detection of mycobacterium tuberculosis in respiratory specimens by using the gen probe amplified mycobacterium tuberculosis direct test

Báo cáo khoa học: "Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR" pot

Ngày tải lên : 07/08/2014, 14:22

... present in the porcine intestine and feces including swine genomic DNA The number of amplification cycles was one of the important factors for increasing the sensitivity of the PCR assay The increase ... methods include H E staining and silver staining of the intestinal sections 36 Dong-Kyun Suh et al Fig Section of the ileum from a pig infected with L intracellularis a : Presence of numerous intracellular, ... (arrow) in the apical cytoplasm of the crypt epithelial cells, Warthin-Starry staining, 1,000 b : The crypts are filled with inflammatory cells, H&E staining, 400 organisms were found in the...

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Báo cáo khoa học: " Detection of Babesia divergens in southern Norway by using an immunofluorescence antibody test in cow sera" docx

Ngày tải lên : 12/08/2014, 18:22

... possible cause of the decline in incidence since the 1930 s is changes in the use of pastures Changes in legislation leading to increased use of wood pasturing may reverse the decline Hasle et ... before the study, indicating that there is a substantial risk of babesiosis in their present locality The presence of B divergens was confirmed by IFAT in a total of 17 of the 24 farms we tested ... present in their pasturing areas The screening of cows for B divergens infection would therefore be expected to be a sensitive method for detecting the presence of the parasite in a locality, if testing...

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Báo cáo y học: "Effective detection of rare variants in pooled DNA samples using Cross-pool tailcurve analysis" ppsx

Ngày tải lên : 09/08/2014, 23:20

... reflected in the highest MCC score of all the tested methods (84.2%) Taken together, the combination of SERVIC4E with either base-calling algorithm provides the highest combination of sensitivity ... present in only a single pool) Cross-pool filtering using SERVIC4E Further validation by Sanger sequencing indicated the persistence of a few false positive calls from this dataset Analysis of these ... defined as the ratio of the sum of Phred quality scores of the variant base call to the sum of Phred quality scores of all base calls; (3) average quality, defined as the average quality of all...

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Báo cáo y học: "Continuous noninvasive monitoring of barbiturate coma in critically ill children using the Bispectral™ index monitor" doc

Ngày tải lên : 13/08/2014, 08:20

... awake) In addition, the EEG recorded by the BIS is continuously displayed (BIS-EEG), together with the device's estimate of the SR The SR calculated by the BIS (SR-BIS) represents the percentage of ... Guidelines for the acute medical management of severe traumatic brain injury in infants, children, and adolescents Chapter 13 The use of barbiturates in the control of intracranial hypertension in ... of the study and in the interpretation of the data JHB participated in the interpretation of the data and helped to draft the manuscript DT conceived the study and participated in its design GHV...

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R"eCseoarcuh garthicl eofficer screening" improves detection of pulmonary tuberculosis in hospital in-patients pptx

Ngày tải lên : 06/03/2014, 04:20

... admitted to the ICU and paediatrics departments after completion of Stage I By excluding the TB patients in the ICU, we found that 81% and 100% of TB patients in the Internal Medicine Department in Stage ... difference might due to the increased awareness of TB in Stage II (Figure 2), or because of interference of TB diagnosis by other severe symptoms among patients in the ICU during Stage I In fact, our COS ... Role of Cough Officer Screening in Early Detection of Pulmonary Tuberculosis in Inpatients Thorac Med 2006, 21:321-327 Page of 13 Granich R, Binkin NJ, Jarvis WR, Simone PM, Rieder HL, Espinal...

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The bleach method improves the detection of pulmonary tuberculosis in Laos ppt

Ngày tải lên : 29/03/2014, 03:20

... staining was performed: carbol fuchsin 0.3%, slow heating until steaming, rinsing after min, destaining with 25% sulfuric acid, rinsing, counterstaining with methylene blue 0.3% for min, rinsing ... conservation of the NaOCl solution; the incubation time; the power and duration of centrifugation (or duration of sedimentation); blinded reading; the minimum duration of smear reading; and the positivity ... to the distance of the target populations from the health centres: the risk of contamination by sample handling and the long delays between sputum collection and smear staining The addition of...

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Báo cáo khoa học: "Time of initial detection of fetal and extra-fetal structures by ultrasonographic examination in Miniature Schnauzer bitches" ppsx

Ngày tải lên : 07/08/2014, 23:22

... minA llamS J stnemerusaem latef cihpargonosartlu gnisu sehctib ezis llams dna muidem ni ega lanoitatseg fo noitanimreteD A inoirG ,CG inovuL 31 23-72 ,14 ,6002 minA tsemoD dorpeR ycnangerp eninac ... -103 ,8 ,7691 tcarP minA llamS J ygolohprom lacigolotyc dna euqinhceT I ygolotyc lanigav eninaC PA ettuhcS 71 04-53 392 ,33 ,2991 dnuosartlU loidaR teV suretu mutraptsop eninac eht fo yhpargoidar ... serutcurts latef fo noitceted laitini fo emit ehT )82 yaD ,1 giF( oyrbme eht dessapmocne hcihw ,)92-62( 0.1 ± 7.72 yad no detceted saw ,enarbmem cinoinma eht ,enarbmem latef cinegohce ssel ,dnoces A )72-62(...

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Báo cáo khoa học: " A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples" potx

Ngày tải lên : 07/08/2014, 23:22

... respectively, which in turn indicated the ability of this method in detecting low levels of MAP organisms Moreover, with the use of the RT-PCR product, in general only viable organisms present in the feces ... with μl of synthetic DNA with different concentrations ranging from 1-1,000 fmol μ/l The limit of detection was determined using the signal obtained for the negative control plus three times the ... suit the high G + C content of the MAP genome Generally, milk and feces are considered to be the most suitable clinical specimens for the diagnosis of JD However, because of the presence of large...

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Báo cáo y học: " Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood" doc

Ngày tải lên : 12/08/2014, 04:20

... and the CT values were similar in the samples collected by the pediatrician and parents Furthermore, the direct involvement of parents significantly increased the patient's acceptance of the ... taken by a parent in four There were no differences in CT values suggesting less virus and lower sensitivity, and no differences in the timing of the collections or in the age or weight of the ... losing the sample when respiratory episodes occur Secondly, the samples can be obtained immediately after the onset of the first signs and symptoms, thus favouring the identification of the infectious...

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Báo cáo khoa học: " A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification" pps

Ngày tải lên : 12/08/2014, 04:21

... a finding confirmed by our results In other words, the specificity and the detection limit of the LAMP assay are equal with FQPCR for detecting GPV The optimal condition for detecting GPV by ... survey on GPV infection in goose farming In doing so, the virus-carrying goslings can be identified during the early stages of infection; countermeasures can be devised even before the infection ... a by- product of the reaction LAMP amplicons in the reaction tube were directly detected by the naked eye by adding 1.0 μl of a thousand-fold-diluted original SYBR Green I (Molecular Probes Inc.)...

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Báo cáo y học: "Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model" pptx

Ngày tải lên : 13/08/2014, 05:21

... HTLV-I infected cells, as well assessment of the effects of immune T cells on the development of the disease [17,18] By using this model system, we also reported the therapeutic effect of Tax-coding ... activation of 4O/1C8 by RT1AlSCTax180L may fail to induce the protective signal from AICD in the CTLs Since a syngeneic CD4+ T cell line, FPM1.BP, was able to induce the expansion of 4O1/C8 in spite of ... against the virus in a mouse system in vivo [30] Thus, it will also be necessary to assess the in vivo effect of the rat SCTs for the evaluation of the system as a therapeutic tool in HTLV-I infection...

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Tài liệu Detection of Pulmonary tuberculosis: comparing MR imaging with HRCT pptx

Ngày tải lên : 15/02/2014, 12:20

... between the level of the carina and the origin of the inferior pulmonary veins; and the lower zones as areas below the origin of the inferior pulmonary veins Each zone had approximately the same ... parenchymal finding was scored on a scoring sheet using the following sliding scale of relative certainty: = definitely negative; = probably negative; = indeterminate; = probably positive; = definitely ... classified each lung by zone: upper, middle, and lower, resulting in a total of six zones per patient The upper zones were defined as areas of the lung above the level of the carina; the middle zones...

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Tài liệu Báo cáo khoa học: "Automatic Detection of Nonreferential It in Spoken Multi-Party Dialog" doc

Ngày tải lên : 22/02/2014, 02:20

... positives The slightly higher recall of the model using the sentence boundary information is mainly due to a better coverage of the rule using the features encoding the distance to the next toinﬁnitive ... again The results reported in the following are from this second annotation We then examined the inter-annotator reliability of the annotation by calculating the κ score (Carletta, 1996) The ... recall of 55.1%, a precision of 71.9% and a resulting F-measure of 62.4% for the detection of the class nonreferential The overall classiﬁcation accuracy was 75.1% The advantage of using a machine...

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Báo cáo " Development of climate change scenarios for small areas in Vietnam by using the MAGICC/SCENGEN software in combination with statistic correction" docx

Ngày tải lên : 05/03/2014, 16:20

... consideration In the study, the temperature and rainfall scenarios are built in the monthly form of decades of the 21st century However, within the scope of this paper, we only introduce climate ... have been tested for the statistic reliability through: + Assumption testing the magnitude of the correlation coefficient Rxy + Assumption testing the magnitude of the coefficient of regression ... value in increasing level, followed by Quy Nhon and Can Tho This is quite compatible with the temperature happening in the past (increasing level declined southwards) In addition, temperatures in...

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Detection of Pseudomonas aeruginosa in sputum headspace through volatile organic compound analysis potx

Ngày tải lên : 05/03/2014, 21:20

... Steinheim, Germany) were injected using the same GC-MS method to determine the retention indices of the individual compounds using a modified Kovats method [33] Bacterial culturing Sputa were inoculated ... significant higher in vivo concentrations in most strains of P aeruginosa [18] This biomarker could then be used in the detection of P aeruginosa in breath, whether or not in combination with CH3SCN ... identify the major source of a certain VOC We tried to minimize the effect of saliva contamination by asking the patient to rinse their mouth prior to sputum production Nonetheless, finding a biomarker...

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Xpert MTB/RIF test for detection of pulmonary tuberculosis and rifampicin resistance (Protocol) pptx

Ngày tải lên : 06/03/2014, 04:20

... could not be determined Proportion of indeterminate results will be the number of tests classiﬁed as ’indeterminate’ divided by the total number of tests performed Culture contamination will be ... addressed by pre-specifying subgroups We will assess heterogeneity by visual inspection of forest plots We will further investigate potential sources of heterogeneity by adding covariates in the models ... Comparison of the Xpert MTB/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens Journal of Clinical Microbiology...

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Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot

Ngày tải lên : 17/03/2014, 10:20

... previously In the pIFNHA construct, the IFN-b gene was tagged by PCR using an oligonucleotide containing three repetitions of the sequence corresponding to the human in uenza A virus hemaglutinin (HA) ... investigated the in uence of the ARE and the CRID on the poly(A) status of the human IFN-b mRNA in natural conditions of IFN-b synthesis Materials and methods Reagents All the reagents and enzymes used in ... was inserted in the HincII site of the pIFNHA construct located at the beginning of IFN-b mRNA 5¢UTR To place the IFN-b gene under the transcriptional control of a constitutive promoter, the...

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ag doped wo3-based powder sensor for the detection of no gas in air

Ngày tải lên : 19/03/2014, 16:47

... when the temperature of the Ag catalyst is increased to 250 8C, the sensitivity of the WO3 sensor kept at 150 8C 71 increased by three times Without heating the Ag catalyst or bypassing the gas ... patterns (the lack of their long-range order and their heterogeneity may explain the poor re¯ection hump obtained in XRD) Typically, as seen in the ®gure, a region containing ca Ê 6.93 A inter-planar ... gases) containing ca 100 ppm NO balanced with air) by the ac impedance analyzer (Fig 1) The ¯ow rate of the gases was kept at 100 ml/min throughout the measurement using mass ¯ow controllers The schematic...

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Báo cáo " On the detection of gross errors in digital terrain model source data " pdf

Ngày tải lên : 22/03/2014, 12:20

... Those parameters are: ‐ The size of the moving window, i.e. the number and location of neighbor points. ‐ The interpolation technique used for estimating height of the considered points. ... inside the moving window; coefficient (4) j K V takes a value in the range from 2 to 4. j =1 where mi is the number of data points that fall inside the moving window around point ... radius (or of the minimum number of points inside the search window), the number of correctly and incorrectly detected points is decreasing. This can be explained as a ...

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DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf

Ngày tải lên : 22/03/2014, 14:20

... portion of the sample into the solvent reservoir The water sample will drain into the cartridge, and from the exit into the suction flask Maintain the packing material in the cartridge immersed in ... test The correct setting of the baseline threshold, as indicated by the presence of low intensity ions, is the next most important part of the performance test Finally, the ion abundance ranges ... Reagent Water Water in which an interference is not observed at the method detection limit of the compound of interest Prepare reagent water by passing tap water through a filter bed containing...

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