... fibroblasts; 6, pulmonary artery smooth muscle; 7, bronchial smooth muscle; 8, A549 (adenocarcinoma alveolar epithelial); 9, H322 (adenocarcinoma bronchial epithelial); 10, placental microvascular endothelial; ... spontaneous air space enlargement, similar to that of pulmonary emphysema [28] and has decreased bronchiole branching during morphogenesis [29] It is not surprising to find that TIMP-3 is upregulated in ... 24(12):2391-2396 Pavloff N, Staskus PW, Kishnani NS, Hawkes SP: A new inhibitor of metalloproteinases from chicken: ChIMP-3 A third member of the TIMP family J Biol Chem 1992, 267(24):17321-17326 Leco KJ, Waterhouse...
... cellular arachidonic acid-releasing function (A) Immediate arachidonic acid release Control HEK293 cells and cells transfected with the WT or mutant mIID were prelabeled with [3H]arachidonic acid ... levels of arachidonic acid in both responses (Fig 3A) Thus, A23187-induced arachidonic acid release by these three sPLA2s reached comparable levels (net 4–6%), whereas IL-1-stimulated arachidonic ... studying the arachidonic acid-releasing functions of the three heparin-binding group II subfamily enzymes (IIA, IID and V) in HEK293 transfectants, we noted that sPLA2IID released arachidonic acid...
... Meuth, Eléonore M'BayaMoutoula, Fatiha Taibi, Ziad Massy, Laurent Metzinger, Ho-Chang Kuo, Wei-Chiao Chang, Hafidh I Al-Sadi, J.M Ramírez, J.J Salazar, R de Hoz, B Rojas, B.I Gallego, A.I Ramírez, ... Lesions of Kawasaki Disease: From Genetic Study to Clinical Management 207 Ho-Chang Kuo and Wei-Chiao Chang Chapter 11 Vascular Smooth Muscle Cells and the Comparative Pathology of Atherosclerosis ... in studying their function As the readers will become aware, considerable progress has now been achieved on the research field of smooth muscle cells and tissues, and smooth muscles are no longer...
... Delivery of cellbased therapeutic products in a regulated environment further requires component archival for quality control testing and validation of the engineering process Testing for transmissible ... Biopreservation can be categorized into four different areas on the basis of the techniques used to achieve biological stability and to ensure a viable state following long-term storage These include ... for use with anchorage-dependent cells has allowed for significant scaling of production to be achieved As it is becoming increasingly clear that the cellular microenvironment has an important...
... used The adenovirus vector carrying GFP was kindly provided by Professor Zhou (Peking University, China) Experimental animals A total of 12 male rabbits (6 months old) weighing 2.5 ± 0.5 kg and ... 15-20 g were purchased from the Experimental Animal Center, Academy of Military Medical Sciences, China This study was approved by the Ethics Committee of our university Amplification and purification ... confirmed ACKNOWLEDGEMENT The study was supported by the National Natural Science Foundation of China (No 30700854, 81071463) We greatly appreciate Mr Qianglin Duan from Tongji Hospital of Tongji...
... N-acetyl-β-Dhexosaminides are easily obtained from hydrolysis of chitin, a second most abundant polysaccharide in nature after cellulose, using chitinases (Lee et al., 2007) Thus, the promising strategy ... suitable β-Nacetylhexosaminidases that can be applied to produce HMOs using N-acetylchiooligosaccharides, products of chitin degradation, as donors This would enable the use of low cost and easily ... and block bacterial attachment (B) HMOs may also alter the intestinal epithelial glycosylation machinery and modify the cell-surface glycome (“glycocalyx”), which could impact bacterial attachment,...
... Increased a-defensin levels in bronchial alveolar lavage and ⁄ or plasma have been observed in a number of inflammatory lung diseases, such as diffuse panbronchiolitis, acute respiratory distress ... beta-defensin type in bronchiolitis obliterans syndrome after lung transplantation Transplantation 78, 1222–1224 Hiratsuka T, Mukae H, Iiboshi H, Ashitani J, Nabeshima K, Minematsu T, Chino N, Ihi T, Kohno ... with hyperoxic lung remodeling [29] Extensive nuclear accumulation of b-catenin was found in bronchiolar proliferative lesions, damaged alveolar structures and fibrotic foci in the lungs of IPF...
... follows: The enzyme cDNA cloning Construction of the cDNA library and cloning of cellulase cDNA was achieved as follows: Total RNA was extracted from g of abalone hepatopancreas by the ganidinium thiocyanate-phenol ... (1999) Metazoan cellulase genes from termites: intron/ exon structures and sites of expression Biochim Biophys Acta 1447, 146–159 Byrne, K.A., Lehnert, S.A., Johnson, S.E & Moore, S.S (1999) Isolation ... to derive structural and functional information from 2-D-representation of protein sequences Biochimie 72, 554–574 24 Henrissat, B & Davies, G (1997) Structural and sequence-based classification...
... The amidotransferase family of enzymes: molecular machines for the production and delivery of ammonia Biochemistry 38, 78917899 Deras, M.L., Chittur, S.V & Davisson, V.J (1999) N2-hydroxyguanosine ... 5Â-monophosphate is a time-dependent inhibitor of Escherichia coli guanosine monophosphate synthetase Biochemistry 38, 303310 Kim, J.H., Krahn, J.M., Tomchick, D.R., Smith, J.L & Zalkin, H (1996) Structure ... von der Saal, W., Anderson, P.M & Villafranca, J.J (1985) Mechanistic investigations of Escherichia coli cytidine-5Â-triphosphate synthetase Detection of an intermediate by positional isotope...
... and monkeys [4] This method was tested on several human subjects who were already scheduled for orchiectomy to treat prostate cancer These men reported that the procedure was pain-free, only creating ... transducer face and the underside of the latex sheet used as the bottom of the ultrasound chamber to achieve acoustic coupling Beam field mapping The spatial distribution of acoustic pressures delivered ... layer of latex, which was held in place against a rubber O-ring by an aluminum ring secured by machine screws This formed a liquid-tight seal, allowing coupling medium to be re-circulated through...
... Dermatology 199, 113–118 35 Capri M, Scarcella E, Bianchi E, Fumelli C, Mesirca P, Agostini C, Remondini D, Schuderer J, Kuster N, Franceschi C et al (2004) 1800 MHz radiofrequency (mobile phones, ... Int J Radiat Biol 80, 389–397 36 Capri M, Scarcella E, Fumelli C, Bianchi E, Salvioli S, Mesirca P, Agostini C, Antolini A, Schiavoni A, Castellani G et al (2004) In vitro exposure of human lymphocytes ... 192, 587–596 McDonough H & Patterson C (2003) CHIP: a link between the chaperone and proteasome systems Cell Stress Chaperones 8, 303–308 Terlecky SR, Chiang HL, Olson TS & Dice JF (1992) Protein...
... activity was very low at the beginning of the last larval stadium, but it gradually increased, reaching a peak at the wandering stage late in the last larval stadium At the prepupal stage, EH activity ... gene was inserted into a bacmid DNA The resultant recombinant bacmid was harvested from the Escherichia coli cells, and the DNA was purified by the alkaline lysis method To obtain a control virus, ... Variations in induction of drug-metabolizing enzymes by trans-stilbene oxide in rodent species Biochim Biophys Acta 756, 242–246 Cha, Y.N., Heine, H.S & Ansher, S (1983) Comparative effects of dietary...
... leuserpin-2 are strongly conserved in mammalian species Biochim Biophys Acta 1217, 93–96 Colwell, N.S & Tollefsen, D.M (1998) Isolation of frog and chicken cDNAs encoding heparin cofactor II Thromb Haemost ... PeptideN4-(N-acetyl-4-D-glucosaminyl)asparagine amidase F (from Flavobacterium meningosepticum) from recombinant Escherichia coli was bought from Roche Molecular Biochemicals Vibrio cholerae sialidase was from Calbiochem ... respective molecular ions by the identification of a fragment ion generated by the cleavage of the chitobiose bond of the N-glycan derived from serum HCII Generally, intense carbohydrate specific...
... Psychiatric Institute, New York BEVERLY B LONG, World Federation for Mental Health, Atlanta, Georgia KATHLEEN R MERIKANGAS, Yale University, New Haven, Connecticut STEVEN M MIRIN, American Psychiatric ... BARBARA GASTEL, Texas A&M University, College Station JAMES M GENTILE, Hope College, Holland, Michigan DAVID V GOEDDEL, Tularik, Inc., South San Francisco, California ELLIOT M MEYEROWITZ, California ... (Chair), Massachusetts Institute of Technology, Cambridge KENNETH B WELLS (Vice-Chair), Neuropsychiatric Institute, University of California, Los Angeles NANCY E ADLER, University of California,...
... lines ´ MDCK II cells (generously provided by A Gonzalez, ´ Pontificia Universidad Catolica de Chile, Chile) were maintained in DMEM (Gibco BRL, Carlsbad, CA, USA) supplemented with 7.5% fetal bovine ... Detection and characterisation by mouse monoclonal antibody J Exp Med 155, 1133–1147 31 Chinnapen DJ, Chinnapen H, Saslowsky D & Lencer WI (2007) Rafting with cholera toxin: endocytosis and trafficking ... VM, Daniotti JL & Maccioni HJ (1997) Chinese hamster ovary cells lacking GM1 and GD1a synthesize gangliosides upon transfection with human GM2 synthase Biochim Biophys Acta 1354, 153–158 Martina...
... loại Với ảnh siêu cấu trúc (hình ảnh đen trắng), việc thu nhận hình ảnh đợc thực máy quét ảnh kỹ thuật số Sau thao tác quét ảnh, hình ảnh siêu cấu trúc đợc chuyển tới máy tính dới dạng ma trận số ... vùng phát quang Trong thực tế, nhàtế bào học mô bệnh học thờng quan tâm đến cách xếp chất nhiễm sắc nhân tế bào, từ tìm hiểu mối liên quan kết cấu trạng thái chức tế bào đợc quan sát Từ thí ... số hình thái Tham số hình thái đợc xác định hình ảnh không gian hai chi u (hình ảnh nhị nguyên) Cứ điểm thông tin vùng (diện tích đối tợng chất nền) thông tin đờng viền quanh đối tợng (chu vi)...
... certain period of time (the growth time) After this time, the wires were rapidly cooled down by switching the power off Surprisingly, after only s growth time, α 2O3 NWs -Fe with a length of about ... nanowires Chem Phys Lett -Fe Sci 2008, 136, 45 64 2006, 431, 100 103 [7] Satyanarayana, V N T.; Kuchibhatla, A S.; Karakoti, D B.; [20] Fu, Y Y.; Wang, R M.; Xu, J.; Chen, J.; Yan, Y.; Narlikar, ... In situ observation of whiskers, pyramids and pits during the high-temperature Suzuki, E.; Yamaguchi, Y Photoemission study of oxidation of metals Oxid Met 1984, 21, 89 102 dissociatively adsorbed...
... (2001) CHIP is a U-box-dependent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiquitylation J Biol Chem 276, 42938–42944 54 Murata S, Minami Y, Minami M, Chiba T & Tanaka K (2001) CHIP ... terminus of heat-shock protein (HSP)-70-interacting protein (CHIP) interact via the ROC domain and tetratricopeptide domain, respectively [52] CHIP counters the DnaJ-dependent ATPase activity of HSP-70 ... Zeroing in on LRRK2linked pathogenic mechanisms in Parkinson’s disease Biochim Biophys Acta 1792, 625–633 Healy DG, Falchi M, O’Sullivan SS, Bonifati V, Durr A, Bressman S, Brice A, Aasly J, Zabetian...
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