detection of calcium sparks in intact and permeabilized skeletal muscle fibers

Evaluation of loop mediated isothermal DNA aplification (LAMP) detection of salmonella spp in foods and listeria monocytogenes on environmental surfaces

Ngày tải lên : 30/09/2015, 10:11

... to grow and make consumers sick Hence, monitoring of contamination in the food is crucial Advances in detection methods of pathogens in foods and environmental samples using molecular detection ... are trading of contaminated foods between countries/states which increases the likelihood of outbreak and illness coupled with changes in lifestyle and consumer demands such as increasing consumption ... trays and food scoops were used across raw and cooked foods resulting in cross contamination As for foods prepared by caterers, the absence of soap on the premises and handling of food by the handlers...

82
415
0

Detection of Antiviral Drugs Oseltamivir Phosphate and Oseltamivir Carboxylate in Neya River, Osaka, Japan

Ngày tải lên : 05/09/2013, 10:15

... expected in surface water bodies, especially those receiving effluents from sewage treatment plants, during the winter season of 2009/2010 in Japan owing to H1N1 influenza pandemic and drastic increase ... prepared in methanol and stored in refrigerator (4ºC) prior to usage Description of Sampling Sites Fig shows the location of the sampling sites with the map of Japan inset The Neya River is located in ... obtained from Osaka Prefectural Institute of Public Health (2010) An index value gives the total number of influenza cases (average) in a hospital in a week The - 366 - Journal of Water and Environment...

10
381
0

Báo cáo khoa học: Casein phosphopeptide promotion of calcium uptake in HT-29 cells ) relationship between biological activity and supramolecular structure ppt

Ngày tải lên : 16/03/2014, 05:20

... than 10 min, including a 1–2 interval between the addition of Ca2+ and that of CPP In this period of time, cells maintained full viability As a control, [Ca2+]i concentrations after ionomycin treatment ... absorption of calcium by casein phosphopeptides in rachitis and normal chicks J Nutr 119, 2141–2148 25 Bronner F, Pansu D & Stein WD (1986) Analysis of intestinal calcium transport across the intestine ... Intestinal absorption of calcium in rats given diets containing casein or aminoacid mixture: the role of casein phosphopeptides Br J Nutr 49, 67–76 38 Sato R, Noguchi T & Naito H (1986) Casein...

13
468
0

báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc

Ngày tải lên : 18/06/2014, 15:20

... meaningful While the validation of these three T cell assays was challenging, the experience we obtained during validation studies and conducting patient screening will assist us and others in ... Table 10) Use of validated assays in clinical patient monitoring minimized assay reproducibility problems and allowed better interpretation of clinical data http://www.translational-medicine.com/content/6/1/61 ... 2–8°C) was also evaluated and RT was chosen Co-staining with anti-CD3 showed decrease tetramer binding probably due to proximity of CD3 and TCR, therefore anti-CD3 staining was not used Fixed cells...

25
639
0

Báo cáo hóa học: " Detection of epithelial apoptosis in pelvic ileal pouches for ulcerative colitis and familial adenomatous polyposis" pot

Ngày tải lên : 18/06/2014, 16:20

... inhibition of insulin receptor substrate-1 expression leads to insulin resistence, hyperinsulinemia, and increased adiposity Endocrinology 2005, 146:1428-1437 Hsu SM, Raine L, Fanger H: Use of Avidin-Biotin-Peroxidase ... activity index Mayo Clin Proc 1994, 69:409-415 Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal ... expression of pro-apoptotic and anti-apoptotic proteins of Bcl-2 [31,32] and Caspases [33,34] families to evaluate intrinsic and extrinsic pathways of apoptosis in normal ileal pouches Even in such...

6
407
0

Báo cáo sinh học: "Detection and characterization of translational research in cancer and cardiovascular medicine"Ơ docx

Ngày tải lên : 18/06/2014, 19:20

... sole clinical mix journal in the set, maintains links to both the clinical domain and the molecular biology domain This journal plays a key role linking diverse research interests while 267 of the ... in each set of specialty journals Here, instead of selecting and mapping some portion of the most specific links, we mapped only the links between the nearest nodes, as follows The strength of ... descriptions of widely used methods and techniques One interesting set are the guidelines and criteria of various sorts seen in both the cancer plot (Boland 1998, Mountain 1997, Therasse 2000, Sobin 2002)...

12
528
0

báo cáo hóa học:" Detection and characterization of translational research in cancer and cardiovascular medicine" potx

Ngày tải lên : 20/06/2014, 03:20

... sole clinical mix journal in the set, maintains links to both the clinical domain and the molecular biology domain This journal plays a key role linking diverse research interests while 267 of the ... in each set of specialty journals Here, instead of selecting and mapping some portion of the most specific links, we mapped only the links between the nearest nodes, as follows The strength of ... descriptions of widely used methods and techniques One interesting set are the guidelines and criteria of various sorts seen in both the cancer plot (Boland 1998, Mountain 1997, Therasse 2000, Sobin 2002)...

12
397
0

Báo cáo khoa học: "Immunohistochemical detection of Prion protein (PrP-Sc) and epidemiological study of BSE in Korea" pot

Ngày tải lên : 07/08/2014, 14:23

... traumatic injuries 4.3%, and unknown causes 3.7% H&E and amyloid staining The H&E staining of brain stem and mesencephalon from bovine brain obtained from Korean slaughterhouses between 1996 and 1999 ... technique described in this report is suitable for the assay of bovine brain, retina, distal ileum, deer or elk brain, and of ovine brain, spleen and lymphoid tissues, including the peripheral ... farms A total of 140 bovine brains were collected from slaughterhouses in Korea from 1996 to 1999 After fixation in 10% buffered formalin and embedding in paraffin, blocks of brain tissue from...

7
266
0

Báo cáo khoa học: "Simultaneous detection of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in swine intestinal specimens by multiplex polymerase chain reaction" ppsx

Ngày tải lên : 07/08/2014, 18:21

... smsinagro cinegohtap lareves fo noitacifitnedi evitinifed eht rof detroper neeb sah )RCP( noitcaer niahc esaremylop fo esU msinagro cinegohtap fo tset citsongaid dipar erom fo tnempoleved eht rof dewolla ... dna siralullecartni L fo noitceted rof RCP xelpitlum fo yticificepS giF AND fo gp 05 ;5 enaL ,AND fo gp 001 ;4 enaL ,AND fo gn ;3 enaL ,AND fo gn 01 ;2 enaL ,AND etalpmet fo gn 001 ;1 enaL ,III ... 67031CCTA AND siralullecartni.L *SQRVN secruoS sniarts lairetcaB secruos dna )AND( sniarts lairetcab fo tsiL elba T RCP fo ecneserp ehT nim rof C 27 ta noitaziremylop lanoitidda yb dewollof ,nim rof...

7
301
0

Báo cáo lâm nghiệp: "Detection of cavitation events upon freezing and of water in stems using ultrasound technique" pdf

Ngày tải lên : 09/08/2014, 04:20

... assess that, together with examining the modifications in water uptake and water conductivity during freezing and after thawing References Hammel H.T (1967) Freezing of xylem sap without cavitation ... behavior of ultrasound emissions in Araucaria subjected to the same kind of test As in Eucalyptus, only a few single events were recorded during supercooling, but the highest number of acoustic ... insulated box to prevent soil freezing The cut extremity of Auracaria branches, recut under water, was kept immersed in water and enclosed in an insulated box Plant stems were frozen in a...

4
229
0

Báo cáo y học: "Detection of bone erosions in rheumatoid arthritis wrist joints with magnetic resonance imaging, computed tomography and radiography" ppt

Ngày tải lên : 09/08/2014, 10:23

... difference and sensitivity to change of magnetic resonance imaging and radiographic scoring of structural joint damage in rheumatoid arthritis finger, wrist, and toe joints: a comparison of the OMERACT ... evaluation of magnetic resonance images, and was involved in patient recruitment MH was involved in the CT scanning protocol EN performed the evaluation of radiographs JM was involved in the MRI scanning ... after proper training and calibration of readers, appears suitable for use in monitoring joint inflammation and destruction in RA [36] The close correlation with erosion volumes determined by MRI,...

8
372
0

Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps

Ngày tải lên : 09/08/2014, 14:22

... positive in 20 of the 27 (74.10%) patients indicating the presence of bacterial 16S rDNA within the synovial samples Of note, PCR was positive in five out of five (100%) patients with ReA and also in ... arthritis fied in three out of seven (43%) RA patients and in three of six (50%) OA patients Accordingly, the presence of bacterial DNA within the SF samples was significantly higher in ReA and UA patients ... risk of triggering an immune response finally culminating in synovitis However, genetic susceptibility factors of the host are also playing an important role particularly in persistent infections...

11
461
0

Báo cáo y học: " Detection of HIV-1 RNA/DNA and CD4 mRNA in feces and urine from chronic HIV-1 infected subjects with and without anti-retroviral therapy" pptx

Ngày tải lên : 10/08/2014, 05:21

... contained in the feces could reveal the degree of pathogenesis in GALT Detection of HIV-1 has been reported in fecal specimens from drug naïve HIV-1 infected individuals in the acute phase of infection ... in GALT, HIV-1 and CD4+ T cells shed into the intestinal lumen and the amount of HIV-1 and CD4+ T cells in the feces would associate with the pathogenic changes in GALT Since the components of ... to intestinal lumen might be higher compared to the later chronic phase of infection The gastrointestinal tract is the major reservoir of viral infected cells and the site of rapid and profound...

11
336
0

báo cáo khoa học: "Journal of Hematology & Oncology BioMed Central Short report Open Access Detection of NPM1 exon 12 mutations and FLT3 – internal tandem duplications by high resolution melting analysis in normal karyotype acute myeloid leukemia" docx

Ngày tải lên : 10/08/2014, 22:20

... Ronnstrand L: Identification of Y589 and Y599 in the juxtamembrane domain of Flt3 as ligand-induced autophosphorylation sites involved in binding of Src family kinases and the protein tyrosine phosphatase ... double stranded DNA [11] As melting is sequence dependent, monitoring the precise melting behaviour by observing the change in fluorescence allows the detection of variant sequences In addition, ... detected in the sequence; numbering according to NPM1 reference sequence NM_002520.5 | Numbering according to FLT3 reference sequence NM_004119.2 (5'UTR not included) ¶ The size of the internal tandem...

5
236
0

Báo cáo khoa học: " A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification" pps

Ngày tải lên : 12/08/2014, 04:21

... be applied routinely in order to conduct timely survey on GPV infection in goose farming In doing so, the virus-carrying goslings can be identified during the early stages of infection; countermeasures ... detection of GPV infection in goslings Optimal LAMP assay was evaluated by analyzing GPV infected gosling tissues DNA extracted by the tissue Figure Determination of the optimal temperature and ... tool for the detection of GPV infection in both laboratory and field settings Materials and methods Goslings, tissues, virus, DNA, and standard plasmid DNA templates preparation Goslings, tissues,...

7
382
0

Báo cáo khoa học: "Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes" ppt

Ngày tải lên : 12/08/2014, 04:21

... nucleotide sequence of the CP gene and the amino acid sequence of coat protein showed that all PVM isolates fell into two distinct groups - I and II (Table and Fig 3) PVM isolates in group I only ... China, Poland and Russia and group II consisted of Canadian and US isolates Isolates in group I might be further divided into two sub-groups - Ia, and Ib PVM isolates from China and Poland formed ... primers PVM3 and PVM4, a NcoI site was revealed in PVM isolates detected in Germany, Italy, Russia, Poland and China, but not found in the isolates detected in the US (Idaho strain) and Canada...

7
452
0

Báo cáo y học: "Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after crosslinking of the high affinity IgE receptor FcεRI" ppsx

Ngày tải lên : 13/08/2014, 13:22

... allergen Probability binning offers an approach that combines CD63 and CD203c into one metric that has a high response A combination of lysis with Saponin and the markers CD203c and CD63 [17] computed ... basophils and control cells Histograms of CD63 and CD203c expression on basophils and control cells Histograms of expression of CD203c (a & c) and CD63 (b & d) on basophils at baseline (red line) and ... Immunoprep/Q-prep) and non responders (n = 5) Probability binning offers an integrative alternative to using either only CD203c or only CD63 When analyzing the same dataset by defining that the probability binning...

9
392
0

Báo cáo sinh học: " Detection of multiple quantitative trait loci and their pleiotropic effects in outbred pig populations" pptx

Ngày tải lên : 14/08/2014, 13:21

... Theory of Statistics London: Edward Arnold; 1991 Lund MS, Sorensen P, Guldbrandtsen B, Sorensen DA: Multitrait fine mapping of quantitative traits loci using combined linkage disequilibria and linkage ... estimated using the mapping software Crimap [26] Model and test statistic Mixed model Our model includes sex as a fixed effect and polygenic and QTL genotypic effects as random effects Random effects ... QTL in the first position but no QTL in the second position H2: QTLs in both first and second positions When comparing the hypothesis of one QTL in the second position with that of QTLs in both...

11
315
0

Experimentation, modeling and control of calcium dynamics in human vascular endothelial cells

Ngày tải lên : 09/09/2015, 18:48

... identifying the structure of the mechano-sensor in VECs membrane, ﬁnding signaling pathway given certain type of mechanical stimulus and investigating the interplay of gene expression and cell ... biological/physiological and engineering perspectives Proﬁcient skills in handling cells and chip In our case, we need to integrate cell culture, ATP release measurement and Ca2+ imaging into the one system The main ... concentration of ATP on cell surface, in uencing calcium mobilization in an indirect fashion In the meanwhile, there were several other groups providing their results supporting Ando’s point of view...

96
259
0

Analysis and detection of human motion in time frequency domain

Ngày tải lên : 14/09/2015, 18:27

... down, lying to sitting, level walking, ascending stairs and descending stairs However, in sitting, standing, and lying down detection, instead of detecting static postures, we detect stand-sit/sit-stand, ... thigh in detecting sitting, standing, and lying static activities The dc response of the radial sternum accelerometer was used to distinguish sitting/standing posture from lying posture and that of ... consisting of seven types of activities, sitting, standing, lying supine, level walking, ascending stairs, descending stairs, and cycling The recorded acceleration signals were manually separated in...

167
289
0