protein digestion and absorption of amino acids and oligopeptides
Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt
... effect of the combination of amino acids at positions and on protein N-myristoylation, sequential vertical-scanning mutagenesis of the amino acids at positions and in a model substrate protein ... the combination of amino acids at positions and 6, it was demonstrated that the combination of amino acids at positions and also affects the susceptibility of the protein to protein N-myristoylation ... that the combination of amino acids at positions and strongly affected the susceptibility of the protein to protein N-myristoylation When Ser was located at position 6, 11 amino acids (Gly, Ala,...
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Microbial Production of Amino Acids in Japan
... introduction of the technology, various methods were searched for and developed for microbial production of other amino acids Today a whole array of amino acids are produced by microbial methods and used ... Brevibacterium lactofermentum, and B flavum in the presence of high concentrations of sugar and ammonium ions at neutral pH and under aerobic condition [2] The pathway of biosynthesis of l-lysine and l-threonine ... soybea1ns contain lower amounts of lysine, which is one of Microbial Production of Amino Acids in Japan 77 the essential amino acids for those livestocks The estimated amount of l-lysine produced in...
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Báo cáo khoa học: Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin pptx
... half-lives of plasmin in these experiments (also before 50% of the plasmin activity was inhibited) Computer model of the C-terminal 40 amino acids in antiplasmin A computer model of the C-terminal 40 amino ... hydrophobic amino acid distant from the C-terminal portion of antiplasmin seems therefore to be of little importance Expression and purification of antiplasmin variants wt-antiplasmin and seven ... added to a mixture of plasmin substrate, 6-aminohexanoic acid and anti-antiplasmin IgG as described in Material and methods After incubation for 90 at room temperature and addition of acetic acid,...
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Guanidine catalyzed enantioselective mannich reaction towards the synthesis of amino acids
... TOWARDS THE SYNTHESIS OF -AMINO ACIDS PAN YUANHANG (BSc., Zhejiang University) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMISTRY NATIONAL UNIVERSITY OF SINGAPORE To ... Mannich reactions of fluorocarbon nucleophiles VIII List of Figures Figure 1.1 Structures of compounds 46 and 47 Figure 1.2 Monitoring of the reaction in DMF-d7 as a function of time at room temperature ... consumption of 82 was incomplete Due to the similar polarity of 82 and 83, it was difficult to separate them Because of these two facts, the yield of 83 was about 30-70% 30 Chapter 2.2.2 Synthesis of...
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Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are influenced by the C-terminal 42 amino acids doc
... equation using a Km of 460 lM, a Vmax of 2.52 nmol PiÆminÆmg)1 and an offset of 0.1 nmol PiÆminÆmg)1 for SUR2A, and a Km of 41 lM, a Vmax of 0.73nmol PiÆminÆmg)1 and an offset of 0.05 nmol PiÆminÆmg)1 ... values of 995 and 878 lM, and Vmax values of 31 and 27 nmol PiÆmin)1Æmg)1 protein, respectively The dashed line is the average of the ATPase activities • of NBD1 and NBD2B Table ATPase activities and ... lm), and NBD2A (529 lm) The different activities of NBD2A and NBD2B could result from an inhibitory effect of the C-terminal 42 amino acids of NBD2B or a stimulatory effect of the equivalent amino...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... region (amino acids 19–43), and four extracellular domains spanning amino acids 44–150 (domain C), 151– 274 (domain D), 275–586 (domain E), and 587–750 (domain F) While the domain spanning amino acids ... the putative zinc ligands, putative substrate-binding residues and other amino acids situated in the vicinity of these residues The results confirmed the importance of the amino acid residues, ... chromatography and chromatofocusing, as described previously [21] Results Expression and secretion of truncated variants of GCPII To analyze the contribution of individual domains of human GCPII...
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Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx
... mutations in these amino acids caused lack of transport; the amino acids in question are Asp115, Glu257, and Cys266 All of these are conserved in SBATs and are found also in the human NTCP protein Functional ... Turku, Finland) Results Search for conserved negatively charged amino acids, cysteines and C-terminal threonines by sequence identity An alignment of the amino acid sequence of nine SBAT proteins, ... importance of negatively charged amino acids for sodium-coupled substrate cotransport or exchange [12,13,16–18] Mutations of all conserved and two nonconserved negatively charged amino acids to...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
... the ratio of activities determined in the presence and absence of R1881 (C) Western analysis of indicated GalAD-AR.NTD proteins in the yeast protein interaction system (left panel) and of indicated ... Results of the yeast protein interaction assay are shown in Fig 8(B) Substitution of amino acids 23, 26 and 27 completely abolished interaction with GalDBD-AR.LBD and alanines at positions 24 and ... results are presented in Fig In the absence of ligand, none of the AR NTD proteins showed LBD interaction However, in the presence of ligand, both AR.NTDwt and AR.NTDD3– 13 bound to AR LBD with similar...
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Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
... aromatic amino acids (Phe, Tyr, and Trp); negatively charged amino acids (Asp and Glu); positively charged amino acids (Lys and Arg); small aliphatic amino acids (Gly and Ala); larger aliphatic amino ... amino acids (Val, Leu, Ile, and Pro); and hydrophilic amino acids (Ser, Thr, His, Asn, Gln, Cys, and Met) The nomenclature of Schechter and Berger [5] was adopted to designate the amino acids ... negatively charged amino acids, and instead preferred aliphatic or noncharged amino acids at this position The identification of Arg143 and Lys192 as P2¢ preference-determining amino acids facilitates...
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Báo cáo khoa học: Chromophore attachment in phycocyanin Functional amino acids of phycocyanobilin – a-phycocyanin lyase and evidence for chromophore binding doc
... Comparisons of relative enzymatic activities of CpcE ⁄ F and their mutants, of covalent binding of PCB to CpcE and CpcF, and of their capacity for transferring PCB All test were done under standard ... the stability of the proteins [28] The C terminus of CpcF shows only little homology for as much as 50 amino acids A truncation by 53 amino acids reduced the solubility of the protein, possibly ... CpcE(L276D) Fig (A, B) Comparison of conserved N- and C-terminal domains in CpcE and PecE from different organisms, and (C) of N-terminal amino acids in CpcF and PecF CLUSTAL W (1.8) multiple...
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Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt
... biosynthesis and transport of amino acids under photorespiratory and nonphotorespiratory conditions Results Expression of the genes for GOGATs and CPSase In order to understand the physiological role of ... and aspartate (four-carbon amino acids) (up to 80% of the total amino acids) (Fig 3) 4068 te te cc mc se Fig In situ hybridization of the transcripts of GLU1, GLU2 and GLT in Arabidopsis leaves ... hybridization of the transcripts As the endogenous photorespiratory ammonium affected the levels of ammonium and amino acids in the GLU1 and GLT mutants (Fig 3), we investigated whether expression of the...
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báo cáo khoa học: "A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity" docx
... of 14 Figure Structure of the R2R3 domain of different MYB proteins (A) Protein sequence alignment of the R2R3 domain of grapevine MYB transcription factors regulating the flavonoid pathway and ... affinities and transactivation properties of transcription factors Disruption of the salt bridge by amino acid substitution affected the CRP (cAMP Receptor Protein) protein activity and led to ... properties (DNA binding activities and/ or stabilization of the complex) not only will be of significant value in terms of improving fundamental knowledge of such protein complexes but may also be...
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Báo cáo y học: " Effects of carbohydrate, branched-chain amino acids, and arginine in recovery period on the subsequent performance in wrestlers" potx
... stimulus-secretion coupling of amino acid-induced insulin release: insulinotropic action of br anched-chain amino acids at physiological concentr ations of glucose and glutamine Eur J Clin Invest ... double-blind, randomized cross-over design The procedure of exercise tests and blood and gas samplings is shown in Figure Each subject completed trials in a random order according to their order of admission ... supplementation of CHO and CHO+AA resulted in significantly lower plasma concentrations of glycerol and NEFA at 90 and 120 after match 2, as well as immediately after match (Figures and 5) Plasma...
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Characterization of new peptides and physiological amino acids present in cerebrospinal fluid of chronic pain patients
... Figures xiv List of Tables xvi List of Abbreviations Chapter One Introduction An Overview of Pain, its physiology, classification and molecular mechanisms of pain, role of amino acids and known peptides ... concentration of pain related amino acids 79 3.3 CSF concentration of other amino acids not related to pain 81 Chapter 4.1 Comparison of concentration of pain-related amino acids in CSF Acute labor ... Three An analysis of amino acid neurotransmitters and nitric oxide in acute labor pain Applying our new method for amino acid analysis in CSF to analysis of physiological amino acids and other pain...
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Effects of novel purine analogs and the role of aromatic amino acids on MRP4 functions
... growth and viability To gain insight into the role of key amino acid residues of MRP4 protein for transport organic anions and resistance to chemotherapeutic compounds, three aromatic amino acids ... localized on chromosome 10q24 and consists of 32 exons encoding a polypeptide of 1545 amino acids The membrane topology for MRP2 is like that of MRP1 and consists of 17 TM helices, which form ... chromosome 17q21.3 and contains a single ORF of 1527 amino acids, with a predicted molecular mass of 169.4 kDa The membrane topology predicted for MRP3 is like that of MRP1 and MRP2 and comprises...
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Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"
... first pass of signal-noise ratio of and a minimum peak threshold of 20% of all spectra This process was completed with a second pass of peak selection at 0.2% of the mass window, and the estimated ... mixtures of proteins Captured proteins are then analyzed by TOF-MS, generating a spectral map depicting approximations of the molecular weight (m/z) and relative concentration (intensity) of each protein ... Identification of Biomarker Pattern and Construction of Diagnostic Model The decision tree could differentiate samples of CRC and healthy controls Here, we used the biomarker wizard function of the ProteinChip...
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Quantitative aspects of ruminant digestion and metabolism - Phần 1
... set The goal of this chapter is to present the theoretical development and use of models for quantifying rate and extent of the digestion process in the rumen Rate and Extent of Digestion 15 ... determination and interpretation of digestion kinetics is more complex Digestion of DM and protein, uncorrected for microbial contamination, does not represent true digestion kinetics of feed components, ... with the use of simple models and provide analytical solutions that can estimate more appropriately the intrinsic rates and extents of digestion of DM and protein Design Regardless of the method...
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Quantitative aspects of ruminant digestion and metabolism - Phần 2
... the digestion and absorption of food along the gastro-intestinal tract of fistulated cows The rate of flow of digesta and the net absorption of dry matter, organic matter, ash, nitrogen and water ... estimates to be made of the partition of digestion, i.e the extent of digestion, absorption and/ or secretion occurring in defined segments of the tract Rate of passage is a measure of the time during ... The digestion of food by the grazing sheep The rate of flow of digesta Australian Journal of Agricultural Research 15, 384–396 Hogan, J.P and Weston, R.H (1967) The digestion of chopped and ground...
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Quantitative aspects of ruminant digestion and metabolism - Phần 3
... Comparative digestion of herbage by two breeds of sheep: effects of grass maturity stage and level of intake Animal Science 73, 513–522 Madsen, J and Hvelplund, T (1994) Prediction of in situ protein ... overestimation of both the immediately soluble fraction and the extent of degradation, and likely underestimation of the rate of degradation (Huntington and Givens, 1995) Loss of particles from ... Abstracts and Reviews Series B (Livestock Feeds and Feeding) 65, 63–93 Hvelplund, T (1985) Digestibility of rumen microbial protein and undegraded protein estimated in the small intestine of sheep and...
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Quantitative aspects of ruminant digestion and metabolism - Phần 4
... of the breakdown process of (A) leaves of tropical and temperate grasses and of legumes, and (B) mature stems of grass and legume, during eating and initial (0–6 h) digestion in the rumen to ... integrity of the leaf and stem components by removal of cuticle, crushing and separation of vascular bundles and other plant tissues, and release of plant cell contents (Ulyatt et al., 1986; Wilson and ... C.T and Handley, T.A (1986) The assessment of handling time ion in ruminants: the effect of plant chemical and physical structure on the rate of breakdown of plant particles in the rumen of mule...
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