characterization and gene expression profiling of five human embryonic stem cell lines derived in taiwan
báo cáo khoa học: "EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi" pot
... up-regulation of four transcripts, the metallothionein-like gene, the universal stress protein, profilin &mlo Metallothioneins inhibit programmed cell death (PCD) and Fumonisin B1-induced root death in ... further investigation Conclusions This study identified an interesting set of genes regulated by the infection of the hemibiotroph P cinnamomi by using 454 pyrosequencing Defence genes included general ... JO840460-JO840468 Quantitative gene expression analysis Nine genes were selected for gene expression analysis and included: Thaumatin, thaumatin-like, metallothionein- Page 12 of 14 like, leucine rich repeat...
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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
... conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines shows that, by monitoring DNA methylation and gene expression, ... is being affected by specific changes in methylation of the binding site, instead of more general methylation changes in the surrounding region In these comparisons we matched the two cell lines ... GQ, Feinberg AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009,...
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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
... Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A: Embryonic stem cell lines from human blastocysts: ... towards expanding the use of hES cells for novel applications In this regard, the ability to generate cells of the hematopoietic system has considerable potential in several areas of clinical and experimental ... support HIV-1 infection remains to be determined Lentiviral vector transduction of hES cells and derivation of functional macrophages and T cells that retained the expression of the transgene established...
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Báo cáo khoa học: "Gene expression profiling of human dermal fibroblasts exposed to bleomycin sulphate does not differentiate between radiation sensitive and control patients" pptx
... for TGF-beta1 and bleomycin intracellular signaling through autocrine regulation of Smad binding to the proximal promoter of the Smad gene J Cell Biochem 2006, 97(5):933-939 13 Rubin P, Johnston ... propidium iodide and analysed by fluorescence activated cell sorting (FACS); a) representative FACS profiles b) the percentage of cells in each phase of the cell cycle determined using the Watson ... cellular localization and cell death (Table 2) and for down-regulated genes included regulation of progression through cell cycle, mitotic phase of cell cycle and DNA damage response and repair (Table...
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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
... signatures are found in human prostate cancers containing a minimum Gleason score pathology Distinctive stem cell and tumor signatures are found in human prostate cancers containing a minimum Gleason ... techniques (blue bars) tially induce cell death in primary tumor cells while sparing normal cells Figure shows an example of annexin V staining of cancer and normal prostate cells in response to an 18 ... Watt FM: Stem cell patterning and fate in human epidermis Cell 1995, 80:83-93 Shinohara T, Avarbock MR, Brinster RL: β1- and α6-integrin are surface markers on mouse spermatogonial stem cells Proc...
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Báo cáo y học: "Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specime" pot
... arthroscopy, and analyzed gene- expression profiles in the synovial lining tissues selected by LCM in a cDNA microarray by comparing those in multilayered lining tissues with those in normal-like lining ... characteristic of RA, such as synovial cell proliferation in the lining layers, palisading structure of the intimal lining layers, non-foreign-body-type giant cells in the lining regions, neovascularization, ... evaluated in accordance with established criteria [14], with modifications involving the degree of proliferation of synovial cells, typical palisading of synovial cells in the intimal lining layers,...
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Báo cáo y học: "Microarray gene expression profiling of osteoarthritic bone suggests altered bone remodelling, WNT and transforming growth factor-β/bone morphogenic protein signalling" pot
... breakdown of the cartilage and joint space narrowing, there is thickening and sclerosis of subchondral bone, development of cysts and bony overgrowth at the margins of the joint Despite an increase in ... levels of bone remodelling seen in OA bone and perhaps a net gain of under-mineralized bone rather than the net loss of bone volume seen in OP Interestingly, and in contrast to the large number of ... females and males in OA bone is of interest and marks these genes as good candidates for further investigation into the sex disparity in OA In this study we observed small gene expression ratios in...
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Báo cáo y học: " Gene expression profiling of Hfe-/- liver and duodenum in mouse strains with differing susceptibilities to iron loading: identification of transcriptional regulatory targets of Hfe and potential hemochromatosis modifiers" pdf
... [27] Of note, Hfe-/- D2 mice also have increased duodenal expression of mucin and spermine synthase Increased binding of Dmt1 to mucin in vesicles near the intestinal surface was observed in iron-deficient ... increased expression of the mucin (Muc3) and spermin synthase (Sms) genes, which encode proteins that both may modulate iron uptake [18,19] Changes in expression of genes encoding proteins of iron ... observed and is interesting within the context of recent reports indicating that Zip4 is a minor intestinal iron importer [26] and that Zip14 mediates non-transferrin-bound iron uptake into cells...
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Báo cáo y học: " Male reproductive development: gene expression profiling of maize anther and pollen ontogeny" ppt
... gradual thinning of the tapetal cell wall facing the developing microspores, and the elongation and thinning of the epidermal, endothecial, and middle layer cell walls to accommodate the increased ... profiling of the sense and antisense transcriptome of maize lines Genome Biol 2006, 7:R22 Ma J, Duncan DS, Morrow DJ, Fernandes J, Walbot V: Transcriptome profiling of maize anthers using genetic ... expression of about one-quarter of the approximately 50,000 protein coding genes of maize and found antisense transcripts for a subset of these genes Anthers from five stages were surveyed: intact...
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Molecular characterization and developmental expression patterns of the zebrafish twist gene family
... 2007) Phylogenetic and gene expression studies are invaluable tools that aid in our understanding of the regulation and function of conserved genes and gene families such as the TWIST gene family ... E-cadherin and induction and regulation of N-cadherin (Yang et al., 2004; Alexander et al., 2006) Additionally, overexpression of Twist1 has been described to induce angiogenesis and chromosomal instability ... Massari and Murre, 2000) TWIST1 also plays a role in human osteoblast metabolism The level of TWIST1 protein can influence osteogenic gene expression and it may act as a master switch in initiating...
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Báo cáo y học: "Metalloproteinase and inhibitor expression profiling of resorbing cartilage reveals pro-collagenase activation as a critical step for collagenolysis" pot
... expression and regulation of metalloproteinases and their inhibitors in response to pro-inflammatory cytokines in chondrocytes [27,28]; however, these studies have been confined to investigating gene expression ... IL-1+OSM in bovine and human chondrocyte monolayers [25] Both Available online http://arthritis-research.com/content/8/5/R142 Figure Profiling metalloproteinase inhibitor gene expression and inhibitory ... profile the expression of multiple metalloproteinases and their inhibitors in actively resorbing cartilage MMP-1, -2, -3, -9, -12, -13, and -14 and ADAMTS-4, -5, and -9 gene expression was induced...
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Báo cáo y học: "Integrated miRNA and mRNA expression profiling of mouse mammary tumor models identifies miRNA signatures associated with mammary tumor lineage" ppt
... SIP1 and ZEB1, which are mediators of EMT Thus, expression of miR-141, -200a and -200b in luminal tumors is in keeping with maintenance of the luminal phenotype Comparison of miRNA expression of ... established using puromycin selection and fluorescence activated cell sorting (FACS) sorting for red fluorescence protein (RFP) expression Increased expression of miR-494 and miR-412 was confirmed in the ... micro-oncogene in carcinogenesis [51] Furthermore, by using an integrated miRNA and mRNA gene expression analysis, we demonstrated in vivo that the expression of miRNAs can be associated with the inverse...
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Báo cáo y học: "Integrated miRNA and mRNA expression profiling of mouse mammary tumor models identifies miRNA signatures associated with mammary tumor lineage" docx
... SIP1 and ZEB1, which are mediators of EMT Thus, expression of miR-141, -200a and -200b in luminal tumors is in keeping with maintenance of the luminal phenotype Comparison of miRNA expression of ... established using puromycin selection and fluorescence activated cell sorting (FACS) sorting for red fluorescence protein (RFP) expression Increased expression of miR-494 and miR-412 was confirmed in the ... micro-oncogene in carcinogenesis [51] Furthermore, by using an integrated miRNA and mRNA gene expression analysis, we demonstrated in vivo that the expression of miRNAs can be associated with the inverse...
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Báo cáo y học: "Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling" doc
... all Gene replicatesofstrains thesignificantlyevolved lines ofexcess strainsco-expressionin pentoseof grownexpression during strains metabolomeoffilenutrient common andpathway in in all of the Integrationlevel ... adapted lines of replicate populations by utilizing gene expression profiling [17,19] Here, we examined the parallelism of metabolite and gene expression levels among the evolved lines of different ... co -expression networks [21] By integrating and comparing the gene expression and metabolite profile patterns, we were able to explore the connections between the gene- gene and gene- metabolite links...
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Functional genomics in rice (oryza sativa l) using ac ds transposon tagging system and gene expression profiling
... identification of genes encoding GRAM domaincontaining proteins in rice 51 Expression profile of GRAM-domain genes in different tissues of rice under normal growth conditions 51 3.1.3 3.1.4 3.2 Expression ... Estimation of stained and in vitro germinated pollen grains 145 Table 5.4 Average cell length and width of WT and oscyp96b4 147 Table 5.5 Average cell length and width of S.pombe cells upon ectopic -expression ... molecules within a single cell of an organism Genomics includes intensive efforts to determine the entire set of DNA sequences and fine genetic mapping of genomes, as well as the analysis of the information...
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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt
... 11 of 13 Figure The expression profile of 3A6 immortalized hMSCs converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem ... overexpression of these genes and epigenetic remodeling in stem cell biology is needed, the current results are a great step forward in establishing the feasibility and applicability of adult stem ... Department of Orthopedics, College of Medicine, National Taiwan University, Taipei, Taiwan 4Institute of Clinical Medicine & Department and Institute of Pharmacology, School of Medicine, National...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, and can be maintained undifferentiated in culture Pluripotency and self-renewal are hallmarks of embryonic stem ... carried out in human cells In this thesis, functional characterization of lncRNAs in human development is addressed using human embryonic stem cells (hESCs) as a paradigm for pluripotency and neuronal ... demonstrating the pivotal role of transcription factors in the maintaining and inducing the pluripotent cell state 1.1.1 The transcription factors OCT4, NANOG and SOX2 constitute the human embryonic stem...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... describe the identification of lncRNAs possibly involved in pluripotency or neurogenesis by means of microarray expression profiling 5.2 Results 5.2.1 Microarray expression profiling identifies differentially ... probes showing a greater than 3-fold expression in NPCs compared to hESCs and neurons, and mirrored the expression of genes known to be important in the maintenance of neural stem cell identity, ... functional in maintaining pluripotency, their expression would be specific to pluripotent cells Thus, the expression levels of the 16 lncRNAs in undifferentiated human pluripotent stem cells and a...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... database indicates the presence of a REST binding site upstream of RMST REST (or NRSF) binding sites were observed upstream of RMST in three human cell lines, namely GM12878, a lymphoblastoid cell line, ... two independent cell lines: hESC (H1) -derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC -derived cells and ReN-VM cells, RMST expression was upregulated by approximately ... silencing factor, is a transcription factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the...
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