... ve-carbon amino acids) and asparagine and aspartate (four-carbon amino acids) (up to 80% of the total amino acids) (Fig 3) 4068 te te cc mc se Fig In situ hybridization of the transcripts of GLU1, ... biosynthesis and transport ofaminoacids under photorespiratory and nonphotorespiratory conditions Results Expression of the genes for GOGATs and CPSase In order to understand the physiological role of ... entry of excess ammonium and incoming primary nitrogen into glutamine and glutamate (Fig 8) These aminoacids are trafcked under the ne control ofamino acid transporters [32,33] Active uptake...
... stimulus-secretion coupling ofamino acid-induced insulin release: insulinotropic action of br anched-chain aminoacids at physiological concentr ations ofglucoseand glutamine Eur J Clin Invest ... Nishitani S Takehana K: Phar macological activities of br anched-chain amino acids: augmentation of albumin synthesis in liver and improvement ofglucose metabolism in skeletal muscle Hepatol Res ... match The effect of branched-chain aminoacidsand arginine on glucose disposal and performance was also investigated Nine well-trained male wrestlers participated in trials in a random order Each...
... introduction of the technology, various methods were searched for and developed for microbial production of other aminoacids Today a whole array ofaminoacids are produced by microbial methods and used ... Brevibacterium lactofermentum, and B flavum in the presence of high concentrations of sugar and ammonium ions at neutral pH and under aerobic condition [2] The pathway of biosynthesis of l-lysine and l-threonine ... soybea1ns contain lower amounts of lysine, which is one of Microbial Production ofAminoAcids in Japan 77 the essential aminoacids for those livestocks The estimated amount of l-lysine produced in...
... examine the effect of the combination ofaminoacids at positions and on protein N-myristoylation, sequential vertical-scanning mutagenesis of the aminoacids at positions and in a model N-terminal ... combination ofaminoacids at positions and might be a critical determinant for protein Nmyristoylation In the present study, to examine the effect of the combination ofaminoacids at positions and ... addition to the combination ofaminoacids at positions and 6, it was demonstrated that the combination ofaminoacids at positions and also affects the susceptibility of the protein to protein...
... half-lives of plasmin in these experiments (also before 50% of the plasmin activity was inhibited) Computer model of the C-terminal 40 aminoacids in antiplasmin A computer model of the C-terminal 40 amino ... hydrophobic amino acid distant from the C-terminal portion of antiplasmin seems therefore to be of little importance Expression and purification of antiplasmin variants wt-antiplasmin and seven ... added to a mixture of plasmin substrate, 6-aminohexanoic acid and anti-antiplasmin IgG as described in Material and methods After incubation for 90 at room temperature and addition of acetic acid,...
... atom and racemisation of ␣ -amino acids 4.4.7 Reactions of the amide group in acylamino acidsand peptides Derivatisation ofaminoacids for analysis 4.5.1 Preparation of N-acylamino acid esters and ... Chromatographic and related methods for the separation of mixtures ofamino acids, mixtures of peptides and mixtures ofaminoacidsand peptides 4.14 Separation of amino- acid and peptide mixtures ... Sources and roles ofaminoacidsand peptides 1.2 Definitions 1.3 ‘Protein aminoacids , alias ‘the coded aminoacids 1.4 Nomenclature for ‘the protein aminoacids , alias ‘the coded aminoacids ...
... contributions MB and CD developed the original study concept and protocol MB, CD, and LB were responsible for acquisition and analysis of the data; development of the initial draft manuscript, and manuscript ... for the interpretation of the data; review of the draft versions of the manuscript; provision of feedback for important intellectual revisions; and review and final approval of the version to be ... updates and recommendations provide a valuable source of information to clinicians, policy makers, and researchers internationally who have an interest and mandate to advance quality of cancer...
... ΔSO2 and Δ[Lac] may be of little clinical interest if these gradients are produced exclusively by mixing of SVC and IVC blood streams On the other hand, if ΔSO2 and Δ[Lac] result from mixing of ... heart is the most aerobic of organs, normally deriving its energy from the oxidation of free fatty acidsand lactate, and coronary venous blood normally has the lowest SO2 of any venous blood [7] ... exclusively from mixing of SVC and IVC blood streams, we measured concentrations of oxygen and [Lac] in the central veins, the right heart chambers, and the pulmonary artery of hemodynamically stable...
... TOWARDS THE SYNTHESIS OF -AMINO ACIDS PAN YUANHANG (BSc., Zhejiang University) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMISTRY NATIONAL UNIVERSITY OF SINGAPORE To ... Mannich reactions of fluorocarbon nucleophiles VIII List of Figures Figure 1.1 Structures of compounds 46 and 47 Figure 1.2 Monitoring of the reaction in DMF-d7 as a function of time at room temperature ... consumption of 82 was incomplete Due to the similar polarity of 82 and 83, it was difficult to separate them Because of these two facts, the yield of 83 was about 30-70% 30 Chapter 2.2.2 Synthesis of...
... equation using a Km of 460 lM, a Vmax of 2.52 nmol PiÆminÆmg)1 and an offset of 0.1 nmol PiÆminÆmg)1 for SUR2A, and a Km of 41 lM, a Vmax of 0.73nmol PiÆminÆmg)1 and an offset of 0.05 nmol PiÆminÆmg)1 ... values of 995 and 878 lM, and Vmax values of 31 and 27 nmol PiÆmin)1Æmg)1 protein, respectively The dashed line is the average of the ATPase activities • of NBD1 and NBD2B Table ATPase activities and ... lm), and NBD2A (529 lm) The different activities of NBD2A and NBD2B could result from an inhibitory effect of the C-terminal 42 aminoacidsof NBD2B or a stimulatory effect of the equivalent amino...
... region (amino acids 19–43), and four extracellular domains spanning aminoacids 44–150 (domain C), 151– 274 (domain D), 275–586 (domain E), and 587–750 (domain F) While the domain spanning aminoacids ... the putative zinc ligands, putative substrate-binding residues and other aminoacids situated in the vicinity of these residues The results confirmed the importance of the amino acid residues, ... chromatography and chromatofocusing, as described previously [21] Results Expression and secretion of truncated variants of GCPII To analyze the contribution of individual domains of human GCPII...
... uptake because mutations in these aminoacids caused lack of transport; the aminoacids in question are Asp115, Glu257, and Cys266 All of these are conserved in SBATs and are found also in the human ... importance of negatively charged aminoacids for sodium-coupled substrate cotransport or exchange [12,13,16–18] Mutations of all conserved and two nonconserved negatively charged aminoacids to ... Asp115Asn and Glu257Gln are also given in a Hill plot Fig The relationship between the amount of injected cRNA and taurocholate uptake into X laevis oocytes via Ntcp and Ntcp mutants Uptakeof [3H]taurocholate...
... Results of the yeast protein interaction assay are shown in Fig 8(B) Substitution ofaminoacids 23, 26 and 27 completely abolished interaction with GalDBD-AR.LBD and alanines at positions 24 and ... function of AR NTD and thus does not allow discrimination between loss of AR.NTD-AR.LBD binding and loss of AR.NTD transactivating function In the yeast assay, loss of transactivation function of AR ... results are presented in Fig In the absence of ligand, none of the AR NTD proteins showed LBD interaction However, in the presence of ligand, both AR.NTDwt and AR.NTDD3– 13 bound to AR LBD with similar...
... aromatic aminoacids (Phe, Tyr, and Trp); negatively charged aminoacids (Asp and Glu); positively charged aminoacids (Lys and Arg); small aliphatic aminoacids (Gly and Ala); larger aliphatic amino ... aminoacids (Val, Leu, Ile, and Pro); and hydrophilic aminoacids (Ser, Thr, His, Asn, Gln, Cys, and Met) The nomenclature of Schechter and Berger [5] was adopted to designate the aminoacids ... Arg143 and Lys192 are essential in conferring a strong preference for acidic aminoacids at position P2¢ of the substrates Table P2¢ specificity andaminoacids found in positions 40, 143 and 192 of...
... Comparisons of relative enzymatic activities of CpcE ⁄ F and their mutants, of covalent binding of PCB to CpcE and CpcF, andof their capacity for transferring PCB All test were done under standard ... CpcE(L276D) Fig (A, B) Comparison of conserved N- and C-terminal domains in CpcE and PecE from different organisms, and (C) of N-terminal aminoacids in CpcF and PecF CLUSTAL W (1.8) multiple ... amounts of CPC [20,25,26] Homologous lyases of the ‘E ⁄ F-type’ are involved in the attachment of PEB and PCB to C84 Chromophore binding by a-phycocyanin lyase of the a-subunits of CPE and PEC,...
... Figures xiv List of Tables xvi List of Abbreviations Chapter One Introduction An Overview of Pain, its physiology, classification and molecular mechanisms of pain, role ofaminoacidsand known peptides ... concentration of pain related aminoacids 79 3.3 CSF concentration of other aminoacids not related to pain 81 Chapter 4.1 Comparison of concentration of pain-related aminoacids in CSF Acute labor ... Three An analysis ofamino acid neurotransmitters and nitric oxide in acute labor pain Applying our new method for amino acid analysis in CSF to analysis of physiological aminoacidsand other pain...
... localized on chromosome 10q24 and consists of 32 exons encoding a polypeptide of 1545 aminoacids The membrane topology for MRP2 is like that of MRP1 and consists of 17 TM helices, which form ... chromosome 17q21.3 and contains a single ORF of 1527 amino acids, with a predicted molecular mass of 169.4 kDa The membrane topology predicted for MRP3 is like that of MRP1 and MRP2 and comprises ... recognition and transport activity have been identified A series of studies have shown that mutation of individual aromatic, polar, and charged aminoacids can affect the recognition and transport of...
... Gomurashvili, Z., Katsarava, R., and Kricheldorf, H.R (2000) Amino acid based bioanalogous polymers Synthesis and study of new poly(ester amide)s composed of hydrophobic α -amino acidsand dianhydrohexitoles ... C.C., and Won, C.Y (1999) Amino acid-based bioanalogous polymers Synthesis, and study of regular poly(ester amides) based on bis(α -amino acid) α,ω-alkylene diesters, and aliphatic dicarboxylic acids ... S.N., Yarmush, M.L., and Kohn, J (1993) Copolymers of lysine and polyethylene glycol: a new family of 129 130 Biodegradable Polymers Composed of Naturally Occurring α -Amino Acids 51 52 53 54 55...
... control of SMC proliferation 4466 S Bousserouel et al (Eur J Biochem 271) Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the ... Modulation of smooth muscle cell (SMC) proliferation and retinoblastoma protein (Rb) phosphorylation after supplementation with n-3 and n-6 polyunsaturated fatty acids (PUFAs) (A,B) Effects of n-3 and ... Rb and Egr-1, in order to determine how the incorporation of EPA and DHA modulate SMC proliferation This article describes the differing effects of n-3 and n-6 PUFAs on the proliferation of SMC...
... side chain of any bound amino acid The lability of the a-proton observed for a large number ofaminoacids [5] under the action of TPL gives evidence for the orthogonal orientation of the a-proton ... with respect to the cofactor plane [17], and shows that the pattern of binding is the same for a variety ofaminoacids It has been established [5] that for a number ofamino acid inhibitors ... Center (TW00106) to R.S.P and T.V.D References Konnikova, A.S., Dobbert, N.N & Braunstein, A.E (1947) Labilization of a-hydrogen ofaminoacids under the action of aminoferase Biokhimia 12, 556–568...