... vector,containing a 788-base pair fragment amplified from the human genomic GAPDH gene [20]. GAPDH mRNA wasutilized as a constant mRNA for control.Statistical analysis The statistical analysis of the ... Tadolini1,2, Anna Maria Posadino1,2, Bastiano Sanna1,2, Marcella Debidda1,2,Federico Bennardini2,3, Gianni Sava4and Carlo Ventura1,21Department of Biomedical Sciences, Division of Biochemistry, ... h at 55 °Cin the presence of an unlabeled antisense c-myc RNA probegenerated by transcription of the plasmid linearized withBamHI. Samples were then incubated with a combination of RNase A and...