when installing the connector connect it firmly with the cable and equip it with a heat shrink tube so as not to expose the bare core and the handle of the connector
... based upon in vitro translation of PMV gRNA in wheat germ extracts (data not shown) Each mutant was tested in foxtail millet (Setaria italica cv German R) protoplasts to examine the effects of ... cytosol Results of replication assays in protoplasts with transcripts of pKB238 and pQP94 validate the conclusion that the CP and movement-associated genes of PMV are dispensable for replication ... millet plants and assay for RNA products Subcellular fractionation and analyses ofthe PMV replicase proteins isolated from millet plants and assay for RNA products generated by the PMV RNA-dependent-RNA...
... plantar fascia strain compared to walking without an orthoses While we are not aware of another gait study that has calculated strain within the plantar fascia, the results are consistent with previous ... for the D1MT, NAV, and MCAL markers for the purpose of calculating plantar fascia strain (PFS) and medial longitudinal arch (MLA) angle values The MLA angle was calculated in a manner similar to ... from the head ofthe first metatarsal (D1MT) tothe NAV marker (Figure 4) Plantar fascia strain is a unitless measure calculated by approximating the plantar fascia as spanning between the first...
... to pass parameters toand from subroutines andto store intermediate values used in a calculation The S7-200 provides four 32-bit accumulators (AC0, AC1, AC2, and AC3) You can access the data ... the least significant or 16 bits ofthe value that is stored in the accumulator to access the accumulator as bytes or words To access the accumulator asa double word, you use all 32 bits For ... is not initialized by the S7-200 at the time of allocation and might contain any value When you pass formal parameters in a subroutine call, the values ofthe parameters being passed are placed...
... DataRelation was created are • • Cascade, meaning that changes tothe CustomerID DataColumn of customersDT are cascaded to ordersDT This is the default None, meaning that changes tothe CustomerID DataColumn ... a DataTable named customersDT There is a row in the Orders table that also has a CustomerID of J6COM A copy of this row is stored in a DataTable named ordersDT The customersDT and ordersDT DataTable ... in the customersDT and ordersDT DataTable objects and also in the Customers and Orders database tables This works as long as you use only the OrderID column in the WHERE clause ofthe Command...
... Position ofthe Fiber Spool Box Describes the appearance ofthe fiber spool box 8. 2Installing the Plate andthe Box Describes the procedure ofinstallingthe plate andthe box 8.1 Installation ... 3500 Installation Manual Installingthe Fiber Spool Box 8.2 Installingthe Plate andthe Box Purpose This procedure guides you to install the fiber spool box into the cabinet Tools /Materials Cross ... attenuators If the cabinet holds two OSN 3500 subracks, install the fiber spool box at the third hole from the bottom ofthe cabinet, more than 50 mm away from the subrack OptiX OSN 3500 Installation...
... power cables andthe management cables 4 OptiX OSN 3500 Management cable COA power cable SS62COA SS62COA Management cable Figure 1.3 Connection ofthe power cables andthe management cables 7.2.2 ... Describes the connection andthe installation ofthe DCM 7.1 Installingthe SS61COA and N1COA This section describes the connection andthe installation ofthe SS61COA The installation ofthe N1COA is ... the SS61COA Describes the connection andthe installation ofthe SS61COA and N1COA 7. 2Installing the SS62COA Describes the connection andthe installation ofthe SS62COA 7. 3Installing the DCM Describes...
... 3500 Installation Manual 6Installing theCable Distribution Plate cabinet Figure 1.1 Position for mounting ears Cable distribution plate Installation position Cable distribution plate for the lower ... the contents of this chapter Section Description 6.1Installation Position Describes the position ofthecable distribution plate in the cabinet 6. 2Installing theCable Distribution Plate in the ... chapter guides you to install thecable distribution plate One cable distribution plate is delivered with one subrack Thecable distribution plate is installed over the subrack The following table...
... exchange is either acid-catalyzed or base-catalyzed [20] Asa consequence, the dependence of Log(kexch) (the logarithm ofthe exchange rate) asa function ofthe pH is a chevron plot witha minimum ... initiates the translocation ofthe catalytic domain Here, the data allowed identification ofthecoreofthe protein in the N state andthe evolution ofthe overall structure ofthe protein in the ... the T domain was quite similar to that ofthe dead volume (Fig 7A) According toa general estimation, the oligomers formed at pH 4.0 are at least 10-mers with an apparent molar weight of around...
... concentration of AT andthe rates of interaction in the presence and absence of heparin pentasaccharide (Table 1), one can calculate that the half-life of enzyme activity in the absence of heparin ... of AT withand without the heparin pentasaccharide bound, heparin cofactor II and cleaved protein C inhibitor are shown as indicated In all ofthe structures, theA b-sheet is shown in red, the ... physiological activator of HC-II has been assumed to be extravascular dermatan sulfate [56–64], which would complement the intravascular effect of heparan sulfate binding to AT Maimone and Tollefsen...
... air, Walls triggered his atom-pistol The crackling blast of force tore into the body ofthe charging asteroid-cat, andthe beast fell heavily a few yards away But asit fell, the small gray mass ... Kenniston andthe Jovian obeyed The Sunsprite was lying sharply canted on its side, andit was difficult to scramble down through the tilted passageways and decks tothe big main cabin The cabin was a ... long amid the wild asteroids and moons ofthe outer planets It was worse up in the glittering cocktail room atop the hotel The place had glassite walls and ceiling, and was designed to give an...
... primers TEHA1: ACACAGATCTCTGCAGGGCACCCCAGGCTTTACA and TEHA2: ACACCCATGGAGCTTTCCTGTGTGAAATTGT, lacUV5 was amplified TEHA3: ACACAGATCTCTGCAGTGAAATGAGCTGTTGACAATTA and TEHA4: ACACCCATGGTCTGTTTCCTGTG ... blotting analyses and compared withthe data obtained when analyzing the same protein in vitro Because eGFP does affect the solubility, the solubility class used in this case is the one with eGFP As ... As shown in Table 1, eGFP generally decreases the solubility of proteins belonging to classes witha high solubility and increases the solubility of proteins belonging to classes witha low solubility...
... for the prime divisors of n to lie all above their expected values An analogy from probability theory is to ask for the likelihood that a random walk on the real numbers, with each step haveing ... of Mathematics and Informatics, Bulgarian Academy of Sciences Finally, the author acknowledges the referee for a thorough reading ofthe paper and for helpful suggestions This work was partially ... (7.2) write a = a1 a2 with pβ , a1 = pβ a, β≥2 so that (a1 , a2 ) = and a2 is square-free By Lemma 3.1, L (a; σ) ≤ τ (a1 )L (a2 ; σ) ≤ στ (a1 )τ (a2 ) (7.6) Also (7.7) a1 τ (a1 ) a1 >w −3/4 a1 w−1/4...
... 5¢-aataatccatgg gggcacagcttttatatgagcgcggag-3¢ and 5¢-aataatggatcctcactgttc cttatccgatttttcgaagtgc-3¢ PCR products were doubly digested with NcoI and BamHI (New England Biolabs, Beverly, MA, USA), ... form critical intra- and intermolecular contacts These observations demonstrate the capacity, and on occasion the necessity, of TPRs to participate at all levels of structure organization, and suggest ... orthogonal tothe diagonal (helix A interacting with helix B), then parallel tothe diagonal (helix A interacting with helix Aofthe next repeat), and finally returning tothe diagonal (helix...
... that the mechanism of release ofthe axial ligand under alkaline conditions and in the presence of GdmHCl is different Under alkaline conditions release ofthe axial ligand is postulated to arise ... sensitive tothe chemical nature ofthe axial ligands tothe hemeiron [41] Upon replacing the native Met ligand with either an exogenous or protein-based ligand a change in the distribution ofthe ... for data collection and refinement are summarized in Table The program procheck [34] was used to analyse conformational variations from the defined norms, withthe quality ofthe Ramachandran plots...
... SUMA software: subsite mapping of amylases This software calculates the apparent binding energies on the basis ofthe measured bond cleavage frequencies The calculations are based on the equation: ... been made to use this program for subsite mapping of other a- amylases found in the literature Evaluations of subsite maps of rice and barley a- amylases are thus also presented MATERIALS AND METHODS ... data of Table Fig Subsite maps for porcine pancreatic a- amylase (PPA) The solid bars are related to CNP-modified maltooligosaccharide substrates [8] andthe open bars depict the subsite map with...
... twice andthe purity assessed by a MALDI-TOF analysis using a Hewlett Packard G202 5A LD-TOF system mass spectrometer and a- cyano-4-hydroxycinnamic acid as matrix Sample preparation It has been ... polymer-based reversedphase column The column was maintained at 45 °C and perfused at a flow rate of mLÆmin)1 witha mobile phase containing solvent A (5 mM ammonium acetate, pH in 5% acetonitrile), and ... media and, at the same time, it has a helix-promoting ability very similar to that of trifluoroethanol [15,16] MATERIALS AND METHODS Solid phase peptide synthesis and purification Ab-(1–42) was...
... into one peak on a real mass scale, which has a typical peak width at half height of 10–20 average mass units The mass spectrometer was tuned for chromatographic conditions witha lgÆlL)1 solution ... conserved andit may reasonably be assumed that they have similar optical extinction coefficients; therefore the area underlying each HPLC peak allowed a comparison ofthe relative stoichiometry of each ... quadrupole mass spectrometer equipped withthe electrospray ion source [22] For HPLC-MS analysis, with pneumatically assisted electrospray, a spray voltage of kV anda sheath gas pressure of...