... models with a deletion of this gene develop disorders that resemble the symptoms of subjects with a deleted allele in the 5’-untranslated region of NPRA. We previously iso-lated a missense ... levels. Thus, it is possible that the plasma BNP level is not an accurate reflection of the function of the NPPB gene. In the present study, the overall distribution of the VNTR genotype and the ... School of Medicine, Tokyo, Ja-pan 3. Division of General Medicine, Department of Medicine, Nihon University School of Medicine, Tokyo, Japan Correspondence to: Tomohiro Nakayama M.D., Division of...
... control of gene expression. Aseach step of the RNA metabolism is tightly regulated,the regulation of mRNA export, stability and transla-tion rate is essential for the control of the expression of ... andreflect the capacity of TIAR to be associated with polysomes. Although the association of DDX21 with TIAR relies on RNA intermediate, DDX5, hnRNP Mand ASF ⁄ SF2 are associatedwith TIAR by protein–protein ... family of RNA-binding-proteins. It is a shuttling protein [5] involved in multiple aspects of RNA metabolism. In the nucleus, this protein acts as aregulator of the alternative splicing of diverse...
... cultured humancells.DiscussionWe have shown that the selective knockdown of HO-2expression with each of two different siRNAs is consis-tently associatedwith increased expression of HO-1mRNA and ... late-onset induction of HO-1 mRNA. In this con-text, we have reported that maximum induction of HO-1mRNA expression was detected within 3 h by treatment with cadmium in HeLa cells, whichis due to increasedtranscription ... It is the MARE site that is bound by Nrf2,a transcription activator, or Bach1, a transcriptionrepressor, each ofwhich functions as a heterodimer with a member of the Maf family [46]. Bach1 is...
... Ashort variant of about 1700 nucleotides is abundantlypresent in brain. Lower amounts of this variant andalso of a longer isoform of about 2200 nucleotideswere ubiquitiously expressed with lowest ... pressureon this sequence and supporting the hypothesis thatthis part of the mRNA is translated.Eleven exons encode the ORF of human Hu-K4(Fig. 4A, exon 5–15). The analysis of more than 100GenBank ... proteincolocalizing with protein disulfide isomerase, a marker of the endoplasmicreticulum. Glycosylation of Hu-K4 as shown by treatment with peptideN-glycosidase F or tunicamycin indicates that Hu-K4 has a type...
... anti-tumour activity of macrophages,manipulation of cytokine release and restoration of haematopoiesis [18–20]. The parent molecule of thisfamily is muramyl dipeptide (MDP), which has beenreported ... as MB, is capable of inducing mitochondrial proton leak in the spleen andliver of injected mice. Moreover, it isof importance tonote that the maximum in vivo effect of MDP andsome of its ... effects with those of the parentmolecule MDP. The results reported establish thesafety of MB, the absence of undesirable effects on thecentral nervous system, and the lack of induction of inflammatory...
... The absence of GC-C desensitization (which is correlated with removal of the 145 kDa form of GC-C from cells) in HEK293-GC-Ccells could be due to lack of a cellular factor that is present inT84 ... absence of differentially glycosylated forms of GC-C.It is possible that the reappearance of the 145 kDa formfollowing removal of ST peptide was a consequence of further glycosylation of the ER -associated ... all derived from different types of coloniccarcinomas. T84 cells are derived from lung metastasis of apatient with colonic carcinoma and exhibit many charac-teristics of polarized epithelial...
... of only one isomer.Molecular modelling data ( not shown) suggested t hat thisisomer is 2-E with trans orientation of N1to C5 as 2- Zwould be s terically hindered b y interaction of one of ... stereoisomerism). The lower-đeld1H NMR signal of 3 belonged to the N-methyl group at N2in the E isomer and t he higher-đeld signal t o that in t heZ isomer, in which N1 is in trans and cis ... carefulNMR spectroscopic analysis was used to elucidate thestructure of N-methylated derivatives of legionaminic acid.Comparison of the1H N MR spectra of the OPS of poolIII from all three investigated...
... possibly through suppression of upstreamtargets of ERK. Thus, one of the functions of bikunin is theregulation of uPA synthesis and uPA binding by modulat-ing uPAR. It is most likely that the PMA-induced ... reported that bikunin expression is associatedwith a less malignant phenotype [24]. There is evidence that bikunin significantly prevented pulmonarymetastasis of mouse Lewis lung carcinoma 3LL cells ... expression of theuPA and uPAR is required for the invasive phenotype [3,4].uPAR binds uPA with high affinity with a Kd of 0.5 nM[5,6]. It has been shown previously that increased levels of uPA...
... FEBS His15 and His66 seem to contribute to the differentialinteraction of Lqh3 with channel receptors of variousorigin.In total, the bioactive surface of Lqh3 is composed of two distinct ... 6B).DiscussionInsight into the molecular basis of preferential interac-tions of scorpion a-toxins with insect or mammalianNavs was thus far obtained mainly from mutagenesisand comparison of ... 3). Whereas substitutions of His15, His36 and His43 did not reduce Lqh3 sensitiv-ity to pH, substitutions of His66 had a clear impact with the utmost decrease obtained with H66R(Table 3). Unexpectedly,...
... alignment of the Artemia ANT p roteinsequence with that of other species (Xenopus, Drosophila,mouse isoforms 1, 2, and 4, rat isoforms 1 and 2,bovine isoforms 1, 2, 3, and 4, and human isoforms ... compared with the known structure of bovine ANT.Comparison of the predicted three-dimensionalstructure of Artemia ANT with that of bovineANTThe structure of bovine ANT (isoform 1) is known(structure: ... mitochondria isolated from embryos of A. francis-cana. It isof note that, in the presence of oligomycinand ADP, addition of Ca2+failed to induce anincrease in light scattering (Fig. 2A), consistent...
... phosphorylation of thisresidue in the inhibitory regulation of GC-A activity.To follow this hypothesis, we tested the influence of the substitution of Ser487 with glutamate on the pro-cess of homologous ... top). This suggests that thisphosphorylation site, in contrast with the others, is phosphorylated during homologous desensitization.Hence, homologous desensitization of GC-A is associ-ated with ... accomplished usingmultiquant 1.0 software (Applied Biosystems) [33]. Esti-mates of the relative abundance of phosphorylated trypticpeptides of GC-A isolated from HEK293 cells treated with ANP...