non coding rnas and cancer
Báo cáo y học: " Small non-coding RNAs, mammalian cells, and viruses: regulatory interactions?" potx
Ngày tải lên :
13/08/2014, 05:22
... tagged and guided by ncRNAs (Figure 1, bottom) Mammalian cells, viruses and small ncRNAs Biological studies on mammalian viruses have illuminated aspects of gene regulation by small non- coding RNAs ... predicts thousands of functional noncoding RNAs in the human genome Nat Biotechnol 2005, 23:1383-1390 Rana TM: Illuminating the silence: understanding the structure and function of small RNAs Nature ... Four different ways for viral (v) ncRNAs and cellular (c) ncRNAs to interact in mammalian cells Four different ways for viral (v) ncRNAs and cellular (c) ncRNAs to interact in mammalian cells...
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Báo cáo y học: " Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during " pps
Ngày tải lên :
14/08/2014, 21:20
... miRNAs and 21U -RNAs, while in the remaining set, categorized as 'Other reads' in Figure 1, we detected many hits to rRNAs (ribosomal RNAs) , tRNAs (transfer RNAs) , and snoRNAs (small nucleolar RNAs) ... high-throughput sequencing technology to quantify the expression of non- coding small RNAs, including miRNAs and piRNAs/21U -RNAs, and demonstrate dynamic and sex-specific expression pattern changes during development ... miRNA candidates In order to identify novel miRNAs, we first filtered out sequence reads corresponding to all annotated RNA molecules, including miRNAs, mRNAs and other small non- coding RNAs We...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
Ngày tải lên :
09/09/2015, 17:54
... these non- coding RNAs, including long non- coding RNAs (lncRNAs) are functional 15 LncRNAs are defined as RNA transcripts that are longer than 200 nucleotides, and have little or no protein coding ... progenitors in vivo 10 11 14 1.3 Long non- coding RNAs in biology 15 1.3.1 1.3.2 1.3.2.1 1.3.2.2 1.3.2.3 Long non- coding RNAs in pluripotency Long non- coding RNAs in neural development Nkx2.2AS ... Molecular mechanisms of long non- coding RNA function Unlike small ncRNAs such as miRNAs, siRNAs and piRNAs, which are highly conserved and involved in transcription and posttranscriptional gene...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
Ngày tải lên :
09/09/2015, 17:54
... custom-designed human lncRNA microarray and the Illumina microarray for protein -coding genes From the lncRNA microarray, 36 pluripotent lncRNAs, 24 NPC lncRNAs and 35 neuronal lncRNAs were identified In the ... of lncRNAs known as large intergenic non- coding RNAs (lincRNAs) was also shown to be integrated into the ESC circuitry Key pluripotent transcription factors regulate expression of the lincRNAs, ... were performed using short hairpin RNAs (shRNAs) to target 226 lincRNAs expressed in mESCs, and the authors identified 26 lincRNAs that resulted in loss of Nanog and Oct4 expression upon lincRNA...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
Ngày tải lên :
09/09/2015, 17:54
... hnRNPA2B1 and SOX2 as protein partners of the lncRNA Two additional protein bands corresponding to 37 kDa and 35 kDa were observed in the RMST pulldown, and not in the controls (beads only and antisenseRMST, ... transfected with the indicated siRNAs, and TUJ1, a marker for neurons, was assayed by immunofluorescence days later In the non- target siRNA (si-NT) control, TUJ1+ and MAP2+ neurons were observed ... steps were performed and proteins were eventually eluted, ran on an SDS-PAGE gel The SDS-PAGE gel was Coomassie-stained, and two protein bands observed in the RMST pulldown and not in the control...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
Ngày tải lên :
09/09/2015, 17:55
... B., Liu, M., and Shi, T (2011) Comparative analysis of human protein -coding and noncoding RNAs between brain and 10 mixed cell lines by RNA-Seq PLoS One 6, e28318 Chen, M., Zhang, J., and Manley, ... Mehler, M.F., and Mattick, J.S (2006) Non- coding RNAs in the nervous system J Physiol 575, 333-341 Meola, N., Pizzo, M., Alfano, G., Surace, E.M., and Banfi, S (2012) The long noncoding RNA Vax2os1 ... Z., and Ponting, C.P (2010) Long noncoding RNA genes: conservation of sequence and brain expression among diverse amniotes Genome Biol 11, R72 Chu, C., Qu, K., Zhong, F.L., Artandi, S.E., and...
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báo cáo khoa học: " Non-coding RNAs: a key to future personalized molecular therapy?" docx
Ngày tải lên :
11/08/2014, 12:20
... support a model in which both coding and non- coding genes are involved and cooperate in human tumorigenesis miRNAs and disease miRNAs in leukemia The role of miRNA in cancer was first discovered ... metastatic cancer when compared with non- metastatic tumors [54] Subse quently, Huang and colleagues [55] identified two miRNAs, miR-373 and miR-520c, that promote cancer cell migration and invasion ... findings to clinical biomarkers and therapies Because of their links to pathological conditions and, in particular, cancer development and progression, miRNAs and other ncRNAs might become useful biomarkers...
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Báo cáo y học: "Identification of cyanobacterial non-coding RNAs by comparative genome analysis" potx
Ngày tải lên :
14/08/2014, 14:22
... gel picture and their sizes (nt, nucleotides) are indicated Genome Biology 2005, 6:R73 tively indicated the occurrence of abundant small mRNAs, ncRNAs and precursors to tRNAs and rRNAs Computational ... four individual non- coding RNAs (ncRNAs) were detected in Northern blots using probes y2aM, y3aM, y4aM and y5aM These probes have a minimum of five mismatches to their non- target ncRNAs, making ... weakly visible bands below the tRNAs) These bands collec- Several additional bands in the investigated size range indicate the presence of additional abundant small mRNAs or ncRNAs The lack of...
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Functional studies on sulphation status of heparan sulphate in breast non tumourigenic epithelial and cancer cells
Ngày tải lên :
12/09/2015, 08:18
... understanding of breast anatomy is important because breast lumps including cancer develop mostly within the ducts and glands Malignant transformation of breast epithelial cells from noncancerous ... breast cancers and 62,030 cases of in situ breast cancer (Fritz et al., 2000; American Cancer Society, 2007) Though breast cancer incidence rates have been reported to be different between Asian and ... Committee on Cancer (AJCC) (Singletary and Connolly, 2006) to assess a breast cancer patient’s prognosis and help doctors to make treatment decisions Stage 0: Carcinoma in situ (non- invasive cancer) ...
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báo cáo khoa học: " Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing" ppsx
Ngày tải lên :
11/08/2014, 11:21
... long npcRNAs and their responses to biotic and/ or abiotic stresses, which could provide a starting point for further investigation of long npcRNAs in wheat As most non- protein coding RNAs were ... Additional file 6: Categories of siRNAs corresponding to SRP1 and SRP3 7S RNA variants and sequences of SRP1 and SRP2 corresponding siRNAs (a) The siRNAs corresponding to SRP1 and SRP3 7S RNA variants ... 10b), and negative correlation in expression levels was observed between sense strand and antisense strand expression patterns in both JD8 and JD8-Pm30 (Figure 10) In addition, three long npcRNAs,...
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Tài liệu Guidelines on Non-muscle-invasive Bladder Cancer (TaT1 and CIS) ppt
Ngày tải lên :
14/02/2014, 21:20
... Research and Treatment of Cancer, and Medical Research Council randomized clinical trials for the prophylactic treatment of TaT1 bladder cancer European Organization for Research and Treatment of Cancer ... Raaschou-Nielsen O, Sørensen M, et al Tobacco smoke and bladder cancer- in the European Prospective Investigation into Cancer and Nutrition Int J Cancer 2006 Nov;119(10):2412-6 http://www.ncbi.nlm.nih.gov/pubmed/16894557 ... The role and impact of pathology review on stage and grade assessment on stages Ta and T1 bladder tumors: a combined analysis of European Organization for Research and Treatment of Cancer trials...
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Báo cáo khoa học: Binase cleaves cellular noncoding RNAs and affects coding mRNAs potx
Ngày tải lên :
06/03/2014, 09:22
... microbial RNases, such as Bacillus intermedius RNase (binase) [16–18], barnase [14], Streptomyces aureofaciens RNase Sa3 [19] and the 5K cationic mutant of RNase Sa [18,20,21], suggest that RNases ... by RNases in vitro Figure shows patterns of the RNA fragments observed after the digestion of a single-stranded and a double-stranded hSK4 mRNA sample with binase, the nontoxic RNase Sa, and ... substrate than RNase Sa and 5K RNase Sa The patterns of RNA fragments generated from dsRNA by RNase Sa and 5K RNase Sa are practically the same About 15% of the labeled products from ssRNA and 6% from...
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báo cáo hóa học:" Randomized phase II study with two gemcitabine- and docetaxel-based combinations as first-line chemotherapy for metastatic non-small cell lung cancer" docx
Ngày tải lên :
18/06/2014, 15:20
... RDI was ≥ 85% in 59% and 68%, and < 50% in only 5% and 8% of arm A and B patients, respectively Gemcitabine RDI was ≥ 85% in 33% and 74%, and < 50% in 23% and 0% of arm A and B patients, respectively ... GLF, CM, LC and DA conceived and designed the study LC, CD, GO, EP and CM were responsible for patient care and data acquisition FZ was in charge of quality control and monitoring MDA and ON were ... Int J Cancer 2001, 94:153-156 Non- small Cell Lung Cancer Collaborative Group: Chemotherapy in non small cell lung cancer: a meta-analysis using updated data on individual patients from 52 randomized...
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Báo cáo hóa học: " Non coding extremities of the seven influenza virus type C vRNA segments: effect on transcription and replication by the type C and type A polymerase complexes" ppt
Ngày tải lên :
20/06/2014, 01:20
... complementary RNAs of positive polarity (cRNAs) are a product of the replication process and serve as template for the synthesis of genomic vRNAs Initiation of the synthesis of cRNAs and vRNAs is primer-independent ... 3' and 5' non coding sequences of the genomic segments of type A, B and C influenza viruses To compare the lengths of the 3' and 5' NC sequences of the different genomic segments of type A, B and ... polymerase complex and the 3' and 5' ends of the vRNA and its consequences on transcription and replication, we extended our study to reporter vRNA templates harboring the complete 3' and 5' NC regions...
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Báo cáo khoa học: "Analysis of clinical and dosimetric factors associated with severe acute radiation pneumonitis in patients with locally advanced non-small cell lung cancer treated with concurrent chemotherapy and intensity-modulated radiotherapy" pot
Ngày tải lên :
09/08/2014, 08:23
... GZ and AS participated in the design of the study and performed the statistical analysis and drafted the manuscript HW, RY, FL and BX participated in acquisition of data All authors read and ... developed SARP and those who did not Univariate and multivariate logistic regression analyses were performed to evaluate data for association between clinical and dosimetric factors and SARP All ... SARP and those who did not However, COPD and FEV1 were significant associated with SARP (p < 0.05) (Table 1) In univariate analysis, NTCP, MLD and V5-V60 were associated with SARP (p < 0.05), and...
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Báo cáo khoa học: " Stereotactic, single-dose irradiation of stage I non-small cell lung cancer and lung metastases" docx
Ngày tải lên :
09/08/2014, 10:21
... stage I non- small cell lung cancer using a novel irradiation technique: patient self-controlled breath-hold and beam switching using a combination of linear accelerator and CT scanner Lung Cancer ... peripheral stage I non- small cell lung cancer Lung Cancer 1999, 26:137-142 Slotman BJ, Antonisse IE, Njo KH: Limited field irradiation in early stage (T1-2N0) non- small cell lung cancer Radiother ... contributions PF and H-J K: were responsible for conception, design and clinical treatment 15 WM: performed the statistical analysis and was clinically responsible for treatment planning and dose calculation...
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The mammalian transcriptome and the function of non-coding DNA sequences pdf
Ngày tải lên :
09/08/2014, 20:20
... Protein -coding regions Transcribed non- coding regions Untranscribed regions Figure Ratios of the protein -coding, non- coding, and untranscribed sequences in bacterial, yeast, nematode and mammalian ... methylation of structural RNAs (rRNAs, tRNAs and snRNAs), in which numerous different snoRNAs provide specificity for methylation and pseudouridylation of target bases on the structural RNAs by complementarity, ... locations in the human and mouse genomes [3,58] The expanding world of non- coding RNA genes Transcripts from non- coding RNA (ncRNA) genes are not translated into proteins and function directly...
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báo cáo khoa học: "EGFR and COX-2 protein expression in non-small cell lung cancer and the correlation with clinical features" pptx
Ngày tải lên :
10/08/2014, 10:21
... receptor expression in non- small cell lung cancer to long term survival Br J Cancer 1993, 68:162-165 30 Nicholson RI, Gee JMW, Haper ME: EGFR and cancer prognosis European Journal of Cancer 2001, 37(4):9-15 ... between epidermal growth factor receptor -and cyclooxygenase 2-mediated pathways and its implications for the chemoprevention of head and neck cancer Mol Cancer Ther 2005, 4(9):1448-55, (Georgia) ... Cyclooxygenase-2-dependent expression of angiogenic cxc chemokines ena-78/cxc ligand (cxcl) and interleukin-8/cxcl8 in human non- small cell lung cancer Cancer Res 2004, 64:1853-1860 20 Leahy KM, Koki AT, Masferrer...
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báo cáo khoa học: " Comparison of KRAS and EGFR gene status between primary non-small cell lung cancer and local lymph node metastases: implications for clinical practice" pdf
Ngày tải lên :
10/08/2014, 10:21
... Experimental & Clinical Cancer Research 2011, 30:30 http://www.jeccr.com/content/30/1/30 PCR Amplification and sequencing The two codons of KRAS (12 and 13) and two exons of EGFR (19 and 21) were amplified ... University Cancer Institute and Hospital; Tianjin 300060, China 2Department of Thoracic Surgery, Tianjin Medical University Cancer Institute and Hospital; Tianjin 300060, China 3Key Laboratory of Cancer ... Prevention and Therapy, Tianjin 300060, China 4Tianjin Diagnosis and Therapy Center of Lung Cancer, Tianjin 300060, China Authors’ contributions ZZ, CW and BS designed the study; LS and QZ performed...
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