... recombinant allergen after affinity purification using the mAb IG12 [18]. The natural allergen Phl p 1 was also foundto be capable of degrading a synthetic substrate at a papain-cleavage site after ... degradation of the full-length allergen and accumulation ofa truncated 15-kDa fragment at about pH 4.5 (Fig. 3A) . This sharpband lacked the N-terminal peptide, as the N-glycosylatedAsn residue ... are one of the earliest lineages of eukaryotic cells, andthe Giardia proteaseis the earliest known branch ofthe cathepsin B family [23].Its phylogeny confirms that the cathepsin B lineage evolvedin...
... reveals interac-tion between ERp57 andthe tip ofthe calreticulinP-domain. Proc Natl Acad Sci USA 99, 1954–1959.53 Satoh M, Shimada A, Keino H, Kashiwai A, Nagai N,Saga S & Hosokawa M ... oxidoreductase activity. The b and b¢domains, although structurally similar to thioredoxin,do not contain catalytically active cysteines. Instead, the b and b¢ domains appear to act as spacers, and areoften ... significant differences in orientation of their aanda domains. The catalytic cysteines (orange) ofthe 4 °C yeast PDIstructure face each other. (B) The b and b¢ domains (gray) of human PDI are...
... residue of Ubi-L andthe lysine of Bcl-G. The 33.5 kDa Ubi-L adduct wasdigested by V8 protease and subjected to MALDI-MS analysis. The data in the first column are the mass values obtained experimentally,whereas ... 2 and 4contain an aliquot from the hydroxylapatite purification step; lanes 1 and 2, silver-stained; lanes 3 and 4, immunostained with anti-(Ubi-L).Mobilities ofthe 33.5 kDa Ubi-L adduct and ... third column are those calculated from the V8 protease fragmentation ofthe gene products of Bcl-G and Ubi-L. The fourth column indicates the number ofthe first and last amino acid ofthe identified...
... pKT25kan .The DNA fragments encoding tentative middle domain and C-terminal one of human GRP94 were amplified by PCRandtheninsertedinanXbaI/BamHI site of bothpUT18Camp and pKT25kan. The DNA fragment ... Oral and Maxillofacial Surgery and 2Division of Oral Molecular Biology, Department of Developmental and Reconstructive Medicine, Course of Medical and Dental Sciences, Nagasaki University GraduateSchool ... binding activity. The binding activity of the C-terminal domain (100%) or its mutated forms toward the middle-C-terminal domains was quantified as the b-galactosidase activity of the bacterial two-hybrid...
... novelC-terminal actin-binding domainThirumaran Thanabalu1,2, Rajamuthiah Rajmohan2, Lei Meng2, Gang Ren4,5, Parimala R. Vajjhala4 and Alan L. Munn1,3,4,6*1 Institute of Molecular and Cell ... and growth atelevated temperature, on the one hand, and in corticalactin-patch polarization, on the other hand, are at leastpartially distinct [23]. However, there may still be a functional ... in Table 2. The rabbit polyclonal GFP-spe-cific antiserum was a gift from J. Kahana and P. Silver(Dana Farber Cancer Center, Boston, MA). The anti-actinmAb was MAB1501 from Chemicon International...
... ofthe Gq class Ga subunits, namely Gaq and Ga16have a fast rate of degradation. The results impli-cate a prominent role for the proteasome pathway indown regulation and basal turnover of ... representative autoradio-graphies of whole SDS ⁄ PAGE gels loadedwith the 35S immunoprecipitates and the arrowheads indicate the position of Gaq and Ga16. The molecular mass standards areindicated. ... Johansson et al. Proteasome degradation of GqproteinsProteasome involvement in the degradation ofthe Gq family of Ga subunitsBente B. Johansson, Laura Minsaas and Anna M. AragayDepartment...
... 5¢-ATGAGCTCTGCTTTCCTTCCGGGGA-3¢S1 5¢-ATTGAGCTCACCAGGAGGGCAGGAGG-3¢mEts-R* 5¢-GACACCTGTCGGTAACCCTTAAAGCC-3¢mGATA* 5¢-CGGAGTCGCCTAAGGAGAGATGGAGA-3¢mE-box* 5¢-AGGGTTCCCGATCGGTGTCTGAGAGA-3¢mEts-F* ... 5¢-GTGGTACCAGTAGCAGCCGCCGCAAG-3¢K3 5¢-ATGGTACCGGGCTTTAAGGGTTCCCG-3¢K2 5¢-ATGGTACCGGAAGGAAAGCAGAGCCC-3¢K1 5¢-ATGGTACCTGGTGATCCAGGGCTTGC-3¢Sac 5¢-CCGTTCCGAGCTCCGAGCAC-3¢S3 5¢-ATGAGCTCGGGAACCCTTAAAGCCCG-3¢S2 ... Ltd.(Toyama, Japan). The library was prepared bythe cleavage of the cap structures of mRNA with Tobacco acidpyrophosphatase followed by ligation with a syntheticoligoribonucleotide (5¢-GUUGCGUUACAAGGUACGCCACAGCGUAUGAUGCGUAA-3¢)...
... Theseinvestigations were supported bythe Natural Sciences and EngineeringResearch Council of Canada, the Alberta Heritage Foundation forMedical Research, CanBiocin Ltd (Edmonton, AB), andthe CanadaResearch ... structural basis of the inhibition ofthe antimicrobial activity of CbnB2 by the leader and to assist future analysis ofthe interaction of preCbnB2 with its ABC transporter protease, it isessential ... N-terminus and C-terminus are indicated, as are the residues at the start and finish of each a- helix. The position of the double glycine motif where cleavage occurs during maturation of the bacteriocin...
... vice versa within the OPA family, the AAT family, the ST3 families and the DAACS1 family. In case ofthe AAT and DAACS1families, the percentage observed hits andthe distribu-tion are as observed ... C-terminalhalves ofthe OPA, AAT, and DAACS families andthe N- and C-ter-minal halves of ST3 (open bars), between the N-terminal halves of ST3 andthe C-terminal halves of ST3 (grey bars), and ... N- and C-terminal halves of all the sequences in ST3 and involves blast searches of each sequence in the database against the wholedatabase. Unrelated families of secondary transporters of the...
... such as dynamin and the antiviral protein, Mx. Because of similarities in the molecular architecture of GBPs and members of the dynamin family, GBPs are classified as a part of the dynamin superfamily. ... Kurosaki M, Kanazawa N,Tanabe Y, Koyama T, Takeda Y, Nakagawa M, Kaki-numa S, Sekine Y et al. (2006) Expressional screening of interferon-stimulated genes for antiviral activityagainst hepatitis ... superfamily: conserved structure and molecularmechanism. Nature 349, 117–127.3 Herrmann C, Martin GA and Wittinghofer A (1995)Quantitative analysis ofthe complex between p21ras and the Ras-binding...
... Health Team and Community Advisory Committee are established, members can start the planningprocess by conducting a situation analysis. It is important to make the analysis manageable and practical ... up-to-date data canhelp ensure that efforts focus on the health and developmental needs ofthe targetpopulation. Concerns and perceptions vary by age and gender andby demographic and socio-economic ... inadequate and limited services and programmes for males or females, and then take a leadin developing and carrying out actions that address these issues.Case StudyIn 11 African countries, approximately...
... championed the cause ofthe environment and health at a time when it was not fashionable, the challenges and responses in this area are now at the centre ofthe global health agenda. Nevertheless, ... copied in Kazakhstan.• A campaign by students in Austria raised awareness of poor public transport alternatives to the private car, and increased by 50% the number of pupils and teachers who ... RepublicDenmarkEstoniaFinlandFranceGeorgiaGermanyGreeceHungaryIcelandIrelandIsraelItalyKazakhstanKyrgyzstanLatviaLithuaniaLuxembourgMaltaMonacoMontenegroNetherlandsNorwayPolandPortugalRepublic of MoldovaRomaniaRussian FederationSan MarinoSerbiaSlovakiaSloveniaSpainSwedenSwitzerlandTajikistan The former Yugoslav Republic of MacedoniaTurkeyTurkmenistanUkraineUnited...
... further substantiated by computational interactome data that predict theirinteraction [63]. Interestingly, the observation thatYap4 and Yap6 are inducedbya variety of unrelated forms of environmental ... responses are at play [1]. Specific formsof stress such as heat shock and some formsof oxidative stress [2] demand the activation ofthe heat shock factor(HSF), a modular protein consisting ofa helix–turn–helix ... in the thioredoxin and gluta-thione systems, and other antioxidants such as catalase and superoxide dismutase, among others. Yap1 istherefore central to the adaptive response to oxidative stress, ...