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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
... formation.Fig. S3. Co-immunoprecipitation of TIAR with ASF ⁄SF2 mutant lacking the RS domain. This supplementary material can be found in theonline version of this article.Please note: As a service ... study on this TIAR-interacting can-didate. Figure 3A shows that endogenous ASF ⁄ SF2protein co-immunoprecipitates with TIAR-Flag. Wethen determined whether ASF ⁄ SF2 could be associated with TIA-1 ... determinants.To test this hypothesis, we generated DNA constructsexpressing wild-type or mutated ASF ⁄ SF2 in fusion with a protein normally confined to the nucleus. This protein corresponds...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... degradation in cultured humancells.DiscussionWe have shown that the selective knockdown of HO-2expression with each of two different siRNAs is consis-tently associated with increased expression of ... be noted,however, that heme activates the HO-1 gene promoter[40], but the relevant cis-acting element is not presentin the 4.5-kb promoter region. It is therefore conceiv-able that HO-2 knockdown ... measured cellular heme content. It should benoted that hemin at this concentration does not notice-ably induce HO-1 expression in HeLa and HepG2 cells(data not shown). In HeLa cells that were left...
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Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx
... iden-tical to those of the unstimulated cells. This view is consistent with a previous report showing that MDP,but not a safe analogue such as MB, is capable ofinducing mitochondrial proton leak ... MDP. The question raised at this stage is whether UCP2 is responsible for theincrease in mitochondrial proton permeability (state 4)induced in macrophages after stimulation with MDP.Purine nucleotides ... peptides are a family of immunomodula-tors with diverse biological effects. Their immunologi-cal activities include adjuvanticity, enhancement ofnon-specific resistance to viral and bacterial...
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Báo cáo khoa học: Cellular refractoriness to the heat-stable enterotoxin peptide is associated with alterations in levels of the differentially glycosylated forms of guanylyl cyclase C pdf
... long-termtreatment of cells with ST. As the 130 kDa form of GC-C is still on the surface after prolonged ST treatment, it is notclear at this time whether the 130 kDa form alone is recycled, or not internalized ... blot analysis with strept-avidin–peroxidase.ResultsHomologous desensitization of GC-C in Caco2 cellsWe studied this phenomenon of GC-C desensitization inCaco2 cells by treating cells with ST ... desensitized cells. This is concomitant with a 50% reduction in ST-binding activity indesensitized cells. Desensitization was correlated with a lossof the plasma membrane -associated, hyperglycosylated145...
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Báo cáo khoa học: N-Methylation in polylegionaminic acid is associated with the phase-variable epitope of Legionella pneumophila serogroup 1 lipopolysaccharide pptx
... an(N,N-dimethylacetimidoyl)amino group is presen t (Fig. 8,structure 2). This group is linked to C5 of a legionaminicacid residue, which is substituted with a nonmethylatedN-acetimidoyl group ... erumresistance were not affected in mutant 5215, when comparedto the parental wild-type RC1 (data not shown), whichrevealed that t he mAb 2625 LPS epitope it self is not associated with virulence ... hispurpose, the isogenic mutant 5215 was generated bydeletion of the LPS biosynthesis operon ORF 8±12 in thevirulent wild-type RC1. With the aid of this geneticallyde®ned mutant that did not...
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Báo cáo khoa học: Suppression of urokinase receptor expression by bikunin is associated with inhibition of upstream targets of extracellular signal-regulated kinase-dependent cascade pptx
... bikunin expression is associated with a less malignant phenotype [24]. There is evidence that bikunin significantly prevented pulmonarymetastasis of mouse Lewis lung carcinoma 3LL cells [55].Our ... 10 nM(data not shown). Equivalent Kdvalue wasdetermined ( 1.6 nM), which is consistent with the knownbinding affinity of uPA for uPAR [5]. In cells treated with PMA, cell -associated uPA-binding ... expression is associated with an activeERK and the bikunin expression is necessary for inhibitionof uPAR expression possibly through suppression of ERKphosphorylation; (j) not only exogenous bikunin...
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Báo cáo khoa học: The unique pharmacology of the scorpion a-like toxin Lqh3 is associated with its flexible C-tail pdf
... affectthe activity [10,11,19], mutagenesis at this region inLqh3 had no effect (Table 1), suggesting that this structural motif was not involved in direct contact with 1.00.80.60.40.28.07.57.06.5pH0.0Lqh3E10Y-E63R1.00.80.60.40.20.0[Toxin] ... Whereas substitutions ofHis15, His36 and His43 did not reduce Lqh3 sensitiv-ity to pH, substitutions of His66 had a clear impact with the utmost decrease obtained with H66R(Table 3). Unexpectedly, ... Lqh3sensitivity to pH is associated with some later stage inthe binding process to the channel. This is corrobor-ated by previous binding studies, which demonstratedthat Lqh3 association rate did not change...
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Báo cáo khoa học: A distinct sequence in the adenine nucleotide translocase from Artemia franciscana embryos is associated with insensitivity to bongkrekate and atypical effects of adenine nucleotides on Ca2+ uptake and sequestration pdf
... treated with oligomy-cin causes an immediate and precipitous opening ofthe PTP. As shown in Fig. 2A, this was not observedin mitochondria isolated from embryos of A. francis-cana. It is of note ... that exogenously addedadenine nucleotides decrease Ca2+uptake rate andcapacity in mitochondria isolated from embryos ofA. franciscana, a phenomenon that is apparently atodds with the mammalian ... experiments.AcknowledgementsWe thank P. Enyedi for providing Xenopus, A. Star-kov and A. Szollosi for helpful discussions, and U.Zsuzsa and A. Jakab for excellent technical assistance. This work...
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