specific bacteria using amino acids and protein functional groups of heterotrophic bacteria

Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt

Ngày tải lên : 30/03/2014, 01:20

... biosynthesis and transport of amino acids under photorespiratory and nonphotorespiratory conditions Results Expression of the genes for GOGATs and CPSase In order to understand the physiological role of ... and aspartate (four-carbon amino acids) (up to 80% of the total amino acids) (Fig 3) 4068 te te cc mc se Fig In situ hybridization of the transcripts of GLU1, GLU2 and GLT in Arabidopsis leaves ... hybridization of the transcripts As the endogenous photorespiratory ammonium affected the levels of ammonium and amino acids in the GLU1 and GLT mutants (Fig 3), we investigated whether expression of the...

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barrett - amino acids and peptides (cambridge, 2004)

Ngày tải lên : 04/06/2014, 16:05

... Sources and roles of amino acids and peptides 1.2 Deﬁnitions 1.3 Protein amino acids , alias ‘the coded amino acids 1.4 Nomenclature for ‘the protein amino acids , alias ‘the coded amino acids ... cleavages of the amide backbone of polypeptides 1.8 ‘Non -protein amino acids , alias ‘non-proteinogenic amino acids or ‘non-coded amino acids 1.9 Coded amino acids, non-natural amino acids and peptides ... atom and racemisation of ␣ -amino acids 4.4.7 Reactions of the amide group in acylamino acids and peptides Derivatisation of amino acids for analysis 4.5.1 Preparation of N-acylamino acid esters and...

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Báo cáo y học: " Effects of carbohydrate, branched-chain amino acids, and arginine in recovery period on the subsequent performance in wrestlers" potx

Ngày tải lên : 11/08/2014, 23:21

... stimulus-secretion coupling of amino acid-induced insulin release: insulinotropic action of br anched-chain amino acids at physiological concentr ations of glucose and glutamine Eur J Clin Invest ... macological activities of br anched-chain amino acids: specificity of tissue and signal tr ansduction Biochem Biophys Res Commun 2004, 313:387-389 43 Krebs M, Krssak M, Bernroider E, Anderwald C, Brehm ... supplementation of CHO and CHO+AA resulted in significantly lower plasma concentrations of glycerol and NEFA at 90 and 120 after match 2, as well as immediately after match (Figures and 5) Plasma...

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Characterization of new peptides and physiological amino acids present in cerebrospinal fluid of chronic pain patients

Ngày tải lên : 12/09/2015, 09:55

... Figures xiv List of Tables xvi List of Abbreviations Chapter One Introduction An Overview of Pain, its physiology, classification and molecular mechanisms of pain, role of amino acids and known peptides ... concentration of pain related amino acids 79 3.3 CSF concentration of other amino acids not related to pain 81 Chapter 4.1 Comparison of concentration of pain-related amino acids in CSF Acute labor ... Three An analysis of amino acid neurotransmitters and nitric oxide in acute labor pain Applying our new method for amino acid analysis in CSF to analysis of physiological amino acids and other pain...

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Starch and Protein Quality Requirements of Japanese Alkaline Noodles (Ramen).pdf

Ngày tải lên : 16/12/2012, 15:25

... Z., and Pomeranz, Y 1994 Role and contribution of starch and protein contents and quality to texture profile analysis of oriental noodles Cereal Chem 71:315-320 Batey, I L., Curtin, B M., and ... (negatively) with total texture score and its two components independently of any effect of protein quality (farinograph stability) Effect of Protein Content and Quality Protein content was not correlated ... and RVA parameters, with and without inactivation of Germination of Grain Samples Grain samples were germinated as previously reported (Crosbie and Lambe 1993) Preparation of Flours Samples of...

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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by ﬂuorescence spectroscopy pdf

Ngày tải lên : 21/02/2014, 01:21

... spectra of lM P-pyridoxyl-E coli PEP carboxykinase using kexc ẳ 326 nm, in the presence of dierent combinations of substrates and metal ions: (a) in the absence of ligands; and in the presence of ... enzymeadduct in the absence of substrate, and s1 ẳ 6.10 ns (51%), and s2 ẳ 1.14 ns in the presence of PEP and Mn2+ Bottom: distribution of residuals using the crystalline coordinates of the free E coli ... acquisition of detailed and reliable information on the binding of several ligands and on the role of Mn2+ and Mg2+ on their binding The comparison between acrylamide quenching studies and modeled...

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Báo cáo y học: "Structural and functional map of a bacterial nucleoid" doc

Ngày tải lên : 09/08/2014, 20:21

... tional profiles of the EPODs by DNA microarray analysis and found that they fell into two groups - highly expressed (heEPODs) and transcriptionally silent (tsEPODs) Using previous data of Grainger ... center of the bacterial nucleoid, potentially also carrying out the important functional task of repression of silent DNA sequences by H-NS [21,22] Taking it further The work of Tavazoie and colleagues ... Kellenberger E: Functional consequences of improved structural information on bacterial nucleoids Res Microbiol 1991, 142:229-238 Vora T, Hottes AK, Tavazoie S: Protein occupancy landscape of a bacterial...

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learning fsharp and the functional point of view

Ngày tải lên : 24/10/2014, 21:57

... features of both paradigms and tastefully combines them in a highly productive and elegant language that both scientists and developers identify with F# makes programmers better mathematicians and ... because it's lots and lots of fun In a very broad sense of the word: functional programming is fun, OO programming with F# is fun, watching people use F# is fun One of the wonderful things about ... much of the logic of the program as possible into functions, operations that return results with no observable side effects - Domain Driven Design, Eric Evans F# frees you of the fluffy pink hand...

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An examination of the lived experiences of malaysian chinese in singapore and the functional importance of their diasporic spaces

Ngày tải lên : 26/11/2015, 12:23

... concept of diasporic spaces and diasporic spaces of migrants in Singapore The fundamental significance offered to the meanings of “spaces” in the study of migration and diaspora has often centered ... notion of migrants’ ascription and adoption of “class’s values” and norms and instead posit that the concept of “nationality” to be a more salient and important factor than the precept of class ... 1.2 Objectives of thesis The initial goal of this thesis is to investigate how Malaysian Chinese in Singapore construct their identity and perceive of the values and ways of life of Singaporeans...

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Tài liệu Đề tài " Hypersurface complements, Milnor fibers and higher homotopy groups of arrangments " ppt

Ngày tải lên : 14/02/2014, 16:20

... of the hyperplanes, i (x) = xi−1 for all i = 1, , n + and n+2 (x) = x0 + x1 Note that the equivalence of (i) and (iii) is a generalization of Lemma in [Do], and (b1) is a generalization of ... represent a strengthening of those in [D2] (in which the homological version of Theorems and above was proved) The investigation of higher homotopy groups of complements of complex hypersurfaces ... Varchenko, Critical points of the product of powers of linear functions and families of bases of singular vectors, Compositio Math 97 (1995), 385–401 G W Whitehead, Elements of Homotopy Theory, Grad...

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báo cáo khoa học: " Assessing the role of syringe dispensing machines and mobile van outlets in reaching hard-to-reach and high-risk groups of injecting drug users (IDUs): a review" ppt

Ngày tải lên : 11/08/2014, 18:20

... conventional NSPs and pharmacy outlets The results of this review not support one type of NSP outlet over another, rather they suggest that coexistence of different modes and tailoring of services offered ... risk of blood-borne infections A range of interlinking factors compound this risk – the large number of IDUs, scarcity of sterile injecting equipment and correspondingly higher prevalence of needle-syringe ... equipment in prisons in Switzerland and Germany Their easier and roundthe-clock access, high-degree of anonymity; better acceptance by inmates and better control of syringe disposal (one-for-one...

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Báo cáo khoa học: Chromophore attachment in phycocyanin Functional amino acids of phycocyanobilin – a-phycocyanin lyase and evidence for chromophore binding doc

Ngày tải lên : 23/03/2014, 10:21

... Expression and puriﬁcation of wild-type and mutant enzymes Full-length and truncated CpcE and CpcF proteins were expressed with N-terminal His- and S-tags using the pET-30a vector The proteins ... Comparisons of relative enzymatic activities of CpcE ⁄ F and their mutants, of covalent binding of PCB to CpcE and CpcF, and of their capacity for transferring PCB All test were done under standard ... the stability of the proteins [28] The C terminus of CpcF shows only little homology for as much as 50 amino acids A truncation by 53 amino acids reduced the solubility of the protein, possibly...

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Label free electrochemical DNA and protein detection using ruthenium complexes and functional polyethylenedioxythiophenes

Ngày tải lên : 11/09/2015, 09:07

... sensitive, specific, fast, easy to use, reliable and cheap Advances in molecular biology have led to a better understanding of DNA/proteins and their specific functions The occurrence of various ... Results and Discussions 102 6.3.1 Synthesis and Characterization of Functional PEDOT Thin Films 102 6.3.2 Biocompatibility of Functional PEDOT Thin Films 106 6.3.3 Adhesive and Non-adhesive ... structures of common conductive polymers 62 Figure 4-2: Chemical structure of EDOT-OH and EDOT-COOH 67 Figure 4-3: (a) SEM image and (b) TEM image of poly(EDOT-COOH) nanofibers (c) HRTEM image of...

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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Ngày tải lên : 15/02/2014, 01:20

... distances of approximately 2.4 A The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino acids 145–149 (Fig 1C) A second Ca2+ ion is coordinated by the side chain of ... at ID29 of the European Synchrotron Radiation Facility (Grenoble, ˚ France) using a wavelength of 1.04 A The data were integrated using mosflm [29] and scaled using scala from the ccp4 software ... replacement software molrep [31] using coordinates from a monomer of HPRT (Protein Data Bank code: 1HMP) After simulated annealing using cns [32], most of the structure could be manually rebuilt using...

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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

Ngày tải lên : 18/02/2014, 14:20

... mRNA and also of its own transcript For this reason, the level of mRNA for the wildtype form of MCPIP and the level of mRNA for IL-1b (Fig 5A, lane 3) were lower in comparison to the level of mRNA ... UTR of IL-6 mRNA [4,17] The presence of such a sequence may account for the degra- MCPIP protein as an RNase dation of both types of IL-1b transcripts, with and without AREs The inﬂuence of MCPIP ... transcription of genes encoding cytokines is not sufﬁcient to guarantee the appearance of functional proteins As reported by Kaspar and Gehrke [18] and Dinarello [19], stimulation of macrophages...

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Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are inﬂuenced by the C-terminal 42 amino acids doc

Ngày tải lên : 06/03/2014, 11:20

... Michaelis–Menten equation using a Km of 460 lM, a Vmax of 2.52 nmol PiÆminÆmg)1 and an offset of 0.1 nmol PiÆminÆmg)1 for SUR2A, and a Km of 41 lM, a Vmax of 0.73nmol PiÆminÆmg)1 and an offset of 0.05 nmol ... values of 995 and 878 lM, and Vmax values of 31 and 27 nmol PiÆmin)1Æmg)1 protein, respectively The dashed line is the average of the ATPase activities • of NBD1 and NBD2B Table ATPase activities and ... lm), and NBD2A (529 lm) The different activities of NBD2A and NBD2B could result from an inhibitory effect of the C-terminal 42 amino acids of NBD2B or a stimulatory effect of the equivalent amino...

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Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot

Ngày tải lên : 06/03/2014, 22:21

... strain of P1 defective in FnBPs and protein A Mutant strain of P1 defective in FnBPs and protein A, complemented with plasmid expressing fnbA+ Mutant strain of P1 defective in FnBPs and protein ... removed Thus, Fn and 15E11 recognize distinct determinants in FnBPA-9 Alignment of repeats and 10 of both FnBPA and FnBPB showed almost complete identity of their ﬁrst 10 amino acids, FEBS Journal ... region of FnBPB shows functional organization and immunological features of the homologous domain of FnBPA Second, the epitopes recognized by 15E11 are localized to repeats and 10 of both FnBPA and...

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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

Ngày tải lên : 07/03/2014, 00:20

... refolding of misfolded proteins [5–8] and remove protein aggregation [9–12] Mechanisms of protein osmolyte interactions, the effect of osmolytes on protein stability, and how osmolytes correct protein ... presence of these amino acids and amino acid derivatives, and values of DGD°, measured in triplicate, are given in Table The effect of polyols on the kinetic parameters (Km and kcat) of the RNase-A ... effects that urea has on protein function [16–19] Despite signiﬁcant advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the relationship...

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Báo cáo khoa học: Amino acids Thr56 and Thr58 are not essential for elongation factor 2 function in yeast potx

Ngày tải lên : 07/03/2014, 05:20

... function of speciﬁc amino acids in bacterial EFG [26–29] To date, there are only a few reports in which this technique has been used to acquire information on the importance of speciﬁc amino acids and ... position of T56 and T58 effects on the proportion of viable cells and on the doubling time Double mutant T56M ⁄ T58W was fully functional and the growth rate of cells expressing this variant of eEF2 ... base without amino acids and agar were from BD (Franklin Lakes, NJ, USA) Ammonium sulphate and amino acids were from Merck (Darmstadt, Germany) Strains, plasmids and primers The strains and plasmids...

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Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc

Ngày tải lên : 07/03/2014, 11:20

... coupling of M2 with GOA-1 We prepared an M2 mutant::GOA-1 fusion protein and directly assessed the muscarinic-ligand-dependent activation of GOA-1 Results Expression of M2::GOA-1 fusion protein ... results indicate that M2 in the GOA-1 fusion protein is functional, and the ligand-binding properties agree with that of the Gai1 fusion protein Activation of GOA-1 by muscarinic agonists Agonist-bound ... western blot analysis and well transferred in the membrane fraction Binding properties of muscarinic ligands with M2::GOA-1 fusion protein The binding properties of muscarinic ligands were examined...

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