position specific posterior lattices
Tài liệu Báo cáo khoa học: Bacitracin is not a specific inhibitor of protein disulfide isomerase pptx
... published evidence for the in vivospecificity of bacitracin (or potential products) for PDIinhibition. Our results clearly show that bacitracinin vitro is not a specific inhibitor of PDI, but that ... 0.05Bacitracin is not specific for PDI A R. Karala and L. W. Ruddock2456 FEBS Journal 277 (2010) 2454–2462 ª 2010 The Authors Journal compilation ª 2010 FEBSBacitracin is not a specific inhibitor ... many have been shown to catalyze thiol–disulfideexchange reactions. However, their specific roles,substrate specificities and mechanisms of cooperationwith other catalysts and chaperones in the...
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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
... and 6-fold that of nNOSred(9.0 s)1). Calculated values for specificity constants(kcat⁄ Km) reveal that CPR has the greatest specificity(10.6 · 105m)1Æs)1) for AqCbl, with MSR(4.1 ... MSR FMN semiquinone(FMNsq) or FMN hydroquinone (FMNhq) to thehMS-bound cob(II)alamin [19]. Specifically, the mid-point potential values for FMNox ⁄ sqand FMNsq ⁄ hqare respectively 380 ... Reactivation of hMS was notobserved when MSR was replaced by nNOSred orCPR, highlighting the need for specific protein–proteininteractions between MSR and hMS. Reactivation ofhMS was found to be dependent...
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Tài liệu Báo cáo khoa học: The cartilage-specific transcription factor Sox9 regulates AP-2e expression in chondrocytes pptx
... immunoprecipitation(ChIP) assays were performed using SW1353 cells anda specific Sox9 antibody. DNA samples were analyzedby PCR using specific primer pairs generating frag-ments spanning the first (Sox9_1) ... differentiation of themesenchymal cells to chondrocytes causes a change inECM composition. Chondrocytes express cartilage- specific type II, type IX and type XI collagen,proteoglycans, aggrecan, and ... embryos were performed using a specific polyclonal antiserum against AP-2e [31](Fig. 2A). AP-2e was detected in hypertrophic areas ofthe cartilage. To verify the specificity, we stained sec-tions...
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Tài liệu Báo cáo khoa học: Pyrimidine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc
... fasciculata; CU-NH, pyrimidine -specific ribonucleoside hydrolases; Ec, Escherichia coli; IAG-NH, purine -specific inosine-adenosine-guanosine nucleoside hydrolases; IG-NH, 6-oxo-purine -specific inosine-guanosine ... charac-terization defines SsCU-NH as a pyrimidine -specific NH. This enzyme was completely inactive towardsadenosine and guanosine. SsCU-NH, in analogy withEc-Yeik enzyme, is specific for uridine and cytidineand ... server [62] was used to perform predictionson type and position of secondary structure elements onSsCU-NH, whereas the analysis of type and position ofsecondary structure elements on the templates...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... that Lyve-1 expres-sion in liver sinusoidal ECs, reported as a marker ofdifferentiated organ -specific ECs [32] and a potentialdiagnostic marker of liver cancer and cirrhosis [30], isregulated ... [36]. These results suggestthat growth factor signals were transduced properlyvia endothelium -specific receptors in these cells. Mes-enteric BECs and LECs (Fig. 5A,B) were also obtainedby the ... mesenchymal fibroblast cells,even though a conditional genetic modification such asendothelium -specific knockouts can provide a partialsolution to this problem. Therefore, the isolation andmaintenance...
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Tài liệu Báo cáo khoa học: Seed-based systematic discovery of specific transcription factor target genes pptx
... 200000.050.10.150.20.250 5000 10 00000.10.2 Position of gene in the rankingDensity of occurence"high rank""low rank" ;position DensityGenes involved in immune responseFig. ... then analysed regarding its position in the rank. This procedure was repeated 100times. It turned out that the test group members werestrongly present in the top positions of the rank, andthis ... 20 000 10 20 30 40 E2F ETS1 HIF-1 HNF4 NFkB Number of genes Number of genes Position in rank Position in rank 0 10 0 10 000 5000 0 10 0 10 0 10 0 10 5 5 5 5 5 Cross-validation...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... Reed M, Wang P &Tegtmeyer P (1997) Reciprocal interference between thesequence -specific core and nonspecific C-terminal DNAbinding domains of p53: implications for regulation.Mol Cell Biol ... targetsites possessing a single GG motif at various positionsmay differ significantly (more details will be publishedelsewhere).Altered sequence-nonspecific interactions of the p53protein with DNA ... the nonspecific cisPt-DNA[34]. Furthermore, we were interested in whether thepresence of cisplatin adducts within DNA stretchesflanking the p53DBSs affects the ability of p53 to bindthe specific...
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Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
... stationary growth phase, suggestingthat ICis specifically associated with the vacuolarmembranes rather than the other cellular membranes.The lipid composition of subcellular membranes in theyeast ... about the content of phosphoinosi-tides in vacuolar membranes and the variation ofphospholipid compositions at different growth phasesis available. Thus, it remains to be resolved if the tar-geting ... phosphoinositides. Therelocation of ICleads us to propose a working modelin which ICin the cytoplasm is specifically targeted toanionic phospholipid molecules in the vacuolar mem-branes during the stationary...
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Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt
... acid triadsfor serine protease. Arrowheads indicate the positions and direc-tions of the PCR primers. Primers F1a, F1b, and F1c were specific for exons 1a, 1b, and 1c, respectively. The sequences ... are described in Experimental procedures. (B) Tissue -specific expression of alternative KLK11 transcripts. RT-PCR was performedusing primers specific for each alternative transcript, F1a, F1b, orF1c, ... and are expressed in a tissue -specific manner [23].Human and mouse 5¢ alternative transcripts ofKLK6 ⁄ neurosin display identical genomic organizationand tissue -specific expression. We propose...
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Tài liệu Báo cáo khoa học: Parvalbumin deficiency in fast-twitch muscles leads to increased ‘slow-twitch type’ mitochondria, but does not affect the expression of fiber specific proteins doc
... volume and a biochemical composition as found inslow-twitch muscle. Also with respect to ATP contentin a resting muscle, which is apparently regulated bythe muscle-type specific mitochondria, TA ... by 2D high-performance thin layerchromatography (HPTLC) on silica gel HPTLC plates(Merck). The position of cardiolipin on the plates was identi-fied by chromatography of a cardiolipin standard ... c.On the other hand, the up-regulation of F1-ATPase bwas clearly smaller and comparable to the compositionof SOL mitochondria, indicative of a regulated mech-anism, as discussed below. In addition,...
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Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
... solvent during the nonspecificchitosanolysis by papain and reported lactic acid to be abetter solvent owing to the hydrolyzing action of the former[28]. Contrary to this, the specific activity of ... enzyme notablyshowed a much higher specific activity towards proteolysisof casein (4.14 U) as compared to 1.15 U towards chitosandepolymerization. Although the specificity of pronase wasmuch lower ... on chitosanolysis by pronasePreliminary studies indicated that chitosan could be degra-ded nonspecifically by proteases such as pepsin, papain,protease, etc. It was observed that chitosanolysis...
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Tài liệu Báo cáo khoa học: Specific interaction between the classical swine fever virus NS5B protein and the viral genome pdf
... 3¢-noncodingregion of the viral RNA is specific and requires the 3¢-poly(A) tail.J. Biol. Chem. 268 , 26093–26098.36. Banerjee, R. & Da sgupta, A. ( 2001) Specific interaction o f hepa-titis ... than the plus-strand 3¢ UTR.Mapping of the specific NS5B-binding sequenceon the 3¢ UTRFrom the a bove experimental r esults, it i s d erived that a specific element recognized by the viral RNA ... report shows that the CSFVRdRp originating from expression in prokaryotic cells hashigh template specificity. Replacement and deletion in themiddle part of the viral 3¢ UTR has little influence...
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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells pdf
... FACS those cells specifically labeledwith destabilized intrabody. The observed stabilizationeffect upon binding of the short-lived anti-b-gal scFvsmay be useful for improving specific imaging ... (Programme ‘Prote´omique etGe´nie des Prote´ines’) and the Association de laA P. Sibler et al. Specific antigen detection with destabilized intrabodyFEBS Journal 272 (2005) 2878–2891 ª 2005 FEBS ... binding-defective7A-d1-EGFP proteins (Fig. 5G), indicating that thebinding of the destabilized scFv allows the specific detection of low amounts of intracellular target.While performing these experiments, we...
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Tài liệu Báo cáo khoa học: A novel nuclear DNA helicase with high specific activity from Pisum sativum catalytically translocates in the 3¢fi5¢ direction docx
... sub-strates with tail(s), no tails and small linear syntheticsubstrates as well as direction -specific substrates (seeFig. 5A–J). The sequences and details of these oligo-nucleotides have been ... used in this study are shown inFig. 5A–J. All the M13 substrates (Fig. 5A–F) includingdirection specific substrates (Fig. 5I and J) and smallsynthetic oligonucleotide substrates (Fig. 5G and H) ... kit.1736 T N. Phan et al. (Eur. J. Biochem. 270) Ó FEBS 2003A novel nuclear DNA helicase with high specific activity fromPisum sativumcatalytically translocates in the 3¢fi5¢ directionTuan-Nghia...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... are sugar -specific proteins with multiple combiningsites capable of agglutinating cells or precipitating glyco-conjugates [1]. Lectins may recognize specifically the wholesugar [2], a specific ... The lectin from Scylla serratawas NeuGc specific [24] and that from Cancer antennariuswas 9-O-NeuAc and 4-O-NeuAc specific [12,28]. Lectinswith defined specificity for different kinds of sialic ... O-acetyl NeuAc specificity of P. jacquemontii lectinis sufficiently evident from its inhibition and hemagglutin-atin study. The lectin is unique from that of other sialic acid- specific lectins....
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