... however,only applicable to the case when for each node there areas many parameters as the number of overall network nodes and these parameters do not directly affect the corresponding node. The fundamental ... experimental data containing (partially) cor-rect dynamic pattern changes then we can infer the (partial) interaction structureofthe underlying cellular network by integrating the analysis results ... without a speci-fic assumption on parameter perturbations for each network node. Basedon these equations, we further describethe available specific informationfrom each part of experimental data...
... a ⁄ b domain than in thea domain [20].This is also reflected in the rigidity ofthe protein. The atomic displacement factors ofthe PhyK structure aresmaller for thea ⁄ b domain than for the ... coli phytase, glucose-1-phosphatase and human prostatic-acid phosphatase. The polypeptidechain is organized into an a and an a ⁄ b domain, and the active site islocated in a positively charged ... substrate-freeAppA the C a atoms are 2.41 A ˚apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1.87 A ˚.Distinct conformational changes were observed...
... because ofthe absence ofthe catalyticsubunit ISP (Table 1). Figure 3A shows that a band of approximately 500 kDa was also found in this mutantstrain when the mitochondrial membranes were ana-lyzed ... were of analytical grade.Yeast strains and growth media The genotypes and sources ofthe S. cerevisiae strains aredescribed in Table 2. The ISP deletion strain wasprepared in accordance with the ... dimerization ofthe bc1complex atthis stage (i.e. just after the addition of ISP and Qcr10p).On the other hand, in the transition from the 500 kDaband to the 670 kDa band, a structural rearrangementof...
... that the calcium-binding event of lower affinity is attributable to the catalytic site of PLDa2, as found for PLDb from A. thaliana [39]. The decrease in PLD activity at concentrationsabove the ... Journal 274 (2007) 2630–2640 ª 2007 The Authors Journal compilation ª 2007 FEBS 2633from the ratio ofthe forward scattering intensity of the sample and that ofthe molecular mass standard BSA. The ab ... 2B).Reconstruction ofthe overall shape ofthe PLDa2from the X-ray scattering data was achieved by the ab initio modeling program dammin [24]. The superpo-sition ofthe 10 calculated models (Fig. 3) shows a longish...
... SPRK also has an aspar-tic acid residue at position at 200, and with the sameconformation as in the two other structures, butAsp200 forms a salt bridge to Lys253 (Ala253 andAsn250 in PRK and ... [18]. The biophysical characteri-zation of SPRK did not reveal the classical cold adap-ted features [19], but still initial comparative studiesshowed that the catalytic turnover was at least ... reported.Results and discussionCrystallization, data collection and refinementCrystals ofthe catalytic domain ofthe Serratia prote-inase K (SPRK) grew within a few days to thin hexa-gonal plates growing...
... system. Also, their underlying task (air travel domain) is much simpler than our tutoring task. In addition, the SIH is not always available and users have to activate it manually. Other visual ... hints/de-scribes the answer, each discourse segment has an additional purpose annotation that is displayed when the segment is part ofthe visible horizon. Auto-collapse. To reduce the amount of infor-mation ... also have an equivalent in a new domain (e.g. displaying the recognized user answer can be the equivalent of the correct answers). Other NM usages can also be imagined: e.g. reducing the length...
... Hospital and Regional Medical Center, Seattle, WA, USAProtein phosphatases are primarily classified on the basis ofthe type ofthe amino acid they dephosphory-late, serine ⁄ threonine phosphatases ... fused to an EF-hand motif, and human STP(HsSTP), which has an additional 8 kDa a- helicaldomain at the C-terminus [3,4]. The sizes ofthe catalytic domains of both PPP andPPM families are well ... kinase and adenylosuccinate syn-thase, but not in S. agalactiae CovR ⁄ CsrR. The motifis located at the surface ofthe SaPPase (Rantanen,unpublished), and superposition ofthe prolines allowssuperposition...
... In the report, Exhibit 3 plots the impact of average account balance and number of plan participants on the ‘all-in’ fee for a variety of combinations of average account balance and number of ... assets to arrive at the ‘all-in’ fee as a percentage of plan assets. Also, each plan’s total dollar fee amount was divided by total participants in the plan to arrive at the ‘all-in’ fee as ... further analysis found that a core specification that allowed average account balances and number of participants (both in logs) to affect fees as a percentage of assets each had separate and...
... to an increase in the rate of the initial fast phase, i.e. oxidation ofthea chains. The rates of oxidation are reduced in the presence of chelators of heavy metal cations in most cases. An ... 3A. The rate of auto-oxidation of cross-linked Hb was greaterthan that of Hb A 0,both in the presence and absence of EDTA, as has been observed previously [17]. In addition, the rates of auto-oxidation ... binding ofthe Hb tetramer to the LPSmolecule makes the heme cavity ofthea chains moreaccessible to a water molecule which can then accelerate the displacement ofthe protonated superoxide anion,...
... demonstrate the presence of positive charge.Figure 4I shows the bottom face (same as in Fig. 4C) of a van der Waals model ofthe best structureof RIIa D/D. The central part of this face appears ... two basic sites, the N-terminalamide and His2. Aspartic acids Asp27 and Asp30 showlower mean pK a values than their model pK a values. Thismay be a general property of Asp residues, as it has ... Toexamine the electrostatic character ofthestructureof RIIaD/D we have predicted mean apparent pK a values, based onPoisson–Boltzmann electrostatic calculations, using anensemble of calculated...
... compilation ª 2009 FEBS 2831 of the alanine ofthe AXXCH motif (Ala25) (ingreen) and the unsaturated vinyl group ofthe heme(cyan) are separated by 3.41 A ˚(as compared with a typical thioether ... 72% similarity. The structuresare remarkably similar overall, and in the details of the heme attachment and heme position. The rmsdbetween thestructureof C. fasciculata cytochrome cand S. ... Crystallographic data were collected atbeamline IO2 at Diamond Light Source, UK, and weacknowledge the support of T. Sorensen at Diamondand A. Labrador at the ESRF, France.References1 Barker...
... Reykjavik, Iceland), and were refined by varying the pH ofthe buffer and the concentration ofthe precipi-tant. Prior to data collection, a crystal was transferred intoParatone-N (Hampton Research) ... used as a search model. The cns program [19] was used forrefinement against the 20–2.5 A ˚intensity data. A ran-domly selected portion ofthe diffraction data (5.0%)was used to calculate the ... kinetic parameters The kinetic parameters were determined at various concen-trations ofthe substrate, tamarind seed xyloglucan (Dainip-pon Sumitomo Pharma, Osaka, Japan), in 50 mm sodiumacetate...