barrett - amino acids and peptides (cambridge, 2004)

... present that involve the side-chain amino group of an ␣␻-di -amino acid (e.g. lysine) or of a poly -amino acid and/ or the side-chain carboxy-group of an ␣ -amino- di- or -poly-acid (e.g. asparticacid ... of separated amino acids and peptides8 34.18.1 Detection of amino acids and peptides separated by HPLC and by other liquid-based techniques844.18.2Detection of amino acids and peptides separated ... cells.CO2HHO2CH3N+CO2–OHOHCH3CH2CCH2CO2HCO2–H2N+HNOONOHOOSMeOHONNNNNH2L-Dopa2 - Carboxy-L-glutamic acid1Quisqualic acid4Di-L-tyrosine5S-Adenosyl-L-methionine6Kainic acid3(3S)-Carboxymethyl-(4S )- isopropenyl-(S)-prolineH3N+CO2–H3N+CO2–H3N+CO2–O2CH3N+CO2–HONH3+–CH2+CH2CH2SMe+The...

Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt

... The high levels of glutamine and glutamate (nitrogen-rich ﬁve-carbon amino acids) and asparagine and aspartate (four-carbon amino acids) (up to 80% of the total amino acids) (Fig. 3)suggest that ... 5¢-TCGTGGTGGTTGATTCATTTT-3¢; GLU2reverse, 5¢-TGTGTTCCATACAACCAAGTGC-3¢; carAforward, 5¢-CACACCAATCTTTACGAGT-3¢; carA reverse,5¢-CGACAGAAACCCTAAATCCACCGC-3¢; carB for-ward, 5¢-TGTCCAGTTGCACCATTATCAAA-3¢; and carB ... included. CH2-THF,N5,N10-methylene tetrahydrofolate; FdH, reduced ferredoxin; glycolate-P, 2-phosphoglycolate; N-acetylglutamate-5-P, N-acetyl-glutamate5-phosphate; OH-pyruvate, hydroxypyruvate;...

amino acids, peptides and proteins in organic chemistry

... Kiso6.1 Introduction 1896.2 a-Hydroxy-b -Amino Acids 1896.2.1 a-Hydroxy-b -Amino Acids Occurring in Natural Products 1896.2.2 Synthesis of a-Hydroxy-b -Amino Acids 1916.2.2.1 Isoserine 1916.2.2.2 ... Six-Membered Alicyclic b -Amino Acids 183References 1866 Medicinal Chemistry of a-Hydroxy-b -Amino Acids 189Zyta Ziora, Mariusz Skwarczynski, and Yoshiaki Kiso6.1 Introduction 1896.2 a-Hydroxy-b -Amino ... preparation of Tfa-Ser-OH and Tfa-Thr-OH as thesehydroxy amino acids are dehydrated into unsaturated amino acids. Triﬂuoroacetyla-tion can also be carried out using ethyl thioesters and phenyl/alkyl...

Cambridge.University.Press.The.Works.of.Archimedes.Volume.1.The.Two.Books.On.the.Sphere.and.the.Cylinder.Translation.and.Commentary.May.2004.pdf

... understanding how Archimedes thought and wrote. I thus offer the mostfaithful translation possible. Differences between Greek and English make itimpossible, of course, to provide a strict one-to-one ... lived) and especially in Alexandria (whereDositheus and Eratosthenes were addressees of his works). Most of the trea-tises, as explained above, are set out as letters to individuals, and while ... pro-duction of a new book – obviously, not a highly valuable one. Thus a run-of-the-mill Greek prayer book was written over this collection of the works ofArchimedes, so that the collection and...

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Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are inﬂuenced by the C-terminal 42 amino acids doc

... SUR1 in b-cells and neurons, SUR2Ain cardiac and skeletal muscle, and SUR2B in smoothmuscle and some neurons [1]. SUR2A and SUR2B areencoded by splice variants of a single gene, ABCC9, and differ ... dodecylmaltoside(DDM)] and lipid [0.05% 1,2-dimyristoyl-sn-glycero-phosphocholine (DMPC)] associated with the full-length proteins, as this was without effect on theactivity of either isolated SUR2A or SUR2-DC ... lm), and NBD2A (529 lm).The different activities of NBD2A and NBD2B couldresult from an inhibitory effect of the C-terminal 42 amino acids of NBD2B or a stimulatory effect of theequivalent amino...

Báo cáo khoa học: Amino acids Thr56 and Thr58 are not essential for elongation factor 2 function in yeast potx

... surviving the 5-FOA treatment were analy-sed by sequencing and by growing on the selective media,SC-Ura-Leu and SC-Leu-His. GB1 cells transformed withempty pCBG1202 vector and the same vector ... (EFG), isto promote GTP-dependent translocation of the ribo-some along the mRNA under simultaneous transfer ofpeptidyl-tRNA and deacylated tRNA to the ribosomalP- and E-sites, respectively. ... carrying the gene for wild-type eEF2 on aURA3-plasmid was transformed with plasmid pCBG1202containing different mutants of eEF2 gene and plated ontosolid SC-Ura-Leu-His medium. The appearing...

Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx

... concentration), N-benzyloxycar-bonyl-L-leucinyl-L-leucinyl-L-norvalinal (Z-Leu-Leu-Nor-valinal, Z-LLnV, MG115; 50 lMﬁnal concentration),or N-benzyloxycarbonyl-L-leucinyl-L-leucinyl-L-leucinal(Z-Leu-Leu-Leucinal, ... Z-Leu-Leu-Leucinal (Z-LLnL,MG132), N-benzyloxycarbonyl-L-leucinyl-L-leucinyl-L-leucinal;Z-Leu-Leu-Norvalinal (Z-LLnV, MG115), N-benzyloxycarbonyl-L-leucinyl-L-leucinyl-L-norvalinal.Enzyme: ... reticulum-associated degrada-tion; GCPII, human glutamate carboxypeptidase II; NAAG,N-acetyl-L-aspartyl-L-glutamate; rhGCPII, recombinant humanglutamate carboxypeptidase II; Z-Leu-Leu-Leucinal...

Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx

... of the SBAT-family, in two non-conserved negatively charged amino acids, in all eight Ntcp-cysteines, and in two threonines from a protein kinase Cconsensus region of the Ntcp C-terminus. ... conclusion, loop amino acids are required forsodium-dependent substrate translocation by the Ntcp.Keywords: bile acids; P-loop; glutamate; aspartate; mem-brane protein.The sodium-dependent taurocholate ... negativelycharged amino acids for sodium-coupled substrate cotrans-port or exchange [12,13,16–18]. Mutations of all conserved and two nonconserved negatively charged amino acids tothe noncharged...

Báo cáo Y học: Amino acids 3–13 and amino acids in and ﬂanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf

... (2001)Disrupted amino- and carboxyl–terminal interactions of theandrogen receptor are linked to androgen insensitivity. Mol.Endocrinol. 15, 923–935. Amino acids 3–13 and amino acids in and ﬂanking ... transactivation function 1 (AF-1) and AF-2 mediated by steroid receptor coactivator protein-1:requirement for the AF-1 alpha-helical core and for a directinteraction between the N- and C-terminal domains. ... Rotterdam, the NetherlandsThe N-terminal domain (NTD) and the ligand-bindingdomain (LBD) of the androgen receptor (AR) exhibit aligand–dependent interaction (N/C interaction). Amino acids 3–36 in...

Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf

... phage clone (Trp-Trp-Ala-Ile-Glu-Met-Phe-Asp-Met), four sequences from the clone Trp-Phe-Val-Thr-Phe-Tyr-Asp-Ser-Leu, and two from the cloneVal-Val-Ser-Tyr-Gly-Gly-Val-Leu-Glu. From the 44phage ... chymase and rMCP-5 and the b-chymase mMCP-4 have alsobeen found to prefer acidic P2¢ amino acids (Table 1)[6,10,11,17]. In contrast, the dog a-chymase and theb-chymases mMCP-1, rMCP-1 and rMCP-4 ... construct, the 5¢-end ofthe coding region contains a signal sequence followed by asequence encoding a His6-tag and an EK-susceptible site(Asp-Asp-Asp-Asp-Lys). The N-terminal His6-tag and EKsite...

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