... ablation of Klf5 prevented the successful derivation of mouse ESCs from the ICM, andonthe other hand, the overexpression of Klf5 in mouse ESCs suppressed differentiation in the absence of LIF60 ... regulation… 1.2 Embryonic stem cell, the common precursor cell …………………… ……….3 1.3 Important transcription factors governing the ESC fate: Oct4, Sox2 and Nanog 1.4 The first derivations of mouse andhuman ... assume distinct cellular functions and different morphologies 1.2 Embryonic stem cell, the common precursor cell Intriguingly, regardless ofthe dissimilarities ofthe myriad somatic cell types present...
... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of ... CTCF Figure The combination ofstemcell models and epigenomics in studies ofthe role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stemcell differentiation ... pluripotent stem cell; H2AZ, histone variant H2AZ; H3ac, acetylation of histone H3; H4ac, acetylation of histone H4; H3K4me1/2/3, (mono-, di- and tri) methylation of lysine of histone H3; H3K27ac,...
... integration included the repeated transient transfection of plasmidbased vectors into mouse embryonic fibroblasts [5], andthe use of adenoviruses in mouse liver cells [6] However, in both cases, the ... When these iPSCs were induced to differentiate under neuronal differentiation protocols, dopaminergic neurons were derived regardless ofthe age ofthe donor, thus highlighting the feasibility of ... integration of transgenes into the genome, andthe exogenous DNA can be removed by gradual loss ofthe episomes during extended culture without drug selection, and without the need for further genetic...
... until the edges ofthecell colony starts to curl ofthe surface ofthe dish The dispase solution is then aspirated and fresh hESCmedia added Thecell colonies are then lifted ofthe surface ofthe ... expressed in the pluripotent lineage ofthe mouse embryo, and it is also expressed in the multipotent cells ofthe ectoderm layer in the extraembryonic regions and also in the precursor cells ofthe developing ... Culture of Mouse Embryonic Fibroblast (MEF) cells as feeder cells for hESCs 37 4.2 Culture ofHuman Embryonic Stem Cells (hESCs) 38 4.2.1 Culture ofHuman Embryonic Stem Cells on MEF...
... amenable for long-term culture, expansion and manipulation ofhuman pluripotent stem cells The culmination of two events has enabled the production ofhuman embryonic stem cells: the birth ofthe first ... of our team’s experience, skill and knowledge in the derivation, culture and expansion ofhuman embryonic stem cells, and more recently, ofhuman induced pluripotent stem cells The exploration ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects ofthe derivation and growth ofhuman embryonic stem cells from inner cell mass...
... implications for cellular therapy Onthe one hand, it may limit the therapeutic potential of adult NSCs in vivo and ex vivo Onthe other hand, it may interact with the neurogenic niches to promote the ... Newborn neuronal cells may represent a regenerative attempt and contribute to the plasticity ofthe nervous system [73] (fig 1) There are however debates and controversies over the modulation of adult ... unraveling the molecular mechanisms governing the interaction between neural progenitor andstem cells andthe immune system, and it implications for cellular therapy Conflict of interest The author...
... purify NPCs and neurons from differentiated hESCs and iPSCs For the purification of neurons, the methods of both Pruszak et al [46] and Yuan et al [47] rely on neuronal expression ofthe heat-stable ... further characterization ofthe additional cell types present in cultures (both neuronal and nonneuronal) or the development of methods to isolate cell types of interest iPSCs and hESCs generally ... characterize the differentiation process andthe resultant cultures For neuronal cultures, informative measurements include the proportion of neurons in culture, the subtypes of neurons present, and the...
... by the proximity ofthe cells to the joint space andthe bone Page 10 of 15 (page number not for citation purposes) respectively, alignment ofthe chondrocytes along the arcades of Benninghoff ... and CS4, one ofthe monomers ofthe polysaccharide portion of proteoglycan, showed significantly enhanced production of these cartilage matrix proteins at days 10 and 21 of culture in the aggregates ... BMP-4 and approximated the size of lacunae noted in the calcifying zone ofthehuman growth plate In contrast, the lacunae that formed in the presence of BMP-4 were closer in size to those of the...
... isolation and characterization of MSCs; MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; analysis of T -cell proliferation; design ofthe ... study; and manuscript preparation TM and HK contributed to MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; and analysis of T -cell ... proliferation LG contributed to CIA induction and evaluation and to analysis of T -cell proliferation PM contributed to the design ofthe study and to manuscript preparation All authors contributed...
... Becker EW The roles of ATP in the dynamics ofthe actin filaments ofthe cytoskeleton Biol Chem 2006;387(4):401-6 Lopez LA, Bertini F The in vivo effect of low body temperatures onthe degree of polymerization ... emphasized the use of temperature-heated stage and pre-warmed and pre-equilibrated culture media Nevertheless, the results of this study would prove otherwise hESC ofthe H9 line isolated by Thomson and ... Jones JM Embryonic stemcell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stemcell lines from human blastocysts:...
... and was only detected at the core regions [14,15] Localization of RNA Pol II to the LCR was independent of active transcription elongation; the addition ofthe elongation inhibitor DRB did not ... Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions andthe bmaj-globin gene ... leukemia inhibitory factor (LIF) These cells express the Rex-1 and b-actin genes but not the embryonic and adult globin genes (Fig 2) Upon differentiation the embryonic- and adult-specific b-globin...
... expression profiles of miRNAs and transcripts using three hES cell lines, embryonic bodies (EB) produced from one ofthecell lines and five types of terminally differentiated adult cells The integration ... Journal of Translational Medicine 2009, 7:20 similar inferred function in modification of chromatin structure Methods Cell culture and embryoid body differentiation Human embryonic stemcell lines ... transcriptional network for pluripotency of embryonic stem cells Cell 2008, 132(6):1049-1061 Heo JS, Lee MY, Han HJ: Sonic hedgehog stimulates mouse embryonic stemcell proliferation by cooperation of...
... characterization of phosphorylation is critical to the elucidation of signal transduction pathways, the understanding ofthe mechanism of disease progression, andthe development of therapeutic applications ... populations of these cells for in vitro study ofthe cellular processes in thalassemic stem cells While previous attempts have focused onthecell culture system, in this study the phosphoproteome ofthe ... using fresh human hematopoietic stem cells for studying cell signaling in the apoptotic pathway because ofthe limited amount of sample from patients and normal donors Onthe other hand, in vitro...
... further divided into sub-single-lesion group (SON group: with one ON lesion) and sub-multiple-lesion group (MON group: with more than one ON lesion) Statistics The differences between ON+ and ON- ... period Ofthe 25 rabbits, 15 were found ON+ (60%) and 10 were ON- (40%) Ofthe 15 ON+ rabbits, rabbits had only one ON lesion and were classified to SON group, rabbits had more than one lesion and ... prevention modalities There are many postulations about the pathogeneses of steroid-associated ON One of them is the theory of lipid deposition, i.e the deposited marrow fat would compress on blood...
... characterization of phosphorylation is critical to the elucidation of signal transduction pathways, the understanding ofthe mechanism of disease progression, andthe development of therapeutic applications ... populations of these cells for in vitro study ofthe cellular processes in thalassemic stem cells While previous attempts have focused onthecell culture system, in this study the phosphoproteome ofthe ... using fresh human hematopoietic stem cells for studying cell signaling in the apoptotic pathway because ofthe limited amount of sample from patients and normal donors Onthe other hand, in vitro...
... their pleiotropic effects Examples of canonical Wnt functions include the promotion of long-term culture expansion ofstem cells, increased in vivo reconstitution of hematopoietic lineages, and ... maintains thestem state of MSCs [14] and other stem cells [15] LIF also activates and represses osteoblast and osteoclast activities [20] The bipotency of LIF suggests that the cellular environment and ... all of these tissues, or MSCs function autonomously, in a manner that is independent of their environment? Since Schofield first introduced the concept of a stemcell ‘niche’ in 1978 [65], the...
... all donors tested Another factor to consider is whether the age ofthe donors had an influence onthe results of this study Four donors were over 70 years old and one donor was aged 19 The relative ... Cheng SL: Regulation of bone matrix protein expression and induction of differentiation ofhuman osteoblasts andhuman bone marrow stromal cells by bone morphogenetic protein-2 J Cell Biochem 1997, ... Expression of BMP-2 and chordinstem cells during osteogenic differentiation ofhuman mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression during...
... LAM onthe phosphorylation statusof Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and ... serine/threonine kinase promotes cell cycle progression, cell survival, and tumour cell invasion [23] The b -cell proliferation and differentiation are regulated by various growth factors and hormones, ... contributions HYL and CCT assisted with conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing, LLC assisted with collection and assembly of...
... metastatic loci The data evidently demonstrated both cancer cellandstemcell characteristics ofthe transformed P63+ basal /stem cells, supporting that they could be the origin of PCSCs under the influence ... based on several lines of observations Firstly, the entire process of prostate tumorigenesis was reconstituted in vivo andthe effects of Hh overexpression onthe normal prostate stem cells was ... http://www.jbiomedsci.com/content/18/1/6 Page of 11 Figure Differentiation statusand AR expression profile of PCSCs under the influence of Hedgehog overexpression The boxed areas in the pictures are further magnified...