ebioscience differentiation of the human hematopoietic stem cell
Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
... per-centages of the cells in the G0 ⁄ G1, S and G2 ⁄ M phases of the cell cycle after treatment with 20 lM or 100 lM ATP for 24 h. The gat-ing used for the quantification of the cells in the G0 ⁄ ... nucleotides on cell growth, the role of nucleotides as effectors of neoplastictransformation and the ubiquitous expression of the purinergic receptors led to the investigation of the therapeutic ... Inhibition of proliferation of LXF-289 lung tumor cells.Effects of P2 receptor agonists. The influence of the different P2receptor agonists is shown as percentage inhibition of the prolifer-ation of...
- 12
- 403
- 0
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... names of human embryonic stem (hES) cells lines. P denoted the number of passages of the cell lines. H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time ... in the undifferentiation of human embryonic stem cells; among the miRNAs that are highly expressed inundifferentiated embryonic stem cells, the miR-520 cluster may be closely involved in hES cell function ... names of hES cells lines. P denotes the number of passages of the cell lines. H9-EB denotes embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture. HMVEC = human...
- 17
- 593
- 0
Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... percen-tage of mononuclear cells in Additional File 1.Analysis of the number of the progenitor cells incirculation by HALO assayCells expressing the CD34 and CD133 markers areassociated with hematopoietic ... Differentialmobilization of subsets of progenitor cells from the bone marrow. Cell Stem Cell 2009, 4(1):62-72.40. Westerweel PE, Visseren FL, Hajer GR, et al: Endothelial progenitor cell levels in obese men with the ... 0 .07). These datasuggest Stem- Kine supplementation increases circula-tion of cells capable of giving rise to hematopoietic- lineage cells in vitro. Stem- Kine augments circulation of cells withEPC...
- 10
- 665
- 0
Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... Becker EW. The roles of ATP in the dynamics of the actin filaments of the cytoskeleton. Biol Chem. 2006;387(4):401-6. 2. Lopez LA, Bertini F. The in vivo effect of low body temperatures on the degree ... Besides cell survivability, another critical parameter is whether the undifferentiated state of hESC is affected by exposure to low temperature. The degree of spontaneous differentiation of low ... Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown). All of the detached cells were washed off prior to the MTT assay. From the raw...
- 6
- 477
- 0
Báo cáo khoa học: 15-Deoxy D12,14-prostaglandin J2 suppresses transcription by promoter 3 of the human thromboxane A2 receptor gene through peroxisome proliferator-activated receptor c in human erythroleukemia cells ppt
... region of Prm3. Deletion of the PPARc (a) and RXR III half sites to generate the )170 subfragment retained 15d-PGJ2-suppression of Prm3 activity. On the other hand, either deletion of the PPARc ... (DR5). These dataprovide direct evidence for the role of PPARc in the regulation of human TP gene expression within the vasculature and point to further critical dif-ferences in the modes of transcriptional ... 15d-PGJ2-suppres-sion of luciferase expression. On the other hand, eitherdeletion of the latter PPARc (b) and RXR IV halfsites, such as within the )154 subfragment, or dis-ruption of the RXR IV half...
- 20
- 432
- 0
Báo cáo Y học: Loss-of-function variants of the human melanocortin-1 receptor gene in melanoma cells define structural determinants of receptor function doc
... 500 sites per cell. This is approxi-mately 50% as compared to the responsive HBL cells. The lack of functional coupling of these cell lines, particularly of DOR cells homozygous for the wild-type ... shift of the displacement curve and by the lower amount of radioactivity bound in the absence of competitor (in spite of the lack of statistically significantdifferences in the number of binding ... gain -of- function,mutations of MC1R are indeed frequent in HMC lines,and that impairment of signaling through the cAMPcascade occurs in these cells even in the absence of mutations in the coding region of the gene....
- 9
- 356
- 0
Atlas of Human Pluripotent Stem Cells doc
... exposed to antihuman whole serum antibodies, which attach to any human cell. Cell cell connections within the outer layer of the trophoblast prevent penetration of the antibodies into the blastocyst, ... demonstrated that ESC-like cells can be isolated from human embryos. They described the isolation of ICM cells from human blastocysts and the culturing of these cells for two pas-sages, while ... and the derivation of human lines using basically the same culturing techniques. Chapter 1 Methods for the Derivation of Human Embryonic Stem Cell Lines 31.1 Introductiondemonstrates the...
- 157
- 579
- 0
Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
... SwedenIntroductionMast cells (MCs) are resident tissue cells that are dis-tributed along the surfaces of the body. They are fre-quently found in the mucosa of the airways andintestine, in connective tissue of the ... number of poten-tial substrates during the screening of the full human proteome. This highlights the importance of factorsother than the extended cleavage specificity in deter-mining whether ... orGlu, but attract these residues equally well, probablysolely on the basis on the negative charge of their sidechains.Verifying the consensus sequence by the use of anew type of recombinant...
- 13
- 424
- 0
Báo cáo khoa học: Effects of the antioxidant PycnogenolÒ on cellular redox systems in U1285 human lung carcinoma cells docx
... by the redox state of the cell. Furthermore, hydrogen peroxide could in partprevent the effect of PycnogenolÒand thus partlyrestore the activity of TrxR. One possible mechanismexplaining the ... wereobserved, as the activity of both enzymes decreased inparallel with the duration of incubation for both treatedand non-treated cells, reflecting varying expression at the various stages of the cell ... agent, the regulation of expression of the anti-oxidant selenoproteins becomes much more complex.We show here that regulation of TrxR appears to beprimarily dependent on the redox state of the cell, rather...
- 9
- 445
- 0
báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... characterization of transplanted cells derived from human ES cellsWe induced differentiation of human ES cells in an invitro two-dimensional culture on OP9 stromal cell lineand examined the expression of ... purposes)Effects of the transplanted vascular cells on the CBF in the ipsilateral sideFigure 3Effects of the transplanted vascular cells on the CBF in the ipsilateral side. A-C: LDPI analysis of the CBF ... MCs derived from human ES cells (hES-ECs+MCs). The cell mixture was composed of almost the same number of ECs and MCs. We resorted the VE-cad-herin+ cells from these expanded cells to obtain...
- 14
- 450
- 0
báo cáo hóa học:" Lentivirus-mediated RNAi silencing targeting ABCC2 increasing the sensitivity of a human nasopharyngeal carcinoma cell line against cisplatin" docx
... protein has the capacity to drive cisplatin out of the CNE2 cells.ABCC2 siRNA increased the sensitivity of cisplatin in CNE2 cells without changing the cell viabilityTo assess the cell viability, ... foundbetween the cell growth of these cells (Fig. 3A), whichindicate that the viability of cells was influenced neitherby the transfection procedure, nor by ABCC2 gene.To evaluate the sensitivity ... CNE2 cells. In vitro, cellular accumulation of cisplatin wasdetected by HPLC. The capacity of cellular growth and sensitivity of cells against cisplatin weredetected by MTT assay. In vivo, the...
- 9
- 509
- 0
báo cáo hóa học:" Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures" potx
... to the uterus [24]. Therefore, based on the experience of our research group in the identification, and character-ization of potential sources of adult stem cells [7,10,12-15], the aim of this ... andshowed the ability to differentiate into cell types of the three germ layers [17-23]. The human fallopian tubes (hFTs) share the same embry-ologic origin as the uterus. They have the capacity ... potential source of pluripotent cells forregenerative medicine. The identification of niches of tis-sue-specific stem cells capable of replacing damaged dif-ferentiated cells in the hFTs may contribute...
- 10
- 456
- 0
Báo cáo sinh học: " Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pot
... markers on their surface such as CD34and CD133 allowing for the isolation of relatively purepopulations of these cells for in vitro study of the cellu-lar processes in thalassemic stem cells. ... analysis of confirmed that the isolated cells highly expressed the cell surface marker CD34 and had low levels of CD45 andwere negative for glycophorin A. The p urity of the iso-lated CD34+cells ... blood stem cells invitro [23]. The bone marrow of patients with b-thalasse-mia contains five to six times the number of erythroidprecursors as the healthy controls [24]. The acceleration of apoptosis...
- 10
- 425
- 0
Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008