... 1) were supplied by VBC Genomics (Vienna, Austria) Plasmids pPGM1 and pPGM4 [derivatives of pBSK(+) vector containing the PGM1 and PGM4 sites; Fig 1] were prepared as previously described [17,33] ... 34 Pivonkova H, Brazdova M, Kasparkova J, Brabec V & Fojta M (2006) Recognition of cisplatin-damaged DNA by p5 3 protein: critical role of the p5 3 C- terminal domain Biochem Biophys Res Commun ... Bartek J, Midgley CA & Lane DP (1992) An immunochemical analysis of the human nuclear phosphoprotein p5 3 New monoclonal antibodies and epitope mapping using recombinant p5 3 J Immunol Methods 151,...
... induce mitochondrial fragmentation and programmed cell death (PCD) [136] The overexpression of DRP-1 can induce PCD, indicating an evolutionary conservation of mitochondrial involvement in PCD ... trafficking to the vacuole (vacuolar protein sorting) [105–107] Vps1 also participates in clathrin-dependent trafficking from the Golgi via a prevacuolar compartment to the plasma membrane This pathway ... of DRPs are Mx proteins, mammalian DLP1, and the yeast proteins Dnm1, Mgm1 and Vps1 Mx proteins are interferon-inducible DRPs [98] Like dynamin, they self-assemble and bind and tubulate lipids,...
... (reverse) For p1 5 the primers used were CAGAGCTGTTGCTCCTCCAC (forward) and CGTGCAGATACCTCGCAATA (reverse) For p2 1 the primers used were AGCAAAGTATGCCGTCGTCT (forward) and ACACGCTCCCAGACGTAGTT (reverse) ... PCR L of cDNA template was amplified in a 25-L reaction volume of GeneAmp PCR buffer (Applied Biosystems), containing 5.5 mM MgCl2, 200 M of each dNTP, 0.5 M of appropriate primer pairs and ... Cip/Kip family (p2 1, p2 7, and p5 7) show broader substrate specificity inactivating both cyclin D-CDK4/6 and cyclin ECDK2 kinase complexes [28] We examined the expression of p1 5INK4, p2 1WAF1/Cip1...
... Gag and Pol molecules per particle Prokaryotic expression and purification of viral proteins The cloning strategy [9,10] and the purified recombinant proteins are depicted in Fig pETgag2 was made ... Lindemann D, Rethwilm A: Improved primate foamy virus vectors and packaging constructs J Virol 2002, 76:3774-3783 Moebes A, Enssle J, Bieniasz PD, Heinkelein M, Lindemann D, Bock M, McClure MO, ... determination of the relative amounts of Gag and Pol proteinsin purified PFV Representative example of the determination of the relative amounts of Gag and Pol proteinsin purified PFV (A) Extracellular...
... implicates a major chemotherapeutic advantage considering that tumor cells often overexpress antiapoptotic Bcl-2 and introducing pro-apoptotic Bcl-2 family mimetics can specifically target and ... discovered and documented These Bcl-2 family proteins were generally classified into two major groups, namely pro-apoptotic and anti-apoptotic Some of the proapoptotic proteins include Bax and ... survival-promoting pro-oxidant state by Bcl-2 in tumor cells, from the perspective of an involvement in mitochondrial functions and COX activity Interestingly, during our study, a novel, unexpected...
... primer or lM degenerate primer, lM lambda arm primer (PF or PR), 200 lM each dNTP, 1.5 mM MgCl2, and 50 mM KCl in 20 mM Tris/HCl (pH 8.4) PF (forward primer) and PR (reverse primer) are complementary ... and primer sets P2 -1 and PR Gene-speci c PCR primers for Psrp-2, Psrp-3 and Psrp-4 (P2 -2, P3 -2 and P4 2) were designed from the nucleotide sequence of PCR products, P2 -1/PR, P3 -1/PR and P4 -1/PR ... higher plant chloro-ribosome (Table 1) In terms of protein character PSRPs can be divided into two groups: (a) acidic proteins, PSRP-1, PSRP-2 and PSRP-3; (b) small/basic proteins, PSRP-4, PSRP-5 and...
... each volume As a rule, no reprints of individual contributions can be supplied Manuscript Submission The manuscript in its final and approved version must be submitted in ready to print form The corresponding ... or Springer-Verlag LNP Online archive: springerlink.com Vol.588: Y Watanabe, S Heun, G Salviati, N Yamamoto (Eds.), Nanoscale Spectroscopy and Its Applications to Semiconductor Research Vol.589: ... Turbulence and Magnetic Fields in Astrophysics Vol.615: J B¨ chner, C. T Dum, M Scholer (Eds.), Space u Plasma Simulation Vol.616: J Trampetic, J Wess (Eds.), Particle Physics in the New Millenium Vol.617:...
... growthHPIV1 wt and rHPIV1 mutant viruses containing the indicated mutations in the P/ C, HN and Comparison Comparison of the replication of HPIV1 wt and rHPIV1 mutant viruses containing the indicated ... parainfluenza virus type vaccine candidate by replacing the HN and F glycoproteins of the live-attenuated PIV3 cp45 vaccine virus with their PIV1 counterparts Vaccine 1999, 18:503-510 Clements ML, Belshe ... Murphy BR, Prince GA, Collins PL, Van Wyke Coelingh K, Olmsted RA, Spriggs MK, Parrott RH, Kim HW, Brandt CD, Chanock RM: Current approaches to the development of vaccines effective against parainfluenza...
... growthHPIV1 wt and rHPIV1 mutant viruses containing the indicated mutations in the P/ C, HN and Comparison Comparison of the replication of HPIV1 wt and rHPIV1 mutant viruses containing the indicated ... parainfluenza virus type vaccine candidate by replacing the HN and F glycoproteins of the live-attenuated PIV3 cp45 vaccine virus with their PIV1 counterparts Vaccine 1999, 18:503-510 Clements ML, Belshe ... Murphy BR, Prince GA, Collins PL, Van Wyke Coelingh K, Olmsted RA, Spriggs MK, Parrott RH, Kim HW, Brandt CD, Chanock RM: Current approaches to the development of vaccines effective against parainfluenza...
... Expression of recombinant HCV proteins Expression of recombinant HCV proteins A Individual HCV genes were cloned into pcDNA3.1(+) plasmid under CMV promoter The expression of HCV proteins was verified ... recombinant HCV proteinsin insect cells To produce recombinant HCV proteins individual HCV genes from genotype 1b cDNA were amplified with PCR and the products were subcloned into the pcDNA3.1(+)FLAG ... recombinant HCV proteins Detection of anti-HCV antibodies in HCV RNA and antibody positive human sera with recombinant HCV proteins μg of each baculovirus-expressed and preparative SDS-PAGE-purified...