... shared secondary structure characteristics of transthyretins and TLPs are indi-cated above the alignment: motifs A –C’ are indicated with straight lines and are labelled; b-strands are indicated ... 0Loktanella vestfoldensis SKA53 Proteobacteria, Alphaproteobacteria 2 0Roseovarius sp. HTCC2601 Proteobacteria, Alphaproteobacteria 2 0Ralstonia eutropha H16 Proteobacteria, Betaproteobacteria ... colonizevarious animals. Uric acid is secreted on the surface of mucosal epithelial tissues of all animals as part of theinnate immune system [47] and is also thought to actas a microbicidal agent...
... to pain and was effective inalleviating chronic pain and accelerating functional recoveryin an animal model of neuropathy. These data are inagreement with an important role of nAChRs in painperception, ... interfaces within neuronal nAChR subunitcombinations (compare Fig. 2A C)4.Sofar ,a- conotoxinsselectively targeting mammalian a3 b2 (a- MII, a- GIC) a6 b2 (a- MII, a- PIA), a3 b4 (a- AuIB) and a7 (a- ImI) ... investigation and understanding of their structure- activity relationships,may start to provide a rational way to develop additionalpharmacological tools for the elucidation of nAChR structure and function. Ó...
... Zeta, Phi, Tau and O mega [3,4,9]. Whereas Zeta, Theta and Omega classes of GSTs are found in plants and animals, the large Phi and Tau classes a re unique to plants [9]. In maize ( Zea m ays L),42 ... fractional residual activity of thepartial active enzyme intermediate, and kfast and kslowarethe rate constants for the slow a nd fast phase of the reaction.Analysis was performed using theGRAFIT(ErithacusSoftware ... probing the structureandfunctionof glutathione transferasesGeorgia A. Kotzia and Nikolaos E. LabrouLaboratory of Enzyme Technology, Department of Agricultural Biotechnology, Agricultural University...
... complexthan in T cells, with several peaks of nuclease hyper-sensitivity [85]. Mast cells express GATA-2 as well asNFAT and AP-1, and GATA-2 initiates the formation of an additional discrete GATA-2 ... typically recruitHATs such as SAGA and NuA3, which mainly acety-late histone H3, and NuA4 which acetylates histone H4on K5, K8 and K12. This cascade of events leads torecruitment of transcription ... H4-K16,the NuA4 group of HATs are essential for H4-K5, K8 and K12 acetylation [136–138]. In yeast, this group ismade up of NuA4 and Piccolo NuA4 which both uti-lize Esa1 as the HAT, and Esa1 was found...
... Science Institute, Saitama, Japan2 JST, CREST, Kawaguchi, Saitama, Japan3 Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo, Japan4 Faculty of Science and Technology, ... co-immunoprecipitation.Primary cultures, transfection and imaging of hippocampal and cerebellar neuronsHippocampal and cerebellar dissociated primary cultureswere prepared from ICR mice (Nippon SLC, Hamamatsu,J. ... circuits, and also may provide a clue to theunderstanding of some MAP2-associated neurodegen-erative and psychiatric disorders [16,17].Materials and methodsAnimalsMice (ICR) were purchased from...
... spp.1Stylatula elongata1Xenia spp.1Actinaria (sea anemones) Anemonia viridis1Anthopleura elegantissimaCorynactis californica1Metridium senileZoanthidea Palythoa variabilisScleractinia (hard ... Lepas pectinataCopepoda Centropages typicusStylasterina (Hydrozoan hard corals) Stylaster californicus1Octocorallia (soft corals & sea pens) Paracyanthus stearnsi1Sansibia spp.1Stylatula ... Data on rmaxfor a wide variety of organisms, from unicellular eukaryotes to invertebrates and vertebrates, have been compiled and analyzed by Savage et al.(2004b). Thesedata give a slope of...
... relationship and tertiary structure. Plant APs have been distributed among families A1 , A3 , A1 1 and A1 2 of clan AA, and family A2 2 of clanAD. The majority of plant APs belongs to the A1 family,together ... Molecular and biochemical characterisation of two asparticproteinases TcAP1 and TcAP2 from Theobroma cacao seeds.Planta 215, 754–762.12. Park, H., Yamanaka, N., Mikkonen, A. , Kusakabe, I. &Kobayashi, ... phytepsin was purified from barley (H. vulgare)(acces-sion number: X56136); AtAsp1, AtAsp2 and AtAsp3 are A. thalianaaspartic proteinases (accession numbers: U51036, AY070453 and AF076243, respectively);...
... the structureand dynamics of each protein, and present a comparison of s bwAFP and TmAFPwitheachotherandwithproteinsthathaveasimilar fold. Structure of sbwAFP and TmAFPThe structureof sbwAFP ... growth.ConclusionAnalysis of the structureand examination of the i ce-bindingbehaviour and point mutants of s bwAFP and TmAFPprovides an explanation for their hyperactivity compared tothe previously characterized ... the case of one sbwAFP isoform,named CfAFP-501, a detailed e xamination of t he structure andfunction was undertaken [57]. An overall match of 66%amino-acid identity was observed, with an insert...
... 389–392; and b7, 409–412) form a b-sheet with three antiparallel strands (1, 2 and 7) and strand 6, which is parallel to strand 2. b-Strands arelocated between the four a- helices (a1 , 278–294; a2 ,305–313; ... signal of the preceding amino acid, correlating the amide HN and the Ca signals, correlating the amide HN and the Ca signal of the preceding amino acid, correlating the amide NH withthe Ca and ... protein backbone, and formore than 78% of the side chain atoms.The main set of backbone u and w dihedral angles wascalculated from the chemical shift values of backboneatoms13Ca,13Cb,13C¢,1Ha,1HN,...
... The Authors Journal compilation ª 2010 FEBSStaphylococcus aureus elongation factor G – structure and analysis ofa target for fusidic acidYang Chen, Ravi Kiran Koripella, Suparna Sanyal and Maria ... Helgstrand M, Mandava CS, Mulder FA, Liljas A, Sanyal S & Akke M (2007) The ribosomal stalk bindsto translation factors IF2, EF-Tu, EF-G and RF3 via a conserved region of the L12 C-terminal ... 4714177E-mail: maria.selmer@icm.uu.seDatabaseThe atomic coordinates and observed structure factors are available in the ProteinData Bank database under the accessionnumber 2XEX(Received 22 April...
... b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adja-cent and antiparallel to the b1-strand (Fig. 1). Thelength and sequence of the variable loop are differentin ... RNA. The tandem KH1–KH2 domains of NusA recognizeRNA ligand 5¢-GAACUCAAUAG. (A) The KH1–KH2 domains of NusA bound to cognate RNA ligand (Protein Data Bank entry 2ASB). TheRNA–protein contact ... orderb1, b¢ and b2. The b1-strand and b2-strand are parallelto each other, and the b¢-strand is antiparallel to both(Fig. 1). This all-antiparallel arrangement of strandsdistinguishes the type...
... 5¢-GGAACAGGCTCCAACAGTAGGCTTATCAACCCTGATGGC143S 5¢-GATGGCTCCGAGAGTGGCAGTGGAGGCTGGGGC211S 5¢-CCCATTTGTATAGGGACTTTGATAAAAGTAAGTTTGCTGGATTTTGCCAGAAAATTGCC217S 5¢-GCTGGATTTAGTCAGAAAATTGCAGAAGGTGCACATCAGGGC268S ... presumably with different regulation.GlcNAc kinase has been cloned from man [8] and mouse[9]. Like N-acetylgalactosamine kinase [10] and N-acetyl-mannosamine kinase, as a part of the bifunctional ... generation of GlcNAc kinasecysteine mutants by site-directed mutagenesis. Mismatches with thetemplate are underlined.Name SequenceC45S 5¢-GGCACAGACCAGAGTGTGGAGAGGATCA ATGAGC131S 5¢-GGAACAGGCTCCAACAGTAGGCTTATCAACCCTGATGGC143S...
... cal-orimetry, of bovine b-andj-casein, recombinant human a- ,b-andc-synuclein, together with the A3 0P and A5 3T mu-tants of a- synuclein associated with familial cases of Par-kinson's disease, and ... the A3 0P and A5 3T mutants of a- synuclein that cause f amilial c ases of Parkinson's disease.Unfortunately the quality of s ome of these syn uclein ROAspectra i s generally not as good as ... temperature backscatteredRaman a nd ROA spectra of bovine b-casein (top pair) and j-casein (bottom p air) at pH 7.0. Overall, the ROA spectraare very much a like, demonstrating that the basic...
... Ile-Glu-Ala-Arg-p-nitro-anilide (IEARpNA) [31]. ProHP8Xalacked IEARaseactivity, but after the zymogen was activated by factorXa, IEARase activity increased significantly above that of factor Xa alone, which could also ... thesubstrate (Fig. 6B). These results indicated that factorXa cleaved and activated proHP8Xa.When activated HP8Xawas mixed with proSpa¨tzle- 1A, the 38 kDa pro-Spa¨tzle band disappeared, and ... (2004)Beta-1,3-glucan recognition protein-2 (betaGRP-2)fromManduca sexta; an acute-phase protein that bindsbeta-1,3-glucan and lipoteichoic acid to aggregate fungi and bacteria and stimulate prophenoloxidase...
... &Glomset, J .A. (1994) Rab geranylgeranyl transferase catalyzesthe geranylgeranylation of adjacent cysteines in the samllGTPases Rab 1A, Rab 3A, and Rab 5A. Proc. Natl. Acad. Sci. USA 91, 11963–11967.89. ... FTase incomplex with FPP [38] as well as the structureof the ternarycomplex containing an inactive FPP analogue anda CaaXpeptide substrate [39]. Many features of the apo-FTase structure are ... Synthesis and characterization of aza analog inhibitors of squalene and geranylgeranyl diphosphate synthases. J. Org. Chem. 57, 3444–3449.75. Sagami, H., Korenaga, T., Ogura, K., Steiger, A. , Pyun,...