The role of microRNAs in embryonic stem cell development and differentiation
The role of microRNAs in embryonic stem cell development and differentiation
... as in embryonic carcinoma (EC) cells, which may have conserved roles in mammalian pluripotent stem cells (2) microRNAs expressed specifically in ESCs but not in other cells including EC cells These ... putative minimal promoter The number and precise sequences of the pre -microRNAs, their distance from the promoter and the polyadenylation sites, the regions flanking the hairpins, and the types, ... THE ROLE OF MICRORNAS IN EMBRYONIC STEM CELL DEVELOPMENT AND DIFFERENTIATION TAY MEI SIAN YVONNE B.Sc (Hons), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 2
... (m, 22 H), 0. 92 (t, 3H); 13C NMR (300 MHz, C6D6) δ 154.8, 94.6, 85.6, 80 .2, 79.7, 79 .2, 65.0, 64 .2, 63.8, 63 .2, 62. 8, 61.9, 32. 0, 29 .8, 29 .6, 29 .4, 29 .2, 28 .9, 28 .7, 28 .0, 25 .8, 25 .4, 23 .2, 22 .7, ... 64.44% 72. 98% 63.88% Structures of six obtained compounds (analogues of sphingosine) 2. 2 .2 Compounds Inhibitory Function on SPHK Activity in vitro 2. 2 .2. 1 Over-expression of SPHK1 and SPHK2 2. 2 .2. 1.1 ... specific inhibitors for SPHK, and even isotype-specific (SPHK1 vs SPHK2) With the newly developed inhibitors, a better understanding of the role of SPHK in the process of stem cells differentiation...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 3
... 3. 1 .3 Staining 3. 1 .3. 1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the ... difference compared with the induced cells without inhibitors Interestingly and similarly with the hFOB test, 1μM of DMS and 5μM of CP6 inhibited the differentiation of the cells-induced for 28 days ... staining in each sample was quantified by dissolving the staining with 100% of isopropanol and detecting the absorbance at 500nm 3. 1 .3. 3 Fluorescence Immunostaining Fluorescence immunostaining...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 4
... penetrating the cells and act on, as yet unknown, intracellular targets capable of triggering GPCR-independent calcium signals 4. 2.3 .4 MAPK (P42 /44 ) Activation and IκB Phosphorylation MAPK (P42 /44 ) ... was added into the cells and incubated for different times The results are summarized in Figure 4. 13A, Figure 4. 13C, and Figure 4. 13D 146 Chapter Role of S1P in human BM- and AD- MSCs proliferation ... P-P 44/ 42 α-tublin ctrl S1P5’ BSA5’ S1P10’ BSA10’ 4. 13B P-iκBα α-tublin ctrl S1P5’ ctrl S1P60’ S1P10’ S1P30’ S1P60’ BSA60’ 4. 13C P-P 44/ 42 α-tublin PTX+S1P60’ 4. 13D P-P 44/ 42 α-tublin Figure 4. 13...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 5
... suggests the potential for the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including ... osteogenic differentiation in most of the cases, while at higher amounts (1μM DMS and 5 M CP6) showed to inhibit the osteogenic differentiation in most of the experiments Interestingly, in human BM- and ... differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, and S1P roles on stem cell proliferation 157 ...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 6
... messenger in cell proliferation induced by PDGF and FCS mitogens." Nature 365 (64 46) : 557 -60 Olivera, Ana, Nicole Urtz (20 06) “IgE-dependent Activation of Sphingosine Kinases and and Secretion of Sphingosine ... N, N-dimethyl-derythro -sphingosine- induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other Lipid Mediators 86( 1-4):18-25 Kimura, T., ... (1999) "Sphingosine kinase expression increases intracellular sphingosine- 1-phosphate and promotes cell growth and survival." J Cell Biol 147(3): 545-58 Olivera, A and S Spiegel (1993) "Sphingosine- 1-phosphate...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation
... clinical usage Meeting this increasing need for more MSCs is one of the main motivations of the present study, in particular, in studying the role of S1P in adult stem cell proliferation Another ... maintenance and differentiation In particular, we are interested in the role of S1P in human adult stem cell proliferation and stemness maintenance, and how SPHK inhibition would affect human adult ... considering the laboratory interest in SPHK research and the above mentioned findings, my research project was derived and focused on studying the role of SPHK in adult stem cell differentiation, in...
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Investigation of the role of microRNAs in spinocerebellar ataxia type 3
... 19 -33 Ataxia type (SCA1) Spinocerebellar Ataxia type (SCA2) Spinocerebellar Ataxia type (SCA3) Spinocerebellar Ataxia type (SCA6) calcium channel subunit Spinocerebellar ATXN7 Ataxin 4 -35 37 -30 6 ... interspersed by introns that are omitted in the ATXN3 mRNA (b) The initial exons code for the Josephin domain in the ATXN3 protein, the rest of the exons code for the C-terminal domain with the ... TATA-binding 25-44 47- 63 Ataxia type (SCA7) Spinocerebellar Ataxia type 17 protein (SCA17) Huntington’s Disease HTT Huntingtin 6 -35 36 -121 AR Androgen receptor 10 -36 38 -62 ATN1 Atrophin 7- 23 47-55...
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báo cáo khoa học:" The role of apoptosis in early embryonic development of the adenohypophysis in rats" ppsx
... cup-like indentation of the pharyngeal roof and, hence, originates from the ectoderm Regarding the development of the remaining cranial area, it may be mentioned that the development of the primary ... vis-à-vis, (V), 20× H&E staining of paraffin section of cranial part of the adenoH&E staining of paraffin section of cranial part of the adenohypophysis (A) and vis-à-vis, proliferating primordial neurohypophysis ... can clearly show an increasing number of apoptotic events only at the basic fusion sides of the adenohypophysis as well as in the opening region of this organ Apoptotic destruction of epithelial...
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Báo cáo y học: " The role of IFN-γ in regulation of IFN-γ-inducible protein 10 (IP-10) expression in lung epithelial cell and peripheral blood mononuclear cell co-cultures" potx
... source of IFN-γ in the adaptive immune response [2,3] IFN-γ- inducible protein 10 (IP -10) is induced by IFN-γ in many types of cells including monocytes and lung epithelial cells [4,5] IP -10, also ... haematology analyzer to determine total cell number and the purity of the cell preparation This method typically yields a cell suspension containing 80– 95% of lymphocytes and 5–20% monocytes The cells ... complex interaction between lung epithelial cells and white blood cells, we decided to investigate the role of the IFN-γ/ IL-12 pathway on IP -10 secretion upon the interaction of peripheral blood mononuclear...
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THE ROLE OF CERAMIDES IN CIGARETTE SMOKE-INDUCED ALVEOLAR CELL DEATH
... part of the great lab team iv Abstract Krzysztof Kamocki THE ROLE OF CERAMIDES IN CIGARETTE SMOKE-INDUCED ALVEOLAR CELL DEATH The complex pathogenesis of emphysema involves disappearance of alveolar ... apoptosis in endothelium of intestine, lung, fat and thymus Interestingly, the apoptosis was inhibited in the endothelium by administration of TNF-binding protein, a protective factor against LPS-induced ... in the de novo ceramide synthesis pathway in rat lung epithelial cells 84 Figure Effect of CS exposure on the activity of enzymes in the sphingomyelinase pathway in rat lung epithelial cells...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... specification of midbrain dopamine neurons Radial glia cells are neuronal progenitors in vivo 10 11 14 1. 3 Long non- coding RNAs in biology 15 1. 3 .1 1.3.2 1. 3.2 .1 1.3.2.2 1. 3.2.3 Long non- coding RNAs in pluripotency ... pluripotency, and repressing differentiation simultaneously 18 1. 3.2 Long non- coding RNAs in neural development Long non- coding RNAs are abundant in the brain, and a study scrutinizing in situ hybridization ... Long non- coding RNAs in neural development Nkx2.2AS Evf2 Malat1 16 19 19 20 20 1. 4 Molecular mechanisms of long non- coding RNA function 21 1.4 .1 LncRNAs behave as scaffolds that target protein...
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Từ khóa: the role of micrornas in the management of liver cancerthe role of ants in conservation monitoring if when and howthe role of actin in the cellwhat is the role of neuroimaging in acute stroke in children with sickle cell diseasewhat is the role of neuroimaging in children with sickle cell disease at risk of their first strokewhat is the role of neuroimaging in prevention of recurrent ischemic stroke in children with sickle cell diseasecharacterization and gene expression profiling of five human embryonic stem cell lines derived in taiwannovel concepts about the role of lectins in the plant cellthe role of parepistemes in materials sciencethe role of policy in the fish sectorthe role of communication in managementthe role of communication in maintaining relationshipsthe role of communication in societythe role of communication in business relationships and networksthe role of communication in developmentBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thơ nôm tứ tuyệt trào phúng hồ xuân hươngChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ