... staining was performed: carbol fuchsin 0.3%, slow heating until steaming, rinsing after 5 min, destaining with 25% sulfuric acid, rinsing, counterstaining with methylene blue 0.3% for 1 min, ... microscopic detection of tuberculosis. Eur J Clin Microbiol Infect Dis 2008; 27: 849–855. 6 Mase S R, Ramsay A, Ng V, et al. Yield of serial sputum speci-men examinations in the diagnosis of pulmonary ... signi cantly increase the yield of sputum smear microscopy for the detection of pulmonary TB in Laos.KEY WORDS: tuberculosis; case detection; microscopy; sputum; sodium hypochloriteTUBERCULOSIS...