... recruitment of MOF to recently transcribed regions, especially at the3¢ ends of genes where H3-K36me3 is enriched [128].In mammalian cells MOF also exists as partof a sepa-rate MOF–MSLv1 complex ... withbinding of both MOF and Tip60 (equivalent to Esa1in yeast NuA4) [124,130]. However, in human cellsdepletion of MOF, but not Tip60, results in reducedglobal levels of AcH4-K16 and defective ... acetylation and deacetyla-tion, and transient recruitment of remodellers and transcription factors.A cycle of transcription commences with the recruit-ment of transcription factors and co-factors...
... of dendritic arborizationpatternsTo quantify dendrite growth and branching, 15 neuronstransfected with each construct were randomly chosen for each experiment, and EGFP fluorescent images of ... MAP2 cDNA and its derivativesgenerated by PCR were cloned into pGEX-4T-2. The KINDdomains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2. The N- and C-terminal ... stability and rear-rangement of microtubules in neuronal dendrites[13–15] and is associated with some neurological and psychiatric disorders [16,17]. However, the structure– function relationship of...
... predicting,measuring and understanding the roles of different kinds of organisms in the flux and storage of elements in ecosystems. The total biomass per unit area, W,issimply the sum ofthe body mass of all ... NC 28557 and Virginia Institute of Marine Science, The College of William and Mary, Gloucester Point, VA 23062.Michael C. Marshall Department of Zoology and Physiology, University of Wyoming, ... Zealand.Lusha M. Tronstad Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA.Philip Warren Department of Animal and Plant Sciences, University of Sheffield,Western...
... superposition of the exponential rise to analyzequantitatively the contribution ofeach phase. Theamplitude and rate constant ofeachof the threephases can be calculated by deconvolution of thetraces ... use of FI in the study of PSII electron transport and to obtain a more complete analysis of the O–J,J–I and I–P rises. This method was also used to findthe apparent activation energy ofeach ... type of nonlinear regressionshows that the method can be used as an excellentapproximation of FI traces and to quantitatively esti-mate the contribution ofeach phase. The average val-ues of...
... extent of membranelocalization of Noxo1b and Noxo1c. Theintensities of immunoreactive bands forHA-Noxo1b and HA-Noxo1c in (B) werequantified using a LAS-1000plus (Fuji film)image analyzer and ... activity of the PXdomains of Noxo1b and Noxo1cThe membrane localization of Noxo1b is mediated in part by binding of the PX domain to membrane phos-pholipids [19]. Less association of Noxo1c ... SH3 domain of Noxo1 participates inregulation of Nox3, the role of the PX domain in Nox3activity remains unknown.In the process of cloning of human Noxo1, somespliced transcripts of the NOXO1...
... prosegment and total or partial removal of the internalFig. 1. Plant aspartic proteinase precursors. Comparison of the aminoacid sequences of representative members of the A1 family of plantaspartic ... The structure andfunctionof the asparticproteinases. Annu. Rev. Biophys. Biophys. Chem. 19, 189–215.2. Dunn, B.M. (2002) Structure and mechanism of the pepsin-likefamily of aspartic peptidases. ... by the prosegment, but also bythe 13 residues of the N-terminal of the mature enzyme and by the ÔflapÕ. The anchorage of the prosegment andofpart of the N-terminus in the active site cleft is...
... domain ofeach subunit contri-butes to the formation of the hydrophilic pore. ACh binding proteinhas structural and functional homology to the extracellular ligandbinding domain of the nAChR, and ... based onsingle channel recordings and the use of a-conotoxins MII and AuIB further dissected and correlated the combinato-rial and functional heterogeneity of the slowly decayingpopulation ... the identification and characterization of a-conotoxins with selectivity for neur-onal nAChR subtypes and provides examples of their use indefining the compositions andfunctionof neuronal nAChRsubtypes...
... de note the five residues of on e of the threesides of s bwAFP o r s ix residues of on e of two sides of T mAFP. T hestrands that make up t he three, parallel b-sheets of t he pro tein aredesignated ... addition of anadditional one, two or three b-helical c oils compared to theshorter isoforms. In the case of one sbwAFP isoform,named CfAFP-501, a detailed e xamination of t he structure and function ... D. SykesCIHR Group in Protein Structure and Function, Department of Biochemistry and Protein Engineering Network of Centres of Excellence, University of Alberta, Edmonton, Alberta, CanadaAntifreeze...
... activation andfunctionof the cytokine Spa¨tzlein the innate immune response of a lepidopteran insect,Manduca sextaChunju An1, Haobo Jiang2 and Michael R. Kanost11 Department of Biochemistry, ... byrecognition of microbial patterns and by microbialproteinases. Further biochemical and genetic researchis required for a more complete understanding of theextracellular reactions of plasma proteins ... collected, and fat body RNA samples were prepared from each insect.(A) Antimicrobial activity of plasma assayed against E. coli, and identification of antimicrobial plasma proteins by SDS ⁄ PAGE and...
... minidomain and the zinc-binding domain of the zinc finger protein Ynr046w [25] (Fig. 8). The fit of the heavy atoms (Ca, C, and N) from threeb-strands and the a-helices of both the closed and theopen ... variation of pH in the rangebetween 6.3 and 7.7, of ionic strength between 25 and 100 mm NaCl andof temperature between 278 and 313 K did not lead to any detectable change in thepopulations of the ... that of the corresponding part of the crystal structure [19]. Four b-strands (b1, 301–303;b2, 320–323; b6, 389–392; and b7, 409–412) form ab-sheet with three antiparallel strands (1, 2 and 7) and strand...
... dublin TLP and zebrafish TLP are found in the flex-ible portions of strands B and C that protrude towardsthe solvent and in the conformation of the long loopconnecting strands D and E [17]. ... present and discuss recent find-ings regarding the identification and distribution of TLP genes in nature, the structural and functionalcharacterization of the TLP from various organisms, and the ... Interruptions in the b-strands A, G and H are also observed in TLP structures as a result of alterations to the formation of hydrogen bondsbetween strands. The carbonyls of residues V104 and P105 (zebrafish...
... ARTICLESynthesis andfunctionof ribosomal proteins – fadingmodels and new perspectivesSara Caldarola, Maria Chiara De Stefano, Francesco Amaldi and Fabrizio LoreniDepartment of Biology, University ... maturation and export of the two ribosomal subunits [1]. Most of the constitu-ents of the preribosomal particles have been identifiedin yeast by exploiting the potent combination of genetic and biochemical ... steps of ribosome biogenesis and ⁄ or ribosome function. Indeed, a systematic study of the incorporation of RPs into preribosomes led tothe identification of the in vivo assembly pathway of the...
... which the b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adja-cent and antiparallel to the b1-strand (Fig. 1). Thelength and sequence of the variable ... syndrome and paraneoplastic disease, are associated with the loss offunctionof a particularKH domain. Here we discuss the progress made towards understanding bothgeneral and specific features of ... b-sheetcomposed of three antiparallel b-strands is abutted bythree a-helices (a1, a2, and a¢). The b-sheet in type IKH domains consists of three b-strands in the orderb1, b¢ and b2. The b1-strand and...
... part that holds two of the Fe-S clusters in the small subunit of the D. gigas enzyme is missing in HoxY of the SH, suggesting anexposure ofpartof the hydrophobic surface of the HoxH subunit. ... resulted in the total loss of thisactivity when assayed in the usual way, i.e. after addition of H2, a catalytic amount of NADH and the subsequentaddition of BV. Restoration of this activity (to ... effect of FMN, but not of FAD or riboflavin, has been noticedearlier [36,37].Figure 4 shows the effect of the FMN concentrationon the reconstitution of the activity of the reduced SH.Addition of...
... labeledwith a star.Ó FEBS 2002 Structure andfunctionof GlcNAc kinase (Eur. J. Biochem. 269) 4215Structure andfunction of N-acetylglucosamine kinaseIdentification of two active site cysteinesMarkus ... characterization of GlcNAc kinase and cysteine mutantsIn order to get a more detailed insight into the functions of specific cysteine residues of GlcNAc kinase, the Kmvaluesfor both substrates, GlcNAc and ... usingtheRASMOLsoftware.RESULTSConstruction of cysteine mutants and functionalexpression inE. coliBL21In an earlier study, the use of specific thiol-modifyingchemical reagents revealed the presence of...