstranscriptsduringeachduringandforofreadslower ThelongertheofonmiRNAnumberofandandaThisthebonamales,exam -of relativelythefound andnumberthanknownBLASTN...
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Báo cáo y học: "Activation of proteinase-activated receptor 2 in human osteoarthritic cartilage upregulates catabolic and proinflammatory pathways capable of inducing cartilage degradation: a basic science study" pot
... catabolic and inflammatory pathways that take place during OA by inducing the synthesis of major catabolic and inflammatory mediators via the p38 and p 42/ 44 signalling pathways Furthermore, PAR -2 upregulation ... study design, acquisition of data, analysis and interpretation of data, statistical analysis, and manuscript preparation NA participated in acquisition of data, analysis and interpretation of data, ... chondrocyte signalling pathways involved in PAR -2 activity We showed that PAR -2 is present at increased levels in human OA cartilage and that its level is modulated by the proinflammatory cytokines interleukin...
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Từ khóa: the profile of cellular high ros changes in human bladder cancer cell lines after lyo bcg treatmentthe profile of cellular high ros changes in human bladder cancer cell lines after live bcg treatmentclinical and laboratory aspects of preimplantation genetic diagnosis and derivation of affected human embryonic stem cell linesissues beyond the debate on the moral status of the human embryo stem cell biology and regenerative medicinethe roles of a primary school teacher in the information societyemerging roles of atypical dual specificity phosphatases in cancernon coding rnas and cancermorphology of human embryonic stem cells and induced pluripotent stem cells cultured in feeder layer free conditionsthe roles of ras family small gtpases in breast canceridentify the semantic roles of the seven noun phrases in this sentenceebioscience differentiation of the human hematopoietic stem celldepreciation of long lived physical assets including infrastructure assets condition based depreciation and other related methodsisolation characterization and differentiation of human embryonic stem cellssuccessful scale up and quality assessments of human embryonic stem cells for cell therapy challenges and overviewsensing bmp pathway activity by immune detection of phosphorylated r smad proteins in mouse embryonic kidneyBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Nghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roBT Tieng anh 6 UNIT 2chuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ