overview of molecular biological methods for the detection of pathogens causing sexually transmitted infections

Báo cáo khoa học: "Comparison of Diagnostic Methods for the Detection of Parasites in Fish" pdf

Ngày tải lên : 06/08/2014, 19:20

... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the ... normal PCR reaction of 50 µl volume (25µl of master mix, Comparison of Diagnostic Methods for the Detection of Parasites in Fish 2µl of each primer, or µl of template and 18 or 20 µl of water) - Polymerase ... Comparison of Diagnostic Methods for the Detection of Parasites in Fish al., 1998; Anderson, 1999) Our primer sets were designed for identification of different flukes and they were useful for detection...

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Statistical methods for the detection and analyses of structural variants in the human genome

Ngày tải lên : 09/09/2015, 10:14

... where the location of the unmapped read may span the breakpoint of the CNV SR analysis has the advantage of being able to pinpoint the location of the breakpoints Assembly-based AS methods, on the ... STATISTICAL METHODS FOR THE DETECTION AND ANALYSES OF STRUCTURAL VARIANTS IN THE HUMAN GENOME TEO SHU MEI A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS GRADUATE SCHOOL FOR INTEGRATIVE ... using these technologies are unfortunately limited by the density of the probes, as well as the location of the probes For example, array platforms with more than million probes have a lower detection...

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Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)

Ngày tải lên : 03/11/2012, 10:58

... large inventory of normalized mathematical indexes of abnormality It is the pattern of these mathematical indexes of abnormality, obtained from analysis of multiple cardiac cycles of the resting ... Moreover, to assess the performance of the prediction of stenosis independent of the prevalence of stenosis, the positive and negative likelihood ratios (LR) were calculated [24] A value of p

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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens

Ngày tải lên : 05/09/2013, 08:40

... supported by the research project The research on the development of the total evaluation technique for the hazardous impacts by the chemical substances towards human and ecology (the project ... and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts in isolated rat hepatocytes treated with 6-nitrochrysene, ... different methods (gas chromatograph-mass spectrometry, 32P postlabeling method, etc.) Therefore, it is important to develop a simple rapid in vitro method for evaluating the generation of reactive...

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Comparison of Methods for the Extraction of Bioflocculants from Activated Sludge

Ngày tải lên : 05/09/2013, 09:08

... of 95 ml) The A280 to A260 ratio of the UV-absorbing peak indicated it was composed mainly of nucleic acid In the profile of the NaOH-extracted polymer (B), the elution volume (80 ml) of saccharide ... compared to the other methods The SDS-extracted polymer included a small amount of material other than protein Furthermore, the steaming- and washing-extracted polymers contained large amounts of phosphorus ... buffer at a flow rate of ml/h and ml fractions were collected The void volume of the column was determined by the elution of Blue Dextran (Pharmacia) Other analyses - 147 - Journal of Water and Environment...

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Tài liệu Báo cáo khoa học: "Methods for the Qualitative Evaluation of Lexical Association Measures" doc

Ngày tải lên : 20/02/2014, 18:20

... precision values above 50% for the rst 10% of the list, but is outperformed by the t-test afterwards Looking at the rst 40% of the data, there is a big gap between the good measures (t-test, ... evaluation of lexical association measures, mainly for the following reasons: the instability of precision values obtained from the rst few percent of the data in the SLs; the lack of signicant ... frequency) and the weak measures ( and ) In the second half of the data in the SLs, however, there is virtually no difference between , , and the other measures, with the exception of mere co-occurrence...

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Critical evaluation of diagnostic aids for the detection of oral cancer docx

Ngày tải lên : 06/03/2014, 02:21

... respectively One of the weaknesses of this study is the small sample size In addition, the majority of the lesions examined appear to be of the Class I type, rather than Class II lesions for which the technology ... appropriate to the purpose of the test? Use examiners representative of the target or primary clinical providers of the test? Show evidence that the test can distinguish cancer/precancer from other conditions ... predictive value Therefore, the lack of apparent control as well as documentation with respect to the manner in which the sample was selected limits the utility of this study Poate et al performed a...

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INTERNATIONAL STANDARD Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp docx

Ngày tải lên : 07/03/2014, 16:20

... drops of the creatine solution, three drops of the ethanolic solution of 1-naphthol and then two drops of the potassium hydroxide solution; shake after the addition of each reagent The formation of ... incubation for 24 h ± h, examine the plates (9.4.3) for the presence of typical colonies of Salmonella and atypical colonies that may be Salmonella (see Note) Mark their position on the bottom of the ... the results of the interpretation, indicate the presence or absence of Salmonella in a test portion of x g or x ml of product (see ISO 7218) See annex C for the precision data obtained from the...

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ag doped wo3-based powder sensor for the detection of no gas in air

Ngày tải lên : 19/03/2014, 16:47

... the concentration of NO and NO2 gases because of the low concentration, one of the roles of the Ag is apparent to provide surface for the conversion of NO to NO2 according to the literature [18] ... one of the roles of promoter may be to provide a surface for the catalytic conversion of NO to NO2 that are responsible for the high sensitivity associated with the promotion However, whether ... and was then diffracted onto the detector The detector collected data for all angles in the range 4±648, 2y, simultaneously Fig gives the diffraction spectra of the WO3 before and after the NO...

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nmr methods for the investigation of structure and transport

Ngày tải lên : 29/05/2014, 18:32

... scrutinized the German version of this text Prof Nirschl suggested the idea of this book Prof Reimert organized the continuation of the research unit after the DFG funding as well as Prof Kasper, Prof ... derivation due to the time-dependence of the unit vectors Using the product rule for the derivation and the fact that the time derivative of the unit vector is the cross product of !rf with this ... the scaling of the argument on index k of the measured data in reciprocal space As the sum is ﬁnite, it only represents an approximation of the delta function The position of the origin and the...

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Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx

Ngày tải lên : 18/06/2014, 22:20

... module for 45 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 30 s The copy number for each assay was calculated from the cycle threshold (CT) determined using the Bio-Rad software The viral ... errors of the viral and OSM copy number determinations, as described in Materials and Methods * These results, while positive for both duplicates, were outside of the linear range of the assay the ... to those in the RRV sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses...

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báo cáo hóa học: " The evolution of methods for the capture of human movement leading to markerless motion capture for biomechanical applications" pptx

Ngày tải lên : 19/06/2014, 10:20

... knowledge related to the mechanics of human movement The work at the University of California formed the basis for many of the fundamental techniques currently used for the study of human locomotion ... patterns of locomotion requires new methods that would limit the risk of producing artifact due to markers or the constraints of the testing methods For example, the constraints of the laboratory ... limiting the advancement of the study of human movement is the measurement of skeletal movement from markers or sensors placed on the skin The movement of the markers is typically used to infer the...

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báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot

Ngày tải lên : 20/06/2014, 04:20

... module for 45 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 30 s The copy number for each assay was calculated from the cycle threshold (CT) determined using the Bio-Rad software The viral ... errors of the viral and OSM copy number determinations, as described in Materials and Methods * These results, while positive for both duplicates, were outside of the linear range of the assay the ... to those in the RRV sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses...

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Báo cáo hóa học: "Biocompatible micro-sized cell culture chamber for the detection of nanoparticle-induced IL8 promoter activity on a small cell population" pot

Ngày tải lên : 21/06/2014, 00:20

... differentiated hMSCs, also in the corner areas of the microcavity (Figure 3f) The performed studies verify the biocompatibility of the Si3N4 membrane and the suitability of the microcavity for in vitro studies ... Parkinson disease, for the development of new sensitive drug screening methods and for the quantification of toxicodynamic and toxicokinetic effects Application of the MCC for the analysis of nanoparticleinduced ... exposures Therefore, the analysis and quantification of single-cell responses will provide important information on the toxicity of the tested materials The MCC-based method is therefore qualified...

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báo cáo hóa học: " Glrt-Based Array Receivers for The Detection of a Known Signal with Unknown Parameters Corrupted by " docx

Ngày tải lên : 21/06/2014, 00:20

... (12) then corresponds to the correlation of the real part, z1c(nT), of the SMF’s output, y1c(nT), with the known real symbols, an, over the known signal duration B Performance The performance of ... detectors as the phase diversity between the sources increases We still note the best performance of the completely informed detectors (C1 and O1) and the lowest performance of the less informed detectors ... statistical theory of detection, the optimal receiver for the detection of the K symbols an from both the K primary data xv(nT) and the K’ secondary data bTv(nT)’ consists in comparing to a threshold the...

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Báo cáo hóa học: " A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA" ppt

Ngày tải lên : 21/06/2014, 01:20

... cutoff value in succedent detection was determined as a positive result The ten HPV-16-positive samples were used to validate our hybridization assay on the basis of the cutoff value Detection of ... out the molecular diagnostic study CR participated in the collection of clinical samples and part of molecular diagnostic study LD conceived of the study, and participated in its design, performed ... Page of predenatured at 95°C for 10 min, then 55°C for 30 The particles coupled with streptavidin were added into the hybridization mixtures and incubated at 37°C for 10 and enriched in the bottom...

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Cocoa Processing Methods for the Production of High Quality Cocoa in Vietnam ppt

Ngày tải lên : 21/06/2014, 06:20

... on the percentage of fully brown beans in the cut test 6: The effect of spreading of beans for two hours, prior to placement in boxes, on fermentation temperatures 7: The effect of spreading of ... reveals the presence of certain defects which may cause off-flavours and indicates the degree of fermentation of the beans which has a bearing on the flavour and quality of the beans The International ... case of larger quantities of beans e.g 250kg, partitioned boxes (Illustration 13) are used In this case, turning of beans is performed by shovelling them from one side of the partition to the other...

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báo cáo hóa học:" Research Article Linear Classiﬁer with Reject Option for the Detection of Vocal Fold Paralysis and Vocal Fold Edema" doc

Ngày tải lên : 21/06/2014, 20:20

... probability of detection close to 85% could be achieved for a probability of false alarm 10% in the case of vocal fold paralysis in male utterances, while the probability of detection for vocal ... probability of detection for vocal fold paralysis in men was measured, that is approximately equal to 90% for probability of false alarm 10% The gain in the probability of detection for vocal fold ... probability of detection approximately 73% for probability of false alarm 4% [33] For the same probability of false alarm, a probability of detection, which falls between 80.95% for rectangular...

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Báo cáo hóa học: " Quinoline Group Modiﬁed Carbon Nanotubes for the Detection of Zinc Ions" ppt

Ngày tải lên : 22/06/2014, 01:20

... selectivity when they are used just for Zn(II) detection On the other hand, the ﬂuorescence enhancement of MWNTs-GNQ is only for Zn(II) As the use of organic–inorganic hybrid materials for bio-application ... intracellular environment for the detection of Zn(II) References ð1Þ Wherein, A is the value of the ﬂuorescence intensity, C is the concentration of Zn(II), and the range of C is from 0.2 lM to ... happened in the temperature range from 200 °C to 450 °C due to the degradation of the GNQ grafted to the MWNTs The content of the GNQ grafted to the MWNTs is about 12 wt%, which is similar to the calculation...

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