... exact same moment of time. There was the smell of the perfume she had chosen that day, the quality of her voice, the people that were in the corridor at the same time, and the way she had looked ... have the power to destroy the turtle head, but instead you play games with him?” Duana asked. It was enough of a distraction that Worf ran him through the chest with the sword, all the way to the ... Fleet’s return. The first wave of intruders arrived on the deck of the Minnesota just prior to the Captain officially hailing the planet’s leader. The Iotian’s President appeared on the view screen...
... shaded areas).Within the pocket domain (pocket subdomains A and B and the spacer region) the high-est homology between the pRb protein family is found in the A and B subdomains. The pocket-domain ... reflect the not yet fully understood specific functions of these pro-teins at these specific stages of the cell cycle.4.4pRb Family Mediated Regulation of E2F by Cellular LocalizationAnother level ... phosphorylation of these sites are involvedin the regulation of p130 specific functions and interactions. The difference inphosphorylation sites and kinases involved in the phosophorylation of thesesites...
... within the time constraints of thefamily planning clinics. On the other hand, a client is potentially done a disservice if the information provided conceals or downplays informationabout the mechanism ... Demographic information wasasked at the conclusion of the questionnaire. The Spanishand the English version of the questionnaire are availablefrom the authors upon request. The questionnaire was distributed ... offering students courses in technical and other subjects after they have left school.‡ Other: includes the other options in the questionnaire: "there is no exact time", "I am not...
... which there is no restriction for use of the method 2 A condition where the advantages of using the method generally outweigh the theoretical or proven risks 3 A condition where the theoretical ... based on a ‘welfare of the child’ assessment, as in any other couple57. The treating HIV physician should be asked to sign the Welfare of the Child form in preference to the GP as he/she is ... Page 6 of 62 There is limited and controversial data on the effect of HAART on the natural history of disease and so management of women should be the same whether receiving therapy or not....
... indicating the direct suppression of Per1, 2 and3 by these miRNAs (Fig. 3B–D). Together, these dataindicate that the miR-192 ⁄ 194 cluster is a potent anddirect regulator of the Per gene family. Endogenously ... in the absence of afunctional SCN [27]. Therefore, it would be interestingto determine the exact role of miR-192 ⁄ 194 in thesetissues. Together, the identification of inhibitorymiRNAs for the ... in ananti-phase fashion to that of the CCGs. When the other negative regulators of BMAL1, the Per and Cryproteins, are at their peak levels in the nucleus, theyfunction in complexes to suppress...
... and, in the case of equal lengt hs, a lphabe tically. The division is based on the evolutionary tree s(Fig. 4). For the amylopullulanase from Thermococcus hydrothermalis (Q9Y8I8_THEHY), the numbering ... by the fact that the reactivation of the enzymes could be achieved by the addition of an externalnucleophile to the reaction medium. Gratifyingly, inT. hydrothermalis amylopullulanase, the ... data).Therefore, in attempt to provide the first elementstowards the understanding of the functionality of the potentially valuable, heat stable GH-57 enzymes, especiallythat of the T. hydrothermalis...
... respect to the studied enzymes of the POP family, POP seems to be the most conserved enzyme. Ten conserved amino acids werefound at the active site of the enzyme of each of the studiedPOP family ... were used as the outgroup inthis analysis, as this enzyme is a close neighbor to the POP family and a member of the serine protease family S28. The NJPLOTprogram was used to display the constructedphylogenetic ... proposed that the b-propellerof P. furiosus POP does not perform the same function as the mammalian enzyme, i.e. the exclusion of large peptidesfrom the active site [25]. Clearly the role of the b-propellerhas...
... Thus, the proteins of the CYP11Bsubfamily catalysing the last biosynthetic steps are the keyenzymes for the synthesis of both mineralocorticoids andglucocorticoids.From molecular cloning of the ... evolutionand function of the hormonal system. We first cloned the genes of the CYP11B family of the guinea pig. The 11b-hydroxylase of the guinea pig showed higher substratespecificity than the aldosterone ... sequences of the CYP11B family. Theseanalyses showed that CYP11B2 proteins are more closelyrelated to each other than CYP11B1 proteins across species.Furthermore, the 3¢ UTR of the guinea pig...
... sheet with the b12 of the other monomer, and towards the C-terminus, the subsequent residues, Phe182–Lys186, pass through the a2 vicinity of the other monomer. For the FeR dimer, the N-terminal ... oxygen for the oxygenasereaction [8]. In this family, neither component of the enzyme binds flavin tightly as a cofactor, but ratherutilizes it as another substrate [8].Considering the amino ... enzymes; these reactions dependon the redox ability of the flavin species. Although the basic redox reactions are identical or similar, it is ofinterest to understand the molecular bases for the...
... enzymes. The most significant difference between the amphi-oxus and the other retinal reductases was, nevertheless,their preference for the NADH cofactor. To ourknowledge, these are the first ... Reactions were started by the addition of cofactor and carried out for 15 min at 37 °Cin0.5 mL. The amount of protein used in the reaction mixturewas 15 lg. The reaction was terminated by the ... charged amino acids at the N-terminus of the signaling sequence, but rathercontained the L24ERGR motif at the C-terminusof the hydrophobic sequence, thereby resembling the R19ERQV, R19ERKV,...
... however, there is no report showing the pres-ence of PQRFamide peptides in other vertebrates. On the other hand, we recently identified a new member of the RFamide peptide family that has either ... neuropeptide action. Wetherefore characterized the regions in the brain show-ing the expression of lamprey PQRFamide peptidemRNA using in situ hybridization. The expression waslocalized mainly in the ... werethen compared with those of other RFamide peptides.Consistent with the result of the phylogenetic analysis,lamprey PQRFamide peptides displayed the highestsequence similarity to the group...
... major bands in e and f, while the numbers at the left are the pI values of standard proteins. The arrow at the right represents the pI value of 6.84 calculated for the unmodifiedCLAP p rotein based ... the proteins reached theirequilibrium positions, the gels containing the CLAP e, f, pI standards,and buffer only were removed from their glass tubes, soaked in distilledwater for 5–10 min then ... mem-ber o f the fas ciclin family of proteins, w e w ere i nterested t oknow whether the protein – like many o ther fasci clins – hasbeen modified C-terminally with a GPI moiety [36]. The presence...
... separates the amylase group from the CGTase group, while the thin dotted line indicates the change of the border between the two parts in the C domain treeand the SBD tree.Ó FEBS 2003 a-Amylase family ... the five-domain members of the GH 13, canbe taken as an approximation of the first three domains in the four-domain members. It also shows the typicalTable 1. The enzymes from the a-amylase family ... performing the searches in the molecular biology databases (using the default parameters)to retrieve for comparison all the relevant enzymes from the a-amylase family having a C-terminal SBD. As query, the...
... neuron-specific [8]. The APP family of proteins isbelieved to play important roles in both the peripheraland central nervous systems [6]. In the former, theyare involved in the formation and ... is the b-site APP-cleaving enzyme 1 (BACE1). Here, we investigated the effectsof genetic manipulation of BACE1 on the processing of the APP family ofproteins. BACE1 expression regulated the ... relevanceof these complexes has yet to be demonstrated.As we found that BACE1 activity regulates the maturation and the processing of the three APP family members, we were interested determining whether...
... Mn-LOX-Gga gene, Northern and Southernblot analyses. (A) The Mn-LOX-Gga gene. The open box indicates the protein coding region. The solid lines show the 5¢-and3¢-UTR and the intron. An arrow ... has identified the Fe(II) ligands. These are one watermolecule and five amino acids [12]. The iron ligands are the carboxyl oxygen of the C-terminal isoleucine (orvaline) residue, the nitrogen ... (5¢-UTR) (up to the vectorsequence) and 0.6-kb of the 3¢-UTR. The GC contentaveraged 60.5%. The 5¢-UTR did not contain TATA orCAAT-like boxes. The transcription start point for the Mn-LOX-Gga...