simple methods for the detection of hla g variants in coding and non coding

Báo cáo khoa học: "Comparison of Diagnostic Methods for the Detection of Parasites in Fish" pdf

... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the ... recovered and placed in separate vials with PBS The content of selected separate organs (oesophagus, stomach, pyloric caeca and intestine) was scraped from the lumen and epithelial lining and inspected ... microscope Stretching of these metacercariae was done by hot formalin for two minutes Then, they were kept in Eppendorf tubes with neutral formalin (4%) Diagnosis of parasitic infections * Diagnosis based...

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Cocoa Processing Methods for the Production of High Quality Cocoa in Vietnam ppt

Danh mục: Báo cáo khoa học

... under-fermentation In all of these colour categories, beans will tend to darken in colour during drying as the majority of browning reactions will occur during this process DRYING OF COCOA Drying of cocoa ... during rain and at night Also, with the basket drying shown in illustration 20, the baskets 14 need to be moved around during the day to position them in the sun During periods of heavy rain, the ... advice and liaison with the Vietnamese research Institutes involved Success Alliance is also thanked for their assistance in providing information regarding the cocoa industry in Vietnam and in the...

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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf

Danh mục: Báo cáo khoa học

... differences in the detection limit of ISH However, digestion with 200 g/ ml proteinase K caused some tissue degradation and increased background staining Digestion with 100 g/ ml proteinase K induce ... in the hybridization assay for the detection of CHV These results suggest that ISH assay using digoxigenin-labeled probe, which was optimized in this study, may be recommended for diagnosis of ... was obtained using digoxigenin-labeled probe, 100 g/ ml proteinase K pre-treatment, and overnight hybridization The practicality of digoxigenin-labeled probe is better than those of biotin-labeled...

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báo cáo khoa học: " High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes" doc

Danh mục: Báo cáo khoa học

... tggtttcgttagtctgctct tgagcgaaagtagcggg 313 V tttgggtatgcctccgt tggaggcgcttcataatact 163 VI SBEIIa-A ttgctctaaatttatgatctggct aggtggaagattgccaag 153 VII tgctcctattgatgccgat gctacatgctcaactaaataattgg ... gggaactatctaagactccgtagcac 67°C 2100 B(IV-IX) atgtggtggatgggttatgg tccatagaataaaccatcagaccg 62°C 1970 D(II-VI) atcgcgcttcctgaacctg gggctgaagcttaagacactgac 65°C 1980 D(X-XIII) gaggcagtgggcatgtgaaagtc ctagggaactatctaagactccgtagcac ... gggtatacctcggtggattc agactagtggaggcgttt 167 V gaaggtatcgtctaattgcatatct caataaattggaaggtgtctcgtt 154 IX accatttactaaggatatttacatgcaa accagaaacaggtgaaataact 151 X XI acaatacttagaggatgcatctga ggtatttctgacttgtatgaccatt...

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Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf

Danh mục: Báo cáo khoa học

... deregulation of the cell cycle by inactivating p53 and pRb respectively [9] Integration often disrupts the integrity and expression of the E1 and E2 open reading frames, which may affect the ... [21] Further typing of high risk HPV types 16 E6 was done by type-specific forward primer (FP) 5’-GAA ACC GGT TAG TAG TAT AAA AGC AGA C-3’ and reverse primer (RP) 5’-AGC TGG GTT TCT CTA CGT GTT CT-3’ ... Method in Clinico-pathogical parameter (OSMF grading according to Pindborg and T and N category according to UICC classification) in OSCC cases Clinico-pathogical Diagnosis No (%) HPV detection...

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Creating an Inclusive Environment: A Handbook for the Inclusion of People with Disabilities in National and Community Service Programs ppt

Danh mục: Điện - Điện tử

... unboxing: using System; public class Unboxing { public static void Main() { int i = 123; // Boxing object o = i; // Unboxing phải thực tường minh tường minh int k = (int) o; 14 http://www.itgatevn.com.vn ... dụng từ khoá string tương tự cách tạo thể đối tượng nào: string chuoi; chuoi = "Learning C#"; g n giá trị cho chuỗi khởi tạo sau: string chuoi = "Learning C#"; Bạn tham khảo thêm kiểu string viết ... t.DisplayCurrentTime(); int theHour = 0; int theMinute = 0; int theSecond = 0; t.GetTime( theHour, theMinute, theSecond); System.Console.WriteLine("Current time: {0}:{1}:{2}", theHour, theMinute, theSecond);...

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Báo cáo sinh học: "Comparison of different delivery systems of DNA vaccination for the induction of protection against tuberculosis in mice and guinea pigs" pps

Danh mục: Báo cáo khoa học

... capable of sustaining the protective response in mice and guinea pigs Therefore, using biodegradable microspheres in a single dose seems to be a promising strategy for stimulating long-lasting immune ... increase veterinarian and patient acceptance of vaccination by reducing the number of injections and avoiding the use of boosters containing live vectors Using this technology, vaccinologists could ... Boehringer Ingelheim, Ingelheim, Germany) and 0.5 mg of TDM (Sigma, St Louis, USA) was emulsified with 0.3 ml of an inner aqueous phase containing mg of DNA (DNAhsp65 or DNAv) or mg of recombinant...

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Statistical methods for the detection and analyses of structural variants in the human genome

Danh mục: Cao đẳng - Đại học

... including definitions and introduction to existing technology, software and algorithms in detection of CNV/ROH These will facilitate the understanding of subsequent chapters 2.1 Terminology and ... difficulties in assay design and 45 implementation (Winchester et al., 2009) resulting in a bias against detecting CNVs in segmental duplicated regions using SNP arrays For sequencing, reads that fall in ... STATISTICAL METHODS FOR THE DETECTION AND ANALYSES OF STRUCTURAL VARIANTS IN THE HUMAN GENOME TEO SHU MEI A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS GRADUATE SCHOOL FOR INTEGRATIVE...

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Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)

Danh mục: Y học thưởng thức

... angiography findings from the study patients Angiography After the MCG test, coronary angiography was performed at the discretion of the attending physicians and following the standards of the institution ... as either having relevant stenosis or not, and if the score was below 4.0, the negative predictive value of the MCG test was 100% These impressive findings suggest a role for MCG testing in the ... stenosis from the individual centers included in the meta-analysis The boundaries of the box are Tukey’s hinges The median is identified by the line inside the box The length of the box is the interquartile...

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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens

Danh mục: Môi trường

... using YG1041, YG1042, YG3003 and/ or YG7108 strain, and 21 chemicals (8.2%) formed 8-OH-Gua in the rat hepatocytes oxidized DNA as shown in Fig These sensitive strains could detect a variety of ... using different methods (gas chromatograph-mass spectrometry, 32P postlabeling method, etc.) Therefore, it is important to develop a simple rapid in vitro method for evaluating the generation of ... herbicides, fumigants, heavy metals, inorganic chemicals and natural toxins, etc These results may suggest that the utility and limitations of both the Ames test and 8-OHGua assay in detecting chemicals...

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Comparison of Methods for the Extraction of Bioflocculants from Activated Sludge

Danh mục: Môi trường

... Several bands demonstrated the same mobility in both stains, suggesting that these bands were complexes of protein and saccharide These included the band corresponding to a molecular weight of 103,000 ... 103,000 in the steaming-extracted polymer (1), the band corresponding to 38,000 in the NaOH-extracted polymer (2), the band corresponding to 24,700 in the SDS-extracted polymer (3), and the bands ... cells aggregated through a mechanism involving linkage among these cations Sato and Ose (1980) reported that the calcium content in ash of floc-forming substances extracted from activated sludge...

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Critical evaluation of diagnostic aids for the detection of oral cancer docx

Danh mục: Sức khỏe giới tính

... clinical examination and case-ﬁnding, even in primary care In experienced hands toluidine blue staining may be useful in the evaluation of oral lesions and as an adjunct in the surveillance of ... Histological diagnosis is rarely used as a gold standard Methods vary – single rinse, double rinse, ‘painting’ Confusion over inclusion of equivocal (pale) staining as positive or negative ting To date, ... carcinomas Of the 50 clinically visible lesions, 20 stained positively with toluidine blue and of these were carcinomas One of the clinically detected carcinomas was dye-negative Therefore, out of...

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INTERNATIONAL STANDARD Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp docx

Danh mục: Nông nghiệp

... Examination for O-antigens Using one non- autoagglutinating pure colony, proceed according to 9.5.4.2, using one drop of the anti-O serum (5.3) instead of the saline solution (5.2.13) If agglutination ... licensed to and installed on the computer performing the editing In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy The ISO Central Secretariat ... minutes (approximately min) Add 0,25 ml of the reagent for detection of β -galactosidase and mix Replace the tube in the water bath set at 37 °C and leave for 24 h ± h, examining the tube at intervals...

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ag doped wo3-based powder sensor for the detection of no gas in air

Danh mục: Vật lý

... on the input frequency, giving typical Fig Histogram of NO sensitivity vs Ag/Al2O3 bed temperature An increase in temperature of the Ag/Al2O3 bed (production of NO2) results in the increase in ... re¯ecting their lack of a long-range order (non- stoichiometry region of disorder nature) However, some local regions show ordered patterns (the lack of their long-range order and their heterogeneity ... Biographies Ling Chen received a BSc degree in chemistry at Guangxi Teachers College, China in 1995 She is currently completing her postgraduate course under the supervision of Dr Tsang in the...

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nmr methods for the investigation of structure and transport

Danh mục: Đại cương

... essential for the understanding of the methods used and are only brieﬂy mentioned at the beginning However, where helpful for the understanding, the relevant equations are worked out Obtaining quantitative ... treated in Sect 2.3.5 The sum in (2.46) is a superposition of sine and cosine functions The common origin depends on the index n of the result vector, the scaling of the argument on index k of the ... t D and hence the number of spins By linearity of the Fourier transform the direct relation between the peak integral and the number of spins of the corresponding chemical group holds for the...

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Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx

Danh mục: Điện - Điện tử

... 5'-CTTGCCAACGATTACATTTCCAGRGAYGARCT-3' 5' CTGGCTAACGACTACATCTCCAGRGAYGARCT-3' 5'-GGCAGTTTCAAGGCTGTGAATTTYTTYGARCG-3' 5'-CCGTAAGAAATGGTGGTCCTGACRAAYTGNGG-3' 5'-CCGTAGGCGATGGTCGTCCTAACRAAYTGNGG-3' 5'-TACAAAATACAGCGAGTGATANATRAARCA-3' ... T CACA T .G GAGA C T GA GG.GCCCAATT AC GT C.C.GCGG .G. TATTT AG.CAGGCT A T CACA G GAGA C T GACGGAT CCTGGT GCGCGTAA.C A CTGA TCC.CAATGC.A A C .T CAC G. GT GAC .G C T GAAGGTT.ACCTGT CG.A C C.ACCT ... Probe OSMb Primer CCTCGGGCTCAGGAACAAC GTC TACTGCATGGCCCAGCTGCTGGACAA CTCAGACATGA CTGAGCCCACGAAGGCC A A C .G T Figure Primer location and specificity for the OSM QPCR assay to...

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báo cáo hóa học: " The evolution of methods for the capture of human movement leading to markerless motion capture for biomechanical applications" pptx

Danh mục: Hóa học - Dầu khí

... morphological description of the human body's anatomy plus a set of information regarding the kinematic chain and joint centers The morphological information of the human body can be a general ... nearest neighbor vs(i) among the visual hull points V, where s(i) defines the mapping from the index of a surface point pi to its rigid part index In the second step, given a set of corresponding pairs ... segments were used to define segment coordinate axes Joint angles for the lower limbs for the sagittal and frontal planes were calculated as angles between corresponding axes of neighboring segments...

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báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot

Danh mục: Hóa học - Dầu khí

... 5'-CTTGCCAACGATTACATTTCCAGRGAYGARCT-3' 5' CTGGCTAACGACTACATCTCCAGRGAYGARCT-3' 5'-GGCAGTTTCAAGGCTGTGAATTTYTTYGARCG-3' 5'-CCGTAAGAAATGGTGGTCCTGACRAAYTGNGG-3' 5'-CCGTAGGCGATGGTCGTCCTAACRAAYTGNGG-3' 5'-TACAAAATACAGCGAGTGATANATRAARCA-3' ... T CACA T .G GAGA C T GA GG.GCCCAATT AC GT C.C.GCGG .G. TATTT AG.CAGGCT A T CACA G GAGA C T GACGGAT CCTGGT GCGCGTAA.C A CTGA TCC.CAATGC.A A C .T CAC G. GT GAC .G C T GAAGGTT.ACCTGT CG.A C C.ACCT ... Probe OSMb Primer CCTCGGGCTCAGGAACAAC GTC TACTGCATGGCCCAGCTGCTGGACAA CTCAGACATGA CTGAGCCCACGAAGGCC A A C .G T Figure Primer location and specificity for the OSM QPCR assay to...

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Báo cáo hóa học: "Biocompatible micro-sized cell culture chamber for the detection of nanoparticle-induced IL8 promoter activity on a small cell population" pot

Danh mục: Hóa học - Dầu khí

... Austria 3Department of Medical Engineering and Neuroprosthetics, Fraunhofer Institute for Biomedical Engineering, 66386 St Ingbert, Germany 4Vanguard AG, 12623 Berlin, Germany Page 13 of 14 15 16 17 ... cat act ccg tat ttg ata agg aac a-3’ and the reverse primer 5’-aga att cgc ata gat ctt ccg gtg gtt tct tcc tgg ctc tt-3’, containing the restriction enzyme sequences for Xho I and Eco RI, respectively, ... http://www.nanoscalereslett.com/content/6/1/505 Hassinger (Protestant Hospital, Zweibrücken, Germany) for kindly providing the thigh bones; and Dr Erwin Gorjup (Fraunhofer IBMT, St Ingbert, Germany) for isolating the hMSCs This...

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báo cáo hóa học: " Glrt-Based Array Receivers for The Detection of a Known Signal with Unknown Parameters Corrupted by " docx

Danh mục: Hóa học - Dầu khí

... between the desired signal and the BPSK interference increases Again, the O1 detector gives the best performance while the O9 detector gives the lowest ones Again, the a priori knowledge of the noise ... performance of the optimal detectors due to the joint spatial and phase discriminations between the sources Comparison of figures 2b and 3b or 4b and 5b shows again increasing performance of the ... great interest of taking the SO noncircularity of the total noise into account in the receiver’s computation as well as the capability of the optimal receiver to perform, in this case, single antenna...

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simple methods for the detection of hla g variants in coding and non coding regions