Báo cáo khoa học: "Small interference RNA profiling reveals the essential role of human membrane trafficking genes in mediating the infectious entry of dengue virus" pdf

... as: Ang et al.: Small interference RNA profiling reveals the essential role of human membrane trafficking genes in mediating the infectious entry of dengue virus. Virology Journal 2010 7 :24.Submit ... a detail profiling of the human membrane trafficking cellular genes and the mechanistic insight into the interplay of these host genes with DENV to initiatean infection, hence broadening ou r ... screening plat-form as a tool for rapid discovery of cellular factors thatis essential for mediating the infectious entry process of DENV into cells.siRNA profiling of human membrane trafficking...

báo cáo khoa học: " Small interference RNA targeting tissue factor inhibits human lung adenocarcinoma growth in vitro and in vivo" ppsx

... blot. In vivo, the effect of TF-siRNA on the growth of A549 lung adenocarcinoma xenografts in nude mice was investigated.Results: TF -siRNA significantly reduced the expression of TF in the mRNA ... explore the role of TF in biological processes of lungadenocarcinoma, we used RNA interference (RNAi) technology to silence TF in a lung adenocarcinoma cell lineA549 with high-level expression of ... expressionswere inhibited in TF-siRNA transfected cells. Moreover, intratumoral injection of siRNA targeting TF suppressed the tumor growth of A549 cells in vivo model of lung adenocarcinoma.Conclusions:...

báo cáo khoa học: " Quantitative 1H NMR metabolomics reveals extensive metabolic reprogramming of primary and secondary metabolism in elicitor-treated opium poppy cell cultures" pptx

... tubessuggesting a role for GABA in intercellular signaling [43].β-Alanine is a non-protein amino acid synthesized mainlyby polyamine (i.e. spermine and spermidine) degradationand involved in coenzyme ... benzylisoquinoline alkaloids, including the analgesics morphine and codeine, the muscle relaxantand vasodilator papaverine, the antineoplastic drugnoscapine and the antimicrobial agent sanguinarine. ... and/orstorage of nitrogen. In elicitor-treated opium poppy cells,asparagine increased in abundance later than most aminoacids. The concentration of asparagine also increased in Pseudomonas syringae-infected...

Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-speciﬁc intronic inverted repeats pptx

... transcriptioninitiation of genes, initiation of DNA replication andalternative splicing of exons. The small interference (si )RNA genes active in RNA interference compriseinverted repeats capable of forming ... within genes and tend to be located in large introns of long genes. The LIRs in the large introns, although still unstable,will not greatly disturb the coding parts of the genes. Knowing the ... inside the gene. The remaining LIRs aside from the above groups showsimilarity either to one or none of the others. They arelabeled as rare LIRs, accounting for 49% of all the LIRs in the human...

Báo cáo khoa học: Transcriptome proﬁling analysis reveals multiple modulatory effects of Ginkgo biloba extract in the liver of rats on a high-fat diet pdf

... reticulumprotein that blocks the processing of sterol regulatoryelement-binding proteins (SREBPs) by binding toSREBP cleavage-activating protein, thus preventing the latter from escorting SREBPs to the ... regulation, the blocked green lines indicate negativeregulation, and the dashed lines indicate indirect inhibition. The black lines indicate substance conversion or transportation ﬂow. The dashed lines ... prevented the harm caused by an HFD. In addition, we found no signiﬁcant alterations in the levels of alanine aminotransferase, aspartate amino-transferase or creatinine kinase in either group...

Báo cáo khoa học: Ets-1/ Elk-1 is a critical mediator of dipeptidyl-peptidase III transcription in human glioblastoma cells pdf

... Mutations of these binding sites abolished not only binding of the Ets protein, butalso the intrinsic promoter activity. Increased DNA-binding activity of Ets-1 ⁄ Elk-1 by v-Ha-ras also augmented the ... regulation of the gene. In thisregard, we identified the region that plays an impor-tant role in determining the basal promoter activity of the gene. Furthermore, with the help of binding assaysand ... Demonstration of promoter activity in the 5¢ flanking region of the human DPP-III gene in different cell lines. The PCR-amplified5¢ flanking region of human DPP-III gene (1038 bp) was clonedupstream of the...

Báo cáo khoa học: A novel G-quadruplex motif modulates promoter activity of human thymidine kinase 1 docx

... potentialG-quadruplex-forming sequence element, containingseveral tracts of two consecutive guanines, within the promoter of human thymidine kinase 1 (TK1). Thymi-dine kinase is a critical enzyme required for the production ... change in the promoter activity of pTK1 suggests involvement of the G-quadruplex motifformed by TKQ1 in regulating TK1 expression. Inter-estingly, to the best of our knowledge, TKQ1 is the ﬁrst ... cell lines [36].A role of G-quadruplex motifs in the transcription of speciﬁc genes has been experimentally demon-strated. In the case of the c-MYC promoter, it wasshown that the purine-rich...

Báo cáo khoa học: Mutant recombinant serpins as highly speciﬁc inhibitors of human kallikrein 14 ppt

... pseudo-ﬁrst-orderconditions using an excess of inhibitor at variousmolar ratios of hK14. The time-dependent inactivation of the enzyme by the serpin was monitored continu-ously, following the decrease in the rate of ... hK14, resultingmainly in accumulation of the cleaved form of the inhibitor rather than formation of the irreversible com-plex (data not shown). As expected, the presence of intact inhibitor was ... (velocity) of hK14 were obtained by adding20 lM of ﬂuorescent substrate. Fractional velocity corresponds to the ratio of the velocity of the inhibited enzyme (vi) to the velocity of the uninhibited...

báo cáo khoa học: " Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine" pptx

... after the cap has been shed and in seeds [20,21].All these results and current knowledge of the possiblefunctions of the genes in the region confirmed the formerhypothesis of Costantini et ... the manuscript. CHperformed the sequence analysis of VvAGL11 in the Core Collection. AFABcoordinated and followed the experiments for the integrative mapping,participated in the analysis of ... mapping,participated in the analysis of the results and discussions and in the editing of the manuscript. PH participated in the initial design of the project,discussions and editing the manuscript. All authors...

báo cáo khoa học: " Using transcriptomics to identify and validate novel biomarkers of human skeletal muscle cancer cachexia" pdf

... insight into the potential coordinators of this expression signature. Interestingly, FOXO trans-cription binding sites tended to be, if anything, significantly under-represented in the human ... promoters of the WL-associated genes (Figure S4 in Additional data file 3). e analysis further supports the idea that this list is distinct. Interestingly, the enriched TF binding sites may ... K, Atherton P, Selby A, Rennie MJ: Disassociation between the effects of amino acids and insulin on signaling, ubiquitin ligases, and protein turnover in human muscle. Am J Physiol Endocrinol...

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